Search Results (755)

Children are often underserved by adult-oriented oral medicines, leading to off-label use and dosage-form manipulation that may compromise dosing accuracy. This review summarises recent advances in paediatric orodispersible tablets (ODTs), focusing on manufacturing technologies, superdisintegrants, taste masking, and in vitro disintegration testing. Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidance and a protocol registered with the International Platform of Registered Systematic Review and Meta-analysis Protocols (registration number INPLASY2025110022), we searched PubMed, EMBASE, MEDLINE, Scopus, and Google Scholar for experimental studies on paediatric-relevant ODT formulation and evaluation. Two reviewers screened studies and extracted data on manufacturing methods, excipients, disintegration/dissolution testing, and key outcomes. Risk of bias was assessed using a six-domain framework. Overall, 65 studies met the inclusion criteria for this review. Direct compression was the dominant method, with freeze-drying, sublimation, spray-drying, nanoparticle-in-tablet systems, and semi-solid extrusion/3D printing also reported. Crospovidone, croscarmellose sodium, and sodium starch glycolate were the most common superdisintegrants, while natural and co-processed disintegrants showed promise as cost-effective alternatives. Disintegration was usually assessed using pharmacopoeial methods, with some modified set-ups to better simulate oral conditions. Paediatric ODT development is advancing rapidly. Broader translation requires harmonised disintegration testing, age-stratified acceptability reporting, and GMP-ready workflows, alongside benchmarking of superdisintegrants and attention to dose flexibility, packaging, and affordability. Full article

Bitter taste receptors (TAS2Rs) are G protein-coupled receptors best known for detecting bitter compounds in the oral cavity. However, their expression patterns and physiological roles in the liver remain largely unexplored. Here, we employed molecular and immunohistochemical approaches to demonstrate that multiple TAS2Rs are expressed in human Hep3B cells and mouse primary hepatocytes (MPHs) and co-localized with β-adrenergic receptors (βARs) at the bile canaliculi. Bioluminescence resonance energy transfer (BRET), cAMP assays, and Western blot analyses revealed that certain TAS2Rs exhibit ligand-dependent coupling preferences for the G protein subunits Gαi1, Gαi2, and Gαi3. This coupling leads to inhibition of cAMP production and a reduction in protein kinase A (PKA) substrate phosphorylation. Biochemical assays further showed that TAS2R activation significantly attenuates βAR-mediated lipolysis, as well as the production of glycerol and free fatty acid in both Hep3B cells and MPHs. These effects were partially reversed by small interfering RNA (siRNA)-mediated knockdown of TAS2Rs. Moreover, studies using a steatotic mouse model demonstrated that bitter compounds inhibit lipid droplet degradation, resulting in hepatic triacylglycerol accumulation. Collectively, these findings reveal a role for TAS2Rs in modulating hepatic lipid metabolism and highlight their potential as therapeutic targets for the prevention and treatment of liver diseases. Full article

Raisins come from dried Vitis vinifera L. grapes. They are consumed worldwide, and their shape, color, texture, and taste largely determine consumer preference and market success. Consumers often select raisins based on visual appeal—namely color—without insight into how this relates to nutritional quality. Therefore, this study evaluated raisins of different colors based on non-targeted metabolomics to reveal the nutritional differences among differently colored raisins and to measure the differences in antioxidant capacity. Compared with green raisins (‘Sultanina’), 377–381 differential metabolites were identified in other colored varieties. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that these metabolites were enriched in pathways such as ‘biosynthesis of other secondary metabolites’ and ‘amino acid metabolism’. The comparison of the antioxidant capacity of raisins of different colors shows that the darker the color of the raisins, the stronger their antioxidant capacity. Correlation analysis between total antioxidant capacity and 14 differential metabolites showed a significant positive correlation. Notably, syringetin levels in black raisins (‘Blackcurrant’ and ‘Sweet Sapphire’) were substantially higher—148.31 and 515.94 times greater, respectively—than in green raisins (‘Sultanina’). This elevated syringetin content may significantly contribute to the enhanced antioxidant capacity of black raisins. Furthermore, based on the positive ion mode, the relative contents of 24 and 12 differential metabolites were relatively high in green and red raisins, respectively. The negative ion model identified that 19 and 4 differential metabolites had relatively high contents in green and red raisins. These metabolites may be linked to the unique health benefits of red and green raisins. This study provides valuable insights for consumers selecting raisins based on health needs and for companies developing raisin-based health products. Full article

Background: Encouraging fruit and vegetable (FV) consumption early in childhood is important for long-term healthy eating. Though parents play an important role in shaping children’s FV-related taste preferences and consumption, validated instruments assessing the range of parenting practices that specifically support young children’s FV intake are scarce. Furthermore, little attention has been given to low-income families, cultural inclusivity, and FV practices across different settings. The current study sought to conduct an initial examination and explore the measurement equivalency of a new FV parenting practices questionnaire (FVPPQ) across racially/ethnically diverse groups that address these gaps. Methods: Data for this paper came from a large project focused on parents’ FV parenting practices with young children enrolled in Head Start programs in the southern part of the U.S. Inclusion criteria were (a) parent/legal guardian being eighteen or older, (b) being the primary person responsible for child feeding, and (c) the child not requiring a special diet (e.g., diabetic). Using a multi-phases project approach, we (1) developed a preliminary conceptual map of parenting practice domains by reviewing existing measures on FV parenting practices; (2) completed and content-analyzed data from 18 focus groups (n = 62) to identify and further revise the preliminary conceptual map of domains, (3) administered a questionnaire with 11 domains of FV parenting practices, and then (4) empirically explored and reduced the measure while evaluating its content, construct, and criterion validity, and cultural equivalency across Non-Hispanic White, Hispanic White, and Black parents (n = 281). Results: Findings from Phases 1 and 2 generated a 107-item questionnaire that was reduced during phase 3 through a series of principal component and confirmatory factor analyses to the final FVPPQ with 21 items in four unique domains, showing good variability and inter-item consistency reliability: (1) Availability (5 items); (2) modeling (5 items); child-focused (5 items); and pressure (6 items). Three of the four domains evidenced cultural equivalency. Conclusions: The FVPPQ with four unique subscales demonstrated good content, construct validity, and partial measurement equivalency across racially/ethnically diverse groups of parents. Further confirmatory validation is warranted in larger samples, but the FVPPQ might be a promising and easily administered measure for research and applied interventions in nutrition, health behavior, and parenting contexts. Full article

Background: The UCP1 A-3826G polymorphism, located in the gene’s regulatory region, is associated with obesity and altered fat metabolism. Because UCP1 plays a central role in thermogenesis, variation in its expression may influence metabolic efficiency and dietary fat preference. Methods: We examined associations between the A-3826G polymorphism and food preferences in healthy young Japanese adults (50 males, 48 females). Preferences for high-fat and basic-taste foods were assessed using a self-administered questionnaire, with sweet foods classified as low- or high-fat. Genotypes (AA, AG, GG) were analyzed using a two-way mixed-design ANOVA to evaluate genotype × fat level interactions. Results: Preference scores for basic tastes did not differ significantly among genotypes in either sex (except for sour taste in males). In males, no significant genotype × fat level interaction was observed, although AA carriers preferred high-fat to low-fat sweet foods (p < 0.05). In females, a significant genotype × fat level interaction was detected (p < 0.01), with AG carriers showing lower preference for high-fat sweet foods. Conclusions: These findings indicate that the UCP1 A-3826G polymorphism may modulate preference for high-fat sweet foods in a sex-dependent manner, suggesting a link between thermogenic genetic variation and dietary fat preference relevant to obesity prevention. Full article

Chinese Americans who smoke have low use of nicotine replacement therapy (NRT). This study explored perceptions of NRT and decision-making around product choice among Chinese American smokers who received NRT. From September 2023 to January 2024, we conducted in-depth phone interviews with 20 participants recruited in New York City from a WeChat-based cessation pilot trial and a community-based cessation program, both providing free nicotine patches, gum, or lozenges. Participants (aged 26–72; 85% male; 60% current smoking) included 12 consistent NRT users (≥2 weeks), four trial users (<2 weeks), and four non-users. Five participants (25%) had never heard of NRT before program enrollment, and 14 (70%) had never used it previously. Consistent users generally viewed NRT as helpful in reducing cravings. Others reported barriers, including culturally rooted skepticism toward pharmacotherapy, preference for unassisted quitting, lack of readiness to quit, prior negative experiences, and unpleasant taste or side effects. Product choice was influenced by lay knowledge, ease of integrating NRT into daily routines, perceived effectiveness, and taste and side effects. Cessation programs addressing cultural beliefs, reframing willpower, engaging individuals not ready, and providing diverse NRT options with guidance on side effect management and routine integration may increase NRT use among this population. Full article

Background/Objectives Foods for special medical purposes are an important component of nutritional management in patients at risk of malnutrition or already affected by it. The aim of the study was to evaluate the sensory properties and sensory acceptability of selected powdered foods for special medical purposes (FSMPs) from a single manufacturer with different flavour variants using quantitative descriptive analysis. Methods: The study was conducted under laboratory conditions in accordance with the PN-EN ISO 8589:2010 standard. A semi-trained panel of 49 participants took part in the sensory evaluation. Five powdered FSMP products with comparable nutritional composition and different flavour variants were analyzed. Quantitative descriptive analysis (QDA) was applied using a 10-point rating scale, along with a ranking method to assess consumer preferences. Statistical analysis was performed using Welch’s one-way analysis of variance followed by post hoc tests, with the level of significance set at p < 0.05. Results: Statistically significant differences were observed between the analyzed variants for most of the assessed sensory attributes. Preparations with white chocolate and raspberry, strawberry, and vanilla flavours showed the highest sensory acceptability, characterized by a harmonious taste, creamy texture, and low intensity of undesirable sensory attributes. The neutral variant received the lowest overall ratings. The coffee-flavoured product was distinguished by a high aroma intensity but also by a greater intensity of attributes negatively affecting sensory acceptability. Conclusions: The findings indicate that flavour plays a significant role in determining the sensory acceptability of FSMP products. Considering sensory characteristics in the development and selection of FSMP preparations may increase their consumption and enhance the effectiveness of nutritional interventions in clinical practice. Full article

Background: Childhood and adolescence represent a critical period for shaping long-term dietary habits, including whole grain consumption, which remains low despite well-documented health benefits. Objective: This cross-sectional study (November–December 2024) examined Slovenian adolescents’ attitudes toward whole-grain foods in the context of organized school meals. Methods: Participants aged 10–12 years and 14–19 years (N = 501; mean age 15.6 ± 2.6) completed an online questionnaire assessing knowledge, self-reported consumption frequency, preferences, motivational factors, and food neophobia using the translated Italian Child Food Neophobia Scale (ICFNS). Based on ICFNS scores, participants were classified as low (≤17), medium (18–24), or high (≥25) in food neophobia. Results: Older adolescents demonstrated better knowledge of whole-grain health benefits; however, greater knowledge was not associated with higher self-reported consumption. Food neophobia was strongly associated with lower consumption frequency and reduced willingness to try whole-grain foods, including whole-grain bread, oatmeal, buckwheat porridge and brown rice. Across all groups, taste was the most consistent motivator for trying whole-grain foods. Older adolescents prioritized health and appearance as key reasons for eating more whole grain foods. Conclusions: Findings suggest that improving taste, increasing exposure, and leveraging institutional settings such as schools, where availability, preparation, and social cues can be managed, may be effective in promoting whole-grain food consumption. Full article

The growing demand for clean-label bakery products requires a deeper understanding of how functional ingredients and physicochemical properties shape consumer perception. This study characterized nine commercial clean-label breads formulated with alternative flours, oilseeds, and functional ingredients by integrating instrumental analyses (color, porosity, free amino acids, total phenolic content, antioxidant activity) with consumer evaluation using hedonic testing and Check-All-That-Apply (CATA). Sixty-five consumers evaluated the breads under blind conditions. Results showed that flour type and seed inclusion significantly affected color, structure, and bioactive compound levels. Breads with higher phenolic content and antioxidant activity (GB-B, GB-C, GB-D, PB-I) exhibited more complex aroma profiles, whereas breads with higher porosity (GB-A, PB-G) were perceived as softer. Taste and texture showed the strongest correlation with overall liking (r > 0.84). CATA and penalty analysis identified soft, easy to chew, sweet, and umami as key drivers of liking, while dry, adhesive, bran odor, and bitter negatively impacted acceptance. Data revealed that consumer preference depends on the balance between structural attributes, flavor development, and nutritional composition. These findings provide actionable insights for the formulation of clean-label breads that balance health benefits and sensory acceptance. Full article

A growing body of evidence indicates that the gut microbiota has a role in the mating preference process in mammals. This likely occurs through the modulation of various mating signals induced by symbiotic bacteria, thereby leading to variations in mating behavior. Given that giant pandas are solitary wild animals that rely on chemical signals for mate selection, it is relevant to explore whether the mating behavior of giant pandas is also affected by the gut microbiota. We hypothesize that antibiotic treatment-induced residual antibiotic resistance genes in captive giant pandas may disrupt intestinal microbiota homeostasis, diminish the abundance of beneficial microorganisms involved in short-chain fatty acid synthesis, and consequently impair nervous system function via the gut–brain axis. The ensuing physiological stress is likely to suppress innate mating behavior and compromise pheromone synthesis, thereby reducing an individual’s attractiveness to potential mates. To answer this question, we utilize fecal metagenomics technology to analyze the differences in gut microbes and antibiotic resistance genes (ARGs) between captive male adult giant pandas displaying natural versus non-natural mating behavior. The research findings suggest that, when compared with captive adult male giant pandas demonstrating natural mating behavior, those with non-natural mating behavior exhibit a significantly reduction in the abundance of beneficial gut microorganisms (s_Clostridium sp. and f_Ruminococcaceae) (p < 0.05). Concurrently, there is a significantly increase in the observed resistance genes tetO and mgtA, which are mainly associated with macrolide and tetracycline resistance (p < 0.05). Furthermore, Kegg functional analysis reveals a significant up-regulation of metabolic pathways related to sensory systems, such as taste and olfactory transduction, in the intestines of captive adult male giant pandas showing natural mating behavior. These results imply that changes in the abundance of gut microbiota and ARGs are correlated with the manifestation of natural mating behavior in captive adult male giant pandas. Consequently, to improve the success rate of natural reproduction within the male giant panda populations in captive environments, it is advisable to administer antibiotics judiciously and closely monitor the composition of beneficial bacteria in their gut microbiota. The findings of this study provide novel perspectives on the mechanisms by which captive conditions affect the decline in natural mating behavior observed in adult male giant pandas. Full article

Objectives: This study investigated the impact of age on the diagnostic accuracy and patient acceptance of two chewing efficiency tests: the digital Mini Dental Assessment (MDA) using carrots and the CHEW test by Slavicek using fruit gum, applied in both clinical and nursing home settings. Methods: Seventy participants aged 18 to 99 years from dental clinics and nursing homes were included. All participants received a standardized dental examination (reference standard) and performed the MDA and CHEW tests. Sensitivity, specificity, and AUC values were calculated using ROC analysis. Participants rated both tests in terms of taste, consistency, comprehensibility, required time, and subjective chewing sensation. Acceptance was analyzed across age groups and prosthesis types. Results: Both chewing efficiency tests showed good agreement with the clinical reference standard. The AUC was 0.72 for the MDA and 0.78 for the CHEW test (p = 0.192). Sensitivity was higher for the CHEW test (100%) compared to the MDA (83.3%), while the MDA demonstrated slightly higher specificity (59.6% vs. 55.8%). Age significantly influenced both diagnostic outcomes and test acceptance (p < 0.05). Younger participants (<70 years) were more often correctly classified as healthy and tended to prefer the MDA, whereas older participants (≥70 years) preferred the CHEW test, primarily due to taste. Misclassifications occurred most frequently among participants with complete dentures. Conclusions: Both the digital MDA and the CHEW chewing test demonstrated good diagnostic performance in identifying treatment need. Acceptance varied significantly with age, suggesting that test selection may be optimized based on patient characteristics. These simple and rapid assessments may support early detection of dental treatment needs in clinical and nursing home settings. Full article

The market for remanufactured products made from marine plastic waste is expanding rapidly, but the recycling rate of this waste remains strikingly low. This disconnect forces conventional plastic recycling firms to make a consequential strategic choice: enter the marine plastic recycling supply segment by expanding to build market power or enter by competing as a specialized supplier. To examine this trade-off, this paper develops a two-period game-theoretic model that contrasts entry strategies and performance under monopolistic and competitive market structures. We derive and compare equilibrium pricing, quantities, and profits for the relevant supply chain participants in both settings and then characterize the conditions under which one entry mode dominates the other. The results indicate that neither the preferred entry strategy nor the profitability that follows is driven by a single parameter. Instead, outcomes are shaped by the joint effects of consumer tastes, remanufacturing costs, and the scale of capacity investment cost required for entry. When consumers show a stronger preference for conventional remanufactured products, a supplier pursuing monopolistic expansion can earn higher profits by offering a more flexible product portfolio. By contrast, when the cost of remanufacturing marine plastics and the associated capacity investment cost are relatively low, the environment favors a specialized, competitively oriented entry strategy. Profit allocation within the supply chain is also closely tied to remanufacturer costs: as these costs fall, suppliers are able to appropriate a larger share of total profits. Overall, the analysis provides a theoretical basis for entry decisions in the emerging marine plastic recycling industry and offers actionable guidance for firms facing different demand and cost conditions across market structures. Full article

Background/Objectives: Adolescence is pivotal for establishing dietary habits; however, school-based nutritional education remains focused on information dissemination, with minimal effects on behavior modification. Evidence from neuroscience, education, and food literacy indicates that attention, engagement, sensory experiences, and social contexts are integral to effective learning in nutrition education. This article conceptualizes a framework for adolescent food education beyond knowledge transmission, aiming to cultivate taste competence using the Mediterranean Diet as a pedagogical ecosystem. Methods: This study employed a conceptual methodology, utilizing interdisciplinary literature from food literacy, sensory education, developmental neuroscience, educational theory, and public health nutrition. It synthesizes empirical findings and theoretical models to develop the Teaching Autonomous Sensory Taste in the Mediterranean Diet (TASTE–MED) framework. Results: This study introduces taste competence as a multifaceted educational outcome, encompassing sensory, relational, cultural, and reflective dimensions. The TASTE–MED framework outlines how experiential, multisensory, and socially embedded learning processes can be implemented in schools, facilitated by the Mediterranean Diet, which provides a sensory-rich and culturally significant context. The educational implications are discussed in terms of curriculum design, teacher training, family involvement and digital tools. Conclusions: The TASTE–MED framework redefines food literacy as an embodied and socially situated competence rather than a cognitive construct. This framework provides a theoretical foundation for informing the design, evaluation, and research of future interventions, advocating for the transition from information-based nutrition education to competence-oriented food education during adolescence. Full article

Six different vinification treatments were applied to evaluate the effect of prefermentative ultrasound treatments on bioactive compounds and taste sensory attributes of autochthonous Croatian grape variety Malvazija istarska. Four of them were based on the application of a prefermentative ultrasound technique on cooled cryomacerated mash (at 10 °C for 24 h) as follows: ultrasound treatments of 70% amplitude for 80 min (US80-70%) and 160 min (US160-70%) and ultrasound treatments of 100% amplitude for the same durations as the previous (US80-100% and US160-100%). The research also included a control treatment C (wine produced using standard white winemaking technology without maceration) and a cryomaceration treatment lasting one day at 10 °C (CRIO). Phenolic compounds in wine were analyzed by HPLC-DAD-FLD, total phenolic content (TPC), antioxidant activity and color intensity by UV/VIS spectrophotometry, and sensory evaluation was performed using the QDA and 100-point O.I.V./U.I.O.E. methods. Ultrasound-treated wines exhibited the most pronounced increases in TPC, antioxidant activity and color intensity, as well as total hydroxycinnamic and hydroxybenzoic acids, flavonols, flavan-3-ols, stilbenes and the total HPLC phenolic concentration. All wines obtained after ultrasound treatments received the highest scores by both sensory methods, in the majority of sensory attributes, especially the US160-100% treatment. The differences observed between treatments indicate that both ultrasound amplitude and duration play a key role in optimizing extraction of phenolic compounds and improving sensory attributes of the wine. The results indicate that ultrasound treatments significantly enhance the bioactive composition and sensory profile of Malvazija istarska wines, highlighting their nutritional, health-related, and market potential. Full article

Background: Childhood obesity is on the rise in South Africa and adolescents spend a substantial amount of time in the school food environment (SFE), which plays a role in shaping their food choices and provides a critical setting to improve diets. Objective: To investigate South African adolescent school-going learners’ knowledge and understanding of healthy and unhealthy foods and the drivers of their food choices in their (SFE). Design: Qualitative participatory research methods including workshops, photovoice and focus group discussions (FGDs). Setting: Two urban public high schools, one non-metropolitan and one metropolitan, in two separate provinces (Eastern Cape and Gauteng) in South Africa. Participants: Adolescents 14–18 years (n = 42). Results: Unhealthy ultra-processed foods (UPFs) were found to be rampant in the SFE, and healthy foods were scarce, limiting learners’ choices. Taste preference was a major driver of adolescent food choices as were satiety, value for money, affordability, convenience, visual appeal and seeming “cool or “rich” by purchasing branded franchise fast foods. Learners had some general nutrition knowledge, but this did not translate into healthy food choices. Banning unhealthy foods in the SFE and providing affordable and satiating healthy foods were proposed as solutions. Conclusions: UPFs such as packaged foods and fast food were considered tasty but unhealthy, yet were preferred. Interventions are needed to promote healthy diets by changing the SFE, and eventually adolescent food choices. This will require government regulation banning the sale of unhealthy food and beverages (F&Bs) in the SFE and subsidising healthy satiating foods to change dietary behaviour. Full article