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Molecules 2012, 17(12), 13825-13843; doi:10.3390/molecules171213825

Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays

1
Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), CIBER-BBN Networking Centre on Bioengineering, Biomaterials and Nanomedicine, Jordi Girona 18, E-08034 Barcelona, Spain
2
Institute for Research in Biomedicine (IRB Barcelona), Baldiri Reixac 10, E-08028 Barcelona, Spain
3
Unit of Synthesis & Biomedical Applications of Peptides, Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), Jordi Girona 18, E-08034 Barcelona, Spain
*
Author to whom correspondence should be addressed.
Received: 17 October 2012 / Revised: 16 November 2012 / Accepted: 16 November 2012 / Published: 22 November 2012
(This article belongs to the Special Issue Nucleic Acid Analogs)
View Full-Text   |   Download PDF [285 KB, 18 June 2014; original version 18 June 2014]   |  

Abstract

Here we used solid-phase methods to prepare oligonucleotides carrying fibrin/ filaggrin citrullinated peptides. Post-synthetic conjugation protocols were successfully applied for the synthesis of oligonucleotides carrying small peptides. A stepwise protocol using acid treatment for the final deprotection allowed the preparation of polypyrimidine oligonucleotides carrying longer and arginine-rich peptides. An ELISA-based test using the oligonucleotide-citrullinated peptide conjugates was developed for the detection of anti-citrullinated protein/peptide antibodies in human serum from rheumatoid arthritis patients. View Full-Text
Keywords: oligonucleotide-peptide conjugates; ELISA; solid-phase synthesis; rheumatoid arthritis; epitopes; anti-citrullinated protein/peptide antibodies; ACPA; fibrin; filaggrin; citrullinated peptides oligonucleotide-peptide conjugates; ELISA; solid-phase synthesis; rheumatoid arthritis; epitopes; anti-citrullinated protein/peptide antibodies; ACPA; fibrin; filaggrin; citrullinated peptides
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Aviñó, A.; Gómara, M.J.; Malakoutikhah, M.; Haro, I.; Eritja, R. Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays. Molecules 2012, 17, 13825-13843.

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