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Molecules 2013, 18(2), 2122-2134; doi:10.3390/molecules18022122

PF2401-SF, Standardized Fraction of Salvia miltiorrhiza, Induces Apoptosis of Activated Hepatic Stellate Cells in Vitro and in Vivo

Department of Pharmacy, Institute of Pharmaceutical Research and Development, Wonkwang University, Iksan, Jeonbuk 570-749, Korea
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Received: 28 December 2012 / Revised: 25 January 2013 / Accepted: 4 February 2013 / Published: 6 February 2013
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Abstract

During the course of our attempts to develop a potential herbal medicine, we had previously prepared PF2401-SF, a standardized fraction of S. miltiorrhiza, and reported its hepatoprotective activity in vitro as well as in vivo. Since apoptosis of activated hepatic stellate cells (HSCs) is a well-accepted anti-fibrotic strategy, in this study, we investigated the direct effect of PF2401-SF on t-HSC/Cl-6 cells in vitro and on CCl4-induced liver injury in vivo. We evaluated the activation and cleavage of hallmarkers of apoptosis, namely, caspase 3, 8, 9 and PARP. Upregulation of the pro-apoptotic Bax protein and downregulation of the anti-apoptotic Bcl2 protein were also analyzed. Furthermore, in the PF2401-SF treated rats, apoptosis induction of activated HSCs was demonstrated by reduced distribution of α-SMA-positive cells and the presence of high number of TUNEL-positive cells in vivo. Our data suggest that PF2401-SF can mediate HSCs apoptosis induction, and may be a potential herbal medicine for the treatment of liver fibrosis.
Keywords: Salvia miltiorrhiza; hepatic stellate cells; caspases; apoptosis Salvia miltiorrhiza; hepatic stellate cells; caspases; apoptosis
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Parajuli, D.R.; Park, E.-J.; Che, X.-H.; Jiang, W.-Y.; Kim, Y.-C.; Sohn, D.H.; Lee, S.H. PF2401-SF, Standardized Fraction of Salvia miltiorrhiza, Induces Apoptosis of Activated Hepatic Stellate Cells in Vitro and in Vivo. Molecules 2013, 18, 2122-2134.

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