Salvia castanea Hairy Roots are More Tolerant to Phosphate Deficiency than Salvia miltiorrhiza Hairy Roots Based on the Secondary Metabolism and Antioxidant Defenses
Abstract
:1. Introduction
2. Results and Discussion
2.1. Biomass of Hairy Roots under Different Pi Concentrations
2.2. Effects of Pi Deficiency on the Content and Yields of Specialized Metabolites in Hairy Roots
2.3. Effects of Pi Deficiency on the Quantification of the Transcript Levels of Key Enzyme Genes in Hairy Roots
2.4. Expression Response of the SPX Genes to Pi Deficiency
2.5. Pi Accumulation in Hairy Roots at Control and Pi Deficiency Conditions
2.6. Antioxidant Enzyme Responses to Pi Deficiency
2.7. Effects of Pi Deficiency on the Antioxidant Activity of Salvia Hairy Roots
3. Materials and Methods
3.1. Plant Material and Pi Deficiency Treatment
3.2. High-Performance Liquid Chromatography (HPLC)
3.3. RNA Extraction, cDNA Synthesis, and Quantitative Real-Time PCR (qRT-PCR) Assays
3.4. Measurement of Effective Pi Concentration
3.5. Analysis of Enzyme Activities
3.6. DPPH and ABTS+ Radical Cation Screening Assay
3.7. Data Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are available from the authors. |
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Liu, L.; Yang, D.; Xing, B.; Zhang, H.; Liang, Z. Salvia castanea Hairy Roots are More Tolerant to Phosphate Deficiency than Salvia miltiorrhiza Hairy Roots Based on the Secondary Metabolism and Antioxidant Defenses. Molecules 2018, 23, 1132. https://doi.org/10.3390/molecules23051132
Liu L, Yang D, Xing B, Zhang H, Liang Z. Salvia castanea Hairy Roots are More Tolerant to Phosphate Deficiency than Salvia miltiorrhiza Hairy Roots Based on the Secondary Metabolism and Antioxidant Defenses. Molecules. 2018; 23(5):1132. https://doi.org/10.3390/molecules23051132
Chicago/Turabian StyleLiu, Lin, Dongfeng Yang, Bingcong Xing, Haihua Zhang, and Zongsuo Liang. 2018. "Salvia castanea Hairy Roots are More Tolerant to Phosphate Deficiency than Salvia miltiorrhiza Hairy Roots Based on the Secondary Metabolism and Antioxidant Defenses" Molecules 23, no. 5: 1132. https://doi.org/10.3390/molecules23051132