Next Article in Journal
Quenching of Tryptophan Fluorescence in the Presence of 2,4-DNP, 2,6-DNP, 2,4-DNA and DNOC and Their Mechanism of Toxicity
Previous Article in Journal
Growth of Fullerene Fragments Using the Diels-Alder Cycloaddition Reaction: First Step towards a C60 Synthesis by Dimerization
Article Menu

Export Article

Open AccessArticle
Molecules 2013, 18(2), 2255-2265; doi:10.3390/molecules18022255

Characterization of Proanthocyanidins in Stems of Polygonum multiflorum Thunb as Strong Starch Hydrolase Inhibitors

Food Science and Technology Program, Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, 117543, Singapore
The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Chinese National Center of Plant Gene Research (Wuhan) HUST Part, College of Life Science and Technology, Huazhong University of Science & Technology, Luoyu Road 1037, Wuhan 430074, Hubei, China
Jing Brand Company, 169 Daye Avenue, Daye 435100, Hubei, China
Author to whom correspondence should be addressed.
Received: 10 December 2012 / Revised: 5 February 2013 / Accepted: 5 February 2013 / Published: 18 February 2013
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [385 KB, uploaded 18 June 2014]   |  


Characterzation of polyphenolic compounds in the stems of P. multiflorum was conducted using HPLC, high resolution LC-MS and LC-MSn. Proanthocyanidins in particular were isolated in 4.8% yield using solvent extraction followed by Sephadex LH-20 fractionation. HPLC analysis using a diol column revealed oligomers (from dimer to nonamer) as minor components, with (epi)catechin monomeric units predominating, and oligomers with higher degree of polymerization being dominant. Thiolysis treatment of the proanthocyanidins using mercaptoacetic acid produced thioether derivatives of (epi)catechin as the major product and a mean value of the degree of polymerization of 32.6 was estimated from the ratio of terminal and extension units of the (epi)catechin. The isolated proanthocyanidins were shown to strongly inhibit α-amylase with an acarbose equivalence (AE) value of 1,954.7 µmol AE/g and inhibit α-glucosidase with an AE value of 211.1 µmol AE/g.
Keywords: P. multiflorum; proanthocyanidins; α-amylase; (epi)catechin P. multiflorum; proanthocyanidins; α-amylase; (epi)catechin
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Wang, H.; Song, L.; Feng, S.; Liu, Y.; Zuo, G.; Lai, F.; He, G.; Chen, M.; Huang, D. Characterization of Proanthocyanidins in Stems of Polygonum multiflorum Thunb as Strong Starch Hydrolase Inhibitors. Molecules 2013, 18, 2255-2265.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top