Search Results (11,597)

Background/Objectives: Human adenovirus 55 (HAdV55) is a notable pathogen causing community-acquired pneumonia; outbreaks occur frequently in military camps, hospitals, and schools, thereby posing a threat to public health security. This study aimed to develop a method for detecting HAdV55 nucleic acid by targeting the conserved region of the Hexon gene. The sequence was amplified using enzymatic recombination isothermal amplification (ERA) technology, in conjunction with CRISPR-Cas12a technology, to enhance the amplification signal. Methods: Optimized primer and crRNA sequences were selected through ERA isothermal amplification testing. The ERA-CRISPR/Cas12a detection method was completed within 30 min at a constant temperature of 42 °C. Results: Sensitivity was assessed by detecting standard plasmids and live strains at various dilution concentrations. The detection limits were determined to be 9 copies/reaction for standard plasmids and 2.5 copies/reaction for cultured HAdV55 strains. Specificity tests were conducted on positive samples for five common respiratory pathogens and five other adenovirus subtypes, all of which showed no cross-reactivity. Conclusions: A rapid ERA-CRISPR/Cas12a nucleic acid detection method for HAdV55 has been successfully developed, demonstrating high sensitivity and specificity without the need for expensive or complex instruments. This method holds promise for on-site pathogen screening and detection. Full article

Fiber-reinforced polymer (FRP) composites have become a popular solution for upgrading reinforced concrete (RC) structures due to their corrosion resistance, high strength-to-weight ratio, and speed of implementation. However, their organic resin binder has issues, including temperature sensitivity, poor performance in moist conditions, a high cost, and potential health risks. Additionally, reversing FRP repair can be difficult and may damage the original structure, posing a significant reversibility issue. A promising alternative to FRP is the fiber-reinforced cementitious matrix (FRCM), which replaces the organic resin with an inorganic cementitious mortar. This new class of composite uses a breathable textile instead of the tightly packed fibers in FRP. The present article provides a comprehensive assessment of the two composites (FRP and FRCM) used for the retrofitting of RC compression members, with the purpose of identifying existing knowledge gaps and outlining future research objectives. The materials used in different strengthening approaches using both FRP and FRCM have been identified, and their stress–strain characteristics under tensile load have been outlined. The study also explores techniques of implementation using the two materials. This study presents available studies comparing the utilization of FRCM composites with FRP for the axial retrofitting of RC compression members in both ambient and high-temperature conditions. Full article

Climate change is intensifying heat stress conditions in livestock systems, posing significant challenges to animal welfare, productivity, and food quality. This study aims to investigate the combined effects of seasonal heat stress and postpartum physiology on the milk fatty acid (FA) profile of Holstein dairy cows in Galicia, Spain. Forty milk samples were collected during winter and summer and at 1 week and 1 month postpartum. Fatty acid composition was analyzed via gas chromatography (GC-FID), and heat stress exposure was quantified using the temperature–humidity index (THI). Results revealed that heat stress significantly altered the milk lipid profile, with increased concentrations of short- and medium-chain fatty acids (SMCFA) such as C10:0 and C14:1 (n-5), and conjugated linoleic acids (CLAs), suggesting enhanced de novo lipogenesis and shifts in rumen fermentation. Conversely, unsaturated long-chain fatty acids (LCFAs), including cis-11 C18:1 and cis-13 C18:1, decreased with lactation progression and thermal exposure. Notably, certain LCFAs remained stable under combined seasonal and physiological stress, indicating potential metabolic regulation. These results show how milk composition is sensitive to environmental stress and emphasize the need for climate-resilient management to protect milk quality under global warming. Full article

The existing research on open-set recognition (OSR) and novel class discovery (NCD) has largely treated these tasks as independent fields. OSR aims to identify samples that do not belong to the training set classes, while NCD seeks to further classify such unseen, unlabeled samples into novel classes. However, there is a lack of a unified framework to automate both tasks systematically. In this paper, we propose a unified training framework to identify and categorize unseen samples. Specifically, we conduct a comprehensive evaluation of existing post hoc OSR methods and observe that their performance is highly sensitive to the temperature scaling factor. To address this, we introduce a distance-based evaluation method for OSR, which not only outperforms existing post hoc approaches but also integrates seamlessly with them to deliver enhanced performance. Furthermore, we developed a prototype-based classification head leveraging this distance metric, which facilitates compact feature representations for known classes and guides the clustering of unknown classes, thereby significantly enhancing the classification accuracy for novel classes. On the CUB-200-2011 dataset, our unified framework achieves a 0.95–6.12% improvement in AUROC scores on OSR benchmarks and a 3.19% increase in classification accuracy for novel classes. Extensive experiments and visualizations validate the effectiveness of the proposed approach. We believe that this unified framework will pave the way for automating the integration of OSR and NCD, offering a more efficient and systematic approach to addressing these tasks. Full article

Buildings in Indonesia’s tropical climate face significant barriers to energy efficiency due to high cooling loads and electricity intensity. Previous studies have primarily addressed technical optimization or policy frameworks, but few have provided an integrated and data-driven evaluation model for tropical conditions. This study develops an Internet of Things (IoT) and EDGE-based hybrid framework to support the transition toward Net-Zero-Energy Buildings (NZEBs) while maintaining occupant comfort. The research combines real-time IoT monitoring at the LLDIKTI Region XIII Office Building in Banda Aceh with simulation-based assessment using Excellence in Design for Greater Efficiencies (EDGE). Baseline energy performance was established from architectural data, historical electricity use, and live monitoring of HVAC systems, lighting, temperature, humidity, and CO₂ concentration. Intervention scenarios—including building envelope enhancement, lighting optimization, and adaptive HVAC control—were simulated and validated against empirical data. Results demonstrate that integrating IoT-driven control with passive design measures achieves up to 31.49% reduction in energy use intensity, along with 24.7% improvement in water efficiency and 22.3% material resource savings. These findings enhance indoor environmental quality and enable adaptive responses to user behavior. The study concludes that the proposed IoT–EDGE framework offers a replicable and context-sensitive pathway for achieving net-zero energy operations in tropical office buildings, with quantifiable environmental benefits that support sustainable public facility management in Indonesia. Full article

Wide-Bandgap (WBG) semiconductors—silicon carbide (SiC) and gallium nitride (GaN)— enable high-power-density conversion, but performance is limited by where heat is generated and how it is removed. This review links device-level loss mechanisms (conduction and switching, including output-capacitance hysteresis and dynamic on-resistance) to structure-driven hot spots within the ultra-thin (tens of nanometers) two-dimensional electron gas (2DEG) channel of GaN HEMTs and to thermal boundary resistance at layer interfaces. We compare wire-bondless package concepts—double-sided cooling, embedded packaging, and interleaved planar layouts—and survey system-level cooling that shortens the conduction path and raises heat-transfer coefficients. The impact on reliability is discussed using temperature-sensitive electrical parameters (e.g., on-state VDS, threshold voltage, drain leakage, di/dt, and gate current) for real-time junction-temperature estimation and compact electro-thermal RC models for remaining-useful-life prediction. Evidence from recent literature points to interface resistance in GaN-on-SiC as a primary bottleneck, while near-junction cooling and advanced packages are effective mitigations. We argue for integrated co-design—devices, packaging, electromagnetic interference (EMI)-aware layout, and cooling—together with interface engineering and health monitoring to deliver reliable, high-density WBG systems. Full article

Skin compatibility is a common issue and can often be worsened by certain ingredients in cosmetics. This is why developing well-balanced and -tolerated formulas is now an essential challenge. In this work we developed a cream rich in antioxidant, soothing, and moisturizing agents complying with concentration limits for sensitive skin. An initial optimization was carried out, and the best-performing formula was fully characterized to test its rheological properties under static or dynamic conditions and product safety. The formulation proved to be highly stable even under thermal stress, as shown by Turbiscan Lab analyses, which reported backscattering values ±2. Rheological tests also indicated a solid-like behavior with reduced viscosity at skin temperature of 32 °C, confirming the good spreadability of the cream. Finally, in vivo tests on healthy volunteers showed excellent safety results and good overall appreciation of the product. No changes in transepidermal water loss (7.9 ± 3.5 vs. 5.5 ± 0.4, p > 0.05), skin hydration (44.2 ± 18.6 vs. 50.5 ± 14.1, p > 0.05), or color were detected within 6 h from application, compared with baseline values. Moreover, volunteers highlighted the cream’s suitability for dry skin and expressed satisfaction with spreadability, a nourishing and hydrating sensation after application, and the absence of residues, consistently rating them ≥4 in the skin feeling questionnaire. These results are promising and support the potential use of the product on sensitive skin. Full article

The Yangtze sturgeon (Acipenser dabryanus) is designated as critically endangered in the IUCN Red List and is a first-class protected species in China. During the summer of 2024, it suffered lethal disease outbreaks. Comprehensive pathological and microbiological analyses were conducted to clarify the etiology. Clinically, infected sturgeon exhibited systemic manifestations including cutaneous ulcers, hemorrhagic septicemia, and diffuse necrosis in liver, kidney and heart tissues. Histopathologically, infected sturgeon showed liver hepatocyte vacuolation/necrosis, renal glomerular atrophy, and cardiac epicardial thickening with lymphocyte/eosinophil infiltration; Gram staining revealed co-localized Gram-positive/negative bacteria in lesions, and TEM identified diverse bacterial morphotypes. Through isolation and molecular identification, four bacterial pathogens were characterized: Streptococcus iniae, Klebsiella pneumoniae, Edwardsiella tarda, and Bacillus cereus. Bacterial load detection revealed the presence of these pathogens in lesion tissues. Antimicrobial susceptibility testing indicated multidrug resistance to florfenicol, tetracycline, and ampicillin (commonly used antibiotics in aquaculture), while high sensitivity to ceftazidime, ceftriaxone, and ciprofloxacin was observed. Thus, we infer that sustained high-temperature stress triggered bacterial co-infection is closely related to this large-scale death incident. This is the first evidence of polymicrobial infection in the Yangtze sturgeon, emphasizing the significance of shifting from a single-pathogen perspective to a multi-pathogen framework, and highlighting the urgency of implementing ecological prevention strategies for this species. Full article

The miniaturization of ocean parameter monitors is critical for environmental monitoring and oceanographic research. In recent years, rapid developments in data processing, artificial intelligence, micro-nano manufacturing and advanced materials have significantly improved sensing accuracy while reducing device size. The detection of key ocean parameters such as temperature, salinity, pressure, dissolved oxygen (DO), pH, nutrients, chlorophyll and so on is facilitated by these innovations. Among these emerging technologies, microfluidics and optofluidics have attracted large attention in the fields of biomedicine and environmental monitoring. These platforms have the advantages of high sensitivity, low power consumption and easy integration. Real-time and on-site monitoring can be achieved by them. Optofluidic technology shows great prospects for ocean sensing applications. Recent advances in optofluidic ocean sensors for the measurement of chemical parameters and their future potential are highlighted in this review. Ultimately, it presents the key role of optofluidic systems in realizing compact high-performance ocean parameter sensors. This plays an important role in paving the way for their integration into micro robots and the fourth generation of submersibles based on live fish. Full article

In the present work, a new analytical model of polytropic flow in constant-diameter pipelines is developed to accurately describe the flow of compressible gases, including natural gas and hydrogen, explicitly accounting for heat exchange between the fluid and the environment. In contrast to conventional models that assume isothermal or adiabatic conditions, the proposed model simultaneously accounts for variations in pressure, temperature, density, and entropy, i.e., it is based on a realistic polytropic gas flow formulation. A system of differential equations is established, incorporating the momentum, continuity, energy, and state equations of the gas. An implicit closed-form solution for the specific volume along the pipeline axis is then derived. The model is universal and allows the derivation of special cases such as adiabatic, isothermal, and isentropic flows. Numerical simulations demonstrate the influence of heat flow on the variation in specific volume, highlighting the critical role of heat exchange under real conditions for the optimization and design of energy systems. It is shown that achieving isentropic flow would require the continuous removal of frictional heat, which is not practically feasible. The proposed model therefore provides a clear, reproducible, and easily visualized framework for analyzing gas flows in pipelines, offering valuable support for engineering design and education. In addition, a unified sensitivity analysis of the analytical solutions has been developed, enabling systematic evaluation of parameter influence across the subsonic, near-critical, and heated flow regimes. Full article

Effective thermal management is essential in metal additive manufacturing to ensure process stability and desirable material properties. Directed energy deposition using a laser beam and wire (DED-LB/w) enables the production of large, high-performance components but remains sensitive to adverse thermal effects during multi-layer deposition due to heat accumulation. While prior studies have investigated interlayer temperature control and substrate preheating in DED modalities, including laser-powder and arc-based systems, the influence of substrate preheating in DED-LB/w has not been thoroughly examined. This study employs substrate preheating to simulate heat accumulation and assess its effects on melt pool geometry, wire–melt pool interaction, and the microstructural evolution of Alloy 718. Experimental results demonstrate that increased substrate temperatures lead to a gradual expansion of the melt pool, with a notable transition occurring beyond 400 °C. Microstructural analysis reveals that elevated preheat temperatures promote coarser secondary dendrite arm spacing and the development of wider columnar grains. Moreover, Nb-rich secondary phases, including the Laves phase, exhibit increased size but relatively unchanged area fractions. Observations from electrical conductance measurements and coaxial visual imaging show that preheat temperature significantly affects the process dynamics and microstructural evolution, providing a basis for advanced process control strategies. Full article

As high-energy-consumption underground public space, subway stations are responsible for a particularly significant proportion of air-conditioning energy use, especially during the cooling season, making the investigation of cooling-load characteristics highly important. However, the determination of independent influencing factors in different situations has not yet reached a consensus, and the role of interaction effects is lacking, which hinders the development of energy-saving strategies. For this purpose, this study proposes a sensitivity analysis framework based on 10 typical influencing factors from thermal parameters, meteorological parameters, internal heat disturbances, and indoor environmental setpoints. An input set was generated by integrating equal-step parameter discretization and Saltelli quasi-MonteCarlo sampling. A database containing 11,264 samples was constructed through an EnergyPlus–Python co-simulation method. Based on the Sobol global sensitivity analysis, the key influencing factors of subway station cooling load were identified and quantified, and the impact of these 10 factors was systematically analyzed. Results show that occupant density (S_i^T = 0.5605) and fresh air volume (S_i^T = 0.4546) are the dominant factors, contributing more than 50% of the load variance. In contrast, the characteristics of an underground structure significantly weaken the influence of the building-envelope heat transfer coefficient (S_i^T = 0.1482) and soil temperature (S_i^T = 0.0884). Furthermore, five groups of strong interaction effects were identified in this study, including occupant density–fresh air volume (S_ij = 0.1094), revealing a nonlinear load response mechanism driven by multi-parameter coupling. This research provides a theoretical foundation and quantitative tool for the refined design and optimized dynamic coupled operation of underground transportation hubs. Full article

Water loss during storage is a major cause of postharvest quality deterioration in cucumber, yet existing methods to monitor hydration are often destructive or require expensive instrumentation. We developed a low-cost, non-destructive approach for estimating fruit relative water content (RWC) using visible-light color imaging combined with an ensemble machine-learning model (Random Forest). A total of 1200 fruits were greenhouse-grown, harvested at market maturity, and equally divided between optimal and ambient storage temperature (10 and 25 °C, respectively). Digital images were acquired at harvest and at 7 d intervals during storage, and color parameters from four standard color systems (RGB, CMYK, CIELAB, HSV) were extracted separately for the neck, mid, and blossom regions as well as for the whole fruit. During storage, fruit RWC decreased from 100% (fully hydrated condition) to 15.3%, providing a broad dynamic range for assessing color–hydration relationships. Among the 16 color features evaluated, the mean cyan component (μC) of the CMYK space showed the strongest relationship with measured RWC (R² up to 0.70 for whole-fruit averages), reflecting the cyan region’s heightened sensitivity to dehydration-induced changes in pigments, cuticle properties and surface scattering. The Random Forest regression model trained on these features achieved a higher predictive accuracy (R² = 0.89). Predictive accuracy was also consistently higher when μC was calculated over the entire fruit surface rather than for individual anatomical regions, indicating that whole-fruit color information provides a more robust hydration signal than region-specific measurements. Our findings demonstrate that simple visible-range imaging coupled with ensemble learning can provide a cost-effective, non-invasive tool for monitoring postharvest hydration of cucumber fruit, with direct applications in quality control, shelf-life prediction and waste reduction across the fresh-produce supply chain. Full article

In the context of intensifying climate change, it is particularly important to assess the transformation of spring floods as a key phase of the hydrological regime of rivers. This study provides a comprehensive analysis of the characteristics of maximum runoff in the Zhaiyk–Caspian basin for the modern period and projected changes for 2030, 2040, and 2050 based on CMIP6 climate scenarios (SSP3-7.0 and SSP5-8.5). Analysis of observations at 34 hydrological stations showed a reduction in spring runoff by up to 35%, a decrease in the duration of high water and a reduction in maximum water discharge on some rivers by up to 45%. It has been established that those rising temperatures, more frequent thaws, and reduced autumn moisture lead to lower maximum water discharge and a redistribution of the seasonal flow regime. Scenario projections revealed significant spatial heterogeneity: some rivers are expected to experience an increase in maximum discharge of up to 72%, while others will see a steady decline in maximum discharge of up to 35%. The results obtained indicate the need to transition to an adaptive water management system focused on the regional characteristics of river basins and the sensitivity of small- and medium-sized watercourses to climate change. Full article

Advancements in flexible, low-cost, and recyclable alternatives to transparent conductive oxides (TCOs) are critical challenges in the sustainability of third-generation solar cells. This work introduces a copper mesh-based transparent electrode for dye-sensitized solar cells, replacing conventional fluorine doped-tin oxide (FTO)-coated glass to simultaneously reduce spectral reflection losses, enhance mechanical flexibility, and enable material recyclability. Titanium dioxide (TiO₂) photoanodes were synthesized and directly deposited onto the mesh via a single-step, low-energy ball milling process, which eliminates TiO₂ paste preparation and high-temperature annealing while reducing fabrication time from over three hours to 30 min. Structural and surface analyses confirmed the deposition of high-purity anatase-phase TiO₂ with strong adhesion to the mesh branches, enabling improved dye loading and electron injection pathways. Optical studies revealed higher visible light absorption for the copper mesh compared to FTO in the visible range, further enhanced upon TiO₂ and Ru-based dye deposition. Electrochemical measurements showed that TiO₂/Cu mesh electrodes exhibited significantly higher photocurrent densities and faster photo response rates than bare Cu mesh, with dye-sensitized Cu mesh achieving the lowest charge transfer resistance in impedance analysis. Techno–economic and sustainability assessments revealed a decrease of 7.8% in cost and 82% in CO₂ emissions associated with the fabrication of electrodes as compared to conventional TCO electrodes. The synergy between high conductivity, transparency, mechanical durability, and a scalable, recyclable fabrication route positions this architecture as a strong candidate for next-generation dye-sensitized solar modules that are both flexible and sustainable. Full article