Search Results (2,777)

Acetamiprid (ACE) and thiacloprid (THIA) are the dominant cyano-substituted neonicotinoids detected in fruit juices and bottled water, which raises food-safety concerns and regulatory scrutiny. Conventional purification with activated carbon or advanced oxidation shows limited selectivity and has a high energy demand. Covalent organic frameworks (COFs) offer tunable chemistry for targeted adsorption, yet no strategy exists to engineer COF sites that preferentially recognize the cyano group of ACE/THIA. Here, we synthesized a magnetic core-shell adsorbent, Fe₃O₄@COF(TBTD-BD)-Au, by growing cyano-affinitive Au nanoparticles on a Cl-decorated COF shell surrounding a Fe₃O₄ core. Under optimized conditions (pH 6.0, 25 °C), the Fe₃O₄@COF(TBTD-BD)-Au achieved maximum adsorption capacities of 157 mg g⁻¹ (ACE) and 156 mg g⁻¹ (THIA). Uptake followed pseudo-second-order kinetics and the Freundlich isotherm; thermodynamic analysis confirmed an endothermic, spontaneous process. Competitive tests showed >80% removal of ACE and THIA in the presence of four co-occurring neonicotinoids, and the adsorbent retained 91.5% of its initial capacity after six adsorption–desorption cycles. Synergistic Au-cyano coordination, Cl-mediated hydrogen bonding, and π–π stacking confinement confer high selectivity and capacity. This ligand-guided, post-functionalized COF provides promising potential in the field of food sample treatment for contaminant removal. Full article

This research seeks to investigate extremely efficient catalysts for the nitrogen reduction process (NRR), utilizing machine learning (ML)-aided density functional theory (DFT) computations. Specifically, we investigate dual transition metal atoms anchored on hexagonal nitrogen-doped graphene (TM₁-TM₂@N₆G) as prospective high-activity catalysts for the NRR. The findings indicate that the synergistic effect of dual transition metal atoms in the TM₁-TM₂@N₆G catalyst overcomes the intrinsic constraints of the linear relationship among intermediates, facilitating the activation and adsorption of N₂, thereby exhibiting significant potential for ammonia synthesis through N₂ reduction. Particularly, four catalysts screened by ML and DFT exhibit good stability and excellent selectivity and activation towards N₂. Among them, the catalysts Ti-Cr@N₆G, Ti-Mo@N₆G, and Ti-Pd@N₆G possess two reaction pathways with minimum reaction energies of 0.55 eV, 0.50 eV, and 0.40 eV, respectively. Remarkably, Ti-Co@N₆G, which features a single reaction pathway, exhibits a reaction energy lower than 0.05 eV, allowing the NRR to proceed spontaneously. It is noteworthy that incorporating ML into DFT calculations facilitates the rapid screening of all transition metal combinations, significantly accelerating the research on catalytic performance and optimizing the selection of catalysts. Full article

A series of silver-polygalacturonate complexes with improved structure and activity against bacterial infections was developed. Pure sodium polygalacturonate was obtained by saponification of a pectin precursor and identified by NMR as predominantly homogalacturonan (uronide content 95%). Polygalacturonate complexes with ionic and borohydride-reduced silver with a controllable metallic component were synthesized; the role of spontaneous Ag⁺ reduction was revealed. The presence of uniform 5 nm nanoparticles and negligible particulate by-products in the reduced complexes was verified. The complexes showed similar silver-normalized activity against non-resistant bacteria, irrespective of complex stoichiometry/silver state. Pharmaceutical silver proteinate with a similar nanoparticle profile exhibited the same silver-normalized activity, indicating the lack of a ligand effect. The Ag⁺ complex was more effective against some hospital drug-resistant strains. The cytotoxicity of the complexes depended on fibroblast type, silver state, ligand type, exposure time, presumably in association with cellular availability and glutathione depletion. The complexes were administered to rats with excisional wounds persistently infected with S. aureus. Swab/histological analyses of the treated wounds revealed decreased bacterial burden/tissue damage, along with promotion of wound contraction/closure and matrix formation. The nanoparticle complexes that were compared had similar antibacterial/regenerative effects, while the Ag⁺ complex demonstrated higher efficacy in vivo. These results encourage the use of the developed silver-polygalacturonate complexes as antibacterial substances. Full article

Background/Objectives: General movement assessment (GMA) is a clinical assessment tool used to predict risk for cerebral palsy (CP) in young infants. Equal access is challenging since GMA-trained personnel is a limited resource. An implementation study aimed to offer all high-risk infants born in the Central Norway Regional Health Authority equal access to GMA as part of the standard follow-up. This study explored the local health care personnel (HCP) experiences with early risk assessment for CP in young infants using remote GMA. Methods: This was a qualitative study with one focus group and four individual interviews. Participants were HCP from the local follow-up clinics who had experience with GMA. Analyses were inspired by Malterud’s systematic text condensation. Results: Attitudes towards GMA were, in general, positive, and GMA was considered an important and gentle examination contributing to earlier initiation of correct follow-up actions and appropriate treatment. The GMA results could improve communication between HCP and parents, and lead to a closer local municipality follow-up if GMA result was abnormal. Parents were given an active role with home video recording, which was considered family empowering. Especially pediatricians wanted more detailed information about the qualities of spontaneous movements to support clinical decision-making. Conclusions: This study indicated that further implementation of the GMA method to assess the risk of CP in high-risk infants could be recommended, and that GMA was a gentle method for the purpose. As suggested by pediatricians in this study, more detailed assessments using the GMA beyond FMs could be further explored as support to clinical decisions. The insight from this study may inform implementation in similar contexts. Full article

Chickpea protein hydrolysates have antihypertensive potential. However, neither the effect of their daily consumption on blood pressure (BP) nor their potential antihypertensive mechanisms has been evaluated. Thus, both the antihypertensive effect of an optimized chickpea protein hydrolysate (OCPH) and its potential mechanisms were assessed in spontaneously hypertensive rats. OCPH (50 mg/kg of body weight) was supplemented daily (5 weeks), BP levels were measured, and mRNA relative levels (angiotensin-converting enzyme-I (ACE1), renin, AT₁R receptor, ACE2 and Mas1) in the kidneys were determined. BP (systolic, diastolic, and mean) levels were lowered after five days of OCPH supplementation (p < 0.05 vs. control group) and the hypotensive effect was up to −39.80 mmHg (p < 0.05). Furthermore, the supplementation increased ACE2 (67.30%) and Mas1 (61.1%) mRNA levels (p < 0.05 vs. control group). ACE1, renin and AT₁R receptor mRNA levels were similar between groups (p > 0.05). A negative correlation of ACE2 mRNA levels with BP was found (p < 0.05). The findings support that OCPH activates the ACE2/Ang-(1–7)/Mas1 pathway of the renin–angiotensin–aldosterone system, maintaining a reduction in BP after daily supplementation. Further studies to evaluate the potential of the OCPH for functional food and nutraceutical development are justified. Full article

As a common substitute for brominated flame retardants (BFRs), organophosphate flame retardants (OPFRs) have been insufficiently studied in terms of their ecotoxicological impacts on plastic biodegradation processes in invertebrate systems. This study investigated the impact of an OPFR, tris (1-chloro-2-propyl) phosphate (TCPP), on the dietary behavior and gut microbiota of Galleria mellonella (Linnaeus, 1758) (Lepidoptera: Pyralidae) larvae during the biodegradation of rigid polyurethane (RPU), as well as the fate of TCPP. The results show that TCPP interfered with larval feeding activity, hindered the nutritional conversion of food, and triggered metabolic compensation through lipid reserve catabolism. Notably, mass balance analysis revealed that bioaccumulation of TCPP was negligible, with most of it excreted through frass, indicating limited biodegradation of this organophosphate ester. 16S rRNA sequencing indicated that TCPP drove the reconstruction of gut microbiota in larvae and identified three dominant bacteria of Morganellaceae, Enterobacteriaccae, and Staphylococcaceae families, as well as non-dominant bacteria of Klebsiella and Vagococcaceae families, as characteristic microbiota contributing to RPU and TCPP biotransformation. This study serves as a reminder to pay attention to the toxicity, migration, and transformation of OPFRs in biodegradable plastics. Notably, TCPP, a dominant chlorinated OPFR, exhibits environmental persistence with limited biodegradability and low bioaccumulation, traits which hinder the spontaneous attenuation of plastic waste in ecosystems and undermine the sustainability of the plastic lifecycle. This work emphasizes the need to integrate risk assessments of specific additives into the plastic waste management framework and to develop targeted detoxification strategies for promoting a sustainable material lifecycle. Full article

Background/Objectives: In-hospital cardiac arrest (IHCA) remains a critical event with high mortality, requiring coordinated multidisciplinary response. Return of spontaneous circulation (ROSC) and hospital discharge rates are key quality indicators in resuscitation efforts. In Costa Rica, there is limited published data on team performance, protocol adherence, and the pharmacist’s role in code blue events, despite similar evidence gaps across Latin America. This study aimed to evaluate clinical outcomes and operational performance of in-hospital cardiac arrest events at a Costa Rica hospital. Methods: This retrospective cohort study included 77 adult patients who experienced IHCA at Clínica Bíblica between 2020 and 2024. Data collection was conducted between February and May 2025 from electronic medical records and code blue activation logs. Clinical variables, comorbidities, pharmacologic interventions, and outcomes were analyzed. Predictive models (Charlson Comorbidity Index [CCI], IHCA-ROSC, RISQ-PATH) and Kaplan–Meier survival analysis were applied. Results: ROSC was achieved in 55.8% of patients, and 21% were discharged alive. Asystole was the predominant initial rhythm (76.6%), and comorbidities such as renal disease and myocardial infarction were most frequent. A higher comorbidity burden was significantly associated with lower discharge rates (p = 0.032). Despite 98.7% of patients being classified as low probability for ROSC by the IHCA-ROSC model, observed outcomes exceeded expectations (predicted: 5.53% vs. actual: 55.84%; p < 0.000001). The code team adhered to institutional protocols in 100% of cases, with clinical pharmacists playing a key role in documentation and medication tracking. Conclusions: Structured multidisciplinary response was associated with ROSC rates notably higher than predicted by validated models. Opportunities for improvement include post-event laboratory testing, pharmacist-led documentation, and therapeutic hypothermia in shockable rhythms. Full article

Human papillomavirus (HPV) represents the most common sexually transmitted infection worldwide and a major public health challenge. Nearly all sexually active individuals will acquire HPV during their lifetime, with the highest prevalence observed in adolescents and young adults shortly after sexual debut. More than 200 genotypes have been described, ranging from low-risk types, mainly responsible for benign lesions, to high-risk types, which are associated with cervical, anogenital, and head and neck cancers. While most infections are transient and spontaneously cleared by the immune system, persistent high-risk HPV can lead to precancerous lesions and malignant transformation, often in synergy with other sexually transmitted pathogens or in the context of microbiome imbalance. The introduction of vaccines and advanced screening technologies has substantially modified prevention strategies. Vaccination coverage remains heterogeneous, with persistent gaps particularly among males due to cultural, social, and educational barriers. Schools are increasingly recognized as strategic environments to promote awareness, sex education, and gender-neutral vaccination. Innovative approaches such as microbiome modulation, therapeutic vaccines, and liquid biopsy biomarkers are emerging as promising perspectives. This review aims to provide an updated overview of HPV epidemiology, clinical impact, prevention strategies, and future frontiers, with special attention to adolescents as a priority target group. Full article

This study introduces a novel photocatalyst derived from pumpkin peel bio-waste, calcined at 200 °C and incorporated with magnetite nanoparticles to form a hybrid PK-P/Fe₃O₄ catalyst. The material was characterized using X-ray diffraction (XRD), diffuse reflectance spectra (DRS), and scanning electron microscopy (SEM) with energy-dispersive X-ray spectroscopy (EDX) mapping to confirm its structure and elemental distribution. The catalyst was applied for the photo-Fenton degradation of Synozol Red KHL dye under natural solution conditions (pH 5.7). Optimal parameters were achieved with a 20 mg/L catalyst and 200 mg/L H₂O₂, resulting in complete dye removal within 25 min of irradiation. The PK-P/Fe₃O₄ catalyst exhibited excellent reusability, retaining 72% removal efficiency after 10 successive cycles. Kinetic analysis confirmed a first-order model, while thermodynamic evaluation revealed a non-spontaneous, endothermic process with a low activation energy barrier, indicating energy-efficient dye degradation. These findings highlight the potential of bio-waste-derived PK-P/Fe₃O₄ as a sustainable, low-cost, and highly effective catalyst for treating dye-polluted wastewater under photo-Fenton conditions. Full article

In their natural habitat, male Betta splendens are territorial resource defenders, whereas females are non-territorial opportunistic foragers. This ecological difference suggests that males may be more capable of delaying gratification for food rewards. The present study examined impulsive choice in Betta splendens through two experiments comparing subjects’ choices between a Smaller-Sooner (SS) reward (1 pellet immediately) and a Larger-Later (LL) reward (3 pellets after 15 s). In Experiment I, the choice distributions of males were more likely to stabilize on the LL option over the SS option, whereas females’ choice distributions were equally likely to stabilize on either option. These findings indicate that most males demonstrated spontaneous behavioral self-control without specialized training, while females were collectively indifferent. Experiment II investigated whether dopamine modulates this behavior by administering oral L-Dopa (60 mg/kg) to males before trials. Using the same procedures, only 30% of L-Dopa-treated males’ choice distributions stabilized on the LL reward, while 70% of experimental males’ choice distributions stabilized on the SS option; the choice distributions of control males were equally likely to stabilize on either reward. These results suggest that elevated dopaminergic activity increases impulsive choice in male Betta splendens. Future studies should examine dopamine agonists and antagonists, as well as female responses, to further clarify dopamine’s role in reward valuation and behavioral self-control in Betta splendens. Full article

Corbicula fluminea protein (CFP) and cyanidin-3-O-glucoside (C3G) are natural nutrient fortifiers. During consumption or processing, they may interact with each other, inducing alternations in their structural and functional properties. However, nothing was known about the mechanism of their interaction and their synergistic antioxidant effect. In this research, C3G was physically mixed with CFP to simulate practical scenarios. The impact of the presence of C3G on the multispectral characteristics, antioxidant activity, and particle properties of CFP was examined and compared to chemically fabricated C3G-CFP covalent conjugates. The results indicate that C3G tended to spontaneously bind to CFP and formed compact non-covalent complex, with hydrophobic forces predominantly governing the interaction. This binding resulted in the statically quenched intrinsic fluorescence of CFP, accompanied by a dynamic model. Moreover, C3G preferentially induced Trp residue in CFP exposed to a more polar microenvironment, yet it exerted nearly no effects on CFP when analyzed using ultraviolet–visible (UV-Vis) spectroscopy and synchronous fluorescence spectroscopy (SFS). Additionally, although the formed non-covalent complex demonstrated strengthened antioxidant capacity, C3G displayed an antagonistic effect with CFP, whereas lower C3G concentrations led to synergistic effects in covalent conjugates. These findings provide new insights into the effective application of C3G and CFP as nutritional antioxidants. Full article

Periodontitis is a chronic oral inflammatory disease whose treatment is often hindered by poor drug retention, prolonged therapeutic regimens, and the rise of antibiotic resistance. In this study, we developed a Hydrogel@ZIF-8@metronidazole (Hydrogel@ZIF-8@MNZ) nanocomposite dressing for targeted, sustained, and in situ antimicrobial therapy. This system integrates ZIF-8, a pH-responsive metal–organic framework, with the antimicrobial agent metronidazole (MNZ), encapsulated within a crosslinked hydrogel matrix to enhance stability and retention in the oral environment. Drug release studies demonstrated that MNZ release was significantly accelerated under acidic conditions (pH 5.0), mimicking the periodontal microenvironment. The Hydrogel@ZIF-8 composite achieved a maximum MNZ adsorption capacity of 132.45 mg·g⁻¹, with a spontaneous and exothermic uptake process best described by a pseudo-second-order kinetic model, suggesting chemisorption as the dominant mechanism. The nanoplatform exhibited strong pH-responsive behavior, with enhanced drug release under acidic conditions and potent dose-dependent bactericidal activity against Fusobacterium nucleatum (Fn). At the highest tested concentration, bacterial survival was reduced to approximately 30%, with extensive membrane disruption observed through live/dead fluorescence microscopy. In summary, the stimuli-responsive Hydrogel@ZIF-8@MNZ nanocomposite offers an intelligent and effective therapeutic strategy for periodontitis. By tailoring its action to the disease microenvironment, this platform enables sustained and localized antibacterial therapy, addressing major challenges in the treatment of chronic oral infections. Full article

Chitosan-based hydrogels are used in the adsorption of pharmaceutical compounds from water. The adsorption process of diclofenac and naproxen on chitosan hydrogels cross-linked with glutaraldehyde has been studied theoretically and experimentally. According to the thermodynamic properties, the adsorption processes were spontaneous and endothermic, due to the negative values of Gibbs free energy, and the enthalpies of formation were positive. Furthermore, the different systems were studied by electrostatic potential maps, where the functional groups (amino and hydroxyl) represented the active sites of the hydrogel. The maximum adsorption capacity obtained for diclofenac and naproxen was 108.85 and 97.22 mg/g, respectively, at a temperature of 308.15 K. On the other hand, the adsorbent was characterized by FTIR (Fourier Transform Infrared Spectroscopy) and XRD (X-ray Diffraction) before and after the adsorption of the drugs to confirm the binding of the adsorbates on the surface of the material. Full article

Recently, researchers have been focused on the recycling as well as transforming of bio-waste streams into a valuable resource. Banana peels are promising for such application, due to their wide availability. In this context, the integration of banana peel-derived biochar with environmentally benign magnetite has significantly broadened its potential applications as a solar photocatalyst compared to the conventional photocatalysts. The materials are mixed in varied proportions of Ban-Char500-Mag@-(0:1), Ban-Char500@Mag-(1:1) and Ban-Char500@Mag-(2:1) and characterized using X-ray diffraction (XRD) and scanning electron microscopy (SEM) augmented with dispersive X-ray spectroscopy (EDX). Such modification is leading to an improvement in its application as a solar photocatalyst using the photochemical solar collector facility. The study discusses the factors controlling acetaminophen removal from aqueous effluent within 30 min of solar illumination time. Furthermore, the highlighted optimum parameters are pH 3.0, using 10 mg/L of the Ban-Char500@Mag-(1:1) catalyst and 100 mg/L of the hydrogen peroxide as a Fenton combination system for removing a complete acetaminophen from wastewater (100% oxidation). Also, the temperature influence in the oxidation system is studied and the high temperature is unfavorable, which verifies that the reaction is exothermic in nature. The catalyst is signified as a sustainable (recoverable, recyclable and reusable) substance, and showed a 72% removal even though it was in the six cyclic uses. Further, the kinetic study is assessed, and the experimental results revealed the oxidation process is following the first-order kinetic reaction. Also, the kinetic–thermodynamic parameters of activation are investigated and it is confirmed that the oxidation is exothermic and non-spontaneous in nature. Full article

This study explores the use of lignocellulosic Tectona grandis seeds (TGs), hydrochar (HC-230-4), and activated carbon (AC-850-5) produced via hydrothermal carbonization and followed by CO₂ activation for removing acridine yellow G (AYG) and acridine orange 14 (ABO) from water. HC-230-4 showed a rich presence of surface functional groups and irregular morphology with some sphere-like structures. In contrast, AC-850-5 exhibited a much higher surface area (729.7 m²/g), though with fewer surface functional groups than HC-230-4. The batch method was used to study the effects of contact time, pH, dye concentration, and temperature. Among the materials, AC-850-5 showed the highest adsorption capacity of 198 mg/g for AYG and 171 mg/g for ABO at 25 °C, around 12% higher than commercial activated carbon. The adsorption process was spontaneous and endothermic, fitting well to the Langmuir isotherm model, suggesting monolayer coverage. The adsorption kinetics followed the pseudo-second-order model, indicating that the rate depends on the surface site availability. Intraparticle diffusion analysis further confirmed a multi-step adsorption process. These findings show the strong potential of TG-derived activated carbon as an effective and sustainable material for removing acridine dyes from polluted water. Full article