Search Results (695)

Background: The nation’s defense relies on a cadre of young individuals with strong physical and mental health. The study aimed to identify health behavior-related correlates of physical and mental health in future conscripts. Methods: This cross-sectional study comprised 676 male school students with an average age of 18.48 (1.15) years. The measures of weekly physical activity, nutrition (Kidmed questionnaire), psychological well-being (WHO-5 Well-Being Index), psychological distress (Kessler scale), cardiorespiratory fitness (20 m Shuttle Run test), weight, and height for calculation of body mass index were taken. Results: Among potential conscripts (17–19-year-old males), 22% have poor cardiorespiratory fitness. More than a quarter of young males have poor psychological well-being. Better cardiorespiratory fitness is related to more recommendations-adherent nutrition (Std β = 0.133 [0.03–0.14], p < 0.05). Engagement in sports is related to better cardiorespiratory fitness (Std β = 0.202 [0.10–0.30], p < 0.05). Higher psychological distress is associated with more time spent passively (Std β = 0.145 [0.12–0.34], p < 0.01); better psychological well-being is associated with more adherent nutrition (Std β = 0.172 [0.14–0.34], p < 0.0001), more hours accumulated in moderate-to-vigorous physical activity (Std β = 0.150 [0.30–1.10], p < 0.01), and less time spent passively (Std β = −0.131 [−0.34–−0.11]). Conclusions: Potential conscripts’ physical fitness and mental health are related to better health behaviors. Behavior change practices and policies applied at school could benefit potential conscripts and youth in general in terms of their physical and mental health. Full article

Cannabis sativa is a short-day (SD) plant, producing inflorescences when the daily scotoperiod (period of darkness) exceeds approximately 10 h of length. As such, the vegetative photoperiod is typically maintained at 16 to 18 h, which limits the scotoperiod to no more than 6 to 8 h and keeps plants in the vegetative stage. The electricity cost associated with supplemental lighting is a major concern for controlled environment cannabis cultivation. Therefore, the strategy of utilizing a 12 h photoperiod while interrupting the scotoperiod with a 1 h “night break” (NB) is appealing, as it reduces the overall electricity required for supplemental lighting by nearly one third, while maintaining vegetative growth. Our study tested the feasibility of this method under controlled indoor conditions. We studied the effect of the NB method (as compared to conventional light/dark periods) on cannabinoid and extractable biomass yields, as well as phenotype. Reducing vegetative DLI via the NB method (29.4 → 21.2 mol m⁻² d⁻¹) reduced extractable floral biomass by ~22% (control 1295 g vs. NB 1015 g per tent), while cannabinoid concentrations were similar between treatments. We also found that NB plants were less vigorous and shorter, with shorter internodes and fewer branches. This evidence suggests that although the NB method may reduce electricity costs during the vegetative stage of controlled environment cannabis growth, the method is not economically feasible due to the loss of yield and plant vigor. Full article

Marine actinomycetes constitute a vigorous source of bioactive compounds with potential anti-tumor activity. This study investigates the antitumor activity and classification of actinomycetes isolated from 32 marine soil samples collected across four seasons from Tyr City Beach, Lebanon. A total of 80 morphologically diverse isolates were recovered and characterized, with dominant genera including Streptomyces, Kocuria, and Micrococcus. Among these, three promising strains—Kocuria rosea, Micrococcus luteus, and Streptomyces longisporoflavus—were selected for further analysis. Crude extracts were tested against human colorectal adenocarcinoma (Caco-2) and human hepatocellular carcinoma (HepG-2) cancer cell lines using MTT and Western blot assays. At the highest concentration (8 µg/µL), the extracts reduced cell viability to 24–37% in Caco-2 and 12–25% in HepG-2. The IC₅₀ values ranged from 1.72 to 3.53 µg/µL, depending on the extract and cell line. Western blot analysis showed dose-dependent increases in the Bax/Bcl-2 ratio, with fold changes reaching 4.35 (Kocuria), 11.39 (Micrococcus), and 14.25 (Streptomyces) in HepG-2 cells. The p53 protein expression also increased significantly, with fold changes up to 7.79 in Caco-2 and 3.0 in HepG-2 cells. These results indicate that marine actinomycetes from the Lebanese coastline hold strong potential as a source of antitumor agents targeting apoptosis pathways. Full article

Background: Rising sedentary behavior and declining physical activity (PA) among youth are major public health concerns. Evidence on how different PA types affect fitness in Chinese children is limited. PA indicators were classified according to the Global Matrix 4.0, an international framework for benchmarking PA in children and adolescents. Objective: To identify which PA types and frequencies, based on the Global Matrix 4.0, are most associated with physical fitness in Chinese youth. Methods: Data from 102,942 children aged 9–18 were analyzed. Descriptive statistics summarized demographics, PA levels, and physical fitness rates. Chi-square tests assessed group differences. Binary logistic regression examined associations between PA and physical fitness, adjusting for gender, age, school level, parental education, and urban–rural residence, with cluster-robust standard errors at the school level. Results: Higher frequencies of moderate-to-vigorous physical activity (MVPA), muscle-strengthening exercise (MSE), and organized sports and physical activity (OSPA) were significantly associated with better fitness (p < 0.001). OSPA (OR = 1.23) and MSE (OR = 1.21) showed the strongest associations. Sedentary behavior was negatively associated with fitness (p < 0.001). Conclusions: Regular engagement in MVPA, MSE, and OSPA, alongside reduced sedentary time, promotes physical fitness among Chinese children and adolescents. Policies should support diverse and consistent PA participation. Full article

Through highly detailed data acquisition, a precision agriculture approach leads to the optimization of inputs, improving, for instance, water and nutrient use efficiency. High-resolution vigor mapping in perennial orchards provides the spatial detail required to achieve such targeted management. This exploratory case study characterizes the spatial variability of vegetative vigor in a young SHD almond orchard in southern Sardinia by integrating high-resolution unmanned aerial vehicle (UAV) imagery and Normalized Difference Vegetation Index (NDVI) mapping with two consecutive seasons of ground measurements; the NDVI raster was subsequently used to delineate three distinct vigor zones. The NDVI was selected as a reference index because of its well-assessed performance in field-variability studies. Field measurements, during the kernel-filling period, included physiological assessments (stem water potential (Ψ_stem), SPAD, photosynthetic rates), morphological evaluations, soil properties, yield, and quality analyses. High vigor zones exhibited better physiological conditions (Ψ_stem = −1.60 MPa in 2023, SPAD = 38.77 in 2022), and greater photosynthetic rates (15.31 μmol CO₂ m⁻² s⁻¹ in 2023), alongside more favorable soil conditions. Medium vigor zones showed intermediate characteristics, and balanced soil textures, producing a higher number of smaller almonds. Low vigor zones exhibited the poorest performance, including the most negative water status (Ψ_stem of −1.94 MPa in 2023), lower SPAD values (30.67 in 2023), and coarse-textured soils, leading to reduced yields. By combining UAV-based NDVI mapping with ground measurements, these results highlight the value of precision agriculture in intra-field variability identification, providing a basis for future studies that will test site-specific management strategies in SHD orchards. Full article

Background and objectives: The objective of this study was to test the effects of objectively measured screen time using wearable cameras, along with dietary intake and physical activity level (and their interaction), on obesity among Chinese school-aged children. Methods: This study was conducted among 52 fourth-grade children (age: 9.76 ± 0.44; 50% boy) in Beijing, including children with obesity and age- and sex-matched normal-weight controls. Screen time (min/day) was coded from wearable camera images collected over one week using image recognition, physical activity measured using accelerometers, and dietary intake via camera-assisted 3-day 24 h dietary recalls. Logistic regression and generalized additive models assessed associations with obesity indicators, including general (obesity; percentage of body fat, BF%) and central (waist circumference; weight-to-height ratio). The combined effects of screen time with dietary and physical activity factors were also analyzed. Results: Children with obesity had longer daily screen time (94.91 ± 35.44 vs. 83.15 ± 36.86 min). Longer screen time was associated with higher energy and carbohydrate intake, more average duration per meal, a higher proportion of meals with screen, increased sedentary time, and a lower proportion of time spent in moderate-to-vigorous physical activity (MVPA). After adjusting for dietary intake and demographic covariates, longer screen time (≥1.3 h/day) was linked to higher odds of obesity (OR = 4.25, 95% CI = 1.09, 16.53) and BF% (OR = 6.14, 95% CI = 1.29, 29.10). Less protein intake (OR = 9.57, 95% CI = 1.31, 70.14), more proportion of meals with screen (OR = 6.40, 95% CI: 1.22, 33.61), less proportion of meals with social interaction (OR = 5.90, 95% CI: 1.01, 34.59), and less MVPA (OR = 5.21, 95% CI = 1.11, 24.43) with more screen time increased obesity risk. Conclusions: Objectively measured screen time was positively associated with the risk of childhood obesity. Longer screen time combined with lower protein intake, a higher proportion of meals consumed while watching screens, less meals with social interaction, and lower MVPA may collectively increase obesity risk. These findings call for efforts to reduce screen exposure, promote MVPA, and increase dietary protein intake. Additionally, encouraging children to avoid screen use during meals and promoting parent or peer companionship during eating may help reduce the risk of childhood obesity. Full article

This study aimed to evaluate the effects of different storage conditions on seed viability, vigor, and agronomic performance in six forage pea (Pisum sativum L. var. arvense) cultivars: Uysal, Özkaynak, Kurtbey, Ürünlü, Taşkent, and Nany. The research was conducted under Manisa (Türkiye) field conditions during the 2021–2022 growing season, with supporting laboratory tests performed in Ankara. Seeds were evaluated before and after a three-month storage period under two conditions: room temperature and cold storage (5 °C, 60% RH). Laboratory analyses included germination percentage, germination speed, electrical conductivity (EC), and field emergence tests. Results revealed that cold storage significantly preserved seed vigor and viability, as indicated by lower EC values, higher germination and field emergence rates. Among cultivars, ‘Taşkent’ and ‘Nany’ demonstrated superior physiological seed quality, while ‘Uysal’ was more sensitive to adverse storage conditions. In field trials, cold-stored seeds produced taller plants, earlier flowering, and higher green and dry forage yields compared to room-stored seeds. The cultivar ‘Ürünlü’ stood out with the highest average forage yield. These findings underline the importance of genotype-specific responses and proper seed storage practices to maintain seed quality and optimize forage productivity in cool-season legumes. Full article

Background/Objectives: This study examined the efficacy of a physical activity (PA) intervention augmented by a non-immersive Virtual Reality (VR) gaming system (i.e., Virtual Reality-infused Movement and Activity Program; V-MAP) on physical activity (i.e., sedentary behavior, moderate-to-vigorous PA [MVPA], vigorous PA [VPA]) and cognitive skills (i.e., response error, movement latency and reaction time) in Head Start preschoolers. Methods: Using a repeated-measure with 1-month follow-up design, a sample of 13 Head Start preschoolers (Mage = 67.08 ± 4.32 months; 36.2% boys) engaged in a 6-week V-MAP intervention (30-min session; 8 sessions) that focused on non-immersive VR based movement integration. The Cambridge Neuropsychological Test Automated Battery (CANTAB) was used to measure cognition; school-based PA and sedentary behavior were assessed by ActiGraph accelerometer. Pedometers were used to monitor real time engagement and implementation over eight intervention sessions. Results: On average, children obtained 1105 steps during the 30-min intervention (36.85 steps/min). There was a significant increase in VPA after the V-MAP intervention, whereas no significant changes in MVPA or sedentary behavior were observed (ps > 0.05). Although we did not observe significant improvement in studied cognitive function variables (ps > 0.05) after the V-MAP intervention, some delayed effects were observed in the follow-up test (Cohen’s d ranges from −0.41 to −0.73). Conclusions: This efficacy trial provides preliminary support that implementing V-MAP in recess may help Head Start preschoolers achieve or accumulate the recommended daily 60-min MVPA guideline during preschool years. The findings also provide insights that VR-based PA for as little as 30 min per day may benefit cognitive capability. Full article

Rice lipoxygenase 3 (OsLOX3) is a lipid hydroperoxidase found in rice embryos. Previous studies have reported that OsLOX3 is associated with seed quality and stress resistance, however, its relationship with grain shape and weight remains unknown. In this study, the first exon of OsLOX3 gene was edited in the indica rice variety GDR998 using CRISPR/Cas9 technology. Two homozygous mutants, Oslox3-1 (single-base deletion) and Oslox3-2 (single-base insertion) were identified among eight positive mutant plants from the T2 generation. The agronomic evaluation of genotypic OsLOX3 mutants showed significant increase in grain length, grain length-to-width ratio, 1000-grain weight, plant height, panicle length, and yield per plant compared with the wild type GDR998. The number of effective panicles and total grains per panicle did not significantly change. Further germination tests of seeds after three years of natural aging revealed that, compared with the control GDR998, the germination percentages of the mutants Oslox3-1 and Oslox3-2 increased significantly by 41.1% and 45.6%, respectively. These findings indicate that the knockout of OsLOX3 simultaneously improve grain weight and seed vigor, providing valuable germplasm resources for rice breeding targeting high-yield, improved seed longevity and rice quality. Full article

Objectives: To analyze, across the periods before, during, and after the implementation of Social Isolation and Distancing Measures (IMDIS): (a) changes in the prevalence of non-communicable chronic diseases (NCDs), stratified by age group in the Brazilian population; and (b) the association between physical inactivity (PI), insufficient moderate-to-vigorous physical activity (MVPA), and sedentary behavior (SB) with the occurrence of these conditions. This cross-sectional study used data from VIGITEL (Brazil’s Surveillance System of Risk and Protective Factors for Chronic Diseases by Telephone Survey), an annual population-based telephone survey conducted across the country. Data were collected in 2019, 2021, and 2023, with a total sample size of 101,226 participants. Arterial hypertension (AH) and diabetes mellitus (DM) were self-reported, and obesity (OB) was diagnosed using body mass index. PI, insufficient MVPA, and SB were identified via VIGITEL indicators. Chi-square tests assessed differences in prevalence overall and by age group. Logistic regression models estimated odds ratios (ORs) for associations between demographic variables, behavioral factors, and the studied periods. The prevalence of AH and DM was highest among individuals over 60 years, reaching 61% after IMDIS, a period when OB also peaked across all age groups. Individuals aged 30–59 and those over 60 had higher odds of AH, DM, and OB across all periods. Female participants had higher ORs for AH and DM both before and after IMDIS. PI and insufficient MVPA were associated with increased odds of AH, DM, and OB in all periods, while SB significantly elevated the OR for OB at all time points. After IMDIS, there was an increase in the prevalence of AH, DM, and OB among older adults and younger individuals. PI, insufficient MVPA, SB, and advanced age were all associated with a greater likelihood of NCDs at every stage of the study. The high post-IMDIS rates of AH, DM, and OB highlight the need for urgent public health strategies. Low-cost programs, such as live videos and online group sessions, should be included in national physical activity guidelines. These initiatives are affordable, aligned with WHO goals, and reduce PI in IMDIS scenarios. Incorporating them into Academia da Saúde and Agita Brasil strengthens NCD prevention and increases the resilience of the health system for future health crises. Full article

Due to the widespread distribution of F. oxysporum, the search for mechanisms of tolerance to this disease in Solanum lycopersicum L. is an ongoing endeavor. This research aimed to identify F. oxysporum-tolerant genotypes at the germination and seedling stages in order to use them as sources of resistance. Ninety-six tomato lines were inoculated with the F. oxysporum strain with NCBI accession key PQ187438. The germination test was carried out in a germination chamber at a constant temperature of 28 ± 2 °C with 70 ± 5% relative humidity in darkness for the first 3 days and then 7 days with light. Clustering and discriminant analysis identified 14 genotypes with tolerance, showing great seed vigor and lower disease severity. Seedling evaluation was conducted in a floating raft system for 10 days after inoculation. Nine genotypes showed greater tolerance to the pathogen by developing a larger leaf area and accumulating more dry matter (p ≤ 0.05). No genotypes with tolerance were identified at both phenological stages (germination and seedling), indicating that tolerance mechanisms are independent at both phenological stages, so genotype selection should be carried out independently. Full article

Objectives: This study aimed to assess changes in anthropometric measures, cardiometabolic markers, and physical fitness following a structured basketball training program in healthy prepubertal boys. Methods: The intervention consisted of a 6-week pre-season phase followed by a 32-week basketball training season conducted during the academic year. Training sessions were held three times per week at moderate to vigorous intensity, along with a weekly match. The participants were assessed at baseline, 6, 9, and 12 months. A reference group was evaluated at baseline for comparison. The study was registered at ClinicalTrials.gov (identifier: NCT07007624). Results: Seventeen boys completed the program. Anthropometric assessments revealed increases in fat-free mass in the trunk and lower limbs, along with maintenance of an adequate BMI. After nine months, participants in the intervention showed significant improvements in fitness tests, including a 45% increase in Course Navette performance (p < 0.001), a 21% increase in horizontal jump performance (p = 0.001), and a 13% increase in abdominal test performance (p < 0.001). Conclusion: These findings suggest that a structured, school-based basketball program may enhance physical fitness and support healthy body composition maintenance in healthy-weight prepubertal boys. Full article

Maturity estimation before seed collection is necessary in reducing the costs of seed collection; it allows vigorous seeds to be collected, ensuring that maximum germination will be reached and producing quality planting stock. In addition to this, appropriate temperature, seed size, pH, light, and stress conditions also influence germination. Cones of Cedrus deodara were collected at different intervals to estimate the maturity of the cones. A seed germination test was conducted in the laboratory under constant temperature, seed size, pH, light conditions, and water and salinity stress conditions. Significant (p < 0.05) variations in cones, such as seed morphological characteristics, germination, and related parameters, of C. deodara at different maturity periods were observed. The morphological traits of cones, such as seed weight, seed length, seed width, and seed germination, increased with increasing maturity, while the cone weight, moisture contents, specific gravity, and seed moisture decreased with increasing maturity. A constant temperature of 15 °C to 20 °C (98.0% to 92.0%) and the use of large-sized seeds (99.0%) led to maximum germination. Lower concentrations of Polyethylene glycol (98.0%) and NaCl (78.0%) contributed to maximum seed germination. The germination of C. deodara is temperature-dependent and seed size, light, and high water and salinity stress significantly influence seed germination. Full article

Seeding depth is a critical factor influencing the uniformity and vigor of wheat seedlings. To address inconsistent seeding depth in wide-row uniform seeding agricultural practices, we performed parameter analysis and optimization experiments on the seeding depth device of a wheat wide-row uniform seeding machine. The structure and working principle of the device were described, soil movement during operation was analyzed, and the models of rotary tiller blades and soil retention plates were investigated, identifying three key factors affecting seeding quality. Using the discrete element method, a model of the seeding depth device was established, and experiments were conducted, yielding the following conclusions: 1. Single-factor experiments were conducted under different seeding rate conditions, and it was found that the effects of various factors on the two indicators, namely the seeding depth qualification rate and the coefficient of variation for seeding uniformity, were regular. 2. A quadratic orthogonal rotated combination experiment with three factors determined the optimal structural parameters: tillage device penetration depth of 120 mm, rotational speed of 310 rpm, and soil retention plate inclination angle of 27°. Under these parameters, the seed depth qualification rate exceeded 90%, and the coefficient of variation for seed distribution uniformity was below 25%. 3. Field validation tests under optimal parameters confirmed a seed depth qualification rate ≥90% and variation for seed distribution uniformity was below ≤20.69%. 4. The error between simulation and field tests was ≤5%, validating the reliability of the discrete element method-based optimization for the seeding depth device. Full article

Vigor-reducing rootstocks are now commonly used in sweet cherry cultivation. However, their application in Hungary presents challenges due to the drier summer climate and limited availability of irrigation water. The aim of this study was to determine the water transport characteristics and potential drought tolerance of three vigor-reducing rootstocks that may be suitable for cherry production in Hungary. The stomatal conductance (gs), midday stem water potential (MSWP), and sap flow velocity were measured in four-year-old Carmen and Regina cherry trees grafted onto MaxMa 14, WeiGi 2, and GiSelA 6 rootstocks. Measurements were taken after harvest during a period of severe drought. Among the rootstocks studied, MaxMa 14 trees exhibited the lowest MSWP values, even after irrigation and during periods with a relatively adequate water supply. No significant or consistent differences in the gs values were observed between the rootstocks. However, the variation in the gs and MSWP values before and after irrigation was the greatest in MaxMa 14 trees and the smallest in GiSelA 6 trees. Furthermore, the sap flow velocity in MaxMa 14 trees showed no significant difference between the pre- and post-irrigation measurements, indicating stable water transport. In contrast, trees on GiSelA 6 and WeiGi 2 rootstocks exhibited significant differences between dry and irrigated conditions. Although MaxMa 14 showed lower MSWP values, its gs responded more dynamically to changes in the water availability, and it maintained consistent water transport parameters across both dry and wet conditions. Based on the evaluated parameters, GiSelA 6 and WeiGi 2 showed similar behavior. However, in regard to some traits—such as the dynamic change in stomatal conductance—WeiGi 2 appeared to be more similar to MaxMa 14. Our results suggest that MaxMa 14 may be the most adaptable to drought among the tested rootstocks. Full article