Search Results (195)

A new negative-strand RNA virus was identified in grapevines from a 38-year-old ‘Chardonnay’ block in Idaho through high-throughput sequencing (HTS) of total RNA. This virus was tentatively named grapevine-associated cogu-like Idaho virus (GaCLIdV). GaCLIdV has three negative-sense, single-stranded RNA genome segments of ca. 7 kb, 1.9 kb, and 1.3 kb, encoding L protein (RNA-dependent RNA polymerase, RdRP), a movement protein (MP), and a nucleocapsid protein (NC), respectively, identified based on pair-wise comparisons with other cogu- and cogu-like viruses. In phylogenetic analysis based on the RdRP, GaCLIdV grouped within the family Phenuiviridae and was placed in a lineage of plant-infecting phenuiviruses as a sister clade of the genus Laulavirus, clustering most closely with switchgrass phenui-like virus 1 (SgPLV-1) and more distantly related to grapevine-associated cogu-like viruses from the Laulavirus and Coguvirus clades. Both GaCLIdV and SgPhLV-1 are proposed to form a new genus, Switvirus, within the family Phenuiviridae. The presence of GaCLIdV in the original ‘Chardonnay’ samples was confirmed by RT-PCR amplification and Sanger sequencing. This new virus was found in five wine grape cultivars and in six vineyards sampled in Idaho and in Oregon during the 2020–2024 seasons. GaCLIdV may have contributed to the decline observed in the old ‘Chardonnay’ block, although the role of the virus in symptom development awaits further investigation. Full article

Global warming is accelerating the poleward and upward shifts in climatically suitable ranges of species. Panicum virgatum (switchgrass) is recognized for its dual value in China’s dual-carbon strategy: mitigating food–energy land competition and restoring marginal ecosystems. However, the accuracy of habitat projections is constrained by three limitations: reliance on North American provenance data, uncalibrated model parameters, and insufficient scenario coverage. To address these, 48 switchgrass occurrence records and 22 climatic–topographic variables were integrated. The MaxEnt model was optimized with ENMeval (RM = 4.0, FC = LQH) and coupled with three SSP scenarios (SSP1-2.6, SSP3-7.0, SSP5-8.5) to quantify habitat area changes and centroid shifts across China. The key findings were as follows: (1) The mean temperature of the coldest quarter (Bio11) and elevation were identified as the key limiting factors for the suitable distribution of switchgrass, with their corresponding optimal thresholds determined as −8.79 to 8.11 °C and 0 to 2893 m, respectively. (2) The current suitable habitat covers 583.58 × 10⁴ km², concentrated in the North China Plain. (3) Under SSP5-8.5, the high-suitability habitat is projected to reach 229.44 × 10⁴ km² by the 2090s, with the centroid migrating 305 km northwestward to the Inner Mongolia–Jilin belt. This study highlights the climate–topography coupling that drives northward migration and proposes cold-tolerant cultivar development, priority zoning of marginal lands, and ecological corridor establishment to inform climate-smart biomass energy planning in China. Full article

The pursuit of sustainable alternatives to portland cement has become a global imperative within the construction sector, driven by the need to reduce carbon dioxide emissions and energy consumption. Among the promising alternatives, agricultural ashes have garnered attention for their potential as alternative supplementary cementitious materials (ASCMs), owing to their inherent pozzolanic properties when appropriately processed. However, the availability and utilization of these ashes have predominantly been concentrated in tropical and subtropical regions, where such biomass is more abundant. This review offers a comprehensive bibliometric analysis to identify and assess agricultural ashes (specifically switchgrass, barley, sunflower, and oat husks) that are cultivated in temperate and cold climates and exhibit potential for SCM application. The analysis aims to bridge the knowledge gap by systematically mapping the existing research landscape and highlighting underexplored resources suitable for cold-region implementation. Key processing parameters, including incineration temperature, retention duration, and post-combustion grinding techniques, are critically examined for their influence on the resulting ash’s physicochemical characteristics and pozzolanic reactivity. In addition, the effect on fresh, hardened, and durability properties was evaluated. Findings reveal that several crops grown in colder regions may produce ashes rich in reactive silica, thereby qualifying them as viable ASCM candidates and bioenergy sources. Notably, the ashes derived from switchgrass, barley, oats, and sunflowers demonstrate significant reactive silica content, reinforcing their potential in sustainable construction practices. Hence, this study underscores the multifaceted benefits of contributing to the decarbonization of the cement industry and circular economy, while addressing environmental challenges associated with biomass waste disposal and uncontrolled open-air combustion. Full article

In the bioenergy industry, highway hauling cost is typically 30%, or more, of the average cost of feedstock delivered to a biorefinery. Thus, truck productivity, in terms of Mg/d/truck, is a key issue in the design of a logistics system. One possible solution to this problem that is being explored is the utilization of modular pellet depots. In such a logistics system, raw biomass (i.e., low-bulk-density product) is converted into pellets (i.e., high-bulk-density product) by several smaller-scale modular pellet depots instead of by a single larger-capacity pellet depot. A truckload of raw biomass (e.g., round bales) is 16 Mg and a load of pellets is 34 Mg. The distribution of depots across a feedstock production area can potentially have an impact on the total truck operating hours (i.e., raw biomass hauling to a depot + pellet hauling from the depot to the biorefinery) required to deliver feedstock for annual operation of a biorefinery. This study examined three different distributions of depots across five feedstock production areas. The numbers of depots were one, two, and four per production area for totals of five, ten, and twenty depots. Increasing the number of depots from five to ten reduced raw biomass hauling hours by 12%, and increasing from five to twenty reduced these hours by 30%. Total hauling hours (raw biomass + pellets) were reduced by less than 1% with an increase from five to ten and by about 11% with an increase from five to twenty. The modular pellet depot concept demonstrated potential for providing improvements to biorefinery logistics systems, but more research is needed to optimize this balance. Full article

Interest in using warm-season grasses, including switchgrass (SG) (Panicum virgatum L.), as a bioenergy crop has increased in Europe. This study evaluated the effects of harvesting regimes with two cuts per year on the productivity, chemical composition and biochemical methane potential of the SG cultivars ‘Dacotah’, ‘Foresburg’ and ‘Cave in Rock’ in environments with cool and moderate climates in Europe with minimal fertilizer application. The results of two harvest years suggest that the biomass yield, chemical composition and energy potential depend on the grass cultivars and harvesting time. Significant effects (p < 0.05) of the harvest date and cultivar were observed for most of the measured parameters for biomass and silage quality. All three SG cultivars harvested on August 8 produced the lowest (p < 0.05) volume of methane per kg of biomass (181–202 normal litres (NL) per kg⁻¹ volatile solids (VS)) compared to the biomass of the respective cultivar harvested on 14 July (287–308 NL kg⁻¹ VS) or on October 3, as regrowth after the first cut made in mid-July (274–307 NL kg⁻¹ VS). The stands of all three SG cultivars, when the first harvest was completed in mid-July, achieved a higher annual area-specific methane yield than those harvested first in August (1128–1900 Nm³ ha⁻¹ and 888–1332 Nm³ ha⁻¹, respectively). Depending on the harvest regime and cultivar, the annual gross energy presented as a lower heating value varied from 31.8 GJ ha⁻¹ to 68.0 GJ ha⁻¹. It is concluded that SG growing under the cool temperate climate of Northern Europe could be an interesting alternative crop for methane production. Our study proves that the cultivar choice also plays an important role. Full article

The increasing demand for sustainable materials in automotive applications, coupled with the critical need to address marine plastic pollution, presents an opportunity for innovative material development. This study explores composites made from recycled polyamide 6 (PA6) fishing nets reinforced with switchgrass fibers (0–30 wt%). The composite with 30 wt% switchgrass fibers increased tensile strength by 23% and Young’s modulus by 126% compared to unreinforced recycled PA6, achieving 93% of the tensile strength of commercial automotive-grade neat PA6 and surpassing another grade by 22%. However, higher fiber loading hindered processability, as evidenced by incomplete mold filling and reflected by a decrease in melt flow rate from 19.35 to 8.63 g/10 min. Thermal analysis revealed reduced crystallinity and crystallization temperatures with fiber addition, attributed to restricted polymer chain mobility. While dynamic mechanical analysis demonstrated improved stiffness below the glass transition temperature, scanning electron microscopy indicated optimal fiber-matrix adhesion at up to 20 wt% fiber loading, with aggregation at higher concentrations. These findings establish recycled fishing net-derived PA6/switchgrass fiber composites as a viable alternative to virgin materials in automotive applications, with mechanical properties comparable to commercial grades. Although the composites demonstrate enhanced mechanical strength and modulus, the significant reduction in ductility restricts their use to rigid, semi-structural components where flexibility is not critical. Future research should address processing challenges to enhance fiber dispersion and interfacial adhesion at higher loadings. Full article

Pinios river basin constitutes the most important agricultural production area in Greece but contributes to the degradation of the quality and quantity of surface water and groundwater bodies. Bioenergy crops implemented as part of the existing cropping systems could be a novel and efficient mitigation strategy against water degradation, contributing to the production of energy through renewable sources. This study uses the Soil and Water Assessment Tool (SWAT) to first develop a representative model of Pinios river basin and evaluate its current state with respect to water availability and nitrate water pollution. A low-input perennial bioenergy crop, switchgrass, is then simulated closely to the Greek conditions to investigate its potential effects on water in three implementation scenarios: the installation and growth of switchgrass in the entire irrigated cropland, exclusively in irrigated sloping (slopes > 1.5%) cropland, and exclusively in irrigated non-sloping cropland. The simulated results demonstrate that under all scenarios, the water quality improvements with respect to the nitrate loads entering surface water and groundwater bodies were significant, with their reduction being directly affected by the extent to which switchgrass replaced resource-demanding conventional crops. Specifically, the reduction in the annual nitrate loads in the surface water under these three scenarios varied from 7% to 18% at the river basin scale, while in certain cropland areas, the respective reduction even exceeded a level of 80%. The potential to improve the water status was also considerable, as the implementation of the bioenergy crop reduced the irrigation water used annually in the basin by 10% (64 Mm³) when switchgrass replaced the conventional crops only on the sloping land and by almost 30% (187 Mm³) when it replaced them throughout the irrigated land. At the same time, significant biomass production above 18 t/ha/y applied in all of the simulations. This study also highlights the contribution of the bioenergy crop to the rehabilitation of the groundwater levels across the basin, with the possibility of increasing them by >50% compared to the baseline, implying that the adoption of switchgrass could be a promising means against water scarcity. Full article

Data are needed to identify optimum response to potassium (K) and phosphorus (P) amendment and associated mycorrhizal colonization for native warm-season grasses (NWSGs; big bluestem [BB; Andropogon gerardii Vitman] and switchgrass [SG; Panicum virgatum L.]). To evaluate these responses, experiments were conducted in Knoxville and Springfield, Tennessee, from 2013 to 2019. In twice-annual harvests, we assessed BB and SG dry matter (DM) yield, crude protein (CP), total digestible nutrients (TDNs), P and K removed by grasses (removal), and soil test P and K in response to P (29 to 88 kg ha⁻¹) and K (70 to 257 kg ha⁻¹) elemental rates, and rates of root colonization by mycorrhizal fungi in response to P. Amendments had no effect (p > 0.05) on DM yield, CP, or TDN for either species. Yield, CP, and TDN fluctuated among years (p < 0.001) for both species, but no consistent temporal trends were observed. Although removal exceeded inputs at the control (no input) for P and K, and at 70 kg K ha⁻¹, there was not an associated reduction in soil test K and P values. Phosphorus rate affected (p = 0.02) total mycorrhizal colonization, with an average of 62% colonization across both species and 70% at the highest P rates. Given the lack of response for yield, CP, TDN, or associated soil nutrient test levels, NWSGs appear to offer a low-input option for forage production. Full article

Switchgrass (Panicum virgatum L.) is an important cellulosic biofuel grass native to North America. Rust, caused by Puccinia spp. is the most predominant disease of switchgrass and has the potential to impact biomass conversion. In this study, virulence patterns were determined on a set of 38 switchgrass genotypes for 14 single-spore rust isolates from 14 field samples collected in seven states. Single nucleotide polymorphism (SNP) variation was also assessed in 720 sequenced cloned amplicons representing 654 base pairs of the elongation factor 1-α gene from the field samples. Five major haplotypes were identified differing by 11 out of the 39 SNP positions identified. STRUCTURE, Principal Coordinate Analysis, and phylogenetic analyses divided the rust population into two genetic clusters. Virginia and Georgia had the highest and lowest rust genetic diversity, respectively. Only nine accessions showed a differential disease response between the 14 isolates, allowing the identification of eight races, differing by 1–3 virulence factors. Overall, the results suggested clonal reproduction of the pathogen and a North–South differentiation via local adaptation. However, similar haplotypes and races were also recovered from several states, suggesting migration events, and highlighting the need to further investigate the switchgrass rust population structure and evolution in the USA. Full article

Innovative methods for estimating commercial-scale switchgrass yields and feedstock quality are essential to optimize harvest logistics and biorefinery efficiency for sustainable aviation fuel production. This study utilized vegetation indices (VIs) derived from multispectral images to predict biomass yield and lignocellulose concentrations of advanced bioenergy-type switchgrass cultivars (“Liberty” and “Independence”) under two N rates (28 and 56 kg N ha⁻¹). Field-scale plots were arranged in a randomized complete block design (RCBD) and replicated three times at Urbana, IL. Multispectral images captured during the 2021–2023 growing seasons were used to extract VIs. The results show that linear and exponential models outperformed partial least square and random forest models, with mid-August imagery providing the best predictions for biomass, cellulose, and hemicellulose. The green normalized difference vegetation index (GNDVI) was the best univariate predictor for biomass yield (R² = 0.86), while a multivariate combination of the GNDVI and normalized difference red-edge index (NDRE) enhanced prediction accuracy (R² = 0.88). Cellulose was best predicted using the NDRE (R² = 0.53), whereas hemicellulose prediction was most effective with a multivariate model combining the GNDVI, NDRE, NDVI, and green ratio vegetation index (GRVI) (R² = 0.44). These findings demonstrate the potential of UAV-based VIs for the in-season estimation of biomass yield and cellulose concentration. Full article

With rising demand for wood products and reduced wood harvesting due to the European Green Deal, alternative lignocellulosic materials for insulation are necessary. In this work, we manufactured reference particleboard from industrial particles and fifteen different board variants from alternative lignocellulosic plants material, i.e., five types of perennial plant biomass in three substitutions: 30, 50 and 75% of their share in the board with a nominal density of 250 kg/m³. Within the analysis of manufactured boards, the mechanical, chemical and thermal properties were investigated—internal bond, formaldehyde emissions, thermal insulation, heat transfer coefficient and thermal conductivity. In the case of thermal conductivity, the most promising results from a practical point of view (W/mK < 0.07) were obtained with Sida hermaphrodita and Miscanthus, achieving the best results at 50% substitution. The lowest formaldehyde emissions were recorded for boards with Panicum virgatum and Miscanthus, highlighting their positive environmental performance. In terms of mechanical properties, the highest internal bond was noticed in particleboards with a 30% substitution of Spartina pectinata and Miscanthus. Research findings confirm the potential of perennial plants as a sustainable source of raw materials for insulation panel manufacturing. Despite needing improvements in mechanical properties, most notably internal bond strength, these plants offer an ecologically responsible solution aligned with global construction trends, thus lessening reliance on traditional wood products. Thus, long-term benefits may be realized through the strategic combination of diverse raw materials within a single particleboard. Full article

Biomass can be used for electricity generation, especially in developing countries, but also in developed ones, where the utilization of renewable energy sources is being integrated into a sustainable economy. There are considerable differences in the scale of biomass use and in the technology of its processing. One of the most important sources of biofuel is the biomass of grass. This research aimed to determine the long-term effects of organic fertilizers on cellulose, hemicellulose, and lignin content in the biomass of three grass species: giant miscanthus (Miscanthus × giganteus), prairie cordgrass (Spartina pectinata), and switchgrass (Panicum virgatum L.) in the first three years of growth. The experiment was established in four replications on microplots of 2 m² in April 2018. Before planting grass rhizomes, municipal sewage sludge (SS) and spent mushroom substrate (SMS) were introduced into the soil in various combinations. Biomass is harvested in December every year. The content of structural polysaccharides in the grass species statistically significantly varied in response to organic waste. Compared to other fertilizer combinations, SS application increased the content of cellulose in the biomass of Miscanthus giganteus (43.66% of DM) and Spartina pectinata (37.69% of DM) and hemicellulose in Spartina pectinata (27.80% of DM) and Panicum virgatum (23.64% of DM). Of the three species of grass, the chemical composition of Miscanthus giganteus cell walls was the most favorable for biofuel production, with the most cellulose and hemicellulose and the least lignin compared to other grass species. The content of lignin in the biomass of Miscanthus × giganteus and Spartina pectinata was the greatest on the plot with SMS and amounted to 7.79% of DM and 12.32% of DM, respectively. In the case of Panicum virgatum, the average content of lignin was similar across all fertilized plots, with 15.42% DM. Full article

This paper investigates bioethanol production from switchgrass, focusing on enhancement of efficiency through various pretreatment methods and comparing two bioethanol production processes: simultaneous saccharification and fermentation (SSF) and separate hydrolysis and fermentation (SHF). Physical, chemical, and biological pretreatment processes are applied to enhance the breakdown of switchgrass’s lignocellulosic structure. Effects of pretreatments, enzymatic hydrolysis, and fermentation on ethanol yield are discussed in detail. The comparative analysis reveals that SSF yields higher ethanol outputs within shorter times by integrating hydrolysis and fermentation into a single process. In contrast, SHF offers more control by separating these stages. The comparative analysis highlights that SSF achieves higher ethanol yields more efficiently, although it might restrict SHF’s operational flexibility. This study aims to provide a comprehensive overview of the current pretreatments, hydrolysis methods, and fermentation processes in bioethanol production from switchgrass, offering insights into their scalability, economic viability, and potential environmental benefits. The findings are expected to contribute to the ongoing discussions and developments in renewable bioenergy solutions, supporting advancing more sustainable and efficient bioethanol production techniques. Full article

The dwindling availability of agricultural land, caused by factors such as rapid population growth, urban expansion, and soil contamination, has significantly increased the pressure on food production. To address this challenge, cultivating non-food crops on contaminated land has emerged as a promising solution. This approach not only frees up fertile soil for food production but also mitigates human exposure to contaminants. This work aimed to examine the impact of soil contamination with Cd, Pb, Ni, and Zn on the growth, productivity, metal accumulation, and the tolerance of five lignocellulosic non-food crops: switchgrass (Panicum virgatum L.), biomass sorghum (Sorghum bicolor L. Moench), giant reed (Arundo donax L.), African fodder cane (Saccharum spontaneum L. spp. aegyptiacum Willd. Hackel), and miscanthus (Miscanthus × giganteus Greef et Deu.). A two-year pot experiment was conducted in Greece, Italy, and Portugal, following the same protocols and applying various levels of metals: Cd (0, 4, 8 mg kg⁻¹), Pb and Zn (0, 450, 900 mg kg⁻¹), and Ni (0, 110, 220 mg kg⁻¹). The experimental design was completely randomized, with three replicates for each treatment. The results showed that switchgrass and sorghum generally maintained their height and productivity under Cd and Pb stress but were adversely affected by high Zn and Ni concentrations. Giant reed and African fodder cane showed reduced height and productivity at higher Ni and Zn levels. Miscanthus exhibited resilience in height but experienced productivity reductions only at the highest Zn concentration. Heavy metal uptake varied among crops, with switchgrass and sorghum showing high Cd and Pb uptake, while giant reed accumulated the most Cd and Zn. Miscanthus had the highest Ni accumulation. The tolerance indices indicated that switchgrass and sorghum were more tolerant to Cd and Zn at lower concentrations, whereas miscanthus had lower tolerance to Cd but a higher tolerance to Zn at higher concentrations. Giant reed and African fodder cane demonstrated stable tolerance across most heavy metals. Accumulation indices highlighted the effectiveness of switchgrass and sorghum in Cd and Pb uptake, while miscanthus excelled in Ni and Zn accumulation. The cluster analysis revealed similar responses to heavy metal stress between African fodder cane and giant reed, as well as between sorghum and miscanthus, with switchgrass displaying distinct behavior. Overall, the study highlights the differential tolerance and accumulation capacities of these crops, indicating the potential for phytoremediation applications and biomass production in heavy metal-contaminated soils. Full article

Lignin is endowed with antioxidant activity due to its diverse chemical structure. It is necessary to explore the relationship between antioxidant activity and the chemical structure of the lignin to develop its high-value utilization. Herein, we employed maleic acid (MA) as a hydrotropic agent to preferably isolate the lignin from distinct herbaceous sources (wheat straw and switchgrass) under atmospheric pressure conditions. The resultant acid hydrotropic lignin (AHL) isolated from wheat straw exhibited high radical scavenging rates, up to 98% toward DPPH and 94% toward ABTS. Further investigations indicated that during the MA hydrotropic fractionation (MAHF) process, lignin was carboxylated by MA at γ-OH of the side-chain, providing additional antioxidant activity from the carboxy group. It was also found that the radical scavenging rate of AHL has a positive correlation with carboxyl, phenolic hydroxyl contents, and the S–G (syringyl–guaiacyl) ratio, which could be realized by increasing the MAHF severity. Overall, this work underlies the enhancement origin of the antioxidant property of lignin, which will facilitate its application in biological fields as an efficient, cheap, and renewable antioxidant additive. Full article