FT-IR/ATR Solid Film Formation: Qualitative and Quantitative Analysis of a Piperacillin-Tazobactam Formulation
Abstract
:1. Introduction
2. Results
2.1. Identification of PIP and TAZ
2.1.1. Powder Spectra
2.1.2. Film Spectra
2.2. Quantification of PIP and TAZ
3. Discussion
4. Materials and Methods
4.1. Preparation of Standard Mixtures
4.2. Preparation of Solutions of Standard Mixtures
4.3. Development of Film Forming Methodology
4.4. FT-IR/ATR Spectra Acquisition
4.5. Spectra Processing
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Appendix A
References
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API | Wavenumber (cm−1) | Vibration Mode |
---|---|---|
PIP | 728, 762, 850, 890 | Aromatic CH wagging |
1072, 1094 | In-plane movement of aromatic carbons | |
1461, 1602 | Semi-circle stretching and Quadrum stretching of aromatic ring | |
1713, 1764 | Carbonyl group (CO) stretching | |
TAZ | 1135, 1191, 1312 | In-phase and Out-of-phase stretching of C-SO2-C |
1232, 1452 | N=N stretching | |
1385, 1616 | Carbonyl group (CO) stretching |
Wavenumber (cm−1) | Observation |
---|---|
873 | Intensity enhancement and change in intensity ratio against the characteristic PIP peak at 890 cm−1 |
945 | Intensity enhancement and change in intensity ratio between neighboring peaks at 933 and 949 cm−1 (area A) |
1023 | Intensity enhancement and change in intensity ratio against the neighboring peak at 1007 cm−1 |
1081 | Intensity enhancement and change in intensity ratio between neighboring peaks at 1072 and 1094 cm−1 (area B) |
1135 | Detection of TAZ shoulder |
% Mass Ratio TAZ-PIP | I(873)/I(890) | RSD% |
---|---|---|
50:50 | 2.925 | 3.14 |
45:55 | 2.493 | 2.19 |
25:75 | 1.138 | 3.48 |
15:85 | 0.699 | 2.22 |
10:90 | 0.517 | 3.89 |
5:95 | 0.367 | 1.90 |
Sample Availability: Samples of the compounds PIP and TAZ are available from the authors. |
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Chrisikou, I.; Orkoula, M.; Kontoyannis, C. FT-IR/ATR Solid Film Formation: Qualitative and Quantitative Analysis of a Piperacillin-Tazobactam Formulation. Molecules 2020, 25, 6051. https://doi.org/10.3390/molecules25246051
Chrisikou I, Orkoula M, Kontoyannis C. FT-IR/ATR Solid Film Formation: Qualitative and Quantitative Analysis of a Piperacillin-Tazobactam Formulation. Molecules. 2020; 25(24):6051. https://doi.org/10.3390/molecules25246051
Chicago/Turabian StyleChrisikou, Ioanna, Malvina Orkoula, and Christos Kontoyannis. 2020. "FT-IR/ATR Solid Film Formation: Qualitative and Quantitative Analysis of a Piperacillin-Tazobactam Formulation" Molecules 25, no. 24: 6051. https://doi.org/10.3390/molecules25246051