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Keywords = ultrafast DNA adsorption

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13 pages, 1960 KB  
Article
SMART: On-Site Rapid Detection of Nucleic Acid from Plants, Animals, and Microorganisms in under 25 Minutes
by Jun-Yuan Ma, Xiao-Fu Wang, Cheng Peng, Xiao-Yun Chen, Xiao-Li Xu, Wei Wei, Lei Yang, Jian Cai and Jun-Feng Xu
Biosensors 2023, 13(1), 82; https://doi.org/10.3390/bios13010082 - 3 Jan 2023
Cited by 1 | Viewed by 2823
Abstract
The rapid on-site nucleic acid detection method is urgently required in many fields. In this study, we report a portable and highly integrated device for DNA detection that combines ultrafast DNA adsorption and rapid DNA amplification. The device, known as silicon film mediated [...] Read more.
The rapid on-site nucleic acid detection method is urgently required in many fields. In this study, we report a portable and highly integrated device for DNA detection that combines ultrafast DNA adsorption and rapid DNA amplification. The device, known as silicon film mediated recombinase polymerase amplification (RPA) for nucleic acid detection (SMART), can detect target DNA in less than 25 min from plants, animals, and microbes. Utilizing SMART, transgenic maize was rapidly detected with high selectivity and sensitivity. The sensitivity threshold of the SMART for transgenic maize genomic DNA was 50 copies. The detection results of genuine samples containing plants, animals, and microbes by SMART were consistent with the conventional polymerase chain reaction (PCR) method, demonstrating the high robustness of SMART. Additionally, SMART does not require expensive equipment and is fast, affordable, and user-friendly, making it suited for the broad-scale on-site detection of nucleic acids. Full article
(This article belongs to the Special Issue Paper-Based Microfluidic Devices and Applications)
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