Ethanol Extract of Atractylodes macrocephala Protects Bone Loss by Inhibiting Osteoclast Differentiation
Abstract
:1. Introduction
2. Results and Discussion
2.1. AMEE Inhibits Osteoclast Differentiation in Bone Marrow Cell-Osteoblast Coculture
2.2. AMEE Inhibits RANKL-Induced Osteoclastogenesis from Osteoclast Precursors
2.3. AMEE Inhibits RANKL-Induced c-Fos and NFATc1 Expression in Osteoclast Precursors
2.4. AMEE Attenuates RANKL-Induced NF-κB Activation in Osteoclast Precursors
2.5. AMEE Attenuates RANKL-Induced Bone Loss in Mice
2.6. Atractylenolide I Inhibits RANKL-Induced Osteoclast Differentiation
3. Experimental
3.1. Reagents and Antibodies
3.2. Preparation of AMEE
3.3. Cell Culture
3.4. TRAP Activity and Staining
3.5. QPCR Analysis
3.6. Western Blot Analysis
3.7. Animal Experiments and Micro-CT Analysis
3.8. HPLC Analysis
3.9. Statistical Analysis
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Ha, H.; An, H.; Shim, K.-S.; Kim, T.; Lee, K.J.; Hwang, Y.-H.; Ma, J.Y. Ethanol Extract of Atractylodes macrocephala Protects Bone Loss by Inhibiting Osteoclast Differentiation. Molecules 2013, 18, 7376-7388. https://doi.org/10.3390/molecules18077376
Ha H, An H, Shim K-S, Kim T, Lee KJ, Hwang Y-H, Ma JY. Ethanol Extract of Atractylodes macrocephala Protects Bone Loss by Inhibiting Osteoclast Differentiation. Molecules. 2013; 18(7):7376-7388. https://doi.org/10.3390/molecules18077376
Chicago/Turabian StyleHa, Hyunil, Hyosun An, Ki-Shuk Shim, Taesoo Kim, Kwang Jin Lee, Youn-Hwan Hwang, and Jin Yeul Ma. 2013. "Ethanol Extract of Atractylodes macrocephala Protects Bone Loss by Inhibiting Osteoclast Differentiation" Molecules 18, no. 7: 7376-7388. https://doi.org/10.3390/molecules18077376