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21 pages, 3358 KB  
Article
Wave-Induced Loads and Fatigue Life of Small Vessels Under Complex Sea States
by Pasqualino Corigliano, Claudio Alacqua, Davide Crisafulli and Giulia Palomba
J. Mar. Sci. Eng. 2025, 13(10), 1920; https://doi.org/10.3390/jmse13101920 (registering DOI) - 6 Oct 2025
Abstract
The Strait of Messina poses unique challenges for small vessels due to strong currents and complex wave conditions, which critically affect structural integrity and operational safety. This study proposes an integrated methodology that combines seakeeping analysis, a comparison with classification society rules, and [...] Read more.
The Strait of Messina poses unique challenges for small vessels due to strong currents and complex wave conditions, which critically affect structural integrity and operational safety. This study proposes an integrated methodology that combines seakeeping analysis, a comparison with classification society rules, and fatigue life assessment within a unified and computationally efficient framework. A panel-based approach was used to compute vessel motions and vertical bending moments at different speeds and wave directions. Hydrodynamic loads derived from Response Amplitude Operators (RAOs) were compared with regulatory limits and applied to fatigue analysis. A further innovative aspect is the use of high-resolution bathymetric data from the Strait of Messina, enabling a realistic representation of local currents and sea states and providing a more accurate assessment than studies based on idealized conditions. The results show that forward speed amplifies bending moments, reducing safe wave heights from 2 m at rest to about 0.5 m at 16 knots. Fatigue analysis indicates that aluminum hulls are highly vulnerable to 2–3 m waves, while steel and titanium show no significant damage. The proposed workflow is transferable to other vessel types and supports safer design and operation. The case study of the Strait of Messina, the busiest and most challenging maritime corridor in Italy, confirms the validity and practical importance of the approach. By combining hydrodynamic and structural analyses into a single workflow, this study establishes the foundation for predictive maintenance and real-time structural health monitoring, with significant implications for navigation safety in complex sea environments. Full article
(This article belongs to the Special Issue Advanced Studies in Marine Mechanical and Naval Engineering)
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11 pages, 703 KB  
Article
A Novel Approach to Day-Ahead Forecasting of Battery Discharge Profiles in Grid Applications Using Historical Daily
by Marek Bobček, Róbert Štefko, Július Šimčák and Zsolt Čonka
Batteries 2025, 11(10), 370; https://doi.org/10.3390/batteries11100370 (registering DOI) - 6 Oct 2025
Abstract
This paper presents a day-ahead forecasting approach for discharge profiles of a 0.5 MW battery energy storage system connected to the power grid, utilizing historical daily discharge profiles collected over one year to capture key operational patterns and variability. Two forecasting techniques are [...] Read more.
This paper presents a day-ahead forecasting approach for discharge profiles of a 0.5 MW battery energy storage system connected to the power grid, utilizing historical daily discharge profiles collected over one year to capture key operational patterns and variability. Two forecasting techniques are employed: a Kalman filter for dynamic state estimation and Holt’s exponential smoothing method enhanced with adaptive alpha to capture trend changes more responsively. These methods are applied to generate next-day discharge forecasts, aiming to support better battery scheduling, improve grid interaction, and enhance overall energy management. The accuracy and robustness of the forecasts are evaluated against real operational data. The results confirm that combining model-based and statistical techniques offers a reliable and flexible solution for short-term battery discharge prediction in real-world grid applications. Full article
(This article belongs to the Special Issue Towards a Smarter Battery Management System: 3rd Edition)
22 pages, 1439 KB  
Review
Unlocking the Secrets of the Endometrium: Stem Cells, Niches and Modern Methodologies
by Lijun Huang, Miaoxian Ou, Dunjin Chen and Shuang Zhang
Biomedicines 2025, 13(10), 2435; https://doi.org/10.3390/biomedicines13102435 (registering DOI) - 6 Oct 2025
Abstract
The endometrium is a highly dynamic tissue central to female reproductive function, undergoing nearly 500 cycles of proliferation, differentiation, shedding, and regeneration throughout a woman’s reproductive life. This remarkable regenerative capacity is driven by a reservoir of endometrial stem/progenitor cells (ESCs), which are [...] Read more.
The endometrium is a highly dynamic tissue central to female reproductive function, undergoing nearly 500 cycles of proliferation, differentiation, shedding, and regeneration throughout a woman’s reproductive life. This remarkable regenerative capacity is driven by a reservoir of endometrial stem/progenitor cells (ESCs), which are crucial for maintaining tissue homeostasis. Dysregulation of these cells is linked to a variety of clinical disorders, including menstrual abnormalities, infertility, recurrent pregnancy loss, and serious gynecological conditions such as endometriosis and endometrial cancer. Recent advancements in organoid technology and lineage-tracing models have provided insights into the complex cellular hierarchy that underlies endometrial regeneration and differentiation. This review highlights the latest breakthroughs in endometrial stem cell biology, focusing particularly on 3D in vitro platforms that replicate endometrial physiology and disease states. By integrating these cutting-edge approaches, we aim to offer new perspectives on the pathogenesis of endometrial disorders and establish a comprehensive framework for developing precision regenerative therapies. Full article
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18 pages, 2798 KB  
Article
Exploring Low Energy Excitations in the d5 Iridate Double Perovskites La2BIrO6 (B = Zn, Mg)
by Abhisek Bandyopadhyay, Dheeraj Kumar Pandey, Carlo Meneghini, Anna Efimenko, Marco Moretti Sala and Sugata Ray
Condens. Matter 2025, 10(4), 53; https://doi.org/10.3390/condmat10040053 (registering DOI) - 6 Oct 2025
Abstract
We experimentally investigate the structural, magnetic, transport, and electronic properties of two d5 iridate double perovskite materials La2BIrO6 (B = Mg, Zn). Notably, despite similar crystallographic structure, the two compounds show distinctly different magnetic behaviors. The M [...] Read more.
We experimentally investigate the structural, magnetic, transport, and electronic properties of two d5 iridate double perovskite materials La2BIrO6 (B = Mg, Zn). Notably, despite similar crystallographic structure, the two compounds show distinctly different magnetic behaviors. The M = Mg compound shows an antiferromagnetic-like linear field-dependent isothermal magnetization below its transition temperature, whereas the M = Zn counterpart displays a clear hysteresis loop followed by a noticeable coercive field, indicative of ferromagnetic components arising from a non-collinear Ir spin arrangement. The local structure studies authenticate perceptible M/Ir antisite disorder in both systems, which complicates the magnetic exchange interaction scenario by introducing Ir-O-Ir superexchange pathways in addition to the nominal Ir-O-B-O-Ir super-superexchange interactions expected for an ideally ordered structure. While spin–orbit coupling (SOC) plays a crucial role in establishing insulating behavior for both these compounds, the rotational and tilting distortions of the IrO6 (and MO6) octahedral units further lift the ideal cubic symmetry. Finally, by measuring the Ir-L3 edge resonant inelastic X-ray scattering (RIXS) spectra for both the compounds, giving evidence of spin–orbit-derived low-energy inter-J-state (intra t2g) transitions (below ~1 eV), the charge transfer (O 2p → Ir 5d), and the crystal field (Ir t2geg) excitations, we put forward a qualitative argument for the interplay among effective SOC, non-cubic crystal field, and intersite hopping in these two compounds. Full article
(This article belongs to the Section Quantum Materials)
15 pages, 574 KB  
Review
Guide to the Effects of Vibration on Health—Quantitative or Qualitative Occupational Health and Safety Prevention Guidance? A Scoping Review
by Eckardt Johanning and Alice Turcot
Vibration 2025, 8(4), 63; https://doi.org/10.3390/vibration8040063 - 6 Oct 2025
Abstract
This systematic review examined the health risk assessment methods of studies of whole-body vibration exposure from occupational vehicles or machines utilizing the International Standard ISO 2631-1 (1997) and/or the European Machine Directive 2002/44. This review found inconsistent reporting of measurement parameters in studies [...] Read more.
This systematic review examined the health risk assessment methods of studies of whole-body vibration exposure from occupational vehicles or machines utilizing the International Standard ISO 2631-1 (1997) and/or the European Machine Directive 2002/44. This review found inconsistent reporting of measurement parameters in studies on whole-body vibration (WBV) exposure. Although many authors treat the ISO 2631-1 HGCZ as a medical health standard with defined threshold levels, the epidemiological evidence for these limits is unclear. Similarly, the EU Directive offers more comprehensive risk management guidance, but the numeric limits are equal without supporting scientific evidence. Both guidelines likely represent the prevailing societal and interdisciplinary consensus at the time. Authors note discrepancies between international and national standards and adverse WBV exposure outcomes are reported below given boundaries. Future publications should report all relevant parameters from ISO 2631-1 and clearly state study limitations, exercising caution when applying ISO 2631-1 HGCZ in health and safety assessments and considering different susceptibility of diverse populations. We advise reducing WBV exposure to the lowest technically feasible limits wherever possible and applying the precautionary principle with attention to individual differences, instead of depending solely on numeric limits. Full article
41 pages, 200492 KB  
Article
A Context-Adaptive Hyperspectral Sensor and Perception Management Architecture for Airborne Anomaly Detection
by Linda Eckel and Peter Stütz
Sensors 2025, 25(19), 6199; https://doi.org/10.3390/s25196199 - 6 Oct 2025
Abstract
The deployment of airborne hyperspectral sensors has expanded rapidly, driven by their ability to capture spectral information beyond the visual range and to reveal objects that remain obscured in conventional imaging. In scenarios where prior target signatures are unavailable, anomaly detection provides an [...] Read more.
The deployment of airborne hyperspectral sensors has expanded rapidly, driven by their ability to capture spectral information beyond the visual range and to reveal objects that remain obscured in conventional imaging. In scenarios where prior target signatures are unavailable, anomaly detection provides an effective alternative by identifying deviations from the spectral background. However, real-world reconnaissance and monitoring missions frequently take place in complex and dynamic environments, requiring anomaly detectors to demonstrate robustness and adaptability. These requirements have rarely been met in current research, as evaluations are still predominantly based on small, context-restricted datasets, offering only limited insights into detector performance under varying conditions. To address this gap, we propose a context-adaptive hyperspectral sensor and perception management (hSPM) architecture that integrates sensor context extraction, band selection, and detector management into a single adaptive processing pipeline. The architecture is systematically evaluated on a new, large-scale airborne hyperspectral dataset comprising more than 1100 annotated samples from two diverse test environments, which we publicly release to support future research. Comparative experiments against state-of-the-art anomaly detectors demonstrate that conventional methods often lack robustness and efficiency, while hSPM consistently achieves superior detection accuracy and faster processing. Depending on evaluation conditions, hSPM improves anomaly detection performance by 28–204% while reducing computation time by 70–99%. These results highlight the advantages of adaptive sensor processing architectures and underscore the importance of large, openly available datasets for advancing robust airborne hyperspectral anomaly detection. Full article
(This article belongs to the Section Sensing and Imaging)
24 pages, 38672 KB  
Article
RMTDepth: Retentive Vision Transformer for Enhanced Self-Supervised Monocular Depth Estimation from Oblique UAV Videos
by Xinrui Zeng, Bin Luo, Shuo Zhang, Wei Wang, Jun Liu and Xin Su
Remote Sens. 2025, 17(19), 3372; https://doi.org/10.3390/rs17193372 - 6 Oct 2025
Abstract
Self-supervised monocular depth estimation from oblique UAV videos is crucial for enabling autonomous navigation and large-scale mapping. However, existing self-supervised monocular depth estimation methods face key challenges in UAV oblique video scenarios: depth discontinuity from geometric distortion under complex viewing angles, and spatial [...] Read more.
Self-supervised monocular depth estimation from oblique UAV videos is crucial for enabling autonomous navigation and large-scale mapping. However, existing self-supervised monocular depth estimation methods face key challenges in UAV oblique video scenarios: depth discontinuity from geometric distortion under complex viewing angles, and spatial ambiguity in weakly textured regions. These challenges highlight the need for models that combine global reasoning with geometric awareness. Accordingly, we propose RMTDepth, a self-supervised monocular depth estimation framework for UAV imagery. RMTDepth integrates an enhanced Retentive Vision Transformer (RMT) backbone, introducing explicit spatial priors via a Manhattan distance-driven spatial decay matrix for efficient long-range geometric modeling, and embeds a neural window fully-connected CRF (NeW CRFs) module in the decoder to refine depth edges by optimizing pairwise relationships within local windows. To mitigate noise in COLMAP-generated depth for real-world UAV datasets, we constructed a high-fidelity UE4/AirSim simulation environment, which generated a large-scale precise depth dataset (UAV SIM Dataset) to validate robustness. Comprehensive experiments against seven state-of-the-art methods across UAVID Germany, UAVID China, and UAV SIM datasets demonstrate that our model achieves SOTA performance in most scenarios. Full article
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26 pages, 1180 KB  
Article
Adaptive Constraint-Boundary Learning-Based Two-Stage Dual-Population Evolutionary Algorithm
by Xinran Xiu, Fu Yu, Hongzhou Wang and Yiming Song
Mathematics 2025, 13(19), 3206; https://doi.org/10.3390/math13193206 - 6 Oct 2025
Abstract
In recent years, numerous constrained multi-objective evolutionary algorithms (CMOEAs) have been proposed to tackle constrained multi-objective optimization problems (CMOPs). However, most of them still struggle to achieve a good balance among convergence, diversity, and feasibility. To address this issue, we develop an adaptive [...] Read more.
In recent years, numerous constrained multi-objective evolutionary algorithms (CMOEAs) have been proposed to tackle constrained multi-objective optimization problems (CMOPs). However, most of them still struggle to achieve a good balance among convergence, diversity, and feasibility. To address this issue, we develop an adaptive constraint-boundary learning-based two-stage dual-population evolutionary algorithm for CMOPs, referred to as CL-TDEA. The evolutionary process of CL-TDEA is divided into two stages. In the first stage, two populations cooperate weakly through environmental selection to enhance the exploration ability of CL-TDEA under constraints. In particular, the auxiliary population employs an adaptive constraint-boundary learning mechanism to learn the constraint boundary, which in turn enables the main population to more effectively explore the constrained search space and cross infeasible regions. In the second stage, the cooperation between the two populations drives the search toward the complete constrained Pareto front (CPF) through mating selection. Here, the auxiliary population provides additional guidance to the main population, helping it escape locally feasible but suboptimal regions by means of the proposed cascaded multi-criteria hierarchical ranking strategy. Extensive experiments on 54 test problems from four benchmark suites and three real-world applications demonstrate that the proposed CL-TDEA exhibits superior performance and stronger competitiveness compared with several state-of-the-art methods. Full article
22 pages, 1327 KB  
Review
Unequal Horizons: Global North–South Disparities in Archaeological Earth Observation (2000–2025)
by Athos Agapiou
Remote Sens. 2025, 17(19), 3371; https://doi.org/10.3390/rs17193371 - 6 Oct 2025
Abstract
This systematic review analyzes 4359 archaeologically relevant publications spanning 25 years to examine global disparities in archaeological remote sensing research between Global North and Global South participation. This study reveals deep inequalities among these regions, with 72.1% of research output originating from Global [...] Read more.
This systematic review analyzes 4359 archaeologically relevant publications spanning 25 years to examine global disparities in archaeological remote sensing research between Global North and Global South participation. This study reveals deep inequalities among these regions, with 72.1% of research output originating from Global North-only institutions, despite these regions hosting less than half of UNESCO World Heritage Sites. The temporal analysis demonstrates exponential growth, with 47.2% of all research published in the last five years, indicating rapid technological advancement concentrated in well-resourced institutions. Sub-Saharan Africa produces only 0.6% of research output while hosting 9.4% of World Heritage Sites, highlighting a technology gap in heritage protection. The findings suggest an urgent need for coordinated interventions to address structural inequalities and promote technological fairness in global heritage preservation. The research employed bibliometric analysis of Scopus database records from four complementary search strategies, revealing that just three countries—Italy (20.3%), the United States (16.7%), and the United Kingdom (10.0%)—account for nearly half of all archaeological remote sensing research and applications worldwide. This study documents patterns that have profound implications for cultural heritage preservation and sustainable development in an increasingly digital world where advanced Earth observation technologies have become essential for effective heritage protection and archaeological research. Full article
19 pages, 7766 KB  
Article
Fabric Flattening with Dual-Arm Manipulator via Hybrid Imitation and Reinforcement Learning
by Youchun Ma, Fuyuki Tokuda, Akira Seino, Akinari Kobayashi, Mitsuhiro Hayashibe and Kazuhiro Kosuge
Machines 2025, 13(10), 923; https://doi.org/10.3390/machines13100923 - 6 Oct 2025
Abstract
Fabric flattening is a critical pre-processing step for automated garment manufacturing. Most existing approaches employ single-arm robotic systems that act at a single contact point. Due to the nonlinear and deformable dynamics of fabric, such systems often require multiple actions to achieve a [...] Read more.
Fabric flattening is a critical pre-processing step for automated garment manufacturing. Most existing approaches employ single-arm robotic systems that act at a single contact point. Due to the nonlinear and deformable dynamics of fabric, such systems often require multiple actions to achieve a fully flattened state. This study introduces a dual-arm fabric-flattening method based on a cascaded Proposal–Action network with a hybrid training framework. The PA network is first trained through imitation learning from human demonstrations and is subsequently refined through reinforcement learning with real-world flattening feedback. Experimental results demonstrate that the hybrid training framework substantially improves the overall flattening success rate compared with a policy trained only on human demonstrations. The success rate for a single flattening operation increases from 74% to 94%, while the overall success rate improves from 82% to 100% after two rounds of training. Furthermore, the learned policy, trained exclusively on baseline fabric, generalizes effectively to fabrics with varying thicknesses and stiffnesses. The approach reduces the number of required flattening actions while maintaining a high success rate, thereby enhancing both efficiency and practicality in automated garment manufacturing. Full article
23 pages, 11972 KB  
Article
The Variability in the Thermophysical Properties of Soils for Sustainability of the Industrial-Affected Zone of the Siberian Arctic
by Tatiana V. Ponomareva, Kirill Yu. Litvintsev, Konstantin A. Finnikov, Nikita D. Yakimov, Georgii E. Ponomarev and Evgenii I. Ponomarev
Sustainability 2025, 17(19), 8892; https://doi.org/10.3390/su17198892 - 6 Oct 2025
Abstract
The sustainability of Arctic ecosystems that are extremely vulnerable is contingent upon the state of cryosoils. Understanding the principles of ecosystem stability in permafrost conditions, particularly under external natural or human-induced influences, necessitates an examination of the thermal and moisture regimes of the [...] Read more.
The sustainability of Arctic ecosystems that are extremely vulnerable is contingent upon the state of cryosoils. Understanding the principles of ecosystem stability in permafrost conditions, particularly under external natural or human-induced influences, necessitates an examination of the thermal and moisture regimes of the seasonally thawed soil layer. The study concentrated on the variability in the soil’s thermophysical properties in Central Siberia’s permafrost zone (the northern part of Krasnoyarsk Region, Taimyr, Russia). In the industrially affected area of interest, we evaluated and contrasted the differences in the thermophysical properties of soils between two opposing types of landscapes. On the one hand, these are soils that are characteristic of the natural landscape of flat shrub tundra, with a well-developed moss–lichen cover. An alternative is the soils in the landscape, which have exhibited significant degradation in the vegetation cover due to both natural and human-induced factors. The heat-insulating properties of background areas are controlled by the layer of moss and shrubs, while its disturbance determines the excessive heating of the soil at depth. In comparison to the background soil characteristics, degradation of on-ground vegetation causes the active layer depth of the soils to double and the temperature gradient to decrease. With respect to depth, we examine the changes in soil temperature and heat flow dynamics (q, W/m2). The ranges of thermal conductivity (λ, W/(m∙K)) were assessed using field-measured temperature profiles and heat flux values in the soil layers. The background soil was discovered to have lower thermal conductivity values, which are typical of organic matter, in comparison to the soil of the transformed landscape. Thermal diffusivity coefficients for soil layers were calculated using long-term temperature monitoring data. It is shown that it is possible to use an adjusted model of the thermal conductivity coefficient to reconstruct the dynamics of moisture content from temperature dynamics data. A satisfactory agreement is shown when the estimated (Wcalc, %) and observed (Wexp, %) moisture content values in the soil layer are compared. The findings will be employed to regulate the effects on landscapes in order to implement sustainable nature management in the region, thereby preventing the significant degradation of ecosystems and the concomitant risks to human well-being. Full article
(This article belongs to the Special Issue Land Use Strategies for Sustainable Development)
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16 pages, 1725 KB  
Article
A DAG-Based Offloading Strategy with Dynamic Parallel Factor Adjustment for Edge Computing in IoV
by Wenyang Guan, Qi Zheng, Xiaoqin Lian and Chao Gao
Sensors 2025, 25(19), 6198; https://doi.org/10.3390/s25196198 - 6 Oct 2025
Abstract
With the rapid development of Internet of Vehicles (IoV) technology, massive data are continuously integrated into intelligent transportation systems, making efficient computing resource allocation a critical challenge for enhancing network performance. Due to the dynamic and real-time characteristics of IoV tasks, existing static [...] Read more.
With the rapid development of Internet of Vehicles (IoV) technology, massive data are continuously integrated into intelligent transportation systems, making efficient computing resource allocation a critical challenge for enhancing network performance. Due to the dynamic and real-time characteristics of IoV tasks, existing static offloading strategies fail to effectively cope with the complexity caused by network fluctuations and vehicle mobility. To address this issue, this paper proposes a task offloading algorithm based on the dynamic adjustment of the parallel factor in directed acyclic graphs (DAG), referred to as Dynamic adjustment of Parallel Factor (DPF). By leveraging edge computing, the proposed algorithm adaptively adjusts the parallel factor according to the dependency relationships among subtasks in the DAG, thereby optimizing resource utilization and reducing task completion time. In addition, the algorithm continuously monitors network conditions and vehicle states to dynamically schedule and offload tasks according to real-time system requirements. Compared with traditional static strategies, the proposed method not only significantly reduces task delay but also improves task success rates and overall system efficiency. Extensive simulation experiments conducted under three different task load conditions demonstrate the superior performance of the proposed algorithm. In particular, under high-load scenarios, the DPF algorithm achieves markedly better task completion times and resource utilization compared to existing methods. Full article
(This article belongs to the Section Internet of Things)
27 pages, 8108 KB  
Review
A Review of Cross-Scale State Estimation Techniques for Power Batteries in Electric Vehicles: Evolution from Single-State to Multi-State Cooperative Estimation
by Ning Chen, Yihang Xie, Yuanhao Cheng, Huaiqing Wang, Yu Zhou, Xu Zhao, Jiayao Chen and Chunhua Yang
Energies 2025, 18(19), 5289; https://doi.org/10.3390/en18195289 - 6 Oct 2025
Abstract
As a critical technological foundation for electric vehicles, power battery state estimation primarily involves estimating the State of Charge (SOC), the State of Health (SOH) and the Remaining Useful Life (RUL). This paper systematically categorizes battery state estimation methods into three distinct generations, [...] Read more.
As a critical technological foundation for electric vehicles, power battery state estimation primarily involves estimating the State of Charge (SOC), the State of Health (SOH) and the Remaining Useful Life (RUL). This paper systematically categorizes battery state estimation methods into three distinct generations, tracing the evolutionary progression from single-state to multi-state cooperative estimation approaches. First-generation methods based on equivalent circuit models offer straightforward implementation but accumulate SOC-SOH estimation errors during battery aging, as they fail to account for the evolution of microscopic parameters such as solid electrolyte interphase film growth, lithium inventory loss, and electrode degradation. Second-generation data-driven approaches, which leverage big data and deep learning, can effectively model highly nonlinear relationships between measurements and battery states. However, they often suffer from poor physical interpretability and generalizability due to the “black-box” nature of deep learning. The emerging third-generation technology establishes transmission mechanisms from microscopic electrode interface parameters via electrochemical impedance spectroscopy to macroscopic SOC, SOH, and RUL states, forming a bidirectional closed-loop system integrating estimation, prediction, and optimization that demonstrates potential to enhance both full-operating-condition adaptability and estimation accuracy. This progress supports the development of high-reliability, long-lifetime electric vehicles. Full article
(This article belongs to the Section E: Electric Vehicles)
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21 pages, 1825 KB  
Article
IM-ZDD: A Feature-Enhanced Inverse Mapping Framework for Zero-Day Attack Detection in Internet of Vehicles
by Tao Chen, Gongyu Zhang and Bingfeng Xu
Sensors 2025, 25(19), 6197; https://doi.org/10.3390/s25196197 - 6 Oct 2025
Abstract
In the Internet of Vehicles (IoV), zero-day attacks pose a significant security threat. These attacks are characterized by unknown patterns and limited sample availability. Traditional anomaly detection methods often fail because they rely on oversimplified assumptions, hindering their ability to model complex normal [...] Read more.
In the Internet of Vehicles (IoV), zero-day attacks pose a significant security threat. These attacks are characterized by unknown patterns and limited sample availability. Traditional anomaly detection methods often fail because they rely on oversimplified assumptions, hindering their ability to model complex normal IoV behavior. This limitation results in low detection accuracy and high false alarm rates. To overcome these challenges, we propose a novel zero-day attack detection framework based on Feature-Enhanced Inverse Mapping (IM-ZDD). The framework introduces a two-stage process. In the first stage, a feature enhancement module mitigates data scarcity by employing an innovative multi-generator, multi-discriminator Conditional GAN (CGAN) with dynamic focusing loss to generate a large-scale, high-quality synthetic normal dataset characterized by sharply defined feature boundaries. In the second stage, a learning-based inverse mapping module is trained exclusively on this synthetic data. Through adversarial training, the module learns a precise inverse mapping function, thereby establishing a compact and expressive representation of normal behavior. During detection, samples that cannot be effectively mapped are identified as attacks. Experimental results on the F2MD platform show IM-ZDD achieves superior accuracy and a low false alarm rate, yielding an average AUC of 98.25% and F1-Score of 96.41%, surpassing state-of-the-art methods by up to 4.4 and 10.8 percentage points. Moreover, with a median detection latency of only 3 ms, the framework meets real-time requirements, providing a robust solution for zero-day attack detection in data-scarce IoV environments. Full article
(This article belongs to the Section Vehicular Sensing)
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19 pages, 2770 KB  
Article
Strain-Specific Variability in Viral Kinetics, Cytokine Response, and Cellular Damage in Air–Liquid Cultures of Human Nasal Organoids After Infection with SARS-CoV-2
by Gina M. Aloisio, Trevor J. McBride, Letisha Aideyan, Emily M. Schultz, Ashley M. Murray, Anubama Rajan, Erin G. Nicholson, David Henke, Laura Ferlic-Stark, Amal Kambal, Hannah L. Johnson, Elina A. Mosa, Fabio Stossi, Sarah E. Blutt, Pedro A. Piedra and Vasanthi Avadhanula
Viruses 2025, 17(10), 1343; https://doi.org/10.3390/v17101343 - 6 Oct 2025
Abstract
SARS-CoV-2 variants have demonstrated distinct epidemiological patterns and clinical presentations throughout the COVID-19 pandemic. Understanding variant-specific differences at the respiratory epithelium is crucial for understanding their pathogenesis. Here, we utilized human nasal organoid air–liquid interface (HNO-ALI) cell cultures to compare the viral replication [...] Read more.
SARS-CoV-2 variants have demonstrated distinct epidemiological patterns and clinical presentations throughout the COVID-19 pandemic. Understanding variant-specific differences at the respiratory epithelium is crucial for understanding their pathogenesis. Here, we utilized human nasal organoid air–liquid interface (HNO-ALI) cell cultures to compare the viral replication kinetics, innate immune response, and epithelial damage of six different strains of SARS-CoV-2 (B.1.2, WA, Alpha, Beta, Delta, and Omicron). All variants replicated efficiently in HNO-ALIs, but with distinct replication kinetic patterns. The Delta variant exhibited delayed replication kinetics, achieving a steady state at 6 days post-infection compared to 3 days for other variants. Cytokine analysis revealed robust pro-inflammatory and chemoattractant responses (IL-6, IL-8, IP-10, CXCL9, and CXCL11) in WA1, Alpha, Beta, and Omicron infections, while Delta significantly dampened the innate immune response, with no significant induction of IL-6, IP-10, CXCL9, or CXCL11. Immunofluorescence and H&E analysis showed that all variants caused significant ciliary damage, though WA1 and Delta demonstrated less destruction at early time points (3 days post-infection). Together, these data show that, in our HNO-ALI model, the Delta variant employs a distinct “stealth” strategy characterized by delayed replication kinetics and epithelial cell innate immune evasion when compared to other variants of SARS-CoV-2, potentially explaining a mechanism that the Delta variant can use for its enhanced transmissibility and virulence observed clinically. Our findings demonstrate that variant-specific differences at the respiratory epithelium could explain some of the distinct clinical presentations and highlight the utility of the HNO-ALI system for the rapid assessment of emerging variants. Full article
(This article belongs to the Special Issue Viral Infection in Airway Epithelial Cells)
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