Antibacterial Activity of 7-Epiclusianone and Its Novel Copper Metal Complex on Streptococcus spp. Isolated from Bovine Mastitis and Their Cytotoxicity in MAC-T Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. Chemistry
2.2. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC)
2.3. Time-Kill Curve
2.4. Protein Leakage Assay
2.5. Adherence Assessment
2.6. Cytotoxic Effect
3. Materials and Methods
3.1. Chemistry, Strains, and Culture Medium
3.1.1. Instrumentation General Methods
3.1.2. 7-Epiclusianone-Cu Physical Data
3.2. Minimum Inhibitory Concentration (MIC)
3.3. Minimum Bactericidal Concentration (MBC)
3.4. Time Kill Curve
3.5. Protein Leakage Assay
3.6. Adherence Assessment
3.7. Cytotoxic Effect in MAC-T
3.8. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Samples of the compounds 7-epiclusianone and 7-epiclusianone-copper are available from the authors. |
Strains | MIC | MBC | ||
---|---|---|---|---|
Compounds (µg mL−1/µM) | ||||
7-epi | 7-epi-Cu | 7-epi | 7-epi-Cu | |
SA3930 | - | - | - | - |
SA4038 | 7.8/15.5 | 7.8/7.3 | 15.6/31.1 | 31.3/29.3 |
SU959 | 7.8/15.5 | 7.8/7.3 | 31.3/62.2 | 31.3/29.3 |
SU3580 | 7.8/15.5 | 7.8/7.3 | 31.3/62.2 | 31.3/29.3 |
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De Barros, M.; Perciano, P.G.; Dos Santos, M.H.; De Oliveira, L.L.; Costa, É.D.; Moreira, M.A.S. Antibacterial Activity of 7-Epiclusianone and Its Novel Copper Metal Complex on Streptococcus spp. Isolated from Bovine Mastitis and Their Cytotoxicity in MAC-T Cells. Molecules 2017, 22, 823. https://doi.org/10.3390/molecules22050823
De Barros M, Perciano PG, Dos Santos MH, De Oliveira LL, Costa ÉD, Moreira MAS. Antibacterial Activity of 7-Epiclusianone and Its Novel Copper Metal Complex on Streptococcus spp. Isolated from Bovine Mastitis and Their Cytotoxicity in MAC-T Cells. Molecules. 2017; 22(5):823. https://doi.org/10.3390/molecules22050823
Chicago/Turabian StyleDe Barros, Mariana, Pedro Griffo Perciano, Marcelo Henrique Dos Santos, Leandro Licursi De Oliveira, Éderson D’Martin Costa, and Maria Aparecida Scatamburlo Moreira. 2017. "Antibacterial Activity of 7-Epiclusianone and Its Novel Copper Metal Complex on Streptococcus spp. Isolated from Bovine Mastitis and Their Cytotoxicity in MAC-T Cells" Molecules 22, no. 5: 823. https://doi.org/10.3390/molecules22050823