Search Results (391)

The saline–alkali soil environment profoundly influences the diversity and composition of soil microbial communities, reshaping their ecological network structures. As a vital staple crop, rice (Oryza sativa L.) plays a crucial role in global food security, highlighting the urgent need to improve its cultivation efficiency in saline–alkali soils. However, the mechanisms by which rice roots recruit beneficial microorganisms from native soils under prolonged saline–alkali stress remain largely unclear, and limited research has been conducted on the effectiveness of microbial inoculants in enhancing rice salt tolerance. This study investigated microbial communities in a saline field subjected to over a decade of continuous rice cultivation. Plant growth-promoting microorganisms were isolated and screened from the rhizosphere. The findings revealed long-term salt stress significantly altered microbial diversity and community composition, although the overall microbial network structure remained resilient. A total of 21 plant growth-promoting strains were identified, indicating that rice roots under sustained salt stress selectively recruit beneficial microbes that contribute to plant growth and stress adaptation. Further experimental validation demonstrated that synthetic microbial communities outperformed individual strains in promoting rice seedling growth under high-salinity conditions, likely due to synergistic microbe and microbe–plant interactions. In conclusion, while saline–alkali conditions disrupt native microbial communities, rice exhibits adaptive capacity by selectively enriching growth-promoting microorganisms. The application of synthetic microbial consortia presents a promising strategy to enhance rice resilience and productivity in saline–alkali environments. Full article

The Songnen Grassland, a typical saline–alkali ecosystem in Northeast China, is increasingly degraded by soil salinization. Arbuscular mycorrhizal fungi (AMF) are critical for enhancing plant tolerance to saline–alkali stress via root symbiosis. To investigate the species diversity and community structure of AMF in the rhizosphere of salt-tolerant plants in the Songnen Grassland, this study combined morphological identification with high-throughput sequencing (based on virtual taxa, VTs, from the MaarjAM database) to analyze the composition and distribution characteristics of AMF in the rhizosphere of eight salt-tolerant plant species, including Arundinella anomala, Leymus chinensis, Taraxacum mongolicum and others. Morphological identification revealed a total of 22 AMF species belonging to 7 genera. Among these, the genus Glomus was the dominant genus, comprising eight species (accounting for 36.4% of the total species), followed by the genus Acaulospora (five species, 22.7%), the genus Rhizophagus (four species, 18.2%), the genus Ambispora (two species, 9.1%), and the remaining genera each represented by one species (4.5%). High-throughput sequencing analysis identified a total of 40 virtual taxa (VTs) with clear taxonomic assignments belonging to six genera. The genus Glomus accounted for the highest proportion (34 VTs, 85%) with a relative abundance of 89.33%, representing the overwhelmingly dominant group. Rhizosphere soil electrical conductivity (EC) of the eight plant species indicated a significant gradient (high EC group: A–D and G, 2.07–2.61 mS/cm; low EC group: E, F, H, 0.20–0.48 mS/cm). The AMF diversity in the high EC group was significantly higher than that in the low EC group, indicating that AMF in the rhizosphere of salt-tolerant plants enhanced plant tolerance to high-salt environments, and their diversity did not decrease with increasing salinity but instead remained at a high level. Plant-specific AMF community characteristics were evident. Hierarchical clustering analysis further confirmed that the AMF community composition in the rhizosphere of Taraxacum mongolicum and Vicia amoena differed significantly from that of the other plant species, indicating that plant species have a key driving role in AMF community structure. These findings provide critical insights into the plant–AMF symbiotic mechanisms underlying saline–alkali adaptation and offer a theoretical basis for selecting efficient AMF strains to support ecological restoration of saline–alkali lands. Full article

The aim of this study was to explore the decomposition characteristics of Tamarix chinensis litter and its soil-improving capacity under different salinities. Four treatments were designed: a control (CK) treatment without saline water injection and three treatments encompassing slightly (SS, 0.4% soil salinity), moderately (SM, 0.8%), and highly saline (SH, 1.2%) conditions. T. chinensis litter at three degrees of decomposition (undecomposed, semidecomposed, and already decomposed) was studied. After 180 days, the litter substrate quality, 0–10 cm soil physicochemical properties, and enzyme activities were measured. Correlation analysis and structural equation modeling were employed to elucidate the interactions and response patterns among soil salinity, the decomposition characteristics of T. chinensis litter, and the physicochemical properties and enzyme activities of surface soil. The results revealed the following: (1) With increasing soil salinity, the contents of litter lignin, cellulose, total carbon and nitrogen residues first decreased but then increased, reaching minima under SM, whereas the content of hemicellulose residue exhibited the opposite trend. With increasing degree of litter decomposition, the contents of lignin and cellulose residues decreased, whereas the contents of hemicellulose, total nitrogen and phosphorus residues increased. (2) With increasing soil salinity, the soil water content, organic matter content, total nitrogen content, and activity of several enzymes increased, peaking under SH. The pH performance followed the order of SS > SM > CK > SH. The total carbon and phosphorus contents first increased but then decreased, with a maximum under SS. The activity of N-acetylamino glucosidase first decreased but then increased and was greatest at moderate and high salinities. (3) The soil water content and level of enzyme activity were significantly correlated with the litter substrate quality. Salinity negatively affected litter substrate residues but positively affected soil physicochemical properties. Litter decomposition under different soil salinities indirectly influenced soil enzymes by affecting soil properties, whereas salinity modulated soil properties directly or through litter decomposition. T. chinensis litter decomposition notably increased enzyme activity in moderate- to high-salinity alkali coastal soils, offering insights for low-efficiency T. chinensis forest management and saline–alkali soil remediation in the Yellow River Delta. Full article

Soil organic carbon (SOC) represents a crucial factor in agricultural production, and its accumulation is influenced by soil microbial community and microbial metabolism. Straw returning combined with decomposing agents is recognized practice to enhance SOC. On the other hand, the impacts of controlled-release nitrogen fertilizer (CR) on the function of the decomposing agent in degrading straw are underexplored. In this study, an incubation experiment with ¹³C labeled straw in three nitrogen fertilizer treatments (CK, no nitrogen applied; UR, urea applied; CR, controlled-release fertilizer applied) was carried out to elucidate how CR regulates the straw decomposition agent and bacterial community to influence the SOC sequestration, based on field experiments. And we examined the changes in soil organic carbon and the stability of the bacterial networks by combining co-occurrence networks and a structural equation model. In the incubation experiment, the results demonstrated that CR increased the relative abundance of straw decomposition agent and straw-derived SOC (SO¹³C). Additionally, CR enhanced the stability of soil bacterial networks, compared with UR, by strengthening the interactions within the soil bacterial community. Pearson correlations confirmed that straw decomposition agent was positively associated with SO¹³C. Moreover, the straw decomposition agent was positively correlated with the activities of the nitrogen-cycling enzyme (urease, N-acetyl-β-glucosaminidase) and carbon-degrading enzyme (β-1,4-glucosidase, cellulase). Furthermore, structural equation modeling indicated that soil inorganic nitrogen played the most direct role in changes in the straw decomposition agent and then indirectly stimulated the activity of cellulase, ultimately increasing straw-derived carbon in the soil. This study elaborates the mechanism of straw returning combined with straw decomposition agent and controlled-release fertilizers to enhance the SOC of coastal saline–alkali soil from the perspective of underground biology. Collectively, the results of this research might improve the management of straw returning and sustainable utilization of fertility in saline–alkali soil. It provides a new perspective on fertilization for increasing soil carbon sequestration in future farmland ecosystems. Full article

In order to identify saline–alkali-tolerant rapeseed varieties suitable for cultivation on moderately saline–alkali soils and to expand the use of such lands, six rapeseed varieties were selected as experimental materials. Field experiments were conducted to evaluate agronomic traits, photosynthesis, stress physiology, yield, and quality throughout the entire growth period. Statistical methods, including correlation analysis, principal component analysis, membership function analysis, and cluster analysis, were employed to evaluate and select saline–alkali-tolerant varieties. The results indicated that H62 and 20C14 yielded the highest seed production, reaching 2287.99 kg·hm⁻² and 2277.15 kg·hm⁻², respectively. During the mid-to-late growth stages, the majority of agronomic traits, photosynthetic parameters, and stress physiology indicators for 20C14 were significantly superior to those of the other varieties. The results of the principal component analysis showed that the total root length at maturity stage, root–shoot ratio at flowering stage, and proline content at maturity stage were the most important indicators for screening saline–alkali-tolerant rapeseed varieties. A comprehensive analysis of these indicators revealed the following descending order of saline–alkali tolerance among the varieties: 20C14 > 20C17 > 20C4 > H62 > H158 > 17C2. Cluster analysis was performed to classify the rapeseed into strong saline–alkali-tolerant type (20C14 and 20C17), moderate saline–alkali-tolerant type (20C4, H62, and H158), and weak saline–alkali-tolerant type (17C2). Consequently, 20C14 and 20C17 are recommended as suitable rapeseed varieties for cultivation on soda saline–alkali soils. Full article

Microbial reduction in hexavalent chromium (Cr(VI)) is a well characterized bioremediation strategy, yet the mechanistic diversity among bacterial taxa necessitates detailed investigations into strain-specific pathways. Here, we report the isolation and characterization of Bacillus safensis BSF-4, a halophilic bacterium derived from saline-alkali soil, which demonstrates efficient Cr(VI) reduction capacity. Physiological assays showed that BSF-4 achieved 89.15% reduction of 20 mg/L Cr(VI) within 72 h, with Cr(III) identified as the primary extracellular end product. Resting cell assays and subcellular fractionation analyses confirmed that Cr(VI) reduction predominantly occurs in the extracellular milieu. X-ray photoelectron spectroscopy (XPS) further revealed soluble Cr(III) complexed with extracellular polymeric substances (EPS). Transcriptomic profiling indicated upregulation of membrane-associated transport systems (facilitating Cr(VI) exclusion) and quorum sensing (QS) pathways (mediating adaptive stress responses). These findings highlight a dual mechanism: (1) extracellular enzymatic reduction mediated by EPS-bound redox proteins, and (2) intracellular detoxification via QS-regulated defense pathways. Collectively, Bacillus safensis BSF-4 exhibits robust Cr(VI) reduction capacity under saline conditions, positioning it as a promising candidate for bioremediation of Cr(VI)-contaminated saline soils and aquatic ecosystems. Full article

China, facing severe saline–alkali land degradation, is grappling with the paradox of technically adequate but systemically deficient land consolidation. In response to the existing evaluation system’s over-reliance on physicochemical indicators and neglect of socioeconomic value, this study proposes the use of the Optimal Land Use Value (OLV) to construct a comprehensive benefit evaluation indicator system for saline–alkali land consolidation that encompasses ecosystem resilience, supply–demand balancing, and common prosperity. Considering a case project implemented from 2019 to 2022 in the Western Songnen Plain of China—one of the world’s most severely affected soda saline–alkali regions—this study combines the land use transition matrix with a comprehensive evaluation model to systematically assess the effectiveness and sustainability of land consolidation. The results reveal systemic deficiencies: within ecological spaces, short-term desalination succeeds but pH and organic matter improvements remain inadequate, while ecosystem vulnerability increases due to climate fluctuations and grassland conversion. In production spaces, cropland expansion and saline land reduction are effective, but water resource management proves unsustainable. Living spaces show improved infrastructure and income but face threats due to economic simplification and intergenerational unsustainability. For the investigated case, recommendations include shifting from technical restoration to systemic governance via three strategies: (1) biological–engineering synergy employing green manure to enhance soil microbial activity; (2) hydrological balancing through groundwater quotas and rainwater utilization; (3) specialty industry development for rural economic diversification. This study contributes empirical evidence on the conversion of saline–alkali land, as well as an evaluation framework of wider relevance for developing countries combating land degradation and pursuing rural revitalization. Full article

Background: Lespedeza davurica is an important perennial leguminous shrub endemic to China’s Loess Plateau, and it plays a crucial role in ecosystem restoration and soil erosion control. However, phosphorus deficiency and environmental stresses limit its growth potential and ecological function. Methods: In the present study, the interaction between Acinetobacter calcoaceticus DP25, a phosphate-solubilizing rhizobacterium isolated from L. davurica rhizosphere, and L. davurica was investigated. We performed biochemical analyses of leaves from L. davurica planted in saline–alkali soil to monitor antioxidant defense systems and stress-related metabolites, and conducted a combination of transcriptomics and metabolomics approaches to elucidate the bacteria-mediated enhancement of growth and stress tolerance in L. davurica. Results: DP25 inoculation substantially enhanced L. davurica growth performance, increasing plant height by 47.68%, biomass production by 102.54–132.42%, and root architecture parameters by 62.68–78.79% (p < 0.0001). Catalase activity, a key antioxidant enzyme, showed a marked increase of 41.53% (p < 0.001), while malondialdehyde and free proline contents decreased by 18.13% and 19.33%, respectively (p < 0.05). Transcriptomic analysis revealed 263 differentially expressed genes, with enrichment in carotenoid biosynthesis, ABC transporters, and pentose and glucuronate interconversion pathways. Metabolomic profiling identified 246 differentially accumulated metabolites, highlighting enhanced secondary metabolite production and stress response mechanisms. Integration of multi-omics data revealed 19 co-regulated pathways involved in growth promotion and stress tolerance. Conclusions: A. calcoaceticus DP25 enhances L. davurica growth through coordinated regulation of metabolic pathways involved in photosynthesis, antioxidant defense, and secondary metabolite biosynthesis. These findings provide molecular insights into beneficial plant–microbe interactions and support the development of sustainable strategies for ecosystem restoration in degraded environments. Full article

As an important reserve resource for cultivated land, the improvement and fertility enhancement of saline-alkali land are key to alleviating the pressure on cultivated land and ensuring the sustainable utilization of land resources. Studying the regulatory effect of rotation patterns on the soil fertility of saline-alkali land is one of the core research contents in exploring low-cost and environmentally friendly comprehensive management strategies for saline-alkali land. This study focuses on Zhaoyuan County, a representative saline and alkaline area within the Songnen Plain. Utilizing remote sensing technology, crop information was systematically collected across 13 time periods spanning from 2008 to 2020. These data were employed to construct a comprehensive crop information change atlas. This atlas categorized crop rotation patterns based on crop combinations, rotation frequencies, and the number of consecutive years of planting. Using soil sampling data from 2008 and 2020, a soil fertility evaluation was conducted, and the changes in soil chemical properties and fertility under various crop rotation patterns were analyzed. The results of the study show that, during the study period, crop rotation patterns in Zhaoyuan County were dominated by paddy-upland rotations and upland crop rotations. Crop rotation patterns, categorized by crop combination, were dominated by soybean–maize–other crops rotation (S-M-O) and rice–soybean–maize–other crops rotation (R-S-M-O). The frequency of crop rotation is dominated by low- and medium-frequency crop rotation. Crop rotation significantly increased soil organic matter, total nitrogen content, and overall soil fertility in the study area, while simultaneously lowering soil pH levels. Crop rotation patterns with different crop combinations had significant effects on soil chemical properties, with smaller differences in the effects of different rotation frequencies and years of continuous cropping. Crop rotation patterns incorporating soybean demonstrate a significant positive regulatory impact on the soil fertility of saline-alkali land. Low-frequency crop rotation (with ≤5 crop changes) has a relatively better effect on improving soil fertility. This research provides important empirical support and decision-making references for establishing sustainable farming systems in ecologically fragile saline-alkali areas, ensuring regional food security, and promoting the long-term sustainable utilization of land resources. Full article

Desert ecosystems pose extreme challenges to plant survival. This study explores the adaptive strategies of two xerophytic halophytes, Alhagi sparsifolia and Nitraria roborowskii, in Xinjiang’s Ebinur Lake wetland, focusing on their plant–soil–microbe (PSM) coupling systems across desert gradients. Results revealed significant interspecific and gradient-dependent differences in plant functional traits: A. sparsifolia showed high growth plasticity with a fast-growth strategy, while N. roborowskii adopted a conservative strategy. Rhizosphere soil physicochemical properties and microbial community structure exhibited strong spatial heterogeneity and host specificity, with N. roborowskii having a more complex microbial network and A. sparsifolia showing higher modularity. Multivariate factor analysis elucidated couplings among plant traits, soil properties, enzymes, and microbes. The two species form distinct interaction systems adapted to desert saline–alkali stress, advancing the understanding of ecological adaptation and informing restoration. Full article

Soil salinization limits the growth of agricultural crops in the world, requiring the use of methods to increase the tolerance of agricultural crops to salinity–alkali stress. Arbuscular mycorrhizal fungi (AMF) enhance plant stress adaptation through symbiosis and offer a promising strategy for remediation. However, in non-model crops such as oat (Avena sativa L.), research has mainly focused on physiological assessments, while the key genes and metabolic pathways involved in AMF-mediated growth and saline–alkali tolerance remain unclear. In this study, we employed integrated multi-omics and physiological analyses to explore the regulatory mechanisms of AMF in oats under normal and saline–alkali stress. The results indicated that AMF symbiosis significantly promoted oat growth and physiological performance under both normal and saline–alkali stress conditions. Compared to the non-inoculated group under normal conditions, AMF increased plant height and biomass by 8.5% and 15.3%, respectively. Under saline–alkali stress, AMF enhanced SPAD value and relative water content by 16.7% and 7.3%, reduced MDA content by 35.8%, increased soluble protein by 21.8%, and decreased proline by 13.3%. In addition, antioxidant enzyme activities (SOD, POD, and CAT) were elevated by 18.4%, 18.2%, and 14.8%, respectively. Transcriptomic analysis revealed that AMF colonization under saline–alkali stress induced about twice as many differentially expressed genes (DEGs) as under non-saline–alkali stressed conditions. These DEGs were primarily associated with Environmental Information Processing, Genetic Information Processing, and Metabolic Processes. According to metabolomic analysis, a total of 573 metabolites were identified across treatments, mainly comprising lipids (29.3%), organic compounds (36.8%), and secondary metabolites (21.5%). Integrated multi-omics analysis indicated that AMF optimized energy utilization and antioxidant defense by enhancing phenylpropanoid biosynthesis and amino acid metabolism pathways. This study provides new insights into how AMF may enhance oat growth and tolerance to saline–alkali stress. Full article

Glycyrrhiza uralensis (G. uralensis), a leguminous plant, is an important medicinal and economic plant in saline–alkaline soils of arid regions in China. Its main bioactive components include liquiritin, glycyrrhizic acid, and flavonoids, which play significant roles in maintaining human health and preventing and adjuvantly treating related diseases. However, the cultivation of G. uralensis is easily restricted by adverse soil conditions in these regions, characterized by high salinity, high alkalinity, and nutrient deficiency. This study investigated the impacts of four multistrain microbial inoculants (Pa, Pb, Pc, Pd) on the growth performance and bioactive compound accumulation of G. uralensis in moderately saline–sodic soil. The aim was to screen the most beneficial inoculant from these strains, which were isolated from the rhizosphere of plants in moderately saline–alkaline soils of the Hexi Corridor and possess native advantages with excellent adaptability to arid environments. The results showed that inoculant Pc, comprising Pseudomonas silesiensis, Arthrobacter sp. GCG3, and Rhizobium sp. DG1, exhibited superior performance: it induced a 0.86-unit reduction in lateral root number relative to the control, while promoting significant increases in single-plant dry weight (101.70%), single-plant liquiritin (177.93%), single-plant glycyrrhizic acid (106.10%), and single-plant total flavonoids (107.64%). Application of the composite microbial inoculant Pc induced no significant changes in the pH and soluble salt content of G. uralensis rhizospheric soils. However, it promoted root utilization of soil organic matter and nitrate, while significantly increasing the contents of available potassium and available phosphorus in the rhizosphere. High-throughput sequencing revealed that Pc reorganized the rhizospheric microbial communities of G. uralensis, inducing pronounced shifts in the relative abundances of rhizospheric bacteria and fungi, leading to significant enrichment of target bacterial genera (Arthrobacter, Pseudomonas, Rhizobium), concomitant suppression of pathogenic fungi, and proliferation of beneficial fungi (Mortierella, Cladosporium). Correlation analyses showed that these microbial shifts were linked to improved plant nutrition and secondary metabolite biosynthesis. This study highlights Pc as a sustainable strategy to enhance G. uralensis yield and medicinal quality in saline–alkali ecosystems by mediating microbe–plant–nutrient interactions. Full article

Soil salinization and selenium (Se) deficiency threaten global food security. This study developed a composite bioinoculant combining ACC deaminase-producing Serratia liquefaciens and biogenically synthesized nano-selenium (SeNPs) to alleviate saline–alkali stress and enhance Se nutrition in rice (Oryza sativa L.). A strain of S. liquefaciens with high ACC deaminase activity was isolated and used to biosynthesize SeNPs with stable physicochemical properties. Pot experiments showed that application of the composite inoculant (S3: S. liquefaciens + 40 mmol/L SeNPs) significantly improved seedling biomass (fresh weight +53.8%, dry weight +60.6%), plant height (+31.6%), and root activity under saline–alkali conditions. S3 treatment also enhanced panicle weight, seed-setting rate, and grain Se content (234.13 μg/kg), meeting national Se-enriched rice standards. Moreover, it increased rhizosphere soil N, P, and K availability and improved microbial α-diversity. This is the first comprehensive demonstration that a synergistic bioformulation of ACC deaminase PGPR and biogenic SeNPs effectively mitigates saline–alkali stress, enhances soil fertility, and enables safe Se biofortification in rice. Full article

Soil salinization poses a significant constraint to agricultural productivity. However, certain plant growth-promoting bacteria (PGPB) can mitigate salinity stress and enhance crop performance. In this study, a bacterial isolate, R-18, isolated from saline-alkali soil in Ningxia, China, was identified as Enterobacter bugandensis based on 16S rRNA gene sequencing. The isolate was characterized for its morphological, biochemical, and plant growth-promoting traits and was evaluated for its potential to alleviate NaCl-induced stress in maize (Zea mays L.) under hydroponic conditions. Isolate R-18 exhibited halotolerance, surviving at NaCl concentrations ranging from 2.0% to 10.0%, and alkaliphilic adaptation, growing at pH 8.0–11.0. Biochemical assays confirmed it as a Gram-negative bacterium, displaying positive reactions in the Voges–Proskauer (V–P) tests, catalase activity, citrate utilization, fluorescent pigment production, starch hydrolysis, gelatin liquefaction, and ammonia production, while testing negative for the methyl red and cellulose hydrolysis. Notably, isolate R-18 demonstrated multiple plant growth-promoting attributes, including nitrogen fixation, phosphate and potassium solubilization, ACC deaminase activity, and indole-3-acetic acid (IAA) biosynthesis. Under 100 mM NaCl stress, inoculation with isolate R-18 significantly enhanced maize growth, increasing plant height, stem dry weight, root fresh weight, and root dry weight by 20.64%, 47.06%, 34.52%, and 31.25%, respectively. Furthermore, isolate R-18 improved ion homeostasis by elevating the K⁺/Na⁺ ratio in maize tissues. Physiological analyses revealed increased chlorophyll and proline content, alongside reduced malondialdehyde (MDA) levels, indicating mitigated oxidative damage. Antioxidant enzyme activity was modulated, with decreased superoxide dismutase (SOD) and peroxidase (POD) activities but increased catalase (CAT) activity. These findings demonstrated that Enterobacter bugandensis R-18 effectively alleviated NaCl-induced growth inhibition in maize by enhancing osmotic adjustment, reducing oxidative stress, and improving ion balance. Full article

Soil salinity adversely affects crop growth and development, leading to reduced soil fertility and agricultural productivity. The indigenous salt-tolerant plant growth-promoting rhizobacteria (PGPR), as a sustainable microbial resource, do not only promote growth and alleviate salt stress, but also improve the soil microecology of crops. The strain H5 isolated from saline-alkali soil in Bachu of Xinjiang was studied through whole-genome analysis, functional annotation, and plant growth-promoting, salt-tolerant trait gene analysis. Phylogenetic tree analysis and 16S rDNA sequencing confirmed its classification within the genus Halomonas. Functional annotation revealed that the H5 genome harbored multiple functional gene clusters associated with plant growth promotion and salt tolerance, which were critically involved in key biological processes such as bacterial survival, nutrient acquisition, environmental adaptation, and plant growth promotion. The pot experiment under moderate salt stress demonstrated that seed inoculation with Halomonas sp. H5 not only significantly improved the agronomic traits of tomato seedlings, but also increased plant antioxidant enzyme activities under salt stress. Additionally, soil analysis revealed H5 treatment significantly decreased the total salt (9.33%) and electrical conductivity (8.09%), while significantly improving organic matter content (11.19%) and total nitrogen content (10.81%), respectively (p < 0.05). Inoculation of strain H5 induced taxonomic and functional shifts in the rhizosphere microbial community, increasing the relative abundance of microorganisms associated with plant growth-promoting and carbon and nitrogen cycles, and reduced the relative abundance of the genera Alternaria (15.14%) and Fusarium (9.76%), which are closely related to tomato diseases (p < 0.05). Overall, this strain exhibits significant potential in alleviating abiotic stress, enhancing growth, improving disease resistance, and optimizing soil microecological conditions in tomato plants. These results provide a valuable microbial resource for saline soil remediation and utilization. Full article