Search Results (264)

In vitro culture has become a key tool to produce seed potato (Solanum tuberosum L.) tubers, a crop of great global importance. This systematic review, based on the PRISMA-ScR methodology, analyzes the main biotechnological strategies used to obtain high-quality, healthy, and pathogen-free seeds, overcoming the limitations of traditional propagation methods. A comprehensive search was conducted in Scopus, Web of Science, and ScienceDirect (June 2025), prioritizing 65 experimental studies published between 2010 and 2025 in indexed journals. Techniques such as meristem culture, micropropagation, microtuber production, the use of temporary immersion bioreactor systems (TIBs), and synthetic seed generation were examined. These methodologies offer advantages such as accelerated propagation, higher yields, reduced use of agrochemicals, germplasm conservation, and economic efficiency. TIBs stand out for improving the survival and productivity of basic seed. This review is organized around four axes: applied techniques, key procedures, economic impact and sustainability, and future perspectives. This work constitutes a useful guide for optimizing seed tuber production using plant biotechnology. Full article

Despite the continued growth of the gluten-free food market, there is a dearth of sensory and consumer knowledge on commercial products. The existing research is mostly limited to hedonic measurements and ingredient effects instead of analytical methods for a better understanding of product characteristics of gluten-free crackers specifically. In this work, a semi-trained consumer panel used projective mapping to choose objectively different plain/original crackers from a pool of sixteen commercial gluten-free cracker varieties. The cracker samples represented a widespread sensory space originating from different key ingredients such as brown rice, white rice, flaxseed, cassava flour, nut flour blend, millet blend, and tapioca/potato starch blend. Based on projective mapping results, the crackers that mostly represented the sensory space were selected for characterization by a modified flash profiling method. The consumer panel developed 74 descriptors: 30 aromas, 28 flavors, 15 texture terms, and a mouthfeel attribute. The samples were monadically rated for intensity on a 4-point scale (0 = none, 1 = low, 2 = medium, and 3 = high). Rice, toasted, salt, grain, burnt, flaxseed, bitter, earthy, nutty, seeds, and grass were the prevalent aromas and flavors. Others were specific to cracker type. Some of these attributes can be traced back to the ingredients list. Results suggest that ingredients used in small portions are defining the flavor properties over the major grains/flour blends. All samples had some degree of crunchiness, crispness, and pasty mouthfeel; rice crackers were particularly firm, hard, and chewy; brown rice crackers were gritty; crackers with tuber starches/flours were more airy, soft, smooth, and flaky. Overall, the samples shared more aroma and flavor notes than texture attributes. In comparison to trained panel results, consumers generated a greater number of terms and were successful in finding subtle differences primarily in texture but had many overlapped flavors. The developed consumer terminology will facilitate the gluten-free industry to tailor communication that better resonates with consumer experiences, needs, and product values. Full article

This study investigated the effects of light intensity regulation on yield and energy efficiency during potato pre-basic seed propagation in plant factories. Using virus-free ‘Favorita’ potato seedlings as experimental material, gradient light intensities (200, 300, and 400 μmol·m²·s⁻¹) were applied at four developmental stages: the seedling stage (SS), tuber formation stage (TFS), tuber growth stage (TGS), and harvest stage (HS), to explore the physiological mechanisms of stage-specific light intensity regulation and energy utilization efficiency. The results revealed that: (1) The per-plant tuber yield of the high yield group reached 72.91 g (T59 treatment), representing a 25% increase compared to the medium yield group and a 168% increase compared to the low yield group. Additionally, the high yield group exhibited superior leaf area, photosynthetic rate, and accumulation of sucrose and starch. (2) The impact of light intensity on tuber development exhibited stage specificity: low light intensity (200 μmol·m⁻²·s⁻¹) during TFS promoted early tuber initiation, while a high light intensity (400 μmol·m⁻²·s⁻¹) enhanced tuber formation efficiency. Increasing the light intensity during TGS facilitated the accumulation of sucrose and starch in tubers. (3) Energy use efficiency (EUE) increased significantly with yield, with the high yield group reaching 3.2 g MJ⁻¹, representing 52% and 88% improvements over the medium yield (2.1 g MJ⁻¹) and low yield (1.7 g MJ⁻¹) groups, respectively. A “stage-specific precision light supplementation” strategy was proposed, involving moderate light reduction (200 μmol·m⁻²·s⁻¹) during TFS and light enhancement (300 μmol·m⁻²·s⁻¹) during TGS to coordinate source-sink relationships and optimize carbohydrate metabolism. This study provides a theoretical basis for efficient potato production in plant factories. Full article

China ranks as the world’s leading potato (Solanum tuberosum L.) producer, while the poor seeding machinery performance limited a higher input–output ratio in potato cultivation and impeded sustainable development. We developed an advanced air-suction mulai-arm potato planter (ASPP) that incorporated integrated side-deep fertilization, automated seed feeding, negative-pressure seed filling, seed transportation, positive-pressure seed delivery, soil covering, and compaction. The study proposes a Negative-pressure seed extraction mechanism that minimizes seed damage by precisely controlling suction pressure, and the near-zero-speed seed delivery mechanism synchronizes seed release with ground speed, reducing bounce-induced spacing errors. Furthermore, the structural configuration and operation principle of ASPP were systematically elucidated, and key performance parameters and optimal values were identified. We conducted a randomized complete block design plot trial comparing the spoon-belt potato planter (SBPP) and spoon-chain potato planter (SCPP), evaluating sowing quality, seedling emergence rate (ER), potato yield (PY), and comprehensive economic benefits. The results revealed that plant spacing index (PSI), missed-seeding index (MI), re-seeding index (RI), and coefficient of variation (CV) of ASPP were 90.05%, 3.78%, 2.32%, and 7.93%, respectively. The mean ER values for ASPP, SBPP, and SCPP were 94.76%, 85.42%, and 83.46%, respectively, with the ASPP showing improvements of 10.93% and 13.54% over SBPP and SCPP. However, the SBPP and SCPP exhibited greater emergence uniformity than ASPP. The mean PY value was 37,205.25, 32,973.75, and 34,620 kg·ha⁻¹ for ASPP, SBPP, and SCPP. The ASPP outperformed the SBPP and SCPP by 12.83% and 7.47%. Overall, ASPP demonstrated balanced and superior performance across the above-mentioned indicators, demonstrating its potential to enable precision agriculture in tuber crop cultivation. Full article

Aiming at the problems of the high multiple- and missed-seeding index and low operation efficiency of current mechanical potato seed-meters in minituber sowing, this study designed an air-suction roller-type minituber seed-metering device for minitubers (mass between 2 and 4 g) in accordance with the agronomic standards for potato cultivation in the single-cropping area of northern China. An account of the device’s structure and operational principle was made, its working process was theoretically analysed, and the three main factors affecting the airflow suction were determined: the seed roller speed, the suction seeding hole diameter, and the air inlet negative pressure. This study adopted the fluid dynamics simulation method and determined that the ideal location of the air inlet was 30° for horizontal inclination and 60° for vertical inclination. Then, based on the CFD-DEM fluid-structure coupling simulation method, the impact of a range of factors on the functionality of the seed-metering device under different conditions was studied and verification tests were carried out. Design-Expert was used to analyse test results. The results showed that when the pressure at the air inlet was −7000 Pa, the speed of the seeding roller was 40.2 r·min⁻¹, the suction seeding hole diameter was 10.37 mm, and the performance was optimal: the qualified index was 92.95%, the multiple-seeding index was 4.16%, and the missed-seeding index was 2.89%. The research results show that the seed-metering device developed under this scheme exhibited satisfactory seeding performance during operation and was able to meet the demands of minituber sowing. Full article

An efficient in vitro propagation protocol for Eulophia bicallosa was developed using asymbiotic seed germination and protocorm proliferation. The effect of light on seed germination and development was evaluated on Vacin and Went (VW) medium under five conditions: darkness, white, green, red, and blue light for 24 weeks. Blue and red light significantly accelerated seed development, allowing progression to stage 5 within 24 weeks. For protocorm proliferation, six semi-solid culture media were tested. Half-strength Murashige and Skoog (½MS) medium yielded the best results after 8 weeks, producing the highest numbers of shoots (1.0), leaves (1.1), and roots (4.2) per protocorm, with 100% survival. The effects of organic additives were also evaluated using coconut water and potato extract. A combination of 200 mL L⁻¹ coconut water and 50 g L⁻¹ potato extract enhanced shoot formation (1.7 shoots), while 150 mL L⁻¹ coconut water with 50 g L⁻¹ potato extract increased both leaf (1.9) and root (8.8) numbers. The effects of cytokinins (benzyladenine (BA), kinetin (6-furfurylaminopurine), and thidiazuron (TDZ)) and auxins (indole-3-acetic acid (IAA), α-naphthalene acetic acid (NAA), indole-3-butyric acid (IBA), and 2,4-dichlorophenoxyacetic acid (2,4-D)) were investigated using ½MS medium supplemented with each plant growth regulator individually at concentrations of 0, 0.1, 0.5, 1.0, and 2.0 mg L⁻¹. Among the cytokinins, 0.1 mg L⁻¹ BA produced the highest survival rate (96%), while 1.0 mg L⁻¹ BA induced the greatest shoot formation (93%, 2.3 shoots). Among the auxins, 0.1 mg L⁻¹ IAA resulted in the highest survival (96%), and 1.0 mg L⁻¹ IAA significantly enhanced root induction (4.2 roots per protocorm). Acclimatization in pots containing a 1:1:1 (v/v) mixture of pumice, sand, and soil resulted in 100% survival. This protocol provides a reliable and effective approach for the mass propagation and ex situ conservation of E. bicallosa. Full article

Insecticides are mainly used to control aphids as they are potatoes’ main vectors of viruses. This study analysed the flight activity of Myzus persicae, Phorodon humuli, and Aphis nasturtii from a suction trap over 22 years (2002–2024). We also analysed the flight activity of seven aphid species, vectors of viruses from the yellow water trap over 6 years (2019–2024). The number of catches of aphids in the suction trap was higher in 2014–2024 than in 2002–2013: for M. persicae, 4.2-fold, P. humuli 2.1-fold, and A. nastrurtii, 1.9-fold. A statistically significant correlation between mean temperature per year and total capture of M. persicae per year in the suction trap was found. The analysis showed no relationship between the abundance of M. persicae and P. humuli from the suction trap in spring and the abundance in the yellow water trap in the potato field. The dominant aphid species in the yellow water trap were M. persicae, Brevicoryne brassicae, and Aphis fabae. Regression analysis showed no direct relationship between aphid abundance in the yellow water trap over the period of 2019–2024 and seedling recruitment. Potato aphid control options are discussed concerning the flight activity and specific life cycles of each aphid species. Full article

The effects of wound healing on crop stand and productivity were examined on the potato (Solanum tuberosum L.) cultivars Alturas (Alt), Russet Burbank (RB), and Clearwater Russet (CW). Tuber yields increased linearly with an advancing wound healing period irrespective of the cultivar (R² = 0.91). In contrast to unhealed controls, RB and CW wound-healed for 8 days produced a 6% and 8% greater yield, respectively, while a shorter wound healing period of 2 days increased Alt yield by 7%. Increases in tuber yield, a consequence of enhanced specific tuber weight across wound healing periods, contributed towards increased relative crop value in Alt (13%), RB (22%), and CW (19%). In further lab evaluations, Alt exhibited increased desiccation resistance and was associated with an earlier induction (24 h post-wounding) of feruloyl transferase (FHT) compared to CW and RB. Since FHT facilitates suberin and wax development, delayed FHT induction likely promoted fresh-weight loss in CW and RB compared to Alt. Enzymatic evaluations to assess the production of reactive oxygen species to protect fresh-cut seed found that RB had the highest activities of superoxide dismutase and peroxidase. This study demonstrates the broad benefits of planting wound-healed seed while highlighting opportunities to improve best practices and genetic improvement for wound healing response. Full article

The potato is the third most important crop in the world, and more than 375 million metric tonnes of potatoes are produced globally on an annual basis. Potato Virus Y (PVY) poses a significant threat to the production of seed potatoes, resulting in economic losses and risks to food security. Current detection methods for PVY typically rely on serological assays for leaves and PCR for tubers; however, these processes are labor-intensive, time-consuming, and not scalable. In this proof-of-concept study, we propose the use of unmanned aerial vehicles (UAVs) integrated with hyperspectral cameras, including a downwelling irradiance sensor, to detect the PVY in commercial growers’ fields. We used a 400–1000 nm visible and near-infrared (Vis-NIR) hyperspectral camera and trained several standard machine learning and deep learning models with optimized hyperparameters on a curated dataset. The performance of the models is promising, with the convolutional neural network (CNN) achieving a recall of 0.831, reliably identifying the PVY-infected plants. Notably, UAV-based imaging maintained performance levels comparable to ground-based methods, supporting its practical viability. The hyperspectral camera captures a wide range of spectral bands, many of which are redundant in identifying the PVY. Our analysis identified five key spectral regions that are informative in identifying the PVY. Two of them are in the visible spectrum, two are in the near-infrared spectrum, and one is in the red-edge spectrum. This research shows that early-season PVY detection is feasible using UAV hyperspectral imaging, offering the potential to minimize economic and yield losses. It also highlights the most relevant spectral regions that carry the distinctive signatures of PVY. This research demonstrates the feasibility of early-season PVY detection using UAV hyperspectral imaging and provides guidance for developing cost-effective multispectral sensors tailored to this task. Full article

Seed potatoes without sprouts usually need to be manually selected in mechanized production, which has been the bottleneck of efficiency. A fast and efficient object recognition algorithm is required for the additional removal process to identify unqualified seed potatoes. In this paper, a lightweight deep learning algorithm, YOLOv8_EBG, is proposed to both improve the detection performance and reduce the model parameters. The ECA attention mechanism was introduced in the backbone and neck of the model to more accurately extract and fuse sprouting features. To further reduce the model parameters, Ghost convolution and C3ghost were introduced to replace the normal convolution and C2f blocks in vanilla YOLOv8n. In addition, a bi-directional feature pyramid network is integrated in the neck part for multi-scale feature fusion to enhance the detection accuracy. The experimental results from an isolated test dataset show that the proposed algorithm performs better in detecting sprouts under natural light conditions, achieving an mAP_0.5 of 95.7% and 91.9% AP for bud recognition. Compared to the YOLOv8n model, the improved model showed a 6.5% increase in mAP_0.5, a 12.9% increase in AP_0.5 for bud recognition, and a 5.6% decrease in the number of parameters. Additionally, the improved algorithm was applied and tested on mechanized sorting equipment, and the accuracy of seed potato detection was as high as 92.5%, which was sufficient to identify and select sprouted potatoes, an indispensable step since only sprouted potatoes can be used as seed potatoes. The results of the study can provide technical support for subsequent potato planting intelligence. Full article

Background: While unhealthy dietary trends, such as elevated confectionery consumption and decreased fruit intake, have been documented in adults, the longitudinal patterns of food consumption during childhood remain inadequately characterized. This study aimed to describe national trends in food group intakes among children and adolescents in Japan. Methods: Data on participants aged 1–19 years in the National Health and Nutrition Survey from 2001 to 2019 (n = 37,072) were used in this study. A 1-day, semi-weighed, household-based dietary record was used to assess dietary intake. Results: Decreasing trends in the intakes of animal-based foods, potatoes and starches, sugars and sweeteners, fruits (annual percentage change [APC] range: −5.04 to −0.62), algae, fish and shellfish (APC range: −3.22 to −2.02), eggs, milks, fats and oils, and confectionery were observed, while intakes of meats (APC range: 1.02 to 1.92) and beverages (APC range: 1.36 to 2.51) increased. Consumption of plant-based foods, cereals, legumes, nuts and seeds, vegetables, and mushrooms was mostly unchanged, whereas variable intakes of seasonings and spices were observed. Conclusions: This study showed that the intakes of many food groups (e.g., fruits, fish and shellfish, and milk) decreased among children and adolescents, but some increased (e.g., meats and beverages) or remained stable (e.g., cereals and vegetables). Further evidence is needed to enable policymakers to set target interventions to improve children’s diets. Full article

The effects of the combination of the light intensity and photoperiod on the yield of virus-free potato and resource utilization are still poorly understood, especially under the same daily light integral. Here, we tested the responses of virus-free potato in terms of plant growth, photosynthesis, and the tuber yield to four light intensity and photoperiod combinations (T1, 200 μmol m⁻² s⁻¹ and 16.5 h; T2, 300 μmol m⁻² s⁻¹ and 11 h; T3, 400 μmol m⁻² s⁻¹ and 8.25 h; T4, 500 μmol m⁻² s⁻¹ and 6.6 h) under the same daily light integral. The tuber number and dry weight increased with the shortening of the photoperiod. The smaller number of tubers under the T1 treatment was due to the poor transport of photosynthates in the leaves and the uneven distribution of photosynthates in the tubers during the tuber formation stage. Changes in the light intensity and photoperiod combination did not significantly impact the distribution ratio of photosynthates between plants and tubers at the tuber growth stage; they only caused adjustments in the total amount of photosynthates. In addition, the photoinhibition under T1 was caused by the long photoperiod and that under T4 was caused by the high light intensity. However, the higher content of carotene in the T4 treatment alleviated the adverse effects of photoinhibition on the tuber yield. The results showed that the number and growth of tubers were extremely negatively correlated with shoot growth and extremely positively correlated with the accumulation rate of photosynthates in the plant and the tubers per day. In terms of energy consumption and the cost of the light source, we recommend the light intensity and photoperiod combinations of 300 μmol m⁻² s⁻¹ and 11 h or 400 μmol m⁻² s⁻¹ and 8.25 h to cultivate virus-free potato in plant factories. Full article

China, the largest global producer of sweet potatoes, faces significant threats from viral diseases, particularly in Fujian Province, where sweet potatoes are the second most important food crop after rice. This study identified 11 viruses, including sweet potato feathery mottle virus (SPFMV) and sweet potato chlorotic stunt virus (SPCSV), infecting sweet potatoes in Fujian. Sequence comparisons revealed diverse strains from various sources. Virus prevalence varied across regions, with Quanzhou, Fuzhou, and Putian severely affected, detecting 10, 9, and 7 viruses, respectively, compared to only 3 in Sanming and Longyan. In particular, sweet potato virus disease (SPVD) caused the most severe damage during the seeding stages, resulting in dwarfing and leaf deformation, while the damage was lighter during the growth period, manifesting as the yellowing and brittleness of the leaves, ultimately reducing the yield. Compound infestations predominated, with between 0 and 6 viruses infecting different sweet potato varieties. Single-virus infections were observed for sweet potato virus 2 (SPV2), sweet potato symptomless virus 1 (SPSMV-1), and sweet potato pakakuy virus (SPPV), while others, particularly SPCSV, were frequently co-infected with SPFMV, leading to SPVD development. Further analysis showed that the RNase3 expression of SPCSV was correlated with the SPVD severity in sweet potato. These findings provide insights into the epidemiology of sweet potato viruses and serve as a reference for developing targeted disease management strategies. Full article

In China, potatoes are predominantly cultivated using the tuber piece planting method. During the cutting process, it is essential to divide seed potatoes into tuber pieces based on the distribution of their bud eyes, ensuring that each tuber piece contains one to two bud eyes. These tuber pieces are subsequently sown into the soil. Currently, the preparation of potato tuber pieces relies heavily on manual labor, which presents challenges such as inefficiency and high operational costs. To address these issues, a seed potato cutting machine capable of posture adjustment, cutting, and spraying was designed. Three types of seed potato cutters were developed based on the distribution patterns of bud eyes. The movement mechanism of the posture adjustment process was analyzed, and a mathematical model was established. The key factors influencing the posture adjustment effectiveness were identified through discrete element simulation analysis. Using the qualified rate of potato cutting and the blind eye rate as evaluation metrics, a three-factor, three-level, orthogonal experimental design was implemented. The experimental factors included the rotational speed of the conical roller, the number of conical rollers, and the cutting angle. For the straight-shaped cutter, the optimal combination was determined as follows: a conical roller speed of 12 r/min, 44 conical rollers, and a cutting angle of 0°, yielding a qualified rate of 90.3% and a blind eye rate of 1.86%. For the Y-shaped cutter, the optimal parameters were 14 r/min, 44 conical rollers, and a 5° cutting angle, achieving a qualified rate of 87.9% and a blind eye rate of 2.86%. The cross-shaped cutter performed best at 14 r/min, 44 conical rollers, and a 0° cutting angle, with a qualified rate of 87.1% and a blind eye rate of 3.80%. All optimal configurations met agronomic requirements, demonstrating the efficacy of the designed machine and cutters. Full article

Worldwide, the combination of cutting potato seeds and spoon-type seed-metering devices holds a dominant position in the development of potato planters. In this context, miss-seeding and multi-seeding caused by the seed spoon are important factors contributing to consequent yield reductions. These issues have become key concerns in the precision seeding of potatoes in recent years. This study first analyzes existing research into the monitoring and inhibition of spoon-style potato seed-metering defects. Then, the main problems of spoon-type precision potato planters are analyzed independently in depth, including the lack of monitoring methods, insufficient active inhibition of potato seed-metering defects, unexplored deep additional impacts of the ‘catching-up compensation’ effect, and limited research into multi-picking monitoring and inhibition methods. On this basis, four strategies are proposed: (1) improving the reliability of the seed-metering monitoring system, (2) simplifying the practicality of the miss-picking compensation system, (3) reducing multi-picking based on the trajectory abrupt change in potato seeds, and (4) researching the deep additional effects of the ‘catching-up compensation’ strategy and utilizing multi-means coupling simulation to assist in active seed-metering defect inhibition. Subsequently, relevant conclusions based on the existing literature and in-depth analyses are presented. Finally, prospects for the future development of potato seed-metering technology are discussed. This study aims to provide effective references for achieving intelligent, precise, and information-based potato seed-metering. Full article