Quantitative Proteomics Explore the Potential Targets and Action Mechanisms of Hydroxychloroquine
Abstract
:1. Introduction
2. Results
2.1. Overview of the Quantitative Proteomics and Thermal Proteome Profiling Studies
2.2. Dynamics of the Proteome Induced by HCQ
2.3. Cellular Pathway Analysis of the Dynamic Proteins Induced by HCQ
2.4. Thermal Proteome Profiling of MIA PaCa-2 Cells Treated with HCQ
2.5. Identification of the Potential Binding Proteins of HCQ
3. Discussion
4. Materials and Methods
4.1. Cell Lines, Antibody, and TMT Agent
4.2. Cell Culture
4.3. Thermal Proteome Profiling
4.4. TMT Labeling
4.5. Proteomics Sample Analysis
4.6. LC-MS/MS Analysis
4.7. Data Analysis
4.8. Bioinformatic Analysis
4.9. Cell Viability Assay
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Gene | △Tm (°C) | Function |
---|---|---|
NQO2 | 12 | A quinone reductase |
GSR | 5.8 | Maintains high levels of reduced glutathione |
NAMPT | 2.1 | Catalyzes the condensation of nicotinamide |
KIF11 | 2.38 | Motor protein required for establishing a bipolar spindle during mitosis |
ALDH9A1 | 1.6 | Converts gamma-trimethylaminobutyraldehyde into gamma-butyrobetaine |
SEC23A | 1.4 | Component of the coat protein complex II (COPII) |
PUF60 | 1.3 | DNA- and RNA-binding protein |
PCM1 | 4.7 | Required for centrosome assembly and function |
NONO | 1.89 | DNA- and RNA binding protein |
ARFGAP1 | 1.98 | GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1) |
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Zhao, J.; Zhao, Z.; Hou, W.; Jiang, Y.; Liu, G.; Ren, X.; Liu, K.; Liu, H.; Chen, K.; Huang, H. Quantitative Proteomics Explore the Potential Targets and Action Mechanisms of Hydroxychloroquine. Molecules 2022, 27, 5175. https://doi.org/10.3390/molecules27165175
Zhao J, Zhao Z, Hou W, Jiang Y, Liu G, Ren X, Liu K, Liu H, Chen K, Huang H. Quantitative Proteomics Explore the Potential Targets and Action Mechanisms of Hydroxychloroquine. Molecules. 2022; 27(16):5175. https://doi.org/10.3390/molecules27165175
Chicago/Turabian StyleZhao, Jingxiang, Zhiqiang Zhao, Wanting Hou, Yue Jiang, Guobin Liu, Xuelian Ren, Kun Liu, Hong Liu, Kaixian Chen, and He Huang. 2022. "Quantitative Proteomics Explore the Potential Targets and Action Mechanisms of Hydroxychloroquine" Molecules 27, no. 16: 5175. https://doi.org/10.3390/molecules27165175