Immunosuppressive Effect of Geniposide on Mitogen-Activated Protein Kinase Signalling Pathway and Their Cross-Talk in Fibroblast-Like Synoviocytes of Adjuvant Arthritis Rats
Abstract
:1. Introduction
2. Results
2.1. Evaluation of Arthritis on AA Rats
2.2. The Effects of GE on the Joint Histopathology of AA Rats
2.3. The Effect of GE on FLS Viability
2.4. Effects of GE on the Proliferation of FLS
2.4.1. Effects of GE on the Proliferation of FLS In Vivo
2.4.2. Effects of GE on the Proliferation of FLS In Vitro
2.5. Effects of GE on Production of IFN-γ, IL-17, IL-4, and TGF-β1 in FLS
2.5.1. Effects of GE on Production of IFN-γ, IL-17, IL-4, and TGF-β1 in FLS In Vivo
2.5.2. Effects of GE on Production of IFN-γ, IL-17, IL-4, and TGF-β1 in FLS In Vitro
2.6. Effects of GE on MAPK Pathways and Cross-Talk among MAPK Pathways in FLS
2.6.1. Effects of GE on MAPK Pathways and Cross-Talk among MAPK Pathways in FLS In Vivo
2.6.2. Effects of GE on MAPK Pathways and Cross-Talk among MAPK Pathways in FLS In Vitro
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Reagents
4.3. Experimental Design
4.4. Arthritis Assessment
4.5. Histopathological Analysis
4.6. CCK8 Assay for FLS Viability
4.7. Proliferation Assay by MTT
4.8. Measurement of IFN-γ, IL-17, IL-4, and TGF-β1
4.9. Western Blot Analysis
4.9.1. Separation of Key Proteins from FLS In Vivo and In Vitro
4.9.2. Western Blotting
4.10. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are available from the authors. |
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Li, F.; Dai, M.; Wu, H.; Deng, R.; Fu, J.; Zhang, Z.; Dai, L.; Wang, W.; Dai, X.; Zhan, X.; et al. Immunosuppressive Effect of Geniposide on Mitogen-Activated Protein Kinase Signalling Pathway and Their Cross-Talk in Fibroblast-Like Synoviocytes of Adjuvant Arthritis Rats. Molecules 2018, 23, 91. https://doi.org/10.3390/molecules23010091
Li F, Dai M, Wu H, Deng R, Fu J, Zhang Z, Dai L, Wang W, Dai X, Zhan X, et al. Immunosuppressive Effect of Geniposide on Mitogen-Activated Protein Kinase Signalling Pathway and Their Cross-Talk in Fibroblast-Like Synoviocytes of Adjuvant Arthritis Rats. Molecules. 2018; 23(1):91. https://doi.org/10.3390/molecules23010091
Chicago/Turabian StyleLi, Feng, Miaomiao Dai, Hong Wu, Ran Deng, Jun Fu, Zhengrong Zhang, Li Dai, Wenyu Wang, Xuejing Dai, Xiang Zhan, and et al. 2018. "Immunosuppressive Effect of Geniposide on Mitogen-Activated Protein Kinase Signalling Pathway and Their Cross-Talk in Fibroblast-Like Synoviocytes of Adjuvant Arthritis Rats" Molecules 23, no. 1: 91. https://doi.org/10.3390/molecules23010091