Spectroscopic Investigations of the Binding Interaction of a New Indanedione Derivative with Human and Bovine Serum Albumins
Abstract
:1. Introduction
2. Results and Discussion
2.1. Fluorescence quenching of serum albumins in presence of HEBID
2.2. Estimation of the binding parameters
K (M-1) ×10-4 | n | R | SD | ||
---|---|---|---|---|---|
Equation (2) | HSA | 6.54 | 0.96 | 0.9985 | 0.0279 |
BSA | 5.65 | 0.81 | 0.9989 | 0.0209 | |
Equation (3) | HSA | 5.47 | 0.99 | 0.9987 | 0.0262 |
BSA | 1.11 | 0.86 | 0.9992 | 0.0180 |
2.3. Effect of HEBID on the conformations of HSA and BSA
2.3.1 Synchronous fluorescence measurements
2.3.2. Circular dichroism results
2.4. Energy transfer efficiency and binding distance
E | J × 1015 (M-1cm3) | R0 (nm) | r (nm) | |
---|---|---|---|---|
HSA | 0.16 | 1.48 | 1.78 | 2.34 |
BSA | 0.18 | 1.51 | 1.79 | 2.31 |
3. Experimental
3.1. General
3.2. Synthesis
3.3. Procedures
4. Conclusions
References
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Stan, D.; Matei, I.; Mihailescu, C.; Savin, M.; Matache, M.; Hillebrand, M.; Baciu, I. Spectroscopic Investigations of the Binding Interaction of a New Indanedione Derivative with Human and Bovine Serum Albumins. Molecules 2009, 14, 1614-1626. https://doi.org/10.3390/molecules14041614
Stan D, Matei I, Mihailescu C, Savin M, Matache M, Hillebrand M, Baciu I. Spectroscopic Investigations of the Binding Interaction of a New Indanedione Derivative with Human and Bovine Serum Albumins. Molecules. 2009; 14(4):1614-1626. https://doi.org/10.3390/molecules14041614
Chicago/Turabian StyleStan, Dana, Iulia Matei, Carmen Mihailescu, Mihaela Savin, Mihaela Matache, Mihaela Hillebrand, and Ion Baciu. 2009. "Spectroscopic Investigations of the Binding Interaction of a New Indanedione Derivative with Human and Bovine Serum Albumins" Molecules 14, no. 4: 1614-1626. https://doi.org/10.3390/molecules14041614