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Molecules 2013, 18(8), 8786-8798; doi:10.3390/molecules18088786

Isoliquiritigen Enhances the Antitumour Activity and Decreases the Genotoxic Effect of Cyclophosphamide

1,†, 2,†, 1, 1, 1, 2, 3,*  and 1,4,*
1 School of Pharmacy, Shihezi University, Shihezi 832002, China 2 Lanzhou University Second Hospital, Lanzhou University, Lanzhou 730000, China 3 School of integrated traditional Chinese and Western Medicine, Binzhou Medical College, Yantai 264000, China 4 Life Science School, Yantai University, Yantai 264000, China These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 3 May 2013 / Revised: 16 July 2013 / Accepted: 22 July 2013 / Published: 24 July 2013
(This article belongs to the Section Natural Products)
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The aim of this study was to evaluate the antitumour activities and genotoxic effects of isoliquiritigenin (ISL) combined with cyclophosphamide (CP) in vitro and in vivo. U14 cells were treated with either of ISL (5–25 μg/mL) or CP (0.25–1.25 mg/mL) alone or with combination of ISL (5–25 μg/mL) and CP (1.0 mg/mL) for 48 h. The proliferation inhibitory effect in vitro was evaluated by MTT and colony formation assays. KM mice bearing U14 mouse cervical cancer cells were used to estimate the antitumour activity in vivo. The genotoxic activity in bone marrow polychromatic erythrocytes was assayed by frequency of micronuclei. The DNA damage in peripheral white blood cells was assayed by single cell gel electrophoresis. The results showed that ISL enhanced antitumour activity of CP in vitro and in vivo, and decreased the micronucleus formation in polychromatic erythrocytes and DNA strand breaks in white blood cells in a dose-dependent way.
Keywords: isoliquiritigenin; antitumour; cyclophosphamide; genotoxic effect isoliquiritigenin; antitumour; cyclophosphamide; genotoxic effect
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Zhao, H.; Yuan, X.; Li, D.; Chen, H.; Jiang, J.; Wang, Z.; Sun, X.; Zheng, Q. Isoliquiritigen Enhances the Antitumour Activity and Decreases the Genotoxic Effect of Cyclophosphamide. Molecules 2013, 18, 8786-8798.

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