Search Results (914)

Diabetes mellitus (DM) is a chronic metabolic disorder that can induce systemic and ocular complications. Among the latter, an increase in central corneal thickness (CCT) has been reported, potentially affecting endothelial function and increasing the risk of ocular disease. This study aimed to determine the impact of DM on CCT and to assess its correlation with diabetes duration and glycosylated hemoglobin (HbA1c) levels. A systematic literature search was conducted in Web of Science (1980–2025) following a PICO-based strategy. Observational studies evaluating CCT in diabetic patients were included. Data were analyzed using a random-effects model. Statistical heterogeneity was assessed with χ² test, p values, and I² index. Publication bias was evaluated using Begg’s funnel plot and Egger’s regression test. Twenty-nine studies were included in the meta-analysis. Diabetic patients showed significantly higher CCT values compared to controls, particularly in those with long-standing DM (p < 0.001) and poor glycemic control (HbA1c, p < 0.001). Egger’s regression suggested an association between increasing CCT, disease duration, and HbA1c levels, while funnel plot asymmetry indicated potential publication bias. CCT appears to increase in patients with long-term DM and inadequate glycemic control. These findings highlight the relevance of CCT assessment as a potential indicator of corneal changes in diabetic patients. Full article

Hydrogen peroxide (H₂O₂) occurs in the environment, including in aquatic environments where mosquitoes might lay eggs. However, little is known about the compound’s impact on mosquitoes. We conducted an experiment to determine the effect of H₂O₂ on Ae. aegypti oviposition behavior and egg hatching using H₂O₂ concentrations similar to those in natural aquatic environments. Oviposition behavior was evaluated by dual-choice and multi-choice bioassays. Gravid Ae. aegypti mosquitoes were placed in cages with containers with different H₂O₂ concentrations (5, 25, 50, and 100 μM). After 72 h, the number of eggs laid was compared between oviposition sites with and without H₂O₂. Additionally, egg hatching was assessed under long-period exposure (48 h) and short-period exposure (2, 4, and 6 h and then in deionized water for up to 48 h). Results showed no significant difference in oviposition preference scores in the multi-choice assay (OAI = −0.135 ± 0.06) (p = 0.138), but a significant difference in the dual-choice assay (0.195 ± 0.01) (p = 0.001). Long-period exposure to H₂O₂ did not significantly affect hatch rates (11.34%) (p = 0.363), but short-period exposure significantly impacted hatch rates (17%) (p = 0.0001), with period of exposure alone playing a significant role (p < 0.0044). Eggs exposed to 100 μM H₂O₂ for 2 h (p = 0.0070) and 4 h (p = 0.0036) had significantly higher hatch rates compared to the control. This study demonstrates that low concentrations of H₂O₂ can influence oviposition site characteristics and egg hatch rates. Combined with other environmental factors, H₂O₂ can shape the reproductive success of Ae. aegypti, offering potential strategies for mosquito control. Full article

Background: Inpatient palliative care consultation services operate with an interdisciplinary team, where effective handoffs are crucial for coordinated patient care. We aimed to replace encrypted email handoffs with a more concise and uniform handoff using I-PASS (illness severity, patient summary, action list, situational awareness, contingency planning, and synthesis by receiver) integrated within the electronic medical record (EMR). Aim and Measures: Within six months of launch, our goal was to achieve 90% I-PASS utilization for hospitalized acutely ill patients with cancer receiving palliative care consultation. Intervention: In January 2021, our quality improvement team, consisting of physicians, advanced practice providers, and trainees, began implementing I-PASS using the plan–do–study–act cycle. After providing training sessions for all palliative care clinicians, I-PASS went live on October 1, 2021. I-PASS utilization was tracked via random and monthly audits of EMRs. Through anonymous surveys, both pre- and post-implementation, we gathered clinician feedback and concerns about the handoff system. Survey responses were compared using the Mann–Whitney test. Outcomes: Within six months of implementation, the I-PASS utilization rate reached > 99%. The survey participation rates were 70% (45/64) and 82% (49/60) for the pre-and post-implementation periods, respectively. Respondents provided answers on one to five scale (mean, standard deviation, SD): lower accuracy with email (3.53, SD = 0.98) vs. I-PASS (4.20, SD = 0.83), p < 0.001; handoff lengthier with email (4.17, SD = 1.05) vs. I-PASS (2.1, SD = 1.15), p < 0.001; the time required was longer with email (3.0, SD = 1.22) vs. I-PASS (1.71, SD = 0.73), p < 0.001. Overall, respondents found I-PASS to be significantly better (4.69, SD = 0.58). Conclusion: I-PASS was fully adopted by the team, with nearly 100% utilization and strong clinician endorsement as an effective communication tool. Future efforts should focus on optimizing usability, particularly by educating clinicians on smartphone EMR access and enabling the timely and streamlined editing of I-PASS. Full article

Porous media gas bearings utilize gas as a lubricating medium to achieve non-contact support technology. Compared with traditional liquid-lubricated bearings or rolling bearings, they are more efficient and environmentally friendly. With the uniform gas film pressure of gas bearings, the rotating shaft can achieve mechanical motion with low friction, high rotational speed, and long service life. They have significant potential in improving energy efficiency and reducing carbon emissions, enabling oil-free lubrication. By eliminating the friction losses of traditional oil-lubricated bearings, porous media gas bearings can reduce the energy consumption of industrial rotating machinery by 15–25%, directly reducing fossil energy consumption, which is of great significance for promoting carbon neutrality goals. They have excellent prospects for future applications in the civil and military aviation fields. Based on the three-dimensional flow characteristics of the bearing’s fluid domain, this paper considers the influences of the transient flow field in the variable fluid domain of the gas film and the radial pressure gradient of the gas film, establishes a theoretical model and a three-dimensional simulation model for porous media gas bearings, and studies the static–dynamic pressure coupling mechanism of porous media gas bearings. Furthermore, through the trial production of bearings and performance tests, the static characteristics are verified, and the steady-state characteristics are studied through simulation, providing a basis for the application of gas bearings made from porous media materials in the civil and military aviation fields. Full article

Background/Objectives: The Germ Cell Theory of cancer posits that human primordial germ cells (hPGCs) are the cells of origin for malignancies. While this theory is well established for germ cell cancers, a germ cell origin for somatic cancers has been largely overlooked despite clinical observations of malignant somatic transformation (MST), wherein germ cell cancers give rise to diverse somatic cancer phenotypes, often without additional mutations. Methods: To test the Germ Cell Theory experimentally in somatic cancer, we established a virus-driven MST model linking hPGC-like cells (hPGCLCs) to Merkel cell polyomavirus (MCPyV)-positive Merkel cell carcinoma (MCC), a highly aggressive somatic cancer with a germ cell cancer-like, low-mutation epigenetic profile. The MCPyV genome was transduced into human induced pluripotent stem cells (hiPSCs) or hPGC-like cells by lentiviral transfection, followed by xenotransplantation. Results: Virus-positive MCC (VP-MCC)-like tumors were consistently induced without additional oncogenic mutations. These tumors recapitulated VP-MCC’s high-grade neuroendocrine carcinoma histology and molecular profiles. DNA methylation analysis revealed near-complete global hypomethylation in VP-MCC-like tumors, matching the unique epigenetic state of late-stage hPGCs. Notably, pluripotent intermediates were neither necessary nor sufficient for MST; transformation required acquisition of a late-hPGC-like epigenetic state. Conclusions: This is the first MST model of a somatic cancer arising through an aberrant germline-to-soma transition. Our findings unify VP-MCC and germ cell cancer biology, challenge mutation- and soma-centric paradigms, and provide a tractable platform to investigate developmental and epigenetic mechanisms of oncogenesis. This MST model supports a unifying germ cell origin for both germ cell and non-germ cell somatic malignancies. Full article

Jet fuels are a complex mixture of hydrocarbons and performance additives, including some compounds with established human toxicity. They represent a significant occupational hazard for military personnel; however, little is known about possible long-term health effects, particularly following cessation of exposure. In response to United States (US) Veterans’ concerns, the US Department of Veterans Affairs (VA) and the Department of Defense (DoD) launched a large retrospective cohort study to assess the impact of military occupational jet fuel exposure on Veterans’ health. The Long-Term Impact of Fuel Exposure (LIFE) cohort consists of over 1.3 million Veterans who entered service on or after 1 January 1995, including both individuals with jet fuel-exposed occupations in their service history and a random sample of unexposed Veterans. Data from multiple VA and DoD administrative datasets were linked to evaluate morbidity, disability, and mortality endpoints. Analyses are underway to assess associations between jet fuel exposure and adverse health outcomes in multiple body systems. This study represents the largest effort to date to investigate these effects, with the intention of informing policies affecting Veterans for years to come. Full article

Carbon fiber reinforced polymer (CFRP) composites are prone to developing localized material loss defects during long-term service, which can severely degrade their mechanical properties and structural reliability. To address this issue, this study proposes a multi-sensor synchronous monitoring method combining embedded fiber Bragg grating (FBG) sensors and surface-mounted electrical resistance strain gauges. First, finite element simulations based on the three-dimensional Hashin damage criterion were performed to simulate the damage initiation and propagation processes in CFRP laminates, revealing the complete damage evolution mechanism from initial defect formation to progressive failure. The simulations were also used to determine the optimal sensor placement strategy. Subsequently, tensile test specimens with prefabricated defects were prepared in accordance with ASTM D3039, and multi-sensor monitoring techniques were employed to capture multi-parameter, dynamic data throughout the damage evolution process. The experimental results indicate that embedded FBG sensors and surface-mounted strain gauges can effectively monitor localized material loss defects within composite laminate structures. Strain gauge measurements showed uniform strain distribution at all measuring points in intact specimens (with deviations less than 5%). In contrast, in defective specimens, strain values at measurement points near the notch edge were significantly higher than those in regions farther from the notch, indicating that the prefabricated defect disrupted fiber continuity and induced stress redistribution. The combined use of surface-mounted strain gauges and embedded FBG sensors was demonstrated to accurately and reliably track the damage evolution behavior of defective CFRP laminates. Full article

Mild traumatic brain injury (mTBI) affects over 40 million people every year. One of its features includes the activation of microglia, the resident immune cells of the brain. Microglia assume different morphological states depending on their level of activation, such as surveilling ramified and activated hypertrophic, ameboid, and rod-like microglia. These states can be distinguished by multiple features, including the shape, span, and branching of microglia. Male Sprague–Dawley rats sustained mTBI using the Closed-Head Impact Model of Engineered Rotational Acceleration (CHIMERA) (3 times, 1.5 J per impact) or sham treatment. Four days after the injury, brains were collected and stained for microglia using the ionized calcium-binding adapter molecule-1 (Iba-1) antibody. Cortical injury sites were identified in a subset of CHIMERA animals. Using the MicrogliaMorphology ImageJ plugin and the MicrogliaMorphologyR package, 27 morphological features were quantified from individual microglia, and k-means clustering was used to classify microglia as ramified, rod-like, ameboid, and hypertrophic states. The CHIMERA injury altered microglia morphology features, which contributed to increased hypertrophic (activated) and decreased ramified (inactive) microglia compared to the sham controls. Combined with the clinically relevant mTBI paradigm and semi-automated/unbiased approach, the current findings may contribute to microglia morphology classification. Full article

Emerging research highlights the gut microbiota’s critical role in modulating brain activity via the gut–brain axis. This study explores whether targeted gastrointestinal irradiation induces abscopal effects on the brain proteome, revealing microbiota-mediated neurobiological changes. Male Sinclair minipigs were randomized to receive either sham treatment (n = 6) or 8 Gy lower hemibody (gut-targeted) irradiation (n = 5). Over 14 days, rectal swabs were collected to monitor microbiota dynamics, followed by frontal cortex proteomic analysis. Irradiation altered gut microbiota composition, notably reducing Chlamydiae and Firmicutes phyla, while increasing Coriobacteriaceae and Acinetobacter. Proteomic analysis identified 75 differentially abundant proteins in the frontal cortex, including a significant decrease in pannexin-1 (PANX1), suggesting modulation of the NLRP3 inflammasome pathway. Functional enrichment analysis revealed immune and neurotransmission-related changes linked to microbial shifts. These results demonstrate that gut-targeted radiation can remotely affect brain protein expression, emphasizing the microbiota’s role in neuroimmune regulation and pointing to novel therapeutic opportunities in gut–brain axis disorders. Full article

Fruit bats in the genus Pteropus are the natural reservoirs for zoonotic paramyxoviruses, notably henipaviruses and pararubulaviruses, which are found across Southeast Asia and Oceania. The genetic and antigenic diversity of viruses in both genera, and region specificity, are ill-defined, limiting health security measures aimed at minimizing spillover. For example, Nipah virus has been isolated from bats in the Battambang province of western Cambodia, and surveys suggest bat foraging behaviors occur in close proximity to human settlements. However, there have been no historical cases of Nipah virus in Cambodia. Here, we use a multiplex microsphere immunoassay to identify cryptic human exposure to selected henipaviruses and pararubulaviruses in Cambodia. Convalescent human sera from persons presenting with acute respiratory illness were screened to detect the presence or absence of antibodies reactive with attachment glycoprotein antigens from Nipah virus, Hendra virus, Cedar virus, and Ghana virus, and a hemagglutinin-neuraminidase antigen from Menangle virus. In this sero-survey, we detected antibodies that were specifically reactive with Cedar virus and Menangle virus, including one serum sample that neutralized a recombinant Cedar virus. Additionally, we detected a pattern of cross-reactivity with Hendra virus, Cedar virus, and Ghana virus, suggesting previous infection by an antigenically-related henipavirus. We did not detect high antibody reactivity with the NiV glycoprotein. Future studies should expand serological surveillance for these transboundary pathogens, including genetic surveillance to aid in henipavirus discovery, and focused biosurveillance where interfaces with livestock and humans occur. Full article

This article examines the historical evolution, contemporary dynamics, and future trajectory of China’s legal and judicial framework for pangolin protection. By reviewing over seventy years of regulatory changes, case law, and policy implementation, it outlines three distinct phases: the early emphasis on pangolins as medicinal and export resources (1949–1989); the phase of conflicted protection and utilization under regulatory expansion (1989–2020); and the post-2020 shift toward judicial activism and ecological civil litigation. We then highlight the long-standing contradiction between legislative protection and continued medicinal use, particularly the centuries-old use of pangolins and their derivatives in traditional Chinese medicine, a practice still acknowledged within certain state policies and regulatory frameworks, showing how these inconsistencies enabled persistent illegal exploitation despite regulatory controls. Through systematic analysis of public court records and case databases, the policy historical records reveal a marked increase in environmental public interest litigation since 2020. These lawsuits, often attached to criminal prosecutions, signal a transition from merely punitive approaches to restorative ones—anchored in ecological valuation of species and their services. Case studies illustrate how courts now impose not only wildlife resource loss fees, but also punitive damages and compensation for ecological service function loss. The article will elaborate in detail on the distinctions and interrelations among these types of compensation. The landmark Case No.17 also demonstrates this paradigm shift, wherein courts recognized pangolins’ role in balancing forest ecosystems. However, significant challenges persist. Valuation methodologies lack uniform standards; while the ecological value of pangolins has been recognized, their inherent value as individuals has not been emphasized within the legal system; judicial discretion varies across jurisdictions; and public interest organizations remain underutilized in litigation. Moreover, while the crackdown on organized crime succeeded in curbing mass trafficking, smaller-scale violations tied to cultural consumption for medicine use persist. The article concludes that judicial innovations, such as ecological judicial restoration bases and integration into China’s draft Ecological Environment Code, offer promising pathways forward. To enhance efficacy, it calls for standardization in ecological valuation, strengthened civil society participation, and nuanced differentiation in penal strategies between minor and serious offenses. This study ultimately positions judicial reform as the cornerstone of China’s evolving pangolin conservation strategy. Full article

To address incomplete die filling, high cracking tendency, and severe die wear in the conventional forging of AISI-410 martensitic stainless steel U-shaped forgings, an optimized billet volume pre-allocation strategy was proposed. Two improved forging schemes for the U-shaped forgings were designed: the Arc Concave Flattening Scheme (adding arc-shaped concave features to the flattening die for corner volume compensation) and Preformed Volume Allocation Scheme (incorporating a preforming step for strategic volume pre-allocation at ends and corners). Finite Element Analysis employing the Oyane damage model and Archard wear model was employed to simulate and optimize the forging process. The optimal scheme was applied to production trials. The results demonstrated that the Preformed Volume Allocation Scheme significantly improved the geometric compatibility between the billets and the final forging die cavity. As a result, the billet’s temperature, strain, and equivalent stress uniformity increased, reducing cracking tendency. Moreover, the rise in the mitigated temperature and stress concentration resulted in reduced final forging die wear. Production trials confirmed a qualified rate of ~96% (34% higher than the Original Scheme). The final forging die service life reached 300 pieces per refurbishment cycle, showing a 50% improvement. This work provides theoretical and practical guidance for optimizing the forging processes of complex martensitic stainless steel components. Full article

As a novel type of ocean monitoring tool, unmanned sailboats exhibit significant application potential. In this study, a novel wing sail structure for offshore unmanned sailboats is proposed and its performance compared with that of the conventional NACA 0021 wing sail. The Reynolds-averaged Navier–Stokes (RANS) equations are employed for numerical analysis, and the aerodynamic performance is evaluated using ANSYS Fluent. The results indicate that the lift coefficient and lift-to-drag ratio of the HF-14-CE-01 wing sail are significantly superior to those of the NACA 0021 wing sail. Compared to the NACA 0021 wing sail, the HF-14-CE-01 wing sail has undergone structural optimization. The HF-14-CE-01 wing sail demonstrates improved wind direction efficiency, uniform force distribution, ease of adjustment, and extends the service life of the sail. Subsequent research examined the influence of aspect ratio on both the aerodynamic performance of the wing sail and the thrust generated by the unmanned sailboat, identifying an optimal aspect ratio of 4 for the HF-14-CE-01 wing sail. Analysis of the velocity and static pressure contour maps for the HF-14-CE-01 wing sail identified a critical angle of attack of 28°, providing a clear visual representation of its aerodynamic performance. Furthermore, compared with other rigid sail designs, the HF-14-CE-01 wing sail achieved a 30.9% increase in peak lift coefficient, indicating superior propulsion capability. Full article

The objective of this study was to characterize the sexual behavior and reproductive performance of Colombian Creole bulls from the Romosinuano (ROM) and Costeño con Cuernos (CCC) breeds, to support their strategic use in tropical production systems and sire selection programs. A standardized sexual behavior test, including nine behavioral indicators, was conducted over a 15 min observation period to assess libido and service capacity. Significant differences (p < 0.05) were found between the breeds in terms of the frequency of urination and mounting behaviors. ROM bulls exhibited a more uniform and rapid behavioral response, while CCC bulls showed greater individual variability and a broader behavioral repertoire, with courtship behaviors—such as smelling, the Flehmen reflex, and butting—strongly associated with ejaculation events. Libido scores were high in both breeds, with 80.35% of bulls rated as very good to excellent. CCC bulls also achieved mounts more frequently within the first five minutes of exposure. Additionally, bull age was inversely associated with mounting time (p < 0.05), suggesting that maturity and sexual experience influence behavioral efficiency. These findings represent the first quantitative assessment of sexual behavior in CCC bulls and provide comparative insights with ROM bulls, highlighting the functional reproductive potential of Colombian Creole bulls under low-input tropical conditions. Full article

Polytrauma is a critical global health concern characterized by immune dysregulation and a high risk of multiple organ dysfunction syndrome (MODS). Early molecular mechanisms linking trauma severity to organ injury are poorly understood. We used two rat blast-polytrauma models: a tourniquet-induced ischemia/reperfusion injury (tIRI) model and a non-ischemia/reperfusion injury (non-IRI) model. Naïve animals served as controls. RT-qPCR of 120 inflammatory genes in the lung, kidney, and liver, combined with STRING protein–protein interaction analysis, revealed distinct yet overlapping inflammatory gene signatures across all the organs. A core set of genes (Il6, Lbp, Nos2, and Lcn2) was consistently upregulated, indicating shared inflammatory pathways. Transcriptomic responses were most pronounced in the tIRI group, with greater magnitude and altered temporal dynamics, uniquely amplifying pro-inflammatory cytokines, immune cell activators, chemokines, and tissue damage markers. Lipocalin-2 (Lcn2/NGAL) emerged as a shared hub gene across all the organs within 24 h post-injury. Its expression significantly correlated with MODS activity and adverse outcomes, independent of the injury model. At 168 h, Lcn2 expression correlated with increased liver damage and NGAL levels correlated with tissue trauma severity. These findings elucidate distinct pro-inflammatory mediators and networks underlying secondary organ dysfunction, highlighting NGAL as a potential universal biomarker of trauma-induced inflammation and MODS activity, suggesting it as a therapeutic target. Full article