The Influence of Sesquiterpenes from Myrica rubra on the Antiproliferative and Pro-Oxidative Effects of Doxorubicin and Its Accumulation in Cancer Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. Effect of Individual Sesquiterpenes on Cell Proliferation/Viability
IC50 (µg/mL) | |
---|---|
CAR | - |
HUM | 24.4 ± 2.4 |
VAL | 38.1 ± 2.4 |
CAO | 57.7 ± 3.9 |
NER | 28.7 ± 2.5 |
2.2. Effect of Sesquiterpenes in Combinations with Doxorubicin
3. Experimental Section
3.1. Chemicals and Reagents
3.2. Cancer Cell Culture
3.3. Preparation of Primary Culture of Rat Hepatocytes
3.4. Tests of Cell Viability
3.4.1. The Neutral Red Uptake (NRU) Test
3.4.2. The MTT Assay
3.5. Determination of Intracellular Reactive Oxygen Species (ROS) Formation
3.6. Test of DOX Accumulation in Cells
3.7. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Ambrož, M.; Boušová, I.; Skarka, A.; Hanušová, V.; Králová, V.; Matoušková, P.; Szotáková, B.; Skálová, L. The Influence of Sesquiterpenes from Myrica rubra on the Antiproliferative and Pro-Oxidative Effects of Doxorubicin and Its Accumulation in Cancer Cells. Molecules 2015, 20, 15343-15358. https://doi.org/10.3390/molecules200815343
Ambrož M, Boušová I, Skarka A, Hanušová V, Králová V, Matoušková P, Szotáková B, Skálová L. The Influence of Sesquiterpenes from Myrica rubra on the Antiproliferative and Pro-Oxidative Effects of Doxorubicin and Its Accumulation in Cancer Cells. Molecules. 2015; 20(8):15343-15358. https://doi.org/10.3390/molecules200815343
Chicago/Turabian StyleAmbrož, Martin, Iva Boušová, Adam Skarka, Veronika Hanušová, Věra Králová, Petra Matoušková, Barbora Szotáková, and Lenka Skálová. 2015. "The Influence of Sesquiterpenes from Myrica rubra on the Antiproliferative and Pro-Oxidative Effects of Doxorubicin and Its Accumulation in Cancer Cells" Molecules 20, no. 8: 15343-15358. https://doi.org/10.3390/molecules200815343
APA StyleAmbrož, M., Boušová, I., Skarka, A., Hanušová, V., Králová, V., Matoušková, P., Szotáková, B., & Skálová, L. (2015). The Influence of Sesquiterpenes from Myrica rubra on the Antiproliferative and Pro-Oxidative Effects of Doxorubicin and Its Accumulation in Cancer Cells. Molecules, 20(8), 15343-15358. https://doi.org/10.3390/molecules200815343