Algae Undaria pinnatifida Protects Hypothalamic Neurons against Endoplasmic Reticulum Stress through Akt/mTOR Signaling
Abstract
:1. Introduction
2. Results
2.1. UP Has a Protective Effect in Tunicamycin-Induced Cell Death
2.2. UP Attenuates ER Stress
2.3. UP Attenuates ER Stress Induced Apoptotic Cell Death
2.4. UP Increases Cell Viability via Akt/mTOR Signaling
3. Discussion
4. Experimental Section
4.1. Reagents and Cells
4.2. Preparation of Undaria pinnatifida Extract
4.3. Cell Viability Test
4.4. Transient Transfection with siRNA
Target Genes | Symbols | GenBank No. | siRNA Sequences | |
---|---|---|---|---|
Control siRNA | Forward, 5’-ACGUGACACGUUCGGAGAAUU-3’ Reverse, 5’-UUCUCCGAACGUGUCACGUUU-3’ | |||
Thymoma viral proto-oncogene (Akt) | ||||
Thymoma viral proto-oncogene | akt | NM_009652 | #1 | Forward, 5’-GAACGAUGGCACCUUUAUUUU-3’ Reverse, 5’-AAUAAAGGUGCCAUCGUUCUU-3’ |
#2 | Forward,5’-GGUUCUUUGCCAACAUCGUUU-3’ Reverse, 5’-ACGAUGUUGGCAAAGAACCUU-3’ |
4.5. Reverse Transcription-PCR
4.6. Western Blot Analysis
4.7. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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- Sample Availability: Samples of the compounds are not available from the authors.
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Kim, J.; Moon, I.S.; Goo, T.-W.; Moon, S.-S.; Seo, M. Algae Undaria pinnatifida Protects Hypothalamic Neurons against Endoplasmic Reticulum Stress through Akt/mTOR Signaling. Molecules 2015, 20, 20998-21009. https://doi.org/10.3390/molecules201219744
Kim J, Moon IS, Goo T-W, Moon S-S, Seo M. Algae Undaria pinnatifida Protects Hypothalamic Neurons against Endoplasmic Reticulum Stress through Akt/mTOR Signaling. Molecules. 2015; 20(12):20998-21009. https://doi.org/10.3390/molecules201219744
Chicago/Turabian StyleKim, Jongwan, Il Soo Moon, Tae-Won Goo, Seong-Su Moon, and Minchul Seo. 2015. "Algae Undaria pinnatifida Protects Hypothalamic Neurons against Endoplasmic Reticulum Stress through Akt/mTOR Signaling" Molecules 20, no. 12: 20998-21009. https://doi.org/10.3390/molecules201219744