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20 pages, 3410 KB  
Article
Impact of Polar Ice Layers on the Corrosion-Related Static Electric Field of a Submerged Underwater Vehicle
by Mingjie Qiu, Mingyong Hu, Yuhong Li, Dingfeng Yu and Cong Chen
Mathematics 2025, 13(17), 2882; https://doi.org/10.3390/math13172882 (registering DOI) - 6 Sep 2025
Abstract
The influence of polar ice-covered environments on the corrosion-related static electric field (CRSE) of underwater vehicles is critical for understanding and applying the characteristics of underwater electric fields in polar regions. This study utilizes a combined methodology involving COMSOL Multiphysics 6.1 simulations and [...] Read more.
The influence of polar ice-covered environments on the corrosion-related static electric field (CRSE) of underwater vehicles is critical for understanding and applying the characteristics of underwater electric fields in polar regions. This study utilizes a combined methodology involving COMSOL Multiphysics 6.1 simulations and laboratory-simulated experiments to systematically investigate the distribution characteristics of underwater vehicle electric fields under ice-covered conditions. By comparing the electric field distributions in scenarios with and without ice coverage, this study clarifies the effect of ice presence on the behavior of underwater electric fields. The simulation results demonstrate that the existence of ice layers enhances both the electric potential and field strength, with the degree of influence depending on the ice layer conductivity, thickness, and proximity of the measurement points to the ice layer. The accumulation of error analysis and laboratory experiments corroborates the reliability of the simulation results, demonstrating that ice layers enhance electric field signals by modifying the conductive properties of the surrounding medium, whereas the overall spatial distribution characteristics remain largely consistent. These findings offer a theoretical and technical basis for the optimization of stealth strategies in polar underwater vehicles and contribute to the advancement of electric field detection technologies. Full article
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22 pages, 1802 KB  
Article
Proteolytic Bacillus sp. Isolation and Identification from Tannery Alkaline Baths
by Manuela Lageiro, Fernanda Simões, Nuno Alvarenga and Alberto Reis
Molecules 2025, 30(17), 3632; https://doi.org/10.3390/molecules30173632 - 5 Sep 2025
Abstract
The application of microbial alkaline proteases holds significant potential for eco-sustainable industrial processes by reducing chemical usage and lowering the costs of effluent treatment. In the search for novel proteases with industrial relevance, several microbial strains were isolated from alkaline baths of the [...] Read more.
The application of microbial alkaline proteases holds significant potential for eco-sustainable industrial processes by reducing chemical usage and lowering the costs of effluent treatment. In the search for novel proteases with industrial relevance, several microbial strains were isolated from alkaline baths of the Portuguese tannery agroindustry. The most promising protease-producing strains were selected for identification and further study. Two isolates demonstrated the highest proteolytic activity, reaching 0.51 ± 0.01 U mL−1 and 0.70 ± 0.01 U mL−1 after 7.5 h of submerged cultivation in nutrient broth. Based on API biochemical tests, molecular biology techniques, and GC-FAME analysis of membrane lipids, the isolates were identified as Bacillus subtilis and incorporated into INIAV’s collection of industrial microbial cultures as B. subtilis CCMI 1253 (BMR2) and B. subtilis CCMI 1254 (BMR1). The most promising protease producer, B. subtilis CCMI 1253 (BMR2), exhibited a maximum specific growth rate of 0.88 ± 0.10 h−1. The proteases produced exhibited good extracellular proteolytic activity, with adaptability to industrial conditions, indicating their suitability for agroindustry applications such as leather making, detergent formulations and the treatment of effluents and protein residues. The results support the potential of microbial proteases as valuable tools in the bioeconomy and green chemistry. Full article
(This article belongs to the Special Issue Bioactive Molecules from Natural Sources and Their Functions)
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14 pages, 1498 KB  
Article
Backtracking Search Algorithm-Based Lemurs Optimizer for Coupled Structural Systems
by Khadijetou Maaloum Din, Rabii El Maani, Ahmed Tchvagha Zeine and Rachid Ellaia
Appl. Sci. 2025, 15(17), 9751; https://doi.org/10.3390/app15179751 - 5 Sep 2025
Viewed by 209
Abstract
The Backtracking Search Algorithm (BSA) has emerged as a promising stochastic optimization method. This paper introduces a novel hybrid evolutionary algorithm, termed LOBSA, integrating the strengths of BSA and Lemurs Optimizer (LO). The hybrid approach significantly improves global exploration and convergence speed, validated [...] Read more.
The Backtracking Search Algorithm (BSA) has emerged as a promising stochastic optimization method. This paper introduces a novel hybrid evolutionary algorithm, termed LOBSA, integrating the strengths of BSA and Lemurs Optimizer (LO). The hybrid approach significantly improves global exploration and convergence speed, validated through rigorous tests on 23 benchmark functions from the CEC 2013 suite, encompassing unimodal, multimodal, and fixed dimension multimodal functions. Compared with state-of-the-art algorithms, LOBSA presents a relative improvement, achieving superior results and outperforming traditional BSA by up to 35% of global performance gain in terms of solution accuracy. Moreover, the applicability and robustness of LOBSA were demonstrated in practical constrained optimization and a fluid–structure interaction problem involving the dynamic analysis and optimization of a submerged boat propeller, demonstrating both computational efficiency and real-world applicability. Full article
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19 pages, 694 KB  
Article
Mood States, Injury Status, and Countermovement Jump Performance in Brazilian High-Level Sports
by Izabel Cristina Provenza de Miranda Rohlfs, Franco Noce, Carolina F. Wilke, Tim J. Gabbett, Victoria R. Terry, Alexandre Montenegro, Carlos Alexandre Assis, Paula Moreira Magalhães, Pasteur O. de Miranda and Peter C. Terry
Sports 2025, 13(9), 303; https://doi.org/10.3390/sports13090303 - 3 Sep 2025
Viewed by 232
Abstract
This study investigated relationships between mood profiles, sports injuries, and countermovement jump with arm swing (CMJ) performance in a cohort study of 417 Brazilian athletes using a multi-methods approach during the period from January to November 2023. Six distinct mood profiles were identified, [...] Read more.
This study investigated relationships between mood profiles, sports injuries, and countermovement jump with arm swing (CMJ) performance in a cohort study of 417 Brazilian athletes using a multi-methods approach during the period from January to November 2023. Six distinct mood profiles were identified, termed the shark fin (28.3%), iceberg (20.4%), submerged (18.7%), inverse iceberg (18.0%), surface (9.8%), and inverse Everest (4.8%). Athletes with the inverse Everest profile had a significantly higher risk of injury (OR = 2.90; 90% CI [1.09–7.55]) compared to those with the iceberg profile. Random forest models showed moderate predictive capability (AUC = 0.651), with vigour (12.7%) and anger (11.5%) as primary predictors. Bayesian analysis confirmed a higher injury probability in athletes with the inverse Everest profile (31.8%). Despite statistical power limitations, the results indicate that negative mood profiles, particularly those with elevated anger and fatigue, are associated with increased injury risk. Mood scores were not associated with CMJ performance variation. These findings highlight the importance of considering mood profiles in athlete monitoring systems, acknowledging the complex interplay between psychological and physical factors in injury risk. Full article
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24 pages, 19145 KB  
Article
Marine Hydraulic Process Modelling Using SMC-Brasil on the Semi-Arid Brazilian Coast
by Thiago Cavalcante Lins Silva, Marco Túlio Mendonça Diniz, Paulo Victor do Nascimento Araújo and Bruno Ferreira
Geosciences 2025, 15(9), 344; https://doi.org/10.3390/geosciences15090344 - 3 Sep 2025
Viewed by 308
Abstract
Understanding coastal hydraulic processes is essential for sustainable coastal planning and management, especially in semi-arid regions where data scarcity represents a significant challenge. This study sought to apply the Brazilian Coastal Modelling System (SMC-Brasil) to analyse the coastal hydraulic processes present on the [...] Read more.
Understanding coastal hydraulic processes is essential for sustainable coastal planning and management, especially in semi-arid regions where data scarcity represents a significant challenge. This study sought to apply the Brazilian Coastal Modelling System (SMC-Brasil) to analyse the coastal hydraulic processes present on the Brazilian semi-arid coast in Rio Grande do Norte, seeking to understand its boundary conditions given the scarcity of data and limited monitoring network. The methodological procedures followed five main stages: data collection and processing, running the models, statistical analysis, and interpretation of the results. The simulations identified wave propagation and dissipation patterns influenced by local bathymetric features such as sandy banks and submarine canyons. The modelling indicated waves with an average Hs50% of 1.14 m, with dominant directions from ENE to ESE. Longitudinal flows ranged from 1 to 8 m3/h, with a predominance of east to west at medium and high tides. The modelling indicated spatial gradients of energy and sediment transport compatible with historical records and field observations. The results show that submerged relief irregularities play a central role in regional coastal dynamics, conditioning current flows and deposition. The application of SMC-Brasil has shown potential to fill monitoring gaps in regions with low infrastructure, offering affordable and effective technical support for adaptive coastal planning in the face of climate change impacts. Full article
(This article belongs to the Section Hydrogeology)
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53 pages, 8754 KB  
Review
Multi-Branch Towed Array System: Systematic Analysis of Modeling Methods, Environmental Responses and Mechanical Properties in Fracture Analysis
by Jin Yan, Kefan Yang, Shengqing Zeng, Keqi Yang, Dapeng Zhang and Keqiang Zhu
J. Mar. Sci. Eng. 2025, 13(9), 1697; https://doi.org/10.3390/jmse13091697 - 2 Sep 2025
Viewed by 264
Abstract
Multi-branch towed array systems are an important component of subsea information collection, which is increasingly required for subsea pipeline laying and offshore platform construction as ocean energy is exploited. However, the complexity of underwater conditions poses challenges for marine towing systems when collecting [...] Read more.
Multi-branch towed array systems are an important component of subsea information collection, which is increasingly required for subsea pipeline laying and offshore platform construction as ocean energy is exploited. However, the complexity of underwater conditions poses challenges for marine towing systems when collecting information, including the possibility of towing cable collisions with protruding seabed or submerged organisms during towing system travel, or towing cable interactions during torsion. These collisions can affect and interfere with the collection of information by the towing system, and can cause damage to the towing system or even cause the towing cable to break. After the failure and detachment of the outboard guide cable of a multi-branch towing cable array, the formation of the towing system changes, and these changes are complex and related to the prevailing sea state. To study the important condition of the damaged towing system, this paper draws an analogy between the towing system and the trawl net, and speculates the formation change and mechanical response of the multi-branch towed array system after damage by combining the influencing factors of the deployment of the towing system. Full article
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21 pages, 7413 KB  
Article
PA-MSFormer: A Phase-Aware Multi-Scale Transformer Network for ISAR Image Enhancement
by Jiale Huang, Xiaoyong Li, Lei Liu, Xiaoran Shi and Feng Zhou
Remote Sens. 2025, 17(17), 3047; https://doi.org/10.3390/rs17173047 - 2 Sep 2025
Viewed by 249
Abstract
Inverse Synthetic Aperture Radar (ISAR) imaging plays a crucial role in reconnaissance and target monitoring. However, the presence of uncertain factors often leads to indistinct component visualization and significant noise contamination in imaging results, where weak scattering components are frequently submerged by noise. [...] Read more.
Inverse Synthetic Aperture Radar (ISAR) imaging plays a crucial role in reconnaissance and target monitoring. However, the presence of uncertain factors often leads to indistinct component visualization and significant noise contamination in imaging results, where weak scattering components are frequently submerged by noise. To address these challenges, this paper proposes a Phase-Aware Multi-Scale Transformer network (PA-MSFormer) that simultaneously enhances weak component regions and suppresses noise. Unlike existing methods that struggled with this fundamental trade-off, our approach achieved 70.93 dB PSNR on electromagnetic simulation data, surpassing the previous best method by 0.6 dB, while maintaining only 1.59 million parameters. Specifically, we introduce a phase-aware attention mechanism that separates noise from weak scattering features through complex-domain modulation, a dual-branch fusion network that establishes frequency-domain separability criteria, and a progressive gate fuser that achieves pixel-level alignment between high- and low-frequency features. Extensive experiments on electromagnetic simulation and real-measured datasets demonstrate that PA-MSFormer effectively suppresses noise while significantly enhancing target visualization, establishing a solid foundation for subsequent interpretation tasks. Full article
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21 pages, 5144 KB  
Article
A Submerged Building Strategy for Low-Carbon Data Centers in Coal Mining Subsidence Areas: System Design and Energy–Carbon Performance Assessment
by Yixiao Hu, Yuben Tang, Xiang Ji and Yidong Chen
Buildings 2025, 15(17), 3148; https://doi.org/10.3390/buildings15173148 - 2 Sep 2025
Viewed by 297
Abstract
This study explores a submerged architectural strategy for data center deployment in coal mining subsidence water bodies, aiming to simultaneously address the underutilization of post-mining landscapes, the high-carbon operation of data centers, and the implementation challenges of China’s dual carbon goals. The proposed [...] Read more.
This study explores a submerged architectural strategy for data center deployment in coal mining subsidence water bodies, aiming to simultaneously address the underutilization of post-mining landscapes, the high-carbon operation of data centers, and the implementation challenges of China’s dual carbon goals. The proposed structure integrates wall-mounted plate heat exchangers into the façades of underwater data halls, using the natural convection of surrounding water as a low-grade heat sink to replace conventional cooling towers and achieve passive, low-carbon cooling. A thermal exchange model was developed based on heat transfer principles and validated by comparing outputs from TRNSYS simulations and MATLAB-based parameterized calculations, showing a deviation of less than 3% under all test conditions. The model was then used to estimate energy consumption, PUE, and carbon emissions under typical IT load scenarios. Results indicate a 42.5–64.3% reduction in cooling energy use and a 37.7–75.1% reduction in carbon emissions compared to conventional solutions, while a PUE range of 1.06–1.15 is maintained. The system also offers strong spatial adaptability and scalability, presenting a sustainable solution for redeveloping subsidence zones that supports ecological restoration and digital transformation in resource-depleted urban regions. Full article
(This article belongs to the Section Building Energy, Physics, Environment, and Systems)
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20 pages, 6348 KB  
Article
Biodiversity Assessment of the Ancient Submerged Port of Egnazia (Southern Adriatic Sea, Mediterranean Sea): New Evidence for Conservation
by Valentina Basile, Marcello Mezzasalma, Federica Talarico, Mauro Francesco La Russa and Elvira Brunelli
Fishes 2025, 10(9), 431; https://doi.org/10.3390/fishes10090431 - 2 Sep 2025
Viewed by 209
Abstract
In addition to their historical relevance, underwater sites of cultural heritage (e.g., shipwrecks, archeological sites) represent secondary habitats for marine life. However, despite a growing interest in their ecological role, studies focusing on these artificial environments remain limited. In this study, we performed [...] Read more.
In addition to their historical relevance, underwater sites of cultural heritage (e.g., shipwrecks, archeological sites) represent secondary habitats for marine life. However, despite a growing interest in their ecological role, studies focusing on these artificial environments remain limited. In this study, we performed the first systematic assessment of the marine biodiversity associated with the submerged structures of the ancient roman port of Egnazia. In particular, we conducted a seasonal (summer 2022 and winter 2023) comparative analysis of the qualitative and quantitative variations in the observed nektonic and benthic taxa between the submerged piers and two surrounding control areas. For benthopelagic and vagile benthic taxa, two seasonal samplings with four transects (of 10 m × 4 m) and three replicates per transect were conducted to analyze taxon diversity, abundance, and variability of individual size. The photo-quadrat method was employed to characterize sessile benthic taxa, using PVC frames measuring 40 cm × 40 cm, randomly placed on the study substrates with 20 replicates for each pier and the two controls. Our results highlight the occurrence of 75 taxa (67 associated with the submerged piers and 63 with the surrounding control areas), including 17 benthopelagic species, 26 vagile benthic and 32 sessile benthic taxa. Overall, our findings highlight that the submerged ancient structures of Egnazia represent a stable and functional marine habitat, promoting an increase in the local biodiversity and abundance of individuals of different species. Full article
(This article belongs to the Section Biology and Ecology)
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26 pages, 3482 KB  
Systematic Review
Constructal Design Method Applied to Wave Energy Converters: A Systematic Literature Review
by Maria Eduarda F. Capponero, Giovani D. Telli, Elizaldo D. dos Santos, Liércio A. Isoldi, Mateus das Neves Gomes, Cesare Biserni and Luiz Alberto O. Rocha
Dynamics 2025, 5(3), 36; https://doi.org/10.3390/dynamics5030036 - 1 Sep 2025
Viewed by 173
Abstract
The energy potential of sea waves has gained relevance, leading to extensive research on converters. The present work analyzes the contribution of Constructal Design to the development of wave energy converters. Constructal Design utilizes performance indicators to enhance system efficiency by varying the [...] Read more.
The energy potential of sea waves has gained relevance, leading to extensive research on converters. The present work analyzes the contribution of Constructal Design to the development of wave energy converters. Constructal Design utilizes performance indicators to enhance system efficiency by varying the degrees of freedom where flow occurs. Thus, the systematic literature review methodology was applied to gather a collection of documents focused on the research topic. This study identified articles published between 2014 and 2024 by 40 authors affiliated with institutions in Brazil, Italy, and Portugal. The oscillating water column (OWC) converter received the most research attention, followed by the overtopping converter. Analyzing the documents collected for this study, the performance indicators revealed improvements ranging from 1.19 to 839 times, indicating the lowest and highest enhancements observed, respectively. The Constructal Design method has proven highly effective in identifying specific architectures or geometric arrangements that enhance flow configuration and improve the performance of wave energy converters. However, relatively few studies have applied the Constructal Design method to wave energy converters in comparison to other methodologies, presenting a significant opportunity for future research. Full article
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18 pages, 3056 KB  
Article
A Practical 1D Approach for Real-Time Prediction of Argon Flow and Pressure in Continuous Casting of Steel
by Hyunjin Yang, Bong-Min Jin, Hyeonjin Kim, Seungwon Seo and Seunghyun Sim
Metals 2025, 15(9), 978; https://doi.org/10.3390/met15090978 - 1 Sep 2025
Viewed by 254
Abstract
The pressure and flow rate of an argon line embedded within a stopper rod serve as useful industrial indicators and control factors for mitigating air aspiration into the Submerged Entry Nozzle (SEN) during the continuous casting of steel. This manuscript investigates several challenges [...] Read more.
The pressure and flow rate of an argon line embedded within a stopper rod serve as useful industrial indicators and control factors for mitigating air aspiration into the Submerged Entry Nozzle (SEN) during the continuous casting of steel. This manuscript investigates several challenges associated with interpreting monitored argon line pressures and gas flow rates, including variations in gas pressure during delivery, actual volumes of gas entering the nozzle, argon leakage, and air aspiration. To address these issues, a new one-dimensional (1D) analytical model of compressible argon flow in the stopper rod was developed, incorporating gas dynamics and heat transfer. This concise 1D model was validated using data from a continuous casting simulator (CCS) employing a low-melting-point Bi-Sn alloy (melting point 137 °C). Pilot trials were conducted to replicate various industrial casting scenarios, generating datasets for model validation and demonstration of real-time operation. The 1D model predictions were compared with those from a CFD-based compressible flow model under CCS operating conditions. Following validation, parametric studies were conducted to explore realistic industrial scenarios (e.g., gas flow rate < 5 SLPM, nozzle diameter < 5 mm), including extreme conditions such as air aspiration and choking: a critical nozzle diameter (1.223 mm) corresponds to choked flow, limiting the maximum achievable gas flow rate to 5 SLPM. Additionally, the real-time prediction capabilities of the model were demonstrated using measured argon line pressures and flow rates from CCS trials. The proposed 1D model thus provides a practical tool for accurately interpreting SEN flow conditions from monitored argon pressures and effectively estimating argon bubble injection by clarifying actual gas pressures and flow rates at the stopper injection point. Full article
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25 pages, 4378 KB  
Article
Mechanical Properties and Microstructure of Decellularized Brown Seaweed Scaffold for Tissue Engineering
by Svava Kristinsdottir, Ottar Rolfsson, Olafur Eysteinn Sigurjonsson, Sigurður Brynjolfsson and Sigrun Nanna Karlsdottir
Bioengineering 2025, 12(9), 943; https://doi.org/10.3390/bioengineering12090943 - 31 Aug 2025
Viewed by 487
Abstract
In response to the growing demand for sustainable biomaterials in tissue engineering, we investigated the potential of structurally intact brown seaweed scaffolds derived from Laminaria digitata (L.D.) and Laminaria saccharina (L.S.), produced by a detergent-free, visible-light decellularization process aimed [...] Read more.
In response to the growing demand for sustainable biomaterials in tissue engineering, we investigated the potential of structurally intact brown seaweed scaffolds derived from Laminaria digitata (L.D.) and Laminaria saccharina (L.S.), produced by a detergent-free, visible-light decellularization process aimed at preserving structural integrity. Blades were submerged in cold flow-through and aerated water with red (620 nm) and blue (470 nm) light exposure for 4 weeks. Histology, scanning electron microscopy (SEM), and micro-computed tomography (micro-CT) analyses demonstrated that the light decellularization process removed cells/debris, maintained essential structural features, and significantly increased scaffold porosity. Mechanical property analysis through tensile testing revealed a substantial increase in tensile strength post decellularization, with L.D. scaffolds increasing from 3.4 MPa to 8.7 MPa and L.S. scaffolds from 2.1 MPa to 6.6 MPa. Chemical analysis indicated notable alterations in polysaccharide and protein composition following decellularization. Additionally, scaffolds retained high swelling and fluid absorption capacities, critical for biomedical uses. These findings underscore that the decellularized L.D. and L.S. scaffolds preserved structural integrity and exhibited enhanced mechanical properties, interconnected porous structures, and significant liquid retention capabilities, establishing them as promising biomaterial candidates for soft-tissue reinforcement, wound care, and broader applications in regenerative medicine. Full article
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17 pages, 4386 KB  
Article
A Numerical Analysis of the Fluid Flow in a Slab Mold Considering a SEN with Real Clogging and with Symmetrical Reductions
by Ariana López, Enif Gutiérrez, Saul Garcia-Hernandez, Rodolfo Morales-Dávila and Jose de Jesus Barreto
Crystals 2025, 15(9), 777; https://doi.org/10.3390/cryst15090777 - 30 Aug 2025
Viewed by 276
Abstract
Nozzle blockage has been a critical issue for productivity and product quality since the introduction of continuous casting. Despite numerous studies on the subject, the problem persists, affecting steel production. This detrimental phenomenon causes changes in the internal nozzle geometry and severe wall [...] Read more.
Nozzle blockage has been a critical issue for productivity and product quality since the introduction of continuous casting. Despite numerous studies on the subject, the problem persists, affecting steel production. This detrimental phenomenon causes changes in the internal nozzle geometry and severe wall irregularities that are neither symmetrical nor uniform. A common approach to studying the complex internal shape of clogged nozzles is considering nozzles with symmetrical transversal area reductions. Therefore, this study aims to quantitatively evaluate the effects of using realistic submerged entry nozzle (SEN) clogging geometries on the fluid dynamic behavior of molten steel inside the SEN and the mold and is compared to simplified symmetric reductions. A three-dimensional mathematical simulation based on the Navier–Stokes equations, the standard kε turbulence model, and the Volume of Fluid (VOF) method was used. The main findings indicate that symmetric reductions can only provide a qualitative prediction of the results, such as increased velocity and asymmetries at the meniscus bath level, but with errors that can reach up to 25%. Symmetric reductions fail to accurately capture the fluid dynamics inside the nozzle and the mold and should therefore be used with caution in studies that require precise flow characterization near the nozzle walls. Full article
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19 pages, 3751 KB  
Article
Application of Bovine Nasal Epithelial Cells as an In Vitro Model for Studying Viral Infection in the Upper Respiratory Tract
by Malte Pitters, Henrik Fritsch, Ang Su, Klaus Jung and Paul Becher
Viruses 2025, 17(9), 1188; https://doi.org/10.3390/v17091188 - 29 Aug 2025
Viewed by 304
Abstract
Bovine respiratory disease complex (BRDC) is a multifactorial and globally prevalent condition involving a combination of viral and bacterial pathogens, as well as environmental stressors. Viral agents often initiate infections in the upper respiratory tract (URT), predisposing animals to secondary bacterial infections and [...] Read more.
Bovine respiratory disease complex (BRDC) is a multifactorial and globally prevalent condition involving a combination of viral and bacterial pathogens, as well as environmental stressors. Viral agents often initiate infections in the upper respiratory tract (URT), predisposing animals to secondary bacterial infections and severe clinical manifestations. Among the key viral contributors to BRDC are bovine viral diarrhea virus (BVDV) and bovine herpesvirus 1 (BHV-1). In this study, submerged liquid cultures of undifferentiated bovine nasal epithelial cells (BNECs) were employed to investigate mono- and co-infections with BVDV and BHV-1. Epithelial barrier integrity was assessed to evaluate the cytopathic effects of BHV-1, while viral replication and release were quantified. Both viruses demonstrated polarized release, and BHV-1 infection exhibited a pronounced cytopathic effect. Notably, a preceding BVDV infection did not alter the progression or outcome of BHV-1 infection in this in vitro model. These findings suggest that primary BNEC cultures represent a valuable and physiologically relevant tool for studying viral dynamics and interactions associated with BRDC. Full article
(This article belongs to the Special Issue Bovine Viral Diarrhea Viruses and Other Pestiviruses)
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34 pages, 6768 KB  
Article
Functionalized Micellar Membranes from Medicinal Mushrooms as Promising Self-Growing Bioscaffolds
by Nika Kučuk, Mateja Primožič, Željko Knez and Maja Leitgeb
Polymers 2025, 17(17), 2334; https://doi.org/10.3390/polym17172334 - 28 Aug 2025
Viewed by 373
Abstract
Micellar or mycelial membranes from medicinal mushrooms are self-growing fibrous polymeric biocomposites that are biocompatible, biodegradable, cost-effective, and environmentally friendly. In this study, the cultivation process for the medicinal mushrooms Ganoderma lucidum and Pleurotus ostreatus has been optimized via submerged cultivation to maximize [...] Read more.
Micellar or mycelial membranes from medicinal mushrooms are self-growing fibrous polymeric biocomposites that are biocompatible, biodegradable, cost-effective, and environmentally friendly. In this study, the cultivation process for the medicinal mushrooms Ganoderma lucidum and Pleurotus ostreatus has been optimized via submerged cultivation to maximize growth and promote the formation of micellar membranes with high water-absorption capacity. Optimal growth conditions were achieved at an alkaline pH in a medium containing malt extract for G. lucidum, while for P. ostreatus, these were in a glucose-enriched medium. The hydrophilic underside of the micellar membranes led to a high-water uptake capacity. These membranes exhibited a broad spectrum of functional groups, thermal stability with decomposition temperatures above 260 °C, and a fibrous and porous structure. The micellar membranes from both mushrooms were additionally functionalized with mango peel extract (MPE), resulting in a uniform and gradual release profile, which is an important novelty. They also showed successful antimicrobial activity against Escherichia coli and Staphylococcus aureus growth. MPE-functionalized micellar membranes are, therefore, innovative biocomposites suitable for various biomedical applications. As they mimic the extracellular matrix of the skin, they are a promising material for tissue engineering, wound healing, and advanced skin materials applications. Full article
(This article belongs to the Section Biobased and Biodegradable Polymers)
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