Rapid Identification of Antioxidant Compounds of Genista saharae Coss. & Dur. by Combination of DPPH Scavenging Assay and HPTLC-MS
Abstract
:1. Introduction
2. Results and Discussion
Sample | Part | Harvesting period | Extraction Yield (%) | % Flavonoids content (SD) | % Total phenolic content (SD) |
---|---|---|---|---|---|
1 | Roots | August 2009 | 16.31 | 2,441(0,012) | 9,328(0,217) |
2 | Roots | March 2010 | 8.78 | 0,212(0,014) | 5,490(0,198) |
3 | Stems | August 2009 | 10.93 | 0,213(0,022) | 5,799(0,148) |
4 | Stems | March 2010 | 15.07 | 0,924(0,012) | 6,809(0,106) |
5 | Flowers | March 2010 | 18.00 | 2,614(0,028) | 9,067(0,144) |
6 | Fruit pods | August 2009 | 6.69 | 1,273(0,012) | 5,062(0,163) |
7 | Fruit Seeds | August 2009 | 7.70 | 0,518(0,016) | 1,996(0,163) |
Spot numbers | m/z values [M−H]− | Putative structures |
---|---|---|
A1 | 299.2 | - |
A2 | 285.1 | 3',4',5,7-Tetrahydroxyisoflavone (1) |
A3 | 351.2 | Hydroxyalpinumisoflavone (2) |
A4 | 465.2 | - |
A5 | 285.1/353.1 | Tetrahydroxyisoflavone (1)/ficuisoflavone (3) |
B1 | - | - |
B2 | 445.2 | 5-Methoxy-4',7-trihydroxy-8-glucopyranosyl (4) |
B3 | 445.2 | 4',5-Dihydroxy-7-methoxyisoflavone-4'-O-β-d-glucopyranoside (5) |
B4 | 283.1 | 4',5-Dihydroxy-7-methoxyisoflavone (6) |
B5 | 445.2/575.6/325.2 | - |
2.1. Identification of Compound 4
2.2. Radical Scavenging Capacity (DPPH)
3. Experimental
3.1. General
3.2. Plant Material
3.3. Extraction
3.4. Total Polyphenol and Flavonoids Dosage
3.4.1. Total Phenolic Content
3.4.2. Flavonoids Content
3.5. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) Radical Scavenging Capacity Assay
3.6. HPTLC-DPPH Scavenging Assay
3.7. HPTLC-MS
3.8. Purification of Compound 4
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Meriane, D.; Genta-Jouve, G.; Kaabeche, M.; Michel, S.; Boutefnouchet, S. Rapid Identification of Antioxidant Compounds of Genista saharae Coss. & Dur. by Combination of DPPH Scavenging Assay and HPTLC-MS. Molecules 2014, 19, 4369-4379. https://doi.org/10.3390/molecules19044369
Meriane D, Genta-Jouve G, Kaabeche M, Michel S, Boutefnouchet S. Rapid Identification of Antioxidant Compounds of Genista saharae Coss. & Dur. by Combination of DPPH Scavenging Assay and HPTLC-MS. Molecules. 2014; 19(4):4369-4379. https://doi.org/10.3390/molecules19044369
Chicago/Turabian StyleMeriane, Djamila, Grégory Genta-Jouve, Mohamed Kaabeche, Sylvie Michel, and Sabrina Boutefnouchet. 2014. "Rapid Identification of Antioxidant Compounds of Genista saharae Coss. & Dur. by Combination of DPPH Scavenging Assay and HPTLC-MS" Molecules 19, no. 4: 4369-4379. https://doi.org/10.3390/molecules19044369
APA StyleMeriane, D., Genta-Jouve, G., Kaabeche, M., Michel, S., & Boutefnouchet, S. (2014). Rapid Identification of Antioxidant Compounds of Genista saharae Coss. & Dur. by Combination of DPPH Scavenging Assay and HPTLC-MS. Molecules, 19(4), 4369-4379. https://doi.org/10.3390/molecules19044369