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Keywords = tapered fiber bioprobes

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12 pages, 12196 KiB  
Article
Tapered Fiber Bioprobe Based on U-Shaped Fiber Transmission for Immunoassay
by Xinghong Chen, Lei Xiao, Xuejin Li, Duo Yi, Jinghan Zhang, Hao Yuan, Zhiyao Ning, Xueming Hong and Yuzhi Chen
Biosensors 2023, 13(10), 940; https://doi.org/10.3390/bios13100940 - 20 Oct 2023
Cited by 3 | Viewed by 2329
Abstract
In this paper, a tapered fiber bioprobe based on Mach–Zehnder interference (MZI) is proposed. To retain the highly sensitive straight-tapered fiber MZI sensing structure, we designed a U-shaped transmission fiber structure for the collection of optical sensing signals to achieve a miniature-insert-probe design. [...] Read more.
In this paper, a tapered fiber bioprobe based on Mach–Zehnder interference (MZI) is proposed. To retain the highly sensitive straight-tapered fiber MZI sensing structure, we designed a U-shaped transmission fiber structure for the collection of optical sensing signals to achieve a miniature-insert-probe design. The spectrum responses from the conventional straight-tapered fiber MZI sensor and our proposed sensor were compared and analyzed, and experimental results showed that our proposed sensor not only has the same sensing capability as the straight-tapered fiber sensor, but also has the advantages of being flexible, convenient, and less liquid-consuming, which are attributed to the inserted probe design. The tapered fiber bioprobe obtained a sensitivity of 1611.27 nm/RIU in the refractive index detection range of 1.3326–1.3414. Finally, immunoassays for different concentrations of human immunoglobulin G were achieved with the tapered fiber bioprobe through surface functionalization, and the detection limit was 45 ng/mL. Our tapered fiber bioprobe has the insert-probe advantages of simpleness, convenience, and fast operation. Simultaneously, it is low-cost, highly sensitive, and has a low detection limit, which means it has potential applications in immunoassays and early medical diagnosis. Full article
(This article belongs to the Special Issue Recent Advances in Optical Fiber Biosensor)
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