Antidiabetic and Antioxidant Properties of Alkaloids from Catharanthus roseus (L.) G. Don
Abstract
:1. Introduction
2. Results and Discussion
2.1. Results
2.1.1. Identification of the Alkaloid Constituents
2.1.2. Effect of Alkaloids on β-TC6 Cell Viability
Cytotoxicity (IC50) | DE | Vindoline | Vindolidine | Vindolicine | Vindolinine |
---|---|---|---|---|---|
µg/mL | 78.4 ± 12.2 | 82.1 ± 9.8 | 76.7 ± 8.1 | 68.0 ± 10.4 | 20.5 ± 3.6 |
µM | - | 180.1 ± 21.5 | 180.1 ± 19.0 | 73.5 ± 11.3 | 57.6 ± 10.7 |
2.1.3. ORAC Evaluation
2.1.4. DPPH Assay
2.1.5. Effect of Alkaloids on H2O2-induced ROS Production in β-TC6 Cells
2.1.6. Effect of Alkaloid on Glucose Uptake in β-TC6 and C2C12 Cells
2.1.7. Effect of Alkaloid on PTP-1B Inhibition
2.2. Discussion
3. Experimental
3.1. Plant Material
3.2. Extraction and Fractionation
3.3. Isolation and Purification
3.4. Identification and Characterization of Alkaloids
3.5. Cell Culture
3.6. Cellular Viability
3.7. ORAC Assay
3.8. DPPH Assay
3.9. Intracellular ROS Measurement
3.11. PTP-1B Inhibition
3.12. Statistical Analysis
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Tiong, S.H.; Looi, C.Y.; Hazni, H.; Arya, A.; Paydar, M.; Wong, W.F.; Cheah, S.-C.; Mustafa, M.R.; Awang, K. Antidiabetic and Antioxidant Properties of Alkaloids from Catharanthus roseus (L.) G. Don. Molecules 2013, 18, 9770-9784. https://doi.org/10.3390/molecules18089770
Tiong SH, Looi CY, Hazni H, Arya A, Paydar M, Wong WF, Cheah S-C, Mustafa MR, Awang K. Antidiabetic and Antioxidant Properties of Alkaloids from Catharanthus roseus (L.) G. Don. Molecules. 2013; 18(8):9770-9784. https://doi.org/10.3390/molecules18089770
Chicago/Turabian StyleTiong, Soon Huat, Chung Yeng Looi, Hazrina Hazni, Aditya Arya, Mohammadjavad Paydar, Won Fen Wong, Shiau-Chuen Cheah, Mohd Rais Mustafa, and Khalijah Awang. 2013. "Antidiabetic and Antioxidant Properties of Alkaloids from Catharanthus roseus (L.) G. Don" Molecules 18, no. 8: 9770-9784. https://doi.org/10.3390/molecules18089770