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Keywords = two-bowl preference test

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15 pages, 865 KiB  
Article
Substitution of Poultry Fat with Black Soldier Fly (Hermetia illucens) Larvae Fat in Dog Diets: Effects on Digestibility, Palatability, Peroxidation of Dry Food, Immunity, Blood Biochemistry, and Faecal Characteristics of Adult Dogs
by Oğuzhan Kahraman, Fatma İnal, Mustafa Selçuk Alataş, Zekeriya Safa İnanç, Samed Damar, Ibrar Ahmed, Mustafa Uludağ and Tamer Çalıkoğlu
Vet. Sci. 2025, 12(4), 311; https://doi.org/10.3390/vetsci12040311 - 29 Mar 2025
Viewed by 630
Abstract
This study evaluated the effects of partially or fully replacing poultry fat with black soldier fly larvae (BSFL) fat on faecal parameters, blood biochemistry, immune responses, nutrient digestibility, food preference, and lipid oxidation in dogs. A total of 18 adult Golden Retriever dogs [...] Read more.
This study evaluated the effects of partially or fully replacing poultry fat with black soldier fly larvae (BSFL) fat on faecal parameters, blood biochemistry, immune responses, nutrient digestibility, food preference, and lipid oxidation in dogs. A total of 18 adult Golden Retriever dogs (6 dogs per diet group) were subjected to a digestibility trial for 30 days. Three experimental diets were formulated: a control diet (6% poultry fat), BSF3 (3% poultry fat +3% BSF larvae fat), and BSF6 (6% BSF larvae fat). Serum biochemical parameters, including blood urea nitrogen, creatinine, AST, ALT, glucose, cholesterol, triglycerides, total protein, and immunoglobulin levels (IgE and IgG), were analysed from blood samples collected from all dogs. Faecal consistency scores were recorded over 5 days, and faecal pH, ammonia nitrogen (ammonia-N), short-chain fatty acids (SCFAs), and branched-chain fatty acid (BCFA) concentrations were determined during the final three days of the digestibility trial. Seven days after the digestibility trial, a modified two-bowl preference test was conducted over four days using 20 dogs to assess the palatability of the control and BSF6 diets. Lipid oxidation in the diets was evaluated by measuring thiobarbituric acid reactive substances (TBARSs, mg MDA/kg) at baseline (day 0) and after 2, 4, 7, and 10 months of storage at ambient temperature (23–25 °C). BSF6 exhibited the lowest dry matter and organic matter digestibility percentages (p < 0.05). Protein digestibility was reduced in both BSF3 and BSF6, while ether extract digestibility was significantly lower in BSF6 compared to the control diet (p < 0.05). The replacement of poultry fat with BSF larvae fat had no significant effect on serum biochemical parameters or IgE and IgG levels (p > 0.05). Faecal acetic acid concentrations were higher in the control group compared to BSF3 and BSF6, whereas faecal valeric acid concentrations were lower in the control group than in the BSF3 and BSF6 groups (p < 0.05). Although faecal pH and consistency scores were not significantly influenced by the dietary fat source, the total SCFA concentrations in faeces decreased with the substitution of BSF larvae fat (p < 0.05). Furthermore, the dogs demonstrated a higher preference rate for the control diet over the BSF6 diet (p < 0.05). In conclusion, the inclusion of BSF larvae fat in dry dog food formulations reduced nutrient digestibility and palatability but had no adverse effects on the health status of the dogs. Full article
(This article belongs to the Section Nutritional and Metabolic Diseases in Veterinary Medicine)
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12 pages, 613 KiB  
Article
Feline Responses to Increasing Inclusion of Natural Olive Extract in Liquid or Dry Palatant Formulations Applied to Kibble Diets
by Catherine Kokemuller, Ryan Guldenpfennig, Clare Hsu and Krysten Fries-Craft
Pets 2025, 2(1), 13; https://doi.org/10.3390/pets2010013 - 9 Mar 2025
Viewed by 620
Abstract
Olive extract (OE) has been used in human foods for its nutraceutical effects, making it a product of interest for pet food. However, OE’s effect on palatability has not been examined. The study objective was to evaluate the palatability of dry cat foods [...] Read more.
Olive extract (OE) has been used in human foods for its nutraceutical effects, making it a product of interest for pet food. However, OE’s effect on palatability has not been examined. The study objective was to evaluate the palatability of dry cat foods with OE applied at differing inclusions within liquid or dry palatants. Twenty-seven volatile compounds were identified by gas chromatography–mass spectrometry for a potentially earthy or fruit-like flavor profile. Liquid palatants were formulated to supply 0 (control), 15, 30, 50, 75, and 150 ppm OE, and dry palatants were formulated to provide 0, 100, 200, 400, and 600 ppm OE when coated onto kibble. Palatability was evaluated using two-day, two-bowl testing of OE-containing versus control rations in adult cats (n = 20) with two-tailed t-tests to determine if OE affected intake ratio (IR). The observed IR of rations with OE were 0.45 to 0.56. The only preference was the 200 ppm treatment (IR = 0.56; p = 0.01) while the other OE rations were not different from the control (p ≥ 0.05). These findings indicate that palatant formulations can supply kibble diets with up to 150 ppm OE for liquid and 600 ppm for dry applications without negatively impacting cat food palatability. Full article
(This article belongs to the Topic Research on Companion Animal Nutrition)
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9 pages, 646 KiB  
Communication
Palatability Testing of Spray-Dried Animal Plasma-Infused Dog Foods and Treats
by Katarzyna Kazimierska, Wioletta Biel and Robert Iwański
Appl. Sci. 2024, 14(17), 7671; https://doi.org/10.3390/app14177671 - 30 Aug 2024
Viewed by 2072
Abstract
The global pet food market is expanding rapidly, and there is a growing interest in sustainable, high-quality ingredients. Spray-dried animal plasma (SDAP), a protein-rich by-product with immune-boosting properties, is gaining attention as a potential additive. This study aimed to evaluate the palatability of [...] Read more.
The global pet food market is expanding rapidly, and there is a growing interest in sustainable, high-quality ingredients. Spray-dried animal plasma (SDAP), a protein-rich by-product with immune-boosting properties, is gaining attention as a potential additive. This study aimed to evaluate the palatability of dog products containing SDAP. Three types of canine products (dry food, wet food, and treats) with varying concentrations of SDAP (0%, 1%, 2%, and 4%) were tested. The study used a two-bowl preference method involving 20 dogs of different breeds, ages, and weights. Results indicated that a 2% SDAP concentration significantly increased the palatability of each dog food compared to control diets without SDAP, while higher concentrations (4%) negatively impacted it. For dry food and treats, the inclusion of 1% SDAP showed marginal effects on palatability. Statistical analyses revealed no significant correlation between the dogs’ sex or product type and their preference for SDAP-containing products (p > 0.05). This preliminary research supports the inclusion of SDAP in dog foods, particularly at optimal levels (2%), to enhance palatability and meet the nutritional needs of dogs while addressing sustainability in pet food production. Full article
(This article belongs to the Special Issue Recent Advances in the Improvement of Food Quality and Safety)
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20 pages, 7331 KiB  
Article
Chemical Composition and Palatability of Nutraceutical Dog Snacks
by Jagoda Kępińska-Pacelik, Wioletta Biel, Małgorzata Mizielińska and Robert Iwański
Appl. Sci. 2023, 13(5), 2806; https://doi.org/10.3390/app13052806 - 22 Feb 2023
Cited by 5 | Viewed by 4526
Abstract
The aim of this study was to evaluate self-produced nutraceutical treats, taking into account the nutritional preferences of dogs, and to analyze the proximate composition, macrominerals and trace elements content. Four variants of snacks were prepared—two extruded and two baked. The snacks consisted [...] Read more.
The aim of this study was to evaluate self-produced nutraceutical treats, taking into account the nutritional preferences of dogs, and to analyze the proximate composition, macrominerals and trace elements content. Four variants of snacks were prepared—two extruded and two baked. The snacks consisted of wholegrain buckwheat flour, wholegrain spelt flour, banded cricket meal, dried hemp inflorescences, dry spirulina biomass, linseed (in the case of baked snacks) and guar gum (in the case of extruded snacks). The proximate composition was determined according to the Association of Official Analytical Chemists methods. Mineral and heavy metal content was analyzed by colorimetry and mass spectrometry. The extruded and baked snacks were analyzed with a scanning electron microscope. The two-bowl test was used as the palatability test. It should be mentioned that when comparing baked snacks to the extruded ones, spaces between starch granules and protein were less visible in the baked snacks but air bubbles were observed suggesting a higher expansion. The mean level of crude fat was twice as high in the baked snacks compared to the extruded snacks. In the case of total carbohydrates, the extruded snacks had a higher content compared to the baked. The analyses showed differences in terms of magnesium content. The average content of trace elements was significantly higher in baked snacks than in the extruded snacks. Dogs preferred the baked variant and the difference between the buckwheat flour content also influenced their preferences—variants richer in buckwheat were chosen less often. This could probably be related to the bitterness in the variant with a higher content of buckwheat flour, which translated into less frequent selection of this variant by dogs. Treats containing insect meal and spirulina can be used in dog nutrition due to their good nutritional value and potential health benefits. Full article
(This article belongs to the Special Issue Advances in Food Flavor Analysis II)
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10 pages, 601 KiB  
Article
Investigating the Palatability of Lamb and Beef Components Used in the Production of Pet Food for Cats
by Pavinee Watson, David Thomas, Adrian Hoggard, Michael Parker and Nicola Schreurs
Animals 2020, 10(4), 558; https://doi.org/10.3390/ani10040558 - 27 Mar 2020
Cited by 2 | Viewed by 4855
Abstract
The pet food industry continues to utilise large amounts of inedible meat components from the human food industry. Although used extensively in pet food formulations and as palatants, little is known about the palatability of individual meat components. The objectives of this study [...] Read more.
The pet food industry continues to utilise large amounts of inedible meat components from the human food industry. Although used extensively in pet food formulations and as palatants, little is known about the palatability of individual meat components. The objectives of this study were to investigate the palatability of raw meat components commonly used in the production of pet food, using acceptance and preference testing. Those examined were lung, heart, kidney, tripe, liver and mechanically deboned meat (MDM) from lamb and beef. Two-bowl acceptance tests were used to develop an overall ranking of components within each species. Two-bowl preference tests between equivalent beef and lamb components were then used to determine whether a preference was exhibited for one species over the other. For the acceptance of components from lamb and beef, liver was the most palatable within both species, with kidney equivalent to liver when testing lamb components. The MDM was identified as the least palatable component from both species. When examining the preference between equivalent components between species, cats showed preferences for lamb over equivalent beef components, except for heart and liver which showed no difference in intake between the two species. Overall, cats were able to clearly rank the palatability of different components from lamb and beef, as well as between equivalent components from the two species. Selecting highly palatable ingredients whilst still meeting pet food manufacturing guidelines may play a role in improving overall diet palatability and acceptance by cats. Full article
(This article belongs to the Section Companion Animals)
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12 pages, 1136 KiB  
Review
Assessing Food Preferences in Dogs and Cats: A Review of the Current Methods
by Christelle Tobie, Franck Péron and Claire Larose
Animals 2015, 5(1), 126-137; https://doi.org/10.3390/ani5010126 - 18 Mar 2015
Cited by 56 | Viewed by 19555
Abstract
Food is a major aspect of pet care; therefore, ensuring that pet foods are not only healthful but attractive to companion animals and their owners is essential. The petfood market remains active and requires ongoing evaluation of the adaptation and efficiency of the [...] Read more.
Food is a major aspect of pet care; therefore, ensuring that pet foods are not only healthful but attractive to companion animals and their owners is essential. The petfood market remains active and requires ongoing evaluation of the adaptation and efficiency of the new products. Palatability—foods’ characteristics enticing animals and leading them to consumption—is therefore a key element to look at. Based on the type of information needed, different pet populations (expert or naïve) can be tested to access their preference and acceptance for different food products. Classical techniques are the one-bowl and two-bowl tests, but complementary (i.e., operant conditioning) and novel (i.e., exploratory behavior) approaches are available to gather more information on the evaluation of petfood palatability. Full article
(This article belongs to the Special Issue Palatability of Pet Food)
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13 pages, 190 KiB  
Review
Pet Food Palatability Evaluation: A Review of Standard Assay Techniques and Interpretation of Results with a Primary Focus on Limitations
by Gregory C. Aldrich and Kadri Koppel
Animals 2015, 5(1), 43-55; https://doi.org/10.3390/ani5010043 - 16 Jan 2015
Cited by 72 | Viewed by 15639
Abstract
The pet food industry continues to grow steadily as a result of new innovative products. Quality control and product development tests for pet foods are typically conducted through palatability testing with dogs and cats. Palatability is the measure of intake of a food [...] Read more.
The pet food industry continues to grow steadily as a result of new innovative products. Quality control and product development tests for pet foods are typically conducted through palatability testing with dogs and cats. Palatability is the measure of intake of a food that indicates acceptance or the measure of preference of one food over another. Pet food palatability is most commonly measured using a single-bowl or a two-bowl assay. While these tests answer some questions about the animals’ perception of the food, there are many limitations as well. This review addresses some of these limitations and indicates opportunities for future research. Full article
(This article belongs to the Special Issue Palatability of Pet Food)
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