Point-of-Care Testing (POCT) for Infectious Diseases

A special issue of Diagnostics (ISSN 2075-4418). This special issue belongs to the section "Diagnostic Microbiology and Infectious Disease".

Deadline for manuscript submissions: 30 April 2026 | Viewed by 511

Special Issue Editor


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Guest Editor
1. Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, China
2. Beijing Key Laboratory of Environment and Aging, Beijing, China
Interests: microfluidic chip; biosensor; nanomaterial; point-of-care test; pathogen; infectious disease; respiratory disease; drug resistance; organ/organoid on a chip; lab on a chip

Special Issue Information

Dear Colleagues,

This Special Issue highlights cutting-edge advances in point-of-care testing (POCT) for infectious diseases, presenting the latest global research and practical cases. With millions dying annually from infectious diseases worldwide, POCT—featuring portability, simplicity, rapidity, and cost-effectiveness—greatly boosts treatment efficiency and resource utilization, becoming vital for primary care and outbreak response.

It explores the on-site early detection and clinical value of technologies (molecular diagnostics, immunochromatography, microfluidic chips, nanomaterials, and biosensors) in respiratory, gastrointestinal, and bloodstream infections, among others. Contributions integrating artificial intelligence (AI), wearable real-time monitoring, automated data transmission, and cloud analysis are encouraged. This Special Issue provides technical support for public health emergencies, hierarchical healthcare, and home diagnostics, aiding a faster, more accessible infectious disease control system.

Prof. Dr. Rongzhang Hao
Guest Editor

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Keywords

  • point-of-care testing (POCT)
  • microfluidic chip and biosensor
  • ultrafast detection techniques
  • AI-integrated testing systems
  • infectious disease
  • pathogen

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Published Papers (1 paper)

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Research

13 pages, 1754 KB  
Article
An ERA-CRISPR/Cas12a Method for Highly Sensitive Detection of Human Adenovirus Type 55
by Letian Zhang, Zhenghan Luo, Taiwu Wang, Yifang Han, Fuqiang Ye, Chunhui Wang, Yue Chen and Jinhai Zhang
Diagnostics 2025, 15(21), 2725; https://doi.org/10.3390/diagnostics15212725 - 27 Oct 2025
Viewed by 243
Abstract
Background/Objectives: Human adenovirus 55 (HAdV55) is a notable pathogen causing community-acquired pneumonia; outbreaks occur frequently in military camps, hospitals, and schools, thereby posing a threat to public health security. This study aimed to develop a method for detecting HAdV55 nucleic acid by targeting [...] Read more.
Background/Objectives: Human adenovirus 55 (HAdV55) is a notable pathogen causing community-acquired pneumonia; outbreaks occur frequently in military camps, hospitals, and schools, thereby posing a threat to public health security. This study aimed to develop a method for detecting HAdV55 nucleic acid by targeting the conserved region of the Hexon gene. The sequence was amplified using enzymatic recombination isothermal amplification (ERA) technology, in conjunction with CRISPR-Cas12a technology, to enhance the amplification signal. Methods: Optimized primer and crRNA sequences were selected through ERA isothermal amplification testing. The ERA-CRISPR/Cas12a detection method was completed within 30 min at a constant temperature of 42 °C. Results: Sensitivity was assessed by detecting standard plasmids and live strains at various dilution concentrations. The detection limits were determined to be 9 copies/reaction for standard plasmids and 2.5 copies/reaction for cultured HAdV55 strains. Specificity tests were conducted on positive samples for five common respiratory pathogens and five other adenovirus subtypes, all of which showed no cross-reactivity. Conclusions: A rapid ERA-CRISPR/Cas12a nucleic acid detection method for HAdV55 has been successfully developed, demonstrating high sensitivity and specificity without the need for expensive or complex instruments. This method holds promise for on-site pathogen screening and detection. Full article
(This article belongs to the Special Issue Point-of-Care Testing (POCT) for Infectious Diseases)
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