Impact of Different Storage Methods on Bioactive Compounds in Arthrospira platensis Biomass
Abstract
:1. Introduction
2. Results and Discussion
2.1. The Monitoring of A. platensis Cultivation
2.2. Influence of Different Storage Methods on Photosynthetic Pigments of A. platensis
2.3. Total Proteins and Antioxidant Molecules in A. platensis Samples
2.4. Phytochemical Screening and Antioxidant Activity
3. Materials and Methods
3.1. Chemicals
3.2. Culture Conditions for A. platensis and Application of Drying Methods
3.3. Protein Determination
3.4. Phycobiliproteins
3.5. Chlorophyll and Carotenoids
3.6. Ascorbic and Dehydroascorbic Acid
3.7. Total Phenol and Total Flavonoid Analysis
3.8. Antioxidant Activity Assays
3.9. Phenolic Profile Identification
3.10. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Biomolecules | A. platensis Fresh | A. platensis Frozen | A. platensis Oven-Dried | A. platensis Freeze-Dried |
---|---|---|---|---|
C-Phycocyanin (mg g D.W.−1) | 44.81 ± 0.35b | 50.21 ± 0.74a | 18.88 ± 0.35d | 38.03 ± 0.50c |
Allophycocyanin (mg g D.W.−1) | 18.90 ± 1.03a | 4 ± 0.09b | 4.52 ± 0.51b | 5.48 ± 0.16b |
Phycoerythrin (mg g D.W.−1) | 5.28 ± 0.16a | 0.99 ± 0.25c | 1.47 ± 0.26c | 2.17 ± 0.11b |
Chlorophyll a (mg g D.W.−1) | 12.45 ± 0.04a | 9.95 ± 0.09b | 1.47 ± 0.03d | 6.44 ± 0.03c |
Carotenoids (mg g D.W.−1) | 3.82 ± 0.15a | 3.31 ± 0.05b | 0.90 ± 0.001d | 2.22 ± 0.004c |
Biomolecules | A. platensis Fresh | A. platensis Frozen | A. platensis Oven-Dried | A. platensis Freeze-Dried |
---|---|---|---|---|
Total Proteins (mg g D.W. −1) | 188.60 ± 13.55b | 283.96 ± 11.79a | 122.73 ± 2.53c | 167.09 ± 4.35b |
Ascorbic acid (mg g D.W.−1) | 1678.292.62b | 3149.54 ± 7.99a | 354.79 ± 0.93d | 1403.9 ± 11.49c |
Dehydroascorbic acid (mg g D.W.−1) | 1998.99 ± 7.01c | 1362.93 ± 22.78d | 3296.69 ± 15.56b | 4660.74 ± 34.56a |
Total Phenols (mg g D.W.−1) | 15.77 ± 1.10b | 22.65 ± 0.46a | 12.14 ± 1.84c | 11.91 ± 0.28c |
Total Flavonoids (mg g D.W.−1) | 20.82 ± 0.08b | 8.04 ± 0.29c | 4.31 ± 0.11d | 30.92 0.17a |
Compounds | Rt | A. platensis Fresh | A. platensis Oven-Dried | A. platensis Frozen | A. platensis Freeze-Dried |
---|---|---|---|---|---|
Gallic acid | 6.11 | + | + | + | + |
Catechin | 11.28 | + | + | + | - |
Caffeic acid | 13.22 | + | - | + | - |
p-Hydroxybenzoic acid | 14.13 | + | + | + | - |
p-Cumaric acid | 18.69 | + | + | - | + |
Ferulic acid | 18.81 | + | + | - | + |
Quercetin | 29.59 | + | - | + | + |
Genistein | 34.95 | + | + | + | + |
Kaempferol | 36.67 | + | + | + | + |
Biomass | C-PC | CAROT | CHL | PHEN | FLAVON | ASC | |
---|---|---|---|---|---|---|---|
DPPH | Fresh | ||||||
r | −0.994 | 0.879 | −0.736 | 0.546 | 0.147 | −0.087 | |
R2 | 0.989 | 0.77 | 0.536 | n.s. | n.s. | n.s. | |
Frozen | |||||||
r | −0.099 | −0.13 | −0.805 | −1.00 | −0.528 | −0.926 | |
R2 | n.s. | n.s. | 0.648 | 0.999 | n.s. | 0.857 | |
Oven-dried | |||||||
r | 0.624 | 0.776 | −0.928 | −0.586 | −0.863 | 0.541 | |
R2 | n.s. | 0.603 | 0.861 | n.s. | 0.745 | n.s. | |
Freeze-dried | |||||||
r | 0.961 | 0.926 | 0.895 | 0.676 | 0.977 | 0.709 | |
R2 | 0.924 | 0.858 | 0.801 | n.s. | 0.954 | 0.502 | |
TAC | Fresh | ||||||
r | 0.207 | 0.385 | −0.604 | 0.778 | 0.969 | −0.982 | |
R2 | n.s. | n.s. | n.s. | 0.606 | 0.939 | 0.965 | |
Frozen | |||||||
r | 0.084 | 0.116 | 0.796 | 1.00 | 0.516 | 0.920 | |
R2 | n.s. | n.s. | 0.634 | 1.00 | n.s. | 0.847 | |
Oven-dried | |||||||
r | 0.571 | −0.999 | 0.516 | 0.956 | 0.993 | 0.938 | |
R2 | n.s. | 0.999 | n.s. | 0.913 | 0.986 | 0.88 | |
Freeze-dried | |||||||
r | −0.934 | 0.899 | 0.863 | 0.627 | 0.960 | 0.66 | |
R2 | 0.872 | 0.808 | 0.745 | n.s. | 0.922 | n.s. |
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Papalia, T.; Sidari, R.; Panuccio, M.R. Impact of Different Storage Methods on Bioactive Compounds in Arthrospira platensis Biomass. Molecules 2019, 24, 2810. https://doi.org/10.3390/molecules24152810
Papalia T, Sidari R, Panuccio MR. Impact of Different Storage Methods on Bioactive Compounds in Arthrospira platensis Biomass. Molecules. 2019; 24(15):2810. https://doi.org/10.3390/molecules24152810
Chicago/Turabian StylePapalia, Teresa, Rossana Sidari, and Maria Rosaria Panuccio. 2019. "Impact of Different Storage Methods on Bioactive Compounds in Arthrospira platensis Biomass" Molecules 24, no. 15: 2810. https://doi.org/10.3390/molecules24152810
APA StylePapalia, T., Sidari, R., & Panuccio, M. R. (2019). Impact of Different Storage Methods on Bioactive Compounds in Arthrospira platensis Biomass. Molecules, 24(15), 2810. https://doi.org/10.3390/molecules24152810