Molecules 2017, 22(5), 843; doi:10.3390/molecules22050843
Rapidly Simultaneous Determination of Six Effective Components in Cistanche tubulosa by Near Infrared Spectroscopy
College of Agronomy, China Agricultural University, Yuanming Yuan West Road, Haidian District, Beijing 100193, China
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Academic Editor: Derek McPhee
Received: 11 April 2017 / Revised: 6 May 2017 / Accepted: 16 May 2017 / Published: 19 May 2017
Abstract
Quantitative determination of multiple effective components in a given plant usually requires a very large amount of authentic natural products. In this study, we proposed a rapid and non-destructive method for the simultaneous determination of echinacoside, verbascoside, mannitol, sucrose, glucose and fructose in Cistanche tubulosa by near infrared spectroscopy (NIRS). Near infrared diffuse reflectance spectroscopy (DRS) and high performance liquid chromatography (HPLC) were conducted on 116 batches of C. tubulosa samples. The DRS data were processed using standard normal variety (SNV) and multiplicative scatter correction (MSC) methods. Partial least squares regression (PLSR) was utilized to build calibration models for components-of-interest in C. tubulosa. All models were then assessed by calculating the root mean square error of calibration (RMSEC), correlation coefficient of calibration (r). The r values of all six calibration models were determined to be greater than 0.94, suggesting each model is reliable. Therefore, the quantitative NIR models reported in this study can be qualified to accurately quantify the contents of six medicinal components in C. tubulosa. View Full-TextKeywords:
Cistanche tubulosa; high performance liquid chromatography; near infrared spectroscopy; partial least squares
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Wang, X.; Wang, X.; Guo, Y. Rapidly Simultaneous Determination of Six Effective Components in Cistanche tubulosa by Near Infrared Spectroscopy. Molecules 2017, 22, 843.
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