Search Results (1,072)

Background/Objectives: Previous research demonstrates higher sodium retention with increasing levels of dietary salt in some populations. Our objective was to determine whole-body sodium retention and sodium distribution on high and low salt diets using rodent models. Methods: Whole body retention of orally dosed Na-22, a gamma emitter, was measured in female growing and adult Sprague-Dawley rats on high (3.1% by wt. of diet) and low salt (0.13% by wt. of diet) diets. In a second study, whole-body sodium retention was compared between destructive inductively coupled plasma optical emission spectroscopy (ICP-OES) and neutron activation analysis (NAA) in adult male and female C57BL/6 mice. Results: Whole body retention of Na-22 was not different due to the age of rats on a high salt diet, but rats fed the high salt diet excreted Na-22 much more rapidly than rats fed a low salt diet. In mice, neither sodium retention nor tissue distribution was affected by dietary salt. Bland–Altman analysis indicated overall agreement between NAA and ICP-OES measurements, with observed systematic positive bias. Conclusions: Dietary salt had little effect on retention in normotensive rodents and should be studied in hypertensive models. Full article

Background/Objectives: Excessive sodium (primarily from sodium chloride, NaCl) intake remains one of the leading dietary risk factors associated with hypertension, cardiovascular disease, and other chronic health conditions worldwide. Commercial diet catering services providing ready-to-eat daily meal plans have become increasingly popular and are often perceived as nutritionally balanced; however, analytical evidence regarding their actual salt content remains limited. The aim of this study was to evaluate the NaCl content of daily food rations (DFRs) offered by commercial diet catering services in Poland. Methods: A total of 120 DFRs representing three dietary patterns (Hashimoto diet, DASH diet, and low-carbohydrate diet) were collected from 40 catering providers. Sodium chloride content was determined using the Mohr titration method. Sodium intake values were estimated by conversion from NaCl equivalents to allow comparison with dietary recommendations. Results: The median NaCl content across all analyzed diets was 14.19 g/day (Q1: 10.62 g; Q3: 17.49 g), corresponding to approximately 284% of the World Health Organization recommended maximum intake of 5 g/day of salt. Nearly half of the analyzed DFRs (45.83%) exceeded the recommended intake by more than threefold. Overall, 99.2% of the analyzed DFRs exceeded recommended NaCl intake levels, while 91.9% did not comply with the values declared by manufacturers. DFRs consisting of five meals contained higher NaCl levels than three-meal plans (p < 0.0196); however, this difference may be related to variation in total food mass rather than meal frequency, as the number of meals was confounded with diet type. Conclusions: These findings suggest that commercially prepared diet catering meals may represent a substantial source of dietary NaCl when used as a primary daily food source. Improved nutritional monitoring, clearer nutrient reporting, and quality control of commercially prepared dietary plans may support public health strategies aimed at reducing NaCl intake. Full article

Background/Objectives: Colorectal cancer (CRC) is a leading cause of cancer-related mortality worldwide, yet the association between folic acid (FA) intake and CRC risk remains controversial. This study investigated the effects of varying dietary FA levels on colorectal carcinogenesis and the underlying mechanisms. Methods: BALB/c mice were fed diets containing FA at <0.1, 2.0, 6.0, 8.0, or 20.0 mg/kg for 14 weeks. After 4 weeks, colorectal tumorigenesis was induced using the azoxymethane/dextran sulfate sodium (AOM/DSS) protocol. Tumor multiplicity, maximum tumor diameter, tumor volume, colorectal length, histopathology, and cell proliferation were assessed. Mechanistic assessments included uracil misincorporation, thymidylate synthase (TS), telomere attrition, genome-wide DNA methylation, RAP1 signaling, immune-related markers, and inflammatory cytokines in colorectal tissues. Results: Both FA deficiency (<0.1 mg/kg) and excess (8.0/20.0 mg/kg) increased colorectal tumor burden, with increased tumor number, larger maximum diameter, greater tumor volume, shortened colorectal length, and enhanced cell proliferation, whereas the 6.0 mg/kg diet group showed the lowest tumor burden. FA deficiency reduced TS expression, elevated deoxyuridine monophosphate (dUMP) levels, decreased deoxythymidine monophosphate (dTMP) levels, increased uracil misincorporation, and exacerbated telomere attrition, as evidenced by shortened telomeres and increased damage. In contrast, excessive FA intake induced Rap1 GTPase-activating protein (RAP1GAP) hypermethylation, reduced Rap1GAP expression, enhanced RAP1 activity, and upregulated programmed death-ligand 1 (PD-L1) and cytotoxic T-lymphocyte-associated protein 4 (CTLA4) expression. Conclusions: Dietary FA can exhibit a U-shaped association with colorectal carcinogenesis, with protective effects observed within an optimal range. FA deficiency and excess may drive tumor development through distinct molecular pathways involving uracil misincorporation-induced telomere attrition and DNA methylation-mediated immunosuppression, respectively. Full article

Background/Objectives: Non-alcoholic fatty liver disease (NAFLD) is a prevalent metabolic disorder for which there are limited pharmacotherapies. Sodium rutin (NaR), a soluble flavonoid derivative, has shown beneficial metabolic effects, but its role in NAFLD remains unclear. This study investigates whether NaR ameliorates high-fat diet (HFD)-induced NAFLD and insulin resistance through promoting hepatic lipophagy. Methods: Male mice aged 8 weeks old were fed a HFD for 12 weeks with/without NaR supplementation. Body weight was measured every week. After 12 weeks of treatment, GTT and ITT were performed to assess insulin resistance. Then, the tissues were collected and hepatic histology, serum biochemistry, and markers of autophagy and senescence were assessed. Results: NaR treatment significantly attenuated HFD-induced weight gain, reduced visceral fat and liver weights, and ameliorated hepatic steatosis and vacuolization. NaR improved serum lipid profiles; lowered alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase levels; and reduced hepatic cellular senescence. NaR enhanced hepatic autophagy, evidenced by decreased p62 levels, increased LC3-II/LC3-I ratio, and enhanced colocalization of lipid droplets with LC3 and LAMP1 in vivo and in vitro. These changes were accompanied by improved glucose tolerance and insulin sensitivity. Conclusions: NaR effectively alleviates HFD-induced NAFLD and insulin resistance by activating hepatic lipophagy. These findings support NaR as a promising multi-targeted therapeutic candidate for NAFLD. Full article

Objective: Vascular function serves as an early indicator of cardiovascular (CV) risk. The intake of n-3 polyunsaturated fatty acids (PUFAs) has been reported to improve arterial properties and reduce CV risk, but evidence in healthy individuals remains limited. This study investigated the effects of consuming n-3 PUFAs-enriched chicken meat on vascular reactivity at both microvascular and macrovascular levels in healthy young adults. Materials and Methods: In this placebo-controlled, double-blind, randomized interventional trial (ClinicalTrials.gov: NCT05725486), 39 participants (aged 20–26 years) were assigned to either the Control group (n = 20; approximately 118 mg n-3 PUFAs/day) or the n-3 PUFA group (n = 19; approximately 1500 mg n-3 PUFAs/day) for three weeks. Microvascular reactivity was assessed via post-occlusive reactive hyperemia (PORH), acetylcholine-induced dilation (AChID), local thermal hyperemia (LTH), and sodium nitroprusside-induced (SNPID) responses. Macrovascular reactivity was measured by brachial artery flow-mediated dilation (FMD) and nitroglycerine-mediated dilation (NTG-MD). Body composition and blood pressure (BP) were recorded before and after the intervention. Results: Both microvascular (PORH, AChID, and LTH) and macrovascular (FMD) endothelium-dependent vasodilation increased in the n-3 PUFAs group following the dietary protocol compared to the Control group. Conversely, the three-week dietary intervention did not influence endothelium-independent dilation in either the microvasculature (SNPID) or macrovasculature (NTG-MD) within the groups compared to baseline, nor were any differences observed between the groups. No significant changes were noted in BP or body composition after either diet. Conclusions: In healthy young adults, consuming the n-3 PUFAs-enriched chicken meat for three weeks improved endothelium-dependent vasodilation in both micro- and macrocirculation, without affecting endothelium-independent responses. These findings suggest that dietary n-3 PUFA intake may provide vascular benefits even in healthy, disease-free individuals at rest. Full article

The search for sustainable alternatives to fishmeal (FM) in aquafeeds represents a major challenge for modern aquaculture. This study evaluated the effects of replacing 35% of FM with defatted Hermetia illucens larvae meal (HIM35) in diets of gilthead seabream (Sparus aurata) reared under full-scale commercial offshore farming conditions. Fillet nutritional quality, fatty acid and amino acid profiles, mineral composition, and microbiological stability during refrigerated storage were assessed. Dietary HIM35 significantly modified the fatty acid profile, increasing saturated fatty acids, particularly lauric and myristic acids, and n-6 polyunsaturated fatty acids. Despite reductions in eicosapentaenoic and docosahexaenoic acids (EPA and DHA), total PUFA and lipid health indices remained within recommended ranges and EPA + DHA levels were above 8%, supporting both fillet nutritional value and fish physiological requirements. Enzymatic indices based on product-to-precursor fatty acid ratios suggested reduced Δ5 + Δ6-desaturase activity. The amino acid profile showed increases in selected essential and non-essential amino acids, while overall protein quality was preserved. HIM35 fillets showed lower sodium and higher zinc contents, whereas increased aluminum levels warrant further investigation. Microbiological analyses confirmed the absence of foodborne pathogens and no effects on spoilage dynamics. Overall, HIM35 represents a safe and effective partial replacement for FM supporting sustainable aquafeed strategies. Full article

Global population growth has intensified the demand for productive and sustainable livestock systems. Lithothamnium calcareum, a calcareous marine alga, has been investigated as a natural feed additive for cattle diets. This study evaluated the effects of L. calcareum supplementation on performance, carcass traits, nutrient digestibility, nitrogen metabolism, urinary and fecal pH, and enteric methane emissions in Nellore heifers during the finishing phase. Thirty-six heifers (BW = 268.8 ± 7.3 kg) were assigned to individual pens in a completely randomized design and fed ad libitum diets (25:75 forage-to-concentrate ratio, DM basis). Treatments were: (1) sodium bicarbonate (110 g/heifer/day) and (2) L. calcareum (60 g/heifer/day). The 96-day trial included 12 days of adaptation and 84 days on the finishing diet. Methane emissions were measured using the sulfur hexafluoride (SF₆) tracer technique. L. calcareum did not affect performance, carcass traits, nitrogen metabolism, or apparent total tract digestibility (all p ≥ 0.106), but reduced urine pH (p ≤ 0.001) and tended to lower methane emissions (−8.2%; p = 0.079). Thus, L. calcareum appears to be a viable natural alternative to sodium bicarbonate in finishing diets for Nellore heifers, maintaining productive performance and potentially reducing enteric methane output. Full article

Background: Systemic inflammation is a key driver of heart failure (HF) progression across all ejection fraction (EF) phenotypes, with diet emerging as a modifiable factor influencing cardiac metabolism and inflammatory signaling. This narrative review integrates current evidence on the inflammatory mechanisms underlying HF, their links with common comorbidities and emerging anti-inflammatory therapeutic strategies, with a particular focus on the role of nutrition in supporting healthy cardiac metabolism. Methods: We searched MEDLINE/PubMed, EMBASE, Web of Science, the Cochrane Library, Scopus and reference lists of relevant publications using terms related to systemic inflammation, dietary patterns and HF prioritizing high-impact studies on nutrition–inflammation–HF interactions published from 2000 onward. Results: Major HF comorbidities sustain chronic, low-grade inflammation through elevated cytokine activity. Dietary patterns—especially those with high Dietary Inflammatory Index (DII)—substantially shape inflammatory milieu. The Mediterranean diet appears to have a favorable inflammatory profile with reduction in circulating pro-inflammatory biomarkers, especially C-reactive protein (CRP) and interleukin-6 (IL-6). Established therapies for HF with reduced ejection fraction and vagus nerve stimulation elicit anti-inflammatory efficacy through cytokine suppression. Sodium glucose cotransporter-2 (SGLT2) inhibitors demonstrate positive metabolic effects and anti-inflammatory actions through decrease in IL-6 and tumor necrosis factor-α (TNF-α). Interleukin-1 blockade has produced heterogeneous clinical outcomes, while definitive findings examining the role of IL-6 inhibitors in inflammation suppression and possible benefit on cardiac outcomes are anticipated. Preliminary data show the potential synergistic effects of dietary patterns/nutrients and pharmacological agents combination on improvement of endothelial function and attenuation of the fibrotic process, although there is a need for further research in large-scale trials. Conclusions: Systemic inflammation demonstrates a key role in HF initiation and progression, and the effect of diet on inflammatory pathways is central. Dietary patterns targeting inflammation-related mechanisms (inflammasome, gut dysbiosis) can lead to attenuation of systemic inflammatory response and restoration of cardiac metabolic flexibility. A deeper mechanistic discernment of cardiac inflammatory cascades, together with identification of HF subpopulations with excessive inflammatory activity, may facilitate the design of targeted randomized controlled trials (RCTs) aiming for novel personalized, inflammation-targeted HF therapies with potential clinical benefit. Full article

This study evaluated the growth performance, carcass characteristics, and meat quality of male Alpine and Saanen goat kids raised under standardized fattening conditions to inform breed-specific strategies for meat production. The study included 36 single-born male kids (18 Alpine and 18 Saanen purebreds) of similar age and live weight. The animals were allocated by breed and randomly assigned to three replicates per breed, with six animals in each replicate. After a two-week adaptation period, the kids were fattened for 12 weeks on an 80:20 concentrate–roughage diet. At the end of the fattening period, all animals were slaughtered, and physical, sensory, and chemical analyses of the meat were performed on the Longissimus lumborum (LL) muscle. Final live weights did not differ significantly between Alpine and Saanen kids, nor did average daily gains. However, the feed conversion ratio favored the Saanen breed, indicating superior feed efficiency. Alpine kids had significantly higher internal fat content, while Saanen kids had a higher proportion of full intestines. Meat pH, color, and texture were similar between breeds. Fatty acid analysis showed that Alpine meat had higher palmitic and palmitoleic acid content, whereas Saanen meat contained more calcium and sodium. These results suggest that both breeds are suitable for high-quality meat production under controlled feeding conditions, but breed-specific differences in fat composition and mineral content may affect nutritional value and market positioning. These findings are valuable for optimizing selection and marketing strategies in goat meat production systems targeting diverse consumer demands. Full article

Background/Objectives: Dietary intake of fruits and vegetables (FVs) has been inversely associated with a lower risk of ulcerative colitis. Using a pig model, we evaluated the effect of FV supplementation on dextran sulfate sodium (DSS)-induced colitis. Methods: Six-week-old pigs were fed a grower diet (negative control), grower diet + 4% DSS (positive control), half-FV diet + DSS, or full-FV diet + DSS. FV levels matched half or full daily recommendations from the Dietary Guidelines for Americans (DGA). Clinical signs were monitored; proximal colon contents (PCs) and mucosa (PCM) were analyzed for metagenome, transcriptome and histopathology. Results: Full-FV pigs showed no diarrhea, less fecal occult blood (FOB), crypt hyperplasia, but no changes in gene expression or microbiome diversity (p < 0.05). Half-FV pigs had increased FOB, differentially expressed genes (DEGs) linked to tissue remodeling, crypt/goblet cell hyperplasia and two cases of diarrhea (p < 0.05). DSS controls showed reduced immune-related DEGs, altered microbiome, PCM erosion, FOB, and persistent diarrhea in one pig (p < 0.05). Conclusions: A three-week full-FV diet conferred protection against DSS-induced colitis, with a dose-dependent protection of intestinal tissue and gut metagenome under inflammatory challenge. Full article

Background: The aim of our study was to analyze the supply of various forms of genistein (nano, micro, and classic) on the content of four macroelements—calcium, magnesium potassium, and sodium—in the kidneys, brains, hearts, livers, spleens and femurs of rats under conditions of mammary gland neoplasia (induced by 7,12-dimethylbenz[a]anthracene (DMBA)). Methods: Thirty-two 30-day-old Sprague-Dawley rats were included in this study. The animals were randomly assigned to four experimental groups: the control group received only a standard diet (without supplementation), while three groups were supplemented with genistein in different forms—nanoparticles (0.1 mg/mL; size 92 ± 41 nm), microparticles (0.1 mg/mL; size 587 ± 83 nm), or macromolecular genistein (0.1 mg/mL). To induce mammary gland cancer, all rats were administered DMBA. Results: In the presented studies, significant changes in the content of elements in the organs of rats supplemented with various forms of genistein were observed. Of particular importance was the occurrence of soft tissue calcifications caused by the dietary supplementation of rats with various forms of genistein, ranging from the classic form to the nanometric form, in the context of an existing mammary gland neoplastic process. Calcium accumulation occurred in various tissues—the brain (from 252% to 449%); the heart (from 159% to 661%); the liver (from 90% to 613%), regardless of the form of genistein; and the spleen (by 127%) and femurs (by 294%) only in the case of nanogenistein supplementation—compared to rats from the control group not supplemented with any form of genistein in conditions of induced mammary gland cancer. Conclusions: Genistein supplementation in cancer conditions affects mineral homeostasis in rats. Full article

Background: Excess body weight before pregnancy is common and may be associated with suboptimal dietary intake and adverse metabolic outcomes. This study aimed to assess dietary intake and selected metabolic parameters in pregnant women with pre-pregnancy overweight or obesity compared with women of normal pre-pregnancy body weight, and to explore changes following nutritional education. Methods: The study included 62 pregnant women between 10 and 36 weeks of gestation. The study group consisted of 44 women with pre-pregnancy overweight or obesity, while the control group included 18 women with normal pre-pregnancy body weight. Dietary intake was assessed using repeated 24 h dietary recalls and analyzed before and after a nutritional education intervention. Nutrient intake was compared with national dietary reference values, and selected biochemical parameters were analyzed. Results: Before nutritional education, diets in both groups were characterized by a high proportion of energy derived from fat, excessive intake of saturated fatty acids (SFAs), sodium, and phosphorus, and insufficient intake of dietary fiber, vitamin D, folates, iron, and iodine. After nutritional education, modest changes in dietary composition were observed, including a reduction in SFA and sucrose intake and a slight shift toward a higher proportion of energy derived from protein. However, improvements in biochemical parameters were limited. Conclusions: In this exploratory study, short-term nutritional education during pregnancy was associated with modest dietary modifications but had a limited impact on metabolic parameters. The results do not support strong clinical recommendations or modifications of existing dietary guidelines but underscore the need for larger, well-powered studies with longer follow-up to better evaluate the effectiveness of nutritional interventions in pregnant women with different pre-pregnancy nutritional statuses. Full article

Background/Objectives: Chronic kidney disease (CKD) is one of the fastest-growing non-communicable diseases globally, with a disproportionate burden in populations undergoing rapid dietary and epidemiological transitions. Beyond traditional clinical risk factors, increasing evidence from nutritional epidemiology suggests that contemporary dietary environments may play a significant role in shaping CKD risk, complications, and progression. This narrative review examines CKD as a potential unintended consequence of global dietary transitions, with particular emphasis on ultra-processed foods and overall diet quality. Methods: A structured narrative review was conducted using evidence from prospective cohort studies, systematic reviews, meta-analyses, and mechanistic research. Findings were synthesized within a population-health framework integrating dietary patterns, food processing classification, and biologically plausible pathways relevant to kidney health. Results: Healthier dietary patterns, including Mediterranean, DASH, and plant-forward diets, are consistently associated with lower risk of incident CKD, slower kidney function decline, and reduced mortality. In contrast, Western dietary patterns characterized by high intake of ultra-processed foods are linked to increased CKD risk and adverse cardiometabolic outcomes. Beyond mediators such as hypertension, diabetes, and obesity, emerging mechanisms include dietary acid load, gut dysbiosis and uremic toxin production, sodium density, and exposure to highly bioavailable phosphate additives. Conclusions: Dietary transitions toward ultra-processed, low-fiber, sodium- and additive-rich food environments may contribute to the growing global burden of CKD through interconnected metabolic, inflammatory, and gut–kidney pathways. Improving diet quality and addressing food processing at the population level represent promising opportunities for CKD prevention and risk reduction. Full article

Background/Objectives: Dietary fibers play key roles in shaping gut microbiome and intestinal homeostasis. While purified diets offer experimental precision and reproducibility in rodent models, they omit the complex mixture of fermentable and non-fermentable fibers found in grain-based chow diets. We hypothesized that excluding fermentable fiber impairs intestinal homeostasis by reducing microbial metabolites and altering the colonic epithelial transcriptome, thereby increasing susceptibility to inflammation. Methods: Wildtype male C57BL/6 mice were maintained on either a standard grain-based chow diet or a purified low-fat diet (LFD) containing 5% non-fermentable cellulose for ten weeks. Fecal microbiomes, short-chain fatty acid (SCFA) profiles, and colonic epithelial transcriptomes were analyzed. A separate group was challenged with dextran sodium sulfate (DSS) following a five-week dietary intervention to compare colitis severity between the two diet groups. Results: Relative to mice fed the grain-based chow, those consuming the purified LFD (containing only non-fermentable cellulose) showed decreased gut microbial diversity and significantly lower SCFA levels. These changes were accompanied by marked differences in colonic epithelial cell transcriptomes. In LFD-fed mice, the top upregulated gene networks included ribosomal pathways and MHC complex protein binding, suggesting increased growth and gut inflammation. The most downregulated pathways included mineral absorption, actin and tubulin binding, and membrane organelle assembly, indicating major alterations in cellular structure and transport. LFD-fed mice also exhibited increased colonic expression of S100a9, a gut inflammation biomarker, and more severe disease symptoms when challenged with DSS compared to chow-fed mice. Conclusions: Fermentable fibers are one of the factors contributing to intestinal homeostasis and mitigating the severity of ulcerative colitis. Full article

Salt reduction is an important strategy for healthy diets. Our previous study developed low-sodium chickpea nang (LCHN) using potassium chloride, wheat gluten, inulin and L-lysine. However, consumers also value taste. The impact of this reformulation on oral processing characteristics remains unclear. This study collected chewing samples from 12 volunteers at five mastication stages (0%, 25%, 50%, 75%, and 100%) of regular chickpea nang (CHN) and LCHN, measuring chewing parameters, bolus moisture content, saliva addition amount, and flow rate. Results indicated that LCHN had a significantly shorter swallowing time (24.22 ± 3.63 s vs. 27.84 ± 6.01 s, p < 0.05, Cohen’s d = 0.73), while the number of chews (Nc), chewing frequency (Fc), bolus moisture content, and saliva flow rate showed no inter-group differences across all mastication stages (p > 0.05). Bolus moisture content increased significantly with mastication progression in both groups (p < 0.05), whereas saliva addition amount and flow rate decreased significantly (p < 0.05). Additionally, higher chewing frequency correlated with increased saliva addition amount and reduced flow rate (p < 0.05). In CHN, the Nc positively correlated with chewing time (r = 0.452, p < 0.01) and frequency (r = 0.458, p < 0.01), whereas in LCHN it negatively correlated with time (r = −0.329, p < 0.05) and positively with frequency (r = 0.884, p < 0.01). These findings provide theoretical basis for low-sodium baked product development. Full article