Search Results (1,518)

The tung tree, a crucial woody oil plant, serves as a premium raw material for eco-friendly coating production, yet its short lifespan (typically under 20 years) and lack of asexual reproduction have led to resource losses. This study surveyed wild tung trees in the Hunan–Guizhou region, focusing on older and high-fruit-yielding specimens. After two years of investigation, selected individuals were conserved in the Wugang Tung Tree Germplasm Resource Bank to provide high-quality materials for breeding superior varieties. Comparative analysis of fruit yield and commercial traits from 60 wild trees identified 12 superior individuals for secondary selection, with notable trait variations observed. Using the entropy weight-TOPSIS method, superior individual FT01 exhibited the highest relative closeness (C = 0.6836), indicating optimal overall traits, while FT01, XY12, JX01, WG25, and WG31 (all with C > 0.50) demonstrated good overall performance. Genetic diversity analysis of these 12 individuals, employing 14 SSR primers, revealed 33 alleles (average 2.2142 per primer), Shannon’s information index values ranging from 0.1973 to 0.9723 (average 0.5325), and polymorphism information content between 0.1486 and 0.5833 (average 0.3981), indicating high genetic diversity. UPGMA clustering divided the superior trees into five groups, with FT01, WG25, JX01, and XY12 in separate groups, all exhibiting high yield and large fruit size, consistent with TOPSIS results. Consequently, FT01, XY12, JX01, WG25, and WG31, with the highest comprehensive evaluation scores and richest genetic diversity, are prioritized as candidate materials for new variety selection and breeding. Full article

Crataegus harbisonii Beadle (Harbison’s or Harbison hawthorn) is a Tennessee (USA) endemic tree of the Rosaceae family, currently considered “critically imperiled” at the state, national, and global levels. It is known from only two extant wild locations, one in Davidson County, Tennessee consisting of a single living individual and a population of less than 100 individuals in Obion County, Tennessee. Key ex situ conservation efforts undertaken over the last three years with this critically imperiled species are reported here. The Obion County population was intensively surveyed and all C. harbisonii individuals documented. Over three seasons, seeds were collected and propagated, and clones were generated via chip-budding and grafting. Conservation seed orchards were planned and established to provide a stable, long-term source of genetically robust seed for reforestation and research. To date, 19 sources from the Obion County location as well as the single Davidson County genotype have been successfully preserved through clonal propagation, and open-pollinated seedlings produced from 12 unique mother trees. Additional material is being added annually. We report lessons learned as well as key future research directions, now enabled through the establishment of germplasm resources. Full article

Environmental stress drives plant communities toward conservative ecological strategies through increased wood density (WD) within the Wood Economics Spectrum (WES). However, hyper-arid regions like the Coastal Atacama Desert (CAD) challenge this pattern, where woody plants exhibit acquisitive traits and decreased WD with increasing aridity. The underlying anatomical mechanisms remain poorly understood. This study examined how extreme aridity in the CAD shapes wood economics strategies, testing whether anatomical changes prioritize hydraulic safety over efficiency within the WES. Six shrub communities along an aridity gradient were sampled, measuring composition and abundance of 29 species across 20 plots per site. Community-weighted means of eight wood traits—including WD, vessel density/diameter, parenchyma fraction, fiber fraction, and lumen fractions—were analyzed. PCA, triangular and linear models assessed trait variation along the aridity gradient. Unexpectedly, WD increased at both gradient extremes but through different tissue compositions, with no clear shift from acquisitive to conservative strategies. Instead, vessel traits (density and diameter) were key, reflecting an independent hydraulic safety–efficiency trade-off. PC2 strongly correlated with aridity, showing reduced vessel size and increased density under greater aridity. Findings reveal that hyper-aridity disrupts traditional wood economics, with hydraulic adaptation—not WD-mediated resource use—driving community assembly. This highlights the dominance of safety–efficiency trade-offs in structuring shrub communities in extreme deserts, emphasizing hydraulic traits over conventional resource strategies. Full article

Background: Transcription factors (TFs) critically regulate gene expression, orchestrating plant growth, development, and stress responses. The conserved IDD (INDETERMINATE DOMAIN) TF family modulates key developmental processes, including root, stem, and seed morphogenesis. Dendrocalamus latiflorus Munro, an economically vital sympodial bamboo in southern China, suffers significant yield losses due to prevalent bamboo shoot abortion, impacting both edible shoot production and timber output. Despite the documented roles of IDD TFs in shoot apical meristem expression and lateral organ regulation, their genome-wide characterization in D. latiflorus remains unstudied. Methods: Using IDD members from Arabidopsis thaliana, Oryza sativa, and Phyllostachys edulis as references, we identified 45 DlIDD genes in D. latiflorus. Comprehensive bioinformatics analyses included gene characterization, protein physicochemical assessment, phylogenetic reconstruction, and examination of gene structures/conserved domains. Differential expression of DlIDD genes was profiled between dormant and sprouting bamboo shoots to infer putative functions. Results: The 45 DlIDD genes were phylogenetically classified into three subfamilies and unevenly distributed across 34 chromosomes. Whole-genome duplication (WGD) events drove the expansion of this gene family. Promoter analyses revealed enriched cis-regulatory elements associated with hormone response and developmental regulation. Functional analyses suggested potential roles for DlIDD genes in bamboo shoot development. Conclusions: This study provides a foundation for future research to elucidate the functions of IDD TFs and their regulatory mechanisms in bamboo shoot morphogenesis and lateral bud development within woody monocots. Full article

Pharomachrus mocinno breeds in the cloud forests of the El Triunfo Biosphere Reserve, and migrates annually for six months to elevations of 900–1600 m. On the Gulf slope, temperate forests were identified as habitats for migration, but the forests on the Pacific slope have not been similarly described. In this study we described the emergent properties and phenological behavior of the plant communities of five sites identified as migration habitats, in order to test if the number of fruit-bearing species is related to the migration period. At each site, 10,000 m² was sampled, for which PBH (perimeter at breast height) and the height of shrubs and trees were annotated, including the number of palms and ferns included. We identified 25 orders, 41 families, 71 genera, and 94 species; 86.6% of these species produce fleshy fruits or fruits with modified structures that are eaten by Quetzals. During the migration period, 25–43% of these species have fruits. Eight woody species included 49% of the total individuals, which produce Quetzals’ feeding resources. The sites differed in vertical structure, composition and diversity levels. The rarefaction curve indicated that the upper site (1600 m) required more sampling. We identified three plant communities that were distributed either in montane rain forest or in the temperate forest. Since nearly 84% of the plant species are listed in the IUCN (International Union for Conservation of Nature), these forests have an intrinsic importance. The number of fruit-bearing species did not differ between migration and breeding seasons (X² (1, N = 76) 0.57; p = 0.32. Lauraceae did not stand out for the number of fruit-bearing species in any of the migration sites. Full article

The AP2/ERF transcription factor family plays pivotal roles in plant growth, stress responses, and defense mechanisms, yet its diversity in camphor trees remains underexplored. This study identified 154 AP2/ERF genes in the Camphora officinarum genome, with over 80% belonging to the ERF subfamily, a distribution consistent with other angiosperms. Synteny analysis revealed that tandem and segmental duplications were key drivers of family expansion, suggesting adaptive diversification under ecological pressures. Structural analysis showed that the majority of ERF/RAV subfamily genes possess a single-exon structure, whereas AP2 subfamily genes display muti-exon structures, indicating divergent evolutionary trajectories and potential functional versatility via alternative splicing. Promoter analyses detected numerous hormone- and stress-responsive elements, linking these genes to abscisic acid, auxin, gibberellin signaling, and pathogen defense. Further expression profiling during stem development showed that approximately 60% of CoAP2/ERF genes were constitutively expressed across 17 expression trends, suggesting roles in basal development and stage-specific processes (e.g., lignification). Under Epicoccum poaceicola infection, 23 CoAP2/ERF genes were differentially expressed. Among them, upregulated ERF homologs related to RAP2.2/2.3 suggested roles in hypoxia and antimicrobial responses, while downregulation of ERF5 homologs indicated a growth–defense trade-off, whereby developmental processes are suppressed to prioritize pathogen resistance. Overall, this study deciphers the genomic architecture and structural diversity of CoAP2/ERF genes, along with expression dynamics of these genes in development and biotic stress adaptation of camphor trees. These findings provide critical insights into transcriptional regulation of development and stress responses in camphor trees and establish a theoretical basis for molecular breeding and biotechnological strategies aimed at improving stress resilience in woody plants. Full article

Catalpa bungei C.A.Mey is an economically significant deciduous tree valued for timber production and landscaping applications. An efficient regeneration system is crucial for clonal propagation and serves as a foundation for future molecular breeding in C. bungei. This study established two in vitro regeneration pathways—indirect somatic embryogenesis and shoot organogenesis utilizing mature zygotic embryos as explants. Primary callus was induced from cotyledon, hypocotyl, and plumule explants. A high frequency (45.73%) of yellow-green compact callus was achieved on De-Klerk and Walton (DKW) medium supplemented with 2.0 mg/L 6-BA, 1.0 mg/L zeatin (ZT), and 0.1 mg/L NAA. Subsequent transfer to 1.5× Murashige and Skoog (MS) medium containing 1.5 mg/L 6-BA, 0.2 mg/L ZT, and 0.1 mg/L NAA yielded the highest embryogenic callus induction rate (16.67%). Embryogenic callus demonstrated bipotent potential, generating both adventitious shoots and somatic embryos under specific hormonal conditions. Histological analyses confirmed the typical developmental stages of somatic embryos, from globular to cotyledonary forms, validating the embryogenic origin of regenerated structures. Furthermore, hormone or osmotic additives such as abscisic acid (ABA), Phytagel, and polyethylene glycol 4000 (PEG4000) significantly enhanced somatic embryo induction, with Phytagel at 5.0 g/L achieving the highest rate (76.31%). For shoot organogenesis, the optimal hormonal combination of the 0.6 mg/L 6-BA, 0.4 mg/L KT, and 0.15 mg/L NAA achieved the highest bud induction rate (88.89%) and produced an average of 4.07 adventitious buds per explant. This study presents an efficient regeneration system for C. bungei, providing a practical platform for large-scale propagation and basis for biotechnological applications in woody plants. Full article

Plants have the ability to perceive a wide range of light spectra, from which they derive not only the energy required for photosynthesis but also a variety of environmental cues and signals mediated by specific photoreceptors that trigger a cascade of biochemical reactions essential for their development. The olive tree (Olea europaea L.) is a woody species for which, despite its agronomic and economic relevance, the influence of light on its development remains poorly understood. The present study, a combined approach was employed, involving the phenotyping of 10 different cultivars exposed exclusively to red light (RL) and blue light (BL) for a period of two months, in addition to the monitoring of expression profiles of 10 photoreceptor-encoding genes in two of the cultivars that exhibited the most contrasting responses to the different light conditions. Our results revealed a correlation between the expression of specific genes and the differential response to exclusive exposure to the two light spectra, highlighting a generally enhanced photosynthetic activity of nearly all cultivars to blue light (BL) and, conversely, a negative response to red light (RL). Taken together, our data, by elucidating the response of the olive to specific light spectra and the underlying molecular mechanisms, pave the way for further studies on these traits, which could be useful for the improvement of this species. Full article

Drought stress poses a significant threat to tree growth, making the development of drought-resistant species essential for ecological restoration. WRKY transcription factors are critical regulators of plant drought responses; however, the role of WRKY22 in the woody species Populus ussuriensis K. remains unclear. In this study, the PuWRKY22 gene was cloned from P. ussuriensis via homologous cloning and was found to be highly expressed in leaves and responsive to abscisic acid (ABA) signaling. Subcellular localization confirmed that PuWRKY22 is a nuclear protein. Using fluorescein enzyme complementation assays, PuWRKY22 was shown to bind specifically to W-box cis-elements, indicating its function as a transcriptional regulator. Under ABA and osmotic (sorbitol) stress, the seed germination rate, root growth, and biomass of tobacco and Populus davidiana × Populus bolleana strains overexpressing PuWRKY22 were significantly increased. Additionally, these overexpressed strains exhibited a reduction in reactive oxygen species (ROS) accumulation and a decrease in membrane lipid peroxidation. Transcriptomic analyses revealed that PuWRKY22 activates expression of the ABA receptor gene Ptr.PYL4 (Potri.006G104100.v4.1), which regulates stomatal closure to minimize water loss. Consistent with this, stomatal observations and photosynthetic measurements demonstrated that PuWRKY22 enhances drought tolerance by protecting photosystem II and preserving chlorophyll content. Collectively, this study elucidates the molecular mechanism by which PuWRKY22 enhances drought resistance in woody plants through ABA signaling, providing a foundation for breeding drought-tolerant forest species. Full article

Walnut anthracnose (Colletotrichum gloeosporioides and C. siamense) and brown spot (Alternaria alternata) cause severe yield losses globally. Conventional fungicides face the challenges of pathogen resistance and environmental toxicity. This study evaluates 4-propylphenol, a plant-derived phenolic compound, as an eco-friendly alternative against key fungal pathogens of walnut. In vitro assays determined EC₅₀ values against target pathogens (29.11–31.89 mg·L⁻¹) via mycelial growth inhibition and conidial germination suppression (EC₅₀ = 55.04–71.85 mg·L⁻¹). Mechanistic analyses confirmed membrane disruption through propidium iodide staining (9.5-to-14.0-fold fluorescence intensity increase), DNA leakage (77.82–85.15% at 250 mg·L⁻¹), and protein efflux (58.10–66.49%). In field trials, we implemented a phenology-driven strategy: 100 mg·L⁻¹ ground/canopy spray at flowering to reduce primary inoculum, followed by 400 mg·L⁻¹ canopy application at fruiting. This protocol achieved 86.67% control efficacy against disease complexes with negligible phytotoxicity (SPAD variation < 5%). 4-propylphenol provides a sustainable solution through membrane-targeting action, effectively overcoming fungicide resistance in woody crops. Full article

Rosa canina is one of the precious native rose rootstocks with a high reputation among plant producers, which has potential horticultural and pharmacological properties related to the cosmetic values and the production of secondary metabolites. Due to high horticultural consumption, applying the plant tissue culture technique as a major tool for healthy and massive-scale production of R. canina plants is not unexpected. However, the response of R. canina in vitro plantlets to various plant tissue culture ingredients is not well understood to tender an efficient applied protocol for qualitative and quantitative in vitro propagation. In this regard, the main objective of this study is to investigate the influence of several abiotic in vitro variants including six plant tissue culture media formulations (McCown’s Woody Plant Medium (WPM), Murashige and Skoog (MS), Van der Salm (VS), Schenk and Hildebrant (SH), Driver Kuniyuki Walnut (DKW), and Gamburg B5 (B5)) in combination with four concentrations (0, 1.5, 3, 4 mgL⁻¹) of two types of cytokinins (6-Benzyaminopurine (BAP) and Kinetin (Kin)) simultaneously. Notably, it is perceived that DKW culture medium containing 1.5 mgL⁻¹ BAP and 0.1 mgL⁻¹ NAA is the best treatment for both in vitro morphological and flowering properties. Full article

The timing of male meiosis plays a pivotal role in ensuring successful pollination and may represent a critical window during which environmental stressors can significantly impact reproductive outcomes. In anemophilous plants, both the quantity of microspores produced and the development of viable pollen are particularly susceptible to external influences, such as fluctuating climatic conditions. This study undertakes a comprehensive analysis of reproductive features, focusing on the meiotic events of male gametogenesis and the phenological phases from the onset to full flowering in olive from central Italy. Utilizing a robust 11-year database (2012–2022), the research incorporates detailed micro- and macro-phenological observations alongside systematic pollen monitoring. The temporal regulation of male meiosis directs the phenological plasticity of the olive tree (Olea europaea L.) by transforming it into maladaptive phenological plasticity, effectively making the plant insensitive to thermal changes. This remarkable physiological trait underscores the resilience of this woody species to climate change. The results obtained will help to elucidate the interaction between climatic factors and reproductive dynamics, contributing valuable insights into the broader context of phenological responses to environmental changes. Full article

Paeonia ostii is an economically significant species serving as an ornamental, medicinal herb, and woody oilseed crop. Gene function elucidation and molecular breeding are hindered by the lack of efficient, stable transformation methods due to tissue culture challenges. To enable year-round functional studies without material constraints, we established a novel transient transformation system mediated by Agrobacterium using in vitro embryo-derived seedlings (TTAES) in P. ostii. By optimizing embryo germination media, we achieved consistent seedling production. Orthogonal experiments with a GUS reporter identified optimal conditions: OD₆₀₀ = 1.0, 200 μM of acetosyringone, six negative-pressure treatments, and 2 h infection. Under this optimized system, maximum transformation efficiency was achieved at 35 days after germination. With this system, we demonstrated its application in investigating transcription factor-mediated regulation of target gene promoters using GUS as a reporter gene. To achieve non-destructive identification of transiently transformed plants, we employed GFP as a reporter gene. Using transient expression of VIGS (knockdown) and 35S constructs (overexpression), we characterized gene functions, thereby confirming the system’s effectiveness for functional analysis. This system facilitates the acquisition of plant experimental materials and significantly improves research efficiency for year-round gene function elucidation in P. ostii. Full article

Chimonanthus praecox, a traditional ornamental plant in China, is admired for its ability to bloom during the cold winter season and is recognized as an outstanding woody cut flower. MADS-box genes encode transcription factors essential for plant growth and development, with key functions in regulating flowering time and the formation of floral organs. In this study, 74 MADS-box genes (CpMADS1–CpMADS74) were identified and mapped across 11 chromosomes, with chromosome 1 harboring the highest number (13 genes) and chromosome 3 the fewest (3 genes). Physicochemical property analysis revealed that all CpMADS proteins are hydrophilic and predominantly nuclear-localized. Phylogenetic analysis classified these genes into Type I and Type II subfamilies, highlighting a clear divergence in domain structure. Eighty simple sequence repeat (SSR) loci were detected, with dinucleotide repeats being the most abundant, and the majority located in Type II MADS genes. From 23 C. praecox samples, 10 polymorphic SSR markers were successfully developed and PCR-validated, enabling a cluster analysis that grouped these cultivars into three distinct clusters. This study offers significant insights into the regulation of flowering, floral organ development, genetic linkage map construction, and the application of marker-assisted selection in C. praecox. Full article

Fungi contribute to ecosystem function through nutrient cycling and decomposition but may be affected by major disturbances such as fire. Some ecosystems are fire-adapted, such as prairies which require cyclical burning to mitigate woody plant encroachment and reduce litter. While fire suppresses fire-sensitive fungi, pyrophilous fungi may continue providing ecosystem functions. Using litter bags, we measured the litter decomposition at three prairies with unburned and burned sections, and we used Illumina sequencing to examine litter communities. We hypothesized that (H1) decomposition would be higher at unburned sites than burned, (H2) increased decomposition at unburned sites would be correlated with higher overall saprotroph diversity, with a lower diversity in autoclaved samples, and (H3) pyrophilous fungal diversity would be higher at burned sites and overall higher in autoclaved samples. H1 was not supported; decomposition was unaffected by burn treatments. H2 and H3 were somewhat supported; saprotroph diversity was lowest in autoclaved litter at burned sites, but pyrophilous fungal diversity was the highest. Pyrophilous fungal diversity significantly contributed to litter decomposition rates, while saprotroph diversity did not. Our findings indicate that fire-adapted prairies host a suite of pyrophilous saprotrophic fungi, and that these fungi play a primary role in litter decomposition post-fire when other fire-sensitive fungal saprotrophs are less abundant. Full article