Search Results (1,504)

High temperature stress is a critical factor affecting the growth and yield of melons (Cucumis melo L.), and improving heat tolerance is therefore crucial for stable production. While the overexpression of the CmDUF239-1 gene is known to improve salt tolerance in melons, its impact on heat tolerance remains unexplored. The role of the CmDUF239-1 gene in enhancing heat tolerance and its underlying mechanisms was investigated in this study. Melon seedlings overexpressing CmDUF239-1 (OEDUF239-1), generated via root transformation, exhibited significantly lower reductions in fresh and dry mass under heat stress compared to controls, indicating enhanced heat tolerance. One day post-stress, antioxidant enzyme activities (SOD, POD, CAT, APX, and GR) increased significantly in OEDUF239-1, while malondialdehyde (MDA) levels decreased. Additionally, proline content and the activity of its synthesizing enzyme (P5CS) rose, whereas the activity of proline dehydrogenase (ProDH) dropped. Transcriptomic and qPCR analyses revealed that CmDUF239-1 overexpression upregulated antioxidant enzyme-related genes (e.g., CmCSD1, CmPOD1) and proline-related genes (e.g., CmP5CS), as well as Heat Shock Protein (HSP) genes (e.g., CmHSP17.6II, CmHSP18.2). In summary, the enhancement of heat tolerance in melon by the CmDUF239-1 gene was mediated through the upregulation of genes involved in antioxidant defense and proline metabolism, together with increased accumulation of HSPs, providing a mechanistic basis for heat-resilient breeding programs. Full article

In the context of contemporary climate change, drought is widely recognized as a major stressor affecting plant growth. While numerous studies have demonstrated that Serendipita indica enhances stress resistance in host plants and is widely used in agriculture, research on its symbiotic interactions with woody plants for improving drought tolerance remains limited. This study investigated the effects of S. indica inoculation on the growth of Phoebe sheareri seedlings under varying drought conditions—well-watered (WW), moderate drought (MD), and severe drought (SD)—and explored the physiological mechanisms underlying improved drought resistance. The results showed that under WW conditions, S. indica inoculation promoted seedling growth and development. Under MD and SD conditions, although drought stress inhibited growth, inoculation significantly increased plant biomass, root parameters, chlorophyll content, and photosynthetic efficiency. Additionally, it alleviated drought-induced damage by reducing REC, MDA, H₂O₂, and O₂⁻ levels, while enhancing SOD, POD, and CAT activities, and increasing root ABA, GA, IAA, and CTK content. Under MD stress, adaptive changes in root architecture and hormone levels were observed, including increases in total root length, surface area, volume, average diameter, and elevated IAA and CTK levels—all of which were further enhanced by S. indica inoculation. In conclusion, symbiosis with S. indica improved drought tolerance in P. sheareri seedlings likely through enhanced photosynthesis, antioxidant enzyme activity, and hormone regulation. Full article

Background: Investigating human exposure to toxic contaminants through dietary consumption is critical to identify disease risk factors and health guidelines. Methods: In this study, we developed a cross-sectional online survey to collect information about dietary patterns and related food consumption habits among adults (age ≥ 18) and adolescents (ages 13–17) in Southern California, focusing on popular staple foods and/or those targeted most commonly under California’s Proposition 65 law for lead and acrylamide exposure. Results: Results identified root vegetables, rice, leafy greens, pasta/noodles, tea, juice, and seafood to be among the most heavily consumed foods by mass, while the daily intake of many foods such as stuffed grape leaves, tamarind/chili candy and herbs/spices varied by age and race/ethnicity, suggesting that many of Proposition 65’s pollution allowances may be exacerbating issues of health inequity and environmental injustice. Moreover, findings from this study indicate that the methods of exposure assessment often applied under Prop 65, especially relating to herbs/spices, are likely to underestimate single-day exposures, thus allowing unsafe products on the market without warning labels. Conclusions: Study outcomes are broadly relevant to environmental health and nutrition science, with particular relevance to public health practitioners and California’s Prop 65 regulators and other stakeholders. Full article

Essential oils play important roles in the modern food industry as additives and spices. At the same time, most essential oils have broad-spectrum bacteriostatic properties and can be used as natural antimicrobial materials. However, the application of essential oils is limited due to their strong volatility and insolubility in aqueous substrates. In this study, we used ultrasonic emulsification, carboxymethyl chitosan, and Tween 80 to formulate tea tree essential oil (TTO) nanoemulsions with high stability. With a minimum diameter of about 51 nm (PDI = 0.236 ± 0.021) post-emulsification, the TTO nanoemulsions disperse effectively in the drainage system and exhibit good stability after 14 days of storage. In addition, the bioactivity (antibacterial and antioxidant) of TTO nanoemulsions was significantly enhanced following emulsification, as evidenced by MIC and DPPH assays, indicating that nano-emulsification is beneficial to the development of various essential oils. TTO nanoemulsions can be used as a new food preservative to control the growth of bacteria and prevent the deterioration of food via oxidation. Full article

This paper presents two classic analog oscillators: a relaxation oscillator and a Wien bridge one, where a memristor replaces a resistor. The circuits are simulated in TopSPICE 7.12 using a memristor emulation circuit and commercially available components to evaluate the memristor’s impact. In the case of the relaxation oscillator, which includes the memristor, a notable increase in oscillation frequency was observed compared to the classical circuit, with a nearly 10-fold increase from 790 Hz to 7.78 kHz while maintaining a constant amplitude. This confirms the influence of the memristor’s dynamic resistance on the circuit time constant. On the other hand, the Wien-bridge oscillator exhibits variations in specific parameters, such as peak voltage, amplitude, and frequency. In this case, the oscillation frequency decreased from 405 Hz to 146 Hz with the addition of the memristor, a characteristic introduced by the proposed memristive element’s nonlinear interactions. Experimental results confirm the feasibility of incorporating memristors into classical oscillator circuits, enabling frequency changes while maintaining stable oscillations, allowing reconfigurable and adaptable analog designs that leverage the properties of memristive devices. Full article

The dried herb of blue fenugreek is used as a spice in the alpine region for the preparation of traditional bread and cheese. After drying, the herb is stored for a period of six to twelve months. During this time, the herb is expected to undergo changes in the compositions of the major flavor- and odor-determining compounds. To identify eventual biochemical processes, we applied different growing (conventional and sterile) and drying (air- and freeze drying) conditions and subsequently conducted periodical analysis of key aroma constituents (α-keto acids and volatile compounds) by LC-MS and GC-MS. The amount of glyoxylic acid was drastically increased in the air-dried sample, while the freeze-dried sample showed significantly higher amounts of α-keto-glutaric acid and pyruvic acid, respectively. During storage, a decrease in sulfuric compounds and an increase in alkane aldehydes were observed when comparing conventional and sterile samples. However, this increase was even greater for monoterpenes (especially camphor and p-cymene), showing thrice as high amounts after storage. Interestingly, both compounds were only formed significantly during the storage under conventional conditions, indicating that their production is induced/caused by microbial organisms. Full article

Broussonetia papyrifera has strong adaptability and exhibits a strong ability to accumulate selenium. Its leaves are rich in crude protein, amino acids, and minerals, making them high-quality feed materials. To improve the selenium-enriched ability of B. papyrifera and promote the development of selenium-enriched agricultural products, we screened and identified the sulfate transporters associated with selenium absorption in B. papyrifera. By treating the leaves of B. papyrifera with different concentrations of sodium selenate and analyzing the correlation between gene expression and selenium content, we identified BpSULTR3;1 and BpSULTR3;4, which may be involved in selenium absorption and transport in B. papyrifera. We further validated the functions of BpSULTR3;1 and BpSULTR3;4 through transgenic experiments in Arabidopsis thaliana. The results showed that overexpressing BpSULTR3;1 significantly increased the total selenium content in A. thaliana, up to 2.31 times, and also increased the contents of three forms of organic selenium (SeCys₂, MeSeCys and SeMet) in transgenic A. thaliana. These findings provide solid theoretical support for improving B. papyrifera’s selenium enrichment ability through genetic improvement. Full article

Background /Objectives: The rising prevalence of metabolic disorders, such as obesity, is linked to increased consumption of high-calorie foods and sedentary lifestyles. While conventional treatments rely on lifestyle modifications and pharmaceuticals, these often have limitations and adverse effects. As an alternative, natural compounds like β-caryophyllene (BCP), found in spices such as black pepper and cloves, have gained interest due to their anti-inflammatory and metabolic properties. This study investigated the effects of BCP on the gut–brain axis in obese C57BL/6J mice. Methods: Quantitative real-time PCR (RT-qPCR) was performed using a Rotor-GeneQ thermocycler (Qiagen). Relative gene expression levels were normalized to the reference gene’s transcript levels (2^−∆∆Ct method). Results: BCP was found to modulate key receptors, including FFAR3, LEPR, and GHSR, which are involved in appetite regulation and insulin sensitivity. Its action on the CNR2 (CB2 receptor) suggests additional benefits in energy balance and anorexigenic activity. Conclusions: These findings support BCP’s potential as a complementary therapy for obesity, though further studies are needed to confirm its efficacy in humans. Its safety profile and multifactorial effects make it a promising alternative to conventional treatments. Full article

Single Event Transients (SETs) in clock-distribution networks are a major source of soft errors in synchronous systems. We present a practical framework that assesses SET risk early in the design cycle, before layout and parasitics, using a Vulnerability Function (VF) derived from Verilog fault injection. This framework guides targeted Engineering Change Orders (ECOs), such as clock-net remapping, re-routing, and the selective insertion of SET filters, within a reproducible open-source flow (Yosys, OpenROAD, OpenSTA). A new analytical Soft Error Rate (SER) model for clock trees is also proposed, which decomposes contributions from the root, intermediate levels, and leaves, and is calibrated by SPICE-measured propagation probabilities, area, and particle flux. When coupled with throughput, this model yields a frequency-aware system-level Bit Error Rate (${BER}_{sys}$). The methodology was validated on a First-In First-Out (FIFO) memory, demonstrating a significant vulnerability reduction of approximately 3.35× in READ mode and 2.67× in WRITE mode. Frequency sweeps show monotonic decreases in both clock-tree vulnerability and ${BER}_{sys}$ at higher clock frequencies, a trend attributed to temporal masking and throughput effects. Cross-node SPICE characterization between 65 nm and 28 nm reveals a technology-dependent effect: for the same injected charge, the 28 nm process produces a shorter root-level pulse, which lowers the propagation probability relative to 65 nm and shifts the optimal clock-tree partition. These findings underscore the framework’s key innovations: a technology-independent, early-stage VF for ranking critical clock nets; a clock-tree SER model calibrated by measured propagation probabilities; an ECO loop that converts VF insights into concrete hardening actions; and a fully reproducible open-source implementation. The paper’s scope is architectural and pre-layout, with extensions to broader circuit classes and a full electrical analysis outlined for future work. Full article

We present an automated and fully reproducible pipeline for restoring motion-smeared Mars Express SRC images of Phobos. A one-dimensional motion point spread function (PSF) is derived directly from SPICE geometry and microsecond-precision exposure timing, and Wiener deconvolution (SNR = 16 dB) is applied to recover image sharpness. Tested on 14 images from 4 orbits spanning slant distances of 52–292 km, exposures of 14–20 milliseconds, sampling of 0.47–2.7 m/pixel, and PSF lengths of 11–119 pixels, the method achieves up to 31.7 dB PSNR, 0.78 SSIM, and positive sharpness gains across all cases. The restored images reveal sub-meter surface features previously obscured by motion blur, with residual energy reduced relative to the acquisition model. The workflow relies solely on open data and open-source tools (ISIS, ALE/SpiceyPy, OpenCV), requires no star-field calibration, and generalizes to other motion-degraded planetary datasets, providing a fully transparent and reproducible solution for high-resolution planetary imaging. Full article

Background: Synthetic cannabinoid receptor agonists (SCRAs, commercially known as “Spice”) have become a leading cause of substance-induced psychosis worldwide. These compounds show strong associations not only with acute psychotic episodes but also, in a subset of patients, with persistent or relapsing psychotic disorders, patterns that raise concern about progression to schizophrenia. Yet clinicians still lack clear, evidence-based guidance, and the optimal management of SCRA-induced psychosis remains inadequately defined. Methods: We carried out a systematic search of PubMed, Scopus, and Web of Science on 2 April 2025, identifying 35 primary studies that together describe roughly 4600 clinical presentations (≈77% male; mean age: 24.7 years). Results: Across diverse settings a convergent three-step pharmacological strategy emerged. First, rapid tranquillization with parenteral benzodiazepines consistently controlled severe agitation and autonomic instability. Second, when florid psychosis persisted beyond 30–60 min, clinicians introduced a second-generation antipsychotic—most commonly olanzapine, risperidone, or aripiprazole—often at doses exceeding those used for primary psychoses. Third, for the minority of refractory or relapse-prone cases, escalation to long-acting injectable formulations or low-dose clozapine achieved symptom control, even at plasma levels below those required in treatment-resistant schizophrenia. Although the evidence base consists largely of uncontrolled clinical descriptions, across studies, a recurrent clinical pattern was observed: initial benzodiazepines for agitation, followed by antipsychotics when psychosis persisted and escalation to clozapine or long-acting injectables in refractory cases. This approach appears to be associated with symptom improvement, although the certainty of the evidence is low to very low. Conclusions. Prospective, comparative studies are urgently needed to refine dosing, directly compare antipsychotic classes, and evaluate emerging cannabinoid-modulating interventions. Full article

Background: Holistic care in the Intensive Care Unit (ICU) addresses the full spectrum of patient needs—physical, emotional, psychological, social, spiritual, and environmental—to support recovery and improve long-term outcomes after critical illness. Objective: This systematic review aimed to synthesize evidence on the effectiveness of holistic care interventions across these six dimensions of wellness in adult ICU patients. Methods: A systematic search of PubMed, Scopus, and Web of Science was conducted following PRISMA guidelines. The SPICE framework was used to define the scope (Setting: ICU; Perspective: patients; Intervention: holistic care; Comparison: standard care; Evaluation: multi-dimensional outcomes). Studies published in English between 1999 and 2024 were included. Methodological quality was appraised using Joanna Briggs Institute (JBI) tools. Results: Seven studies, comprising randomized controlled trials, observational, and mixed-methods designs, met the inclusion criteria. The interventions were diverse, encompassing corporeal rehabilitation, spiritual care toolkits, reflexology, early physical therapy, patient diaries, and family involvement. A narrative synthesis of these heterogeneous studies suggested potential benefits and high acceptability for various patient-centered outcomes. Conclusions: The limited but promising evidence indicates that holistic care interventions may contribute positively to ICU patient recovery. The findings underscore the need for more robust, high-quality research to conclusively determine their efficacy and support their integration into standard critical care practice. Full article

Powdered foods are matrices transformed into fine, loose solid particles through dehydration and/or milling, which enhances stability, storage, and transport. Due to their high commercial value and susceptibility to fraudulent practices, detecting adulterants in powdered foods is essential for ensuring food safety and protecting consumer health and the economy. Food fraud in powdered products, such as spices, cereals, dairy-based powders, and dietary supplements, poses an increasing risk to public health and consumer trust. These products were selected as representative matrices due to their high nutritional and economic relevance, which also makes them more susceptible to adulteration and hidden potential health risks from hidden contaminants. Recent studies highlight the potential of spectroscopic techniques combined with chemometrics as rapid, non-destructive, and cost-effective tools for authentication. This narrative review synthesizes recent literature (2020–2025) on the application of near-infrared (NIR) spectroscopy combined with chemometric techniques for adulterant detection in powdered foods. Advances in spectral preprocessing, variable selection, classification, and regression models are discussed alongside the most common adulterants and their nutritional and toxicological implications. Furthermore, the applicability of portable versus benchtop NIR devices is compared. The main contribution of this review lies in critically analyzing methodological frameworks, mapping current gaps, and identifying emerging trends, such as digital integration, self-adaptive chemometric models, and real-time on-site authentication, positioning NIR spectroscopy as a promising tool for food authentication and quality control. Full article

Saffron (Crocus sativus L.), a perennial plant of the Iridaceae family, which is also known as “red gold”, is one of the most expensive spices throughout the world. Originally, it was mainly used as a condiment and natural dye for food, and as a medicinal plant in folk medicine. Its cultivation is characterized by an extensive use of labor, since most of the crop management techniques (e.g., sowing, weeding, flower picking, and stigma separation) are performed manually. The aim of this work is to investigate how the adoption of sustainable cultivation techniques could improve the profitability of saffron at the primary level. Thus, economic and technical data were collected directly on a farm in a marginal area in Northwestern Italy, in order to compare the productivity and profitability of traditional and innovative cultivation techniques. The effect of sustainable practices, such as the use of beneficial microorganisms, that is, arbuscular mycorrhizal fungi (AMF), on the productivity of saffron was considered. In a previous work, AMF inoculation with Rhizophagus intraradices and Funneliformis mosseae led to an increase in the flower and saffron spice yields, compared to uninoculated controls. The profitability of the saffron (including tepals, its by-product) considered in our case study, expressed as economic profit (pure profit), was found to be slightly negative for the traditional cultivation method (without the use of AMF) and also, albeit to a lesser extent, for the innovative technology (with the use of AMF). This slightly negative result is mainly due to the implicit cost of family labor for both the traditional and innovative cultivation techniques. The results of our study can be considered a further step in favor of the use of cultivation techniques that improve crop productivity and, at the same time, are sustainable. They also support the spread of minor crops, which, nevertheless, are important to maintain agricultural activities in marginal territories. Full article

Background and Clinical Significance: The recreational use of semisynthetic cannabinoids (SSCs) is increasing, and SSCs account for more than 40% of all new substances reported at the European level. Although designed to mimic the effects of tetrahydrocannabinol (THC), the primary psychoactive compound in cannabis, evidence suggests that certain SSCs may elicit stronger, prolonged and unintended pharmacological effects. SSCs are easily accessible, particularly via online retailers, but in some countries, SSCs are also sold in convenience stores or specialty stores selling legal low-THC or cannabidiol (CBD) products. Often, SSCs are sold as “legal highs” and are found in various forms, including herbal mixtures (spice), vape products, and edibles such as cookies and candies, specifically targeting young users, including children. The products are frequently mislabeled and sold as souvenirs or aromatic potpourri to bypass regulations. Case Presentation: We present a case of a male in his early forties who was admitted to the Emergency Department due to noticeable deficits in alertness and responsiveness after recreational ingestion of two cannabis cookies labeled to contain 40 mg “CC9” and a bite of a gummy with unknown contents. The patient experienced vomiting and visual problems, and suffered from nine days of cognitive and physical impairment. HHC-C9, a novel SSC, was detected in blood through forensic toxicological analysis. Conclusions: Recreational use of HHC-C9 can cause vomiting, visual disturbances, and drowsiness, potentially requiring hospital treatment. Potency, clinical effects, and toxicity of SSCs can vary significantly, and in combination with easy accessibility, SSCs pose a potential risk of intoxication to unaware consumers. Full article