Next Article in Journal
Magnetic Torus Microreactor as a Novel Device for Sample Treatment via Solid-Phase Microextraction Coupled to Graphite Furnace Atomic Absorption Spectroscopy: A Route for Arsenic Pre-Concentration
Previous Article in Journal
Optimized Solid-Phase Mesh-Enhanced Sorption from Headspace (SPMESH) for Rapid Sub-ng/kg Measurements of 3-Isobutyl-2-methoxypyrazine (IBMP) in Grapes
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 27
Issue 19
10.3390/molecules27196214
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

The First 5′-Phosphorylated 1,2,3-Triazolyl Nucleoside Analogues with Uracil and Quinazoline-2,4-Dione Moieties: A Synthesis and Antiviral Evaluation

by
Dmitry A. Tatarinov
1,*,
Bulat F. Garifullin
1,
Mayya G. Belenok
1,
Olga V. Andreeva
1,
Irina Yu Strobykina
1,
Anna V. Shepelina
2,
Vladimir V. Zarubaev
3,
Alexander V. Slita
3,
Alexandrina S. Volobueva
3,
Liliya F. Saifina
1,
Marina M. Shulaeva
1,
Vyacheslav E. Semenov
1 and
Vladimir E. Kataev
1
1
Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center, Russian Academy of Sciences, Arbuzov Str., 8, 420088 Kazan, Russia
2
Department of Organic and Medicine Chemistry, Kazan Federal University, Kremlevskaya 18, 420008 Kazan, Russia
3
Pasteur Institute of Epidemiology and Microbiology, Mira Str., 14, 197101 Saint Petersburg, Russia
*
Author to whom correspondence should be addressed.
Molecules 2022, 27(19), 6214; https://doi.org/10.3390/molecules27196214
Submission received: 21 August 2022 / Revised: 12 September 2022 / Accepted: 16 September 2022 / Published: 21 September 2022
(This article belongs to the Section Medicinal Chemistry)
Browse Figures
Versions Notes

Abstract

:
A series of 5′-phosphorylated (dialkyl phosphates, diaryl phosphates, phosphoramidates, H-phosphonates, phosphates) 1,2,3-triazolyl nucleoside analogues in which the 1,2,3-triazole-4-yl-β-D-ribofuranose fragment is attached via a methylene group or a butylene chain to the N-1 atom of the heterocycle moiety (uracil or quinazoline-2,4-dione) was synthesized. All compounds were evaluated for antiviral activity against influenza virus A/PR/8/34/(H1N1). Antiviral assays revealed three compounds, 13b, 14b, and 17a, which showed moderate activity against influenza virus A (H1N1) with IC50 values of 17.9 μM, 51 μM, and 25 μM, respectively. In the first two compounds, the quinazoline-2,4-dione moiety is attached via a methylene or a butylene linker, respectively, to the 1,2,3-triazole-4-yl-β-D-ribofuranosyl fragment possessing a 5′-diphenyl phosphate substituent. In compound 17a, the uracil moiety is attached via the methylene unit to the 1,2,3-triazole-4-yl-β-D-ribofuranosyl fragment possessing a 5′-(phenyl methoxy-L-alaninyl)phosphate substituent. The remaining compounds appeared to be inactive against influenza virus A/PR/8/34/(H1N1). The results of molecular docking simulations indirectly confirmed the literature data that the inhibition of viral replication is carried out not by nucleoside analogues themselves, but by their 5′-triphosphate derivatives.

1. Introduction

The seven decades that have passed since the discovery of the structure of DNA have shown that analogues of natural nucleosides are the leading line of drugs used to fight infections caused by HIV, hepatitis B and C viruses, Dengue virus, Ebola virus, herpes virus, and others [1,2,3]. Moreover, it was representatives of nucleoside analogues that proved effective in COVID-19 chemotherapy [4]. The most well-known cyclic nucleoside analogues used in the treatment of various viral infections are shown in Figure 1. Chronologically, the first nucleoside analogue whose antiviral activity was detected was Idoxuridine (IdU), which was approved by the U.S. Food and Drug Administration (FDA) in 1963 for the treatment of herpes simplex virus (HSV) and varicella-zoster virus (VZV) [1] (Figure 1). Trifluoruridine (TFT) was also licensed for the treatment of herpes in 1980 [1]. Brivudine (BVDU) was approved by the FDA in 1980 against VZV [5]. Six years later, Vidarabine (AraA) was licensed for the treatment of HSV and VZV [1]. Ribavirin (RBV) is the first nucleoside analogue that has shown activity against several RNA viruses (hepatitis C, influenza A viruses, respiratory syncytial virus, etc.) [6]. In addition, Ribavirin is the only nucleoside analogue among all drugs approved by the FDA for the treatment of influenza [6]. Zidovudine (ZDV) (Figure 1) was not only the first drug approved for the treatment of HIV infection, but also the first nucleoside analogue for which a target was established—HIV reverse transcriptase (RNA-dependent DNA polymerase) [6]. Accordingly, Zidovudine was the first to be named a nucleoside reverse transcriptase inhibitor (NRTI). Didanosine (ddI) (Figure 1), synthesized in 1964 and approved by the FDA in 1991, appeared to be the second NRTI after Zidovudine for HIV treatment [2,6]. Zalcitabine (ddC) (Figure 1), synthesized in the late 1960s and released to the pharmaceutical market in 1992, became the third nucleoside analogue approved by the FDA for the treatment of HIV infection [2,6]. Stavudine (d4T) (Figure 1) was synthesized in the late 1960s, but its ability to inhibit HIV replication was discovered only in 1982 during the AIDS epidemic that began in New York City. Approved for use in 1994, Stavudine became the fourth drug among nucleoside analogues for the treatment of HIV infection [2,6]. Completing the list of NRTIs, Lamivudine (3TS), Abacavir (ABC), and Emtricitabine (FTC) were approved by the FDA for the treatment of HIV infection in 1996, 1998, and 2004, respectively [1,2,6] (Figure 1). Entecavir (ETV) and Telbivudine (LDT) (Figure 1) both were approved for the treatment of HBV infection in 2005 and 2006, respectively [3].
From 1986 to the 1990s, a series of studies showed that Zidovudine (ZDV) and Stavudine (d4T) in uninfected and HIV-infected cells were metabolized under the action of various cellular kinases (phosphotransferases) to their 5′-mono-, 5′-di-, and 5′-triphosphate derivatives [7]. It was later assumed that all nucleoside analogues are subjected to such metabolism and 5′-triphosphorylated derivatives formed in this way are their active forms. These 5′-triphosphate forms compete with natural nucleotides for binding to the active centers of viral RNA-dependent RNA polymerases (RdRp) and inhibit the synthesis of viral nucleic acids, which leads to blocking the replication of a virus [1,8,9].
Numerous studies have revealed the following features of the intracellular metabolism of nucleoside analogues affecting their antiviral activity. (i) Since nucleoside analogues (NA) are structurally different from natural nucleosides, their phosphorylation by nucleoside/nucleotide cellular kinases to generate the active NA-5′-triphosphate metabolites is often of limited efficiency [10,11,12]. Thus, for example, phosphorylation of Zidovudine monophosphate (ZDV-MP) to Zidovudine diphosphate (ZDV-DP) is very inefficient in lymphocytes and peripheral blood mononuclear (PBM) cells, and ZDV accumulates as a monophosphate representing approximately 95% of all its phosphorylated forms [10,12]. Consistent with the rate-limited formation of Stavudine monophosphate (d4T-MP) caused by poor recognition of d4T by cellular kinases, the majority of intracellular d4T remains in its unphosphorylated form [12]. Since Zidovudine and Stavudine are ineffectively phosphorylated by cellular kinases in resting PBM cells in vitro, they exhibit weak anti-HIV activity in them. In contrast, ddI, ddC, and 3TC (Figure 1) are phosphorylated quite efficiently in these types of cells in vitro and exhibit more potent anti-HIV activity [11]. (ii) The intracellular concentration of nucleoside analogue 5′-triphosphates is regulated by positive or negative feedback mechanisms on one or more enzymes in the phosphorylation pathway. Regulation of the pathway can be very complex and can be affected by other nucleosides (endogenous as well as exogenous) in multiple negative and positive feedback regulatory loops [12].
To overcome the problems of intracellular phosphorylation of nucleoside analogues caused by their poor recognition by cellular kinases (phosphotransferases), which leads to insignificant concentrations of active 5′-triphosphate forms and, consequently, to low antiviral activity, chemists focused on the synthesis of 5′-phosphorylated derivatives of nucleoside analogues. It was assumed that the introduction of synthesized 5′-mono-, 5′-di-, or 5′-triphosphate nucleoside analogues into an infected cell would avoid three, two, or even one stage of intracellular phosphorylation and deliver to a viral target already prepared active forms. The published various synthetic approaches for preparing 5′-mono-, 5′-di-, and 5′-triphosphate nucleoside analogues have been systematized and outlined in several reviews and books (see, for example, [13,14]).
Note that in parallel, in principle different directions of synthesis of antiviral nucleoside analogues began to develop. The thing is, the data accumulated in the 1950s that the nucleotides of naturally-occurring pyrimidines and purines are poorly incorporated into cell nucleic acids caused serious doubts about their ability to penetrate cells through lipid-rich cell membranes due to negative charges in phosphate groups at physiological pH. A. Montgomery was the first to suggest in 1961 that this difficulty could be overcome if it were possible to prepare a nucleotide ester that could penetrate through the cell wall and then be metabolized to the nucleotide itself [15]. This idea was soon developed, and it was proposed to use so-called prodrugs representing 5′-monophosphates of nucleoside analogues in which the monophosphate group is masked by any easily hydrolysable groups, thereby making the molecule more lipophilic and hence improving its transport into cells. Upon cell entry, the masking groups are enzymatically cleaved off to release free 5′-monophosphate, which is further transformed by cellular kinases to the active 5′-triphosphate form of the nucleoside analogue [16].
Several prodrug approaches have been developed to date. The best known among them are the bis-POM, bis-SATE, CycloSal, ProTide, and TriPPPro approaches. Chronologically, the first was the bis-POM approach, which uses bis-pivaloyloxymethyl (POM)-masking groups and was reported in 1983 by D. Farquhar [17] (Figure 2). This approach utilizes a carboxyesterase-catalyzed cleavage of the pivaloyl ester within the POM-masking group to yield the highly-reactive hydroxymethyl phosphotriester, which is converted into a mono(POM) phosphodiester, and, in turn, is hydrolyzed by a phosphodiesterase that affords the monophosphate of a parent nucleoside analogue [17]. Unfortunately, this approach cannot be considered successful because the delivery of one molecule of a parent nucleoside analogue in the form of a bis-POM prodrug results in the liberation of two equivalents of potentially toxic formaldehyde and pivalic acid. The following prodrug approach was reported by J. L. Imbach, who suggested to use bis(S-acyl-2-thioethyl (bis-SATE) nucleotides as prodrugs [18] (Figure 2). The bis-SATE approach is also not optimal, since as a result of the cleavage of bis-SATE-masking groups, two equivalents of episulfide are chronically and acutely toxic in mice and rats [19], and are released besides the monophosphate of a parent nucleoside analogue [18].
In 1996, C. Meier designed a new and entirely different prodrug approach, named the CycloSal approach, which uses salicyl alcohol as a cyclic bifunctional masking unit. Salicyl alcohol is attached to a phosphorus atom via a phenyl- and a benzyl ester bond, while a parent nucleoside analogue is attached through an alkyl ester bond (Figure 2) [20]. The introduction of these three different ester bonds allows sufficient discrimination in the process of enzymatic hydrolysis occurring by tandem (or cascade) mechanism. First, the phenolate is preferentially displaced at the phosphate group, and then spontaneous hydrolysis of the benzyl ester bond occurs, affording the 5′-monophosphate of the parent nucleoside analogue and salicylic alcohol, which is non-toxic [20]. The literature is full of examples of successful use of the CycloSal approach. Thus, the functionalization of 2′,3′-dideoxyadenosine with the 5′-cyclosaligenyl moiety increased its anti-HIV activity by 100 times [21,22]. The 5′-CycloSal derivative (prodrug) of 2′,3′-didehydro-2′,3′-dideoxyadenosine exceeded the anti-HIV activity of the parent nucleoside 400-fold [22]. The effectiveness of Abacavir (Figure 1) after its transformation into a 5′-cyclosaligenyl derivative improved 4 times [23], whereas the functionalization of Carbovir (6′-deoxy-2′,3′-didehydro-2′,3′-dideoxyguanosine) with the 5′-cyclosaligenyl moiety increased its anti-HIV activity by 30 times [23]. The functionalization of Brivudine (Figure 1) with a 5′-cyclosaligenyl moiety led to the appearance of its prodrug form of pronounced activity against the Herpes virus [24]. At the same time, the antiviral activity of Stavudine (Figure 1) and its 5′-cyclosaligenyl derivative appeared to be almost the same [25].
In the early 1990s, C. McGuigan proposed his prodrug approach, which he later called the ProTide approach [26]. In this approach, the 5′-phosphate substituent of nucleoside (nucleotide) analogues is masked by an aryl group and an amino acid ester residue. Such a phosphoramidate, which became known as ProTide (PROdrug + nucleoTIDE) [27] (Figure 2), is able to enter the cell via facilitated passive diffusion or by means of nucleoside transporters and afford a monophosphate form of a parent nucleoside analogue after cleavage of the masking groups via sequence enzyme-mediated and spontaneous steps, including the hydrolysis of the amino acid ester by carboxyesterase or cathepsin A, displacement of the phosphate phenol by the carboxylate anion, chemical hydrolysis, and cleavage of the amino acid moiety by the histidine triad nucleotide-binding protein [28]. Numerous studies have shown a clear correlation between the nature of aromatic and amino acid moieties and antiviral potency. ProTides with electron acceptor groups in the para-position of the aromatic moiety were the most active, while phosphoramidate with electron-donating groups showed the worst activity. ProTides possessing a phenyl moiety occupied an intermediate position [27]. As for the nature of the amino acid ester residue, alanine phosphoramidates proved to be strikingly more effective than other amino acids, for example, compared to leucine by 10 times and glycine by more than 100 times [27,29]. The literature provides many examples of the effectiveness of using the ProTide approach. For example, the 5′-(phenyl methoxy-L-alaninyl)phosphate of Zidovudine (ProTide-ZDV, Figure 2) showed more than 100-times better activity against HIV than the parent nucleoside analogue, Zidovudine (Figure 1) [27]. The ProTides of Stavudine and Abacavir (ProTide-d4T and ProTide-ABC, Figure 2) were more than 70-times and 20-times more active against HIV than their parent nucleoside analogues Stavudine and Abacavir, respectively (Figure 1) [27]. For 30 years, the ProTide approach has been applied to a wide range of nucleoside analogues. Dozens of phosphoramidate prodrugs have been synthesized, which have shown powerful antiviral activity against various RNA viruses [30,31,32]. These studies have paved the way for the discovery of such bright ProTides as Sofosbuvir (phosphoramidate of 2′-deoxy-2′-α-fluoro-2′-β-methyluridine) and Remdesivir (phosphoramidate 1′-cyano-7,9-dideaza-4-azaadenosine). Sofosbuvir was approved by the FDA in December 2013 for the treatment of HCV [33]. Remdesivir, which exhibits a wide range of activities against RNA viruses [4,28], is currently the only FDA-approved antiviral drug for the treatment of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection [34].
The prodrugs mentioned above intracellularly release monophosphate forms that still need to undergo two additional phosphorylation steps into their corresponding triphosphates’ active metabolites. In 2015, C. Meier discovered the unique method of delivering bioactive triphosphate of nucleoside analogues in the cell, which is known as the TriPPPro approach [35]. This approach uses the functionalization of 5ʹ-triphosphate forms of nucleoside analogues by two masking alkoxycarbonyloxybenzyl groups (Figure 2). Although TriPPPro compounds are still charged at the phosphate groups, obviously the modification at the γ-phosphate groups by lipophilic bioreversible moieties gives the molecule sufficient lipophilicity to penetrate the cell membrane. Thus, unlike previous approaches, by using the TriPPPro approach, nucleoside analogues do not need any intracellular phosphorylation. The literature provides examples of the effectiveness of the TriPPPro approach. Both TriPPPro derivatives of Stavudine (TriPPPro-d4T, Figure 2) and TriPPPro derivatives of Abacavir (TriPPPro-ABC, Figure 2) are superior to their parent nucleoside analogues Stavudine (d4T, Figure 1) and Abacavir (ABC, Figure 1) in antiviral activity against HIV-1 and HIV-2—the first by 10 times [36] and the second by 3–6 times [37,38]. However, the antiviral activity of Zalcitabine (ddC, Figure 1) and its TriPPPro derivative against HIV-1 and HIV-2 are the same [37].
Surprisingly, despite many publications on the synthesis of 5′-phosphorylated derivatives of nucleoside analogues [13,14], we found in the literature just a few articles on the study of their antiviral activity. Firstly, in 1989, A. Kraevsky’s group synthesized a 5′-H-phosphonate derivative of Zidovudine, which demonstrated high anti-HIV activity [39]. In 1999, this prodrug form of Zidovudine was registered in the Russian Federation as an antiviral agent for combined antiretroviral therapy against HIV infection called Nicavir [39,40]. Secondly, in 2007, D. Liotta’s group reported that the 5′-triphosphate of 5-fluoro-1-[cis-3-(hydroxymethyl)-cyclobutyl]-cytosine showed in vitro anti-HIV activity against recombinant HIV reverse transcriptases (RT) and wild-type HIV RT (IC50 = 4.7 and 6.9 μM, respectively), whereas the parent nucleoside was inactive up to 100 μM. The same high activity was demonstrated by the 5′-triphosphate of Lamivudine (3TC, Figure 1) [41]. Third, four synthesized phosphoramidate prodrugs of β-D-2′-deoxy-2′-fluoro-2′-C-methyl-7-deazapurine nucleoside analogues showed no anti-HCV activity, whereas appropriate 5′-triphosphates of parent nucleosides demonstrated potent inhibitory effects against individual wild-type and S282T mutant HCV polymerases. Cellular pharmacology studies in Huh-7 cells revealed that 5′-triphosphates were not formed at significant levels from these phosphoramidate prodrugs, indicating that insufficient cellular phosphorylation was responsible for the lack of their anti-HCV activity [42]. Fourth, it was found that while 2′,3′-dideoxy-β-D-apio-D-furanonucleosides failed to show anti-HIV activity, their 5′-triphosphate prodrugs were readily accepted by a viral DNA polymerase to act as a DNA chain terminator [43]. Fifth, the ability of the 5′-triphosphates of four HIV RT inhibitors, Zidovudine, Lamivudine, Emtricitabine, and Abacavir (Figure 1), to be incorporated by the RNA-dependent RNA polymerase (RdRp) of SARS-CoV, wherein they also terminated further polymerase extension—thereby inhibiting virus replication—was demonstrated [44]. Of all the five cases listed above, only the groups of A. Kraevsky [39,40] and D. Liotta [41] incubated triphosphates of nucleoside analogues in infected cells (in other cases, experiments were conducted with individual viral RdRp) and found their antiviral activity. In our opinion, this is enough to cast doubt on the statement made in the 1950s that negatively-charged 5′-triphosphate nucleoside analogues cannot penetrate into cells through lipid-rich cell membranes [15]. Indeed, during 2000–2010, integral proteins were identified in human cells, of which not only nucleoside analogues, but also a variety of organic anions, penetrate cells [9,45]. The above leads to the idea of the expediency of continuing the synthesis and comparison of the antiviral activity of the three groups of compounds: nucleoside analogues, their 5′-phosphorylated derivatives, and their various prodrug forms. Moreover, it is interesting to consider not only the nucleoside analogues already used in clinical settings, but also other antiviral nucleoside analogues with an unusual structure.
Recently, we synthesized a series of 1,2,3-triazolyl nucleoside analogues in which a nucleic base (uracil or thymine) or its derivative (6-methyluracil or quinazoline-2,4-dione) was connected to the D-ribofuranose residue via a 1,2,3-triazolyl bridge and an alkyl (methylene, propylene or butylene) linker [46,47]. The evaluation of antiviral potency revealed compounds that showed moderate (IC50 = 30 and 42 μM) in vitro activity against influenza A (H1N1) virus [47]. Considering the literature data presented above, it was of interest to compare the antiviral activity of these 1,2,3-triazolyl nucleoside analogues [47] with the antiviral activity of their 5′-phosphorylated derivatives, which both possess a negatively-charged phosphate or H-phosphonate group and neutral ether or phosphoramidate group. In our opinion, this would be the first comparison of the antiviral activity of a nucleoside analogue, its negatively-charged 5′-monophosphate and 5′-H-phosphonate derivative, and its 5′-phosphorylated derivatives with a masked phosphate group (i.e., prodrug forms), which would allow, at least indirectly, the assessment of whether a synthetic nucleotide (i.e., prodrug forms) penetrates into the cell or not. Herein, we report on the synthesis and antiviral evaluation against influenza A (H1N1) virus of a series of 5′-phosphorylated (dialkyl phosphates, diaryl phosphates, phosphoramidates, H-phosphonates, phosphates) derivatives of 1,2,3-triazolyl nucleoside analogues in which the uracil or quinazoline-2,4-dione moiety is attached to the D-ribofuranose residue by means of a 1,2,3-triazolyl bridge and the methylene or butylene linker.

2. Results and Discussion

2.1. Chemistry

2.1.1. Synthesis of 1,2,3-Triazolyl Nucleoside Analogues

The synthesis of starting 1,2,3-triazolyl nucleoside analogues was carried out in three stages in accordance with the described methods [46,47]. During the first stage (Scheme 1), uracil 1a and quinazoline-2,4-dione 1b were converted into 2,4-bistrimethylsilyl esters 2a and 2b by reflux with an excess of hexamethyldisilazane in toluene in the presence of H2SO4. Then, these esters were selectively alkylated in situ with propargyl bromide and 6-iodo-1-hexine in DMF to afford 1-alkyne derivatives of uracil 3a and 4a and quinazoline-2,4-dione 3b and 4b at 40–61% yields.
During the second stage (Scheme 2), commercial D-ribose 1c was converted by interaction with MeOH into methyl-D-α/β-ribofuranoside, which was acylated in situ to obtain methyl-2,3,5-tri-O-acetyl-β-D-ribofuranoside 2c.
Then, by reaction of monosaccharide 2c with a mixture of glacial AcOH and Ac2O in the presence of H2SO4, tetra-acetylated β-D-ribofuranose 3c was obtained, the interaction of which with trimethylsilylazide (TMSN3) in the presence of tin tetrachloride led to 2,3,5-tri-O-acetyl-β-D-ribofuranosylazide 4c at a 95% yield (Scheme 2).
During the third stage (Scheme 3), 1-alkyne derivatives of uracil 3a and 4a and quinazoline-2,4-dione 3b and 4b were involved in the copper sulfate-catalyzed reaction of azide-alkyne cycloaddition (CuAAC) with 2,3,5-tri-O-acetyl-β-D-ribofuranosylazide 4c.
The reaction was carried out in a mixture of t-BuOH/H2O (1:1), using equimolecular amounts of azide and alkyne components, CuSO4·5H2O (10 mol%), and sodium ascorbate (20 mol%) according to the procedure previously described [46]. The 1,2,3-triazolyl nucleoside analogues with acylated hydroxyl groups 5a, 6a, 5b, and 6b were obtained at 76–89% yields (Scheme 3). The formation of a 1,2,3-triazole ring was confirmed by the appearance of a signal of the triazolyl proton, C-5′′-H, within the range of 7.50–7.97 ppm in the 1H NMR spectra of compounds 5a, 6a, 5b, and 6b. The triazolyl carbons, C-4″, in the 13C NMR spectra resonated within the range of 142.85–147.68 ppm, and signals of the triazolyl carbons, C-5″, were observed within the range of 120.10–123.54 ppm. All these facts fully corresponded to the characteristic features of 1,2,3-triazoles in 1H and 13C NMR spectra described in the literature [48,49,50,51]. Compounds 5a, 6a, 5b, and 6b were obtained as β-anomers. This was evidenced by the resonance of the anomeric protons of acetylated D-ribofuranose residues as doublets within the range of 5.98–6.13 ppm with vicinal coupling constants within the range of 3.5–4.2 Hz, which corresponded to the literature data [52].
For further phosphorylation of 1,2,3-triazolyl nucleoside analogues by very reactive phosphorus-containing reagents—for example, H-phosphonates—it was necessary to remove the protection of the hydroxyl group at the C-5′ atom, leaving the protection of the hydroxyl groups at the C-2′ and C-3′ atoms. For this purpose, first, the acetyl protection of the sugar residue of compounds 5a, 6a, 5b, and 6b was removed with a solution of sodium methylate in methanol, and then compounds 7a, 8a, 7b, and 8b were obtained at 89–91% yields and were converted to the 1,2,3-triazolyl nucleoside analogues 9a, 10a, 9b, and 10b with isopropylidene protection of hydroxyl groups at the C-2′ and C-3′ atoms and a free hydroxyl group at the C-5′ atom by interaction with 2,2-dimethoxypropane in acetone in the presence of para-toluene sulfonic acid (Scheme 3).

2.1.2. Synthesis of 5′-Phosphorylated Derivatives of 1,2,3-Triazolyl Nucleoside Analogues

The synthesis of 1,2,3-triazolyl nucleotide analogues with 5′-dialkyl- and 5′-diphenyl phosphate moieties at the C-5′ atom was carried out by analogy with the known method of phosphorylation of nucleoside analogues with D-arabinofuranose residue [53]. Compounds 7a, 8a, 7b, and 8b with unprotected hydroxyl groups were involved in a reaction with diethyl phosphorochloridate or diphenyl phosphorochloridate in pyridine at room temperature to afford 5′-diethyl phosphates 11a, 12a, 11b, and 12b, and 5′-diphenyl phosphates 13a, 14a, 13b, and 14b (Scheme 4). These compounds were isolated by flash chromatography on silica gel at 16–42% yields, respectively.
The appearance of a signal at −1.5 ppm in the 31P NMR spectra of compounds 11a,b and 12a,b and a signal of −12.1 ppm in the 31P NMR spectra of compounds 13a,b and 14a,b confirmed the addition of a diethyl phosphate or diphenyl phosphate group to the C-5′ atom of the initial nucleosides 7a,b and 8a,b. In general, a multiplicity, chemical shifts, and an integral intensity of signals in the 1H NMR spectra of synthesized diethyl phosphates 11a,b and 12a,b as well as diphenyl phosphates 13a,b and 14a,b are consistent with the structures shown in Scheme 4.
The synthesis of 1,2,3-triazolyl nucleoside analogues with a 5′-phosphoramidate moiety was carried out in three stages (Scheme 5) by analogy with the procedure developed in 1993 for the synthesis of a prodrug form of the antiretroviral drug Zidovudine [26]. According to the literature data [27,29], a phenyl group was selected as the aromatic moiety of the phosphoramidate fragment and an alanine residue was selected as the amino acid moiety of the phosphoramidate fragment. At the first stage, phenyl phosphorodichloridate 5c was prepared by the reaction of phenol with phosphorus oxychloride. During the second stage, condensation of phenyl phosphorodichloridate 5c with L-alanine methyl ether hydrochloride 6c in the presence of triethylamine at −78 °C gave phenyl methoxyalaninyl phosphorochloridate 7c a yield of more than 97%. During the third stage, amidophosphorochloridate 7c was involved in a reaction with 2′,3′-O-isopropylidene-protected nucleosides 9a,b and 10a,b in dichloromethane. A pyridine mixture in the presence of triethylamine to afford 5′-phosphoramidates 15a,b and 16a,b were isolated by flash chromatography as a mixture of two diastereomers at 31–67% yields. Then, their 2,3-O-isopropylidene protection was removed with a 50% aqueous solution of trifluoroacetic acid (TFA) to obtain the target phosphoramidates 17a,b and 18a,b at 33–64% yields (Scheme 5).
The synthesis of 1,2,3-triazolyl nucleoside analogues with a 5′-H-phosphonate moiety was performed similarly to the known procedure [54] by the interaction of 2′,3′-O-isopropylidene-protected nucleosides 9a,b and 10a,b with salicyl phosphorochloridate (2-chloro-1,3,2-benzodioxaphosphorin-4-one) 8c, which was prepared by the reaction of salicylic acid with phosphorus trichloride as previously described [55] (Scheme 6). The completeness of the reaction was monitored by 31P NMR spectroscopy. The resulting salicyl phosphites 19a,b and 20a,b were manifested in the 31P{1H} NMR spectra in the form of two singlets near 125 ppm due to the formation of diastereomers differing in the configuration of the asymmetric phosphorus atom. In the 31P NMR spectra of 19a,b and 20a,b, these signals transformed to triplets with spin-coupling constants 3JPH = 9.0 Hz. The resulting nucleoside 5′-salicyl phosphites 19a,b and 20a,b were hydrolyzed in situ to afford 2′,3′-O-isopropylidene-protected nucleoside 5′-H-phosphonate monoesters 21a,b and 22a,b, which were isolated by flash chromatography on silica gel at 31–64% yields (Scheme 6). The formation of the H-phosphonate group was confirmed by the 31P and 31P{1H} NMR spectra. The asymmetry of the phosphorus atom during the formation of the H-phosphonate anion disappeared, therefore the 31P {1H} NMR spectra of 21a,b and 22a,b showed a single singlet at 10 ppm and the 31P NMR spectra of 21a,b and 22a,b exhibited a doublet of triplets within the range of 1JPH = 617–622 Hz. Treatment of 2′,3′-O-isopropylidene-protected nucleoside H-phosphonate monoesters 21a,b and 22a,b with aqueous TFA gave the desired unprotected nucleoside 5′-H-phosphonate monoesters 23a,b and 24a,b in 50–80% yields.
The synthesis of 1,2,3-triazolyl nucleoside analogues with a 5′-phosphate moiety was initially carried out by analogy with the known methodology [56,57] by the interaction of nucleoside analogues 7a,b and 8a,b with phosphorus oxychloride in trimethyl phosphate, which was used as a solvent, followed by hydrolysis (Scheme 7). This methodology makes it possible to use nucleosides with unprotected hydroxyl groups of the ribofuranose residue as starting compounds, that is, it is characterized by the ease of implementation and, in addition, high conversion [56,57]. Indeed, according to TLC data, the interaction of 1,2,3-triazolyl nucleoside analogues 7a,b and 8a,b with phosphorus oxychloride proceeded with almost complete conversion of the initial nucleosides (>95%). The formation of target products 25a,b and 26a,b was confirmed by the 31P{1H} NMR spectra of the reaction mixtures, in which a singlet was present within the range of −0.5–3 ppm. Moreover, the 31P NMR spectra of the reaction mixtures exhibited a phosphorus atom of the C5′H2OP(O)O22− fragment as a triplet with a spin-coupling constant 3JPH = 6.7 Hz.
Unfortunately, the isolation of 5′-phosphates 25a,b and 26a,b by chromatography methods turned out to be difficult due to the impurity of trimethylammonium salts of phosphoric acid, which could not be separated either by flash chromatography with gradient elution or chromatography on ion exchange resins. Having failed in the isolation of individual 5′-phosphates 25a,b and 26a,b synthesized by the known methodology [56,57], we used another approach based on the oxidation of 5′-trimethylsilyl-H-phosphonate monoesters with elemental iodine [58]. The 2′,3-O-isopropylidene-protected nucleoside H-phosphonate monoesters 21a,b and 22a,b were first converted into the trimethylsilyl H-phosphonate diesters 27a,b and 28a,b (using 3 mol. equiv. of TMSCl in CH2Cl2), which were oxidized in situ by elemental iodine to afford the halogenophosphates 29a,b and 30a,b—which were hydrolyzed in situ with aqueous triethylamine to give 2,3-O-isopropylidene-protected nucleoside 5′-phosphates 31a,b and 32a,b, which were isolated by flash chromatography on silica gel in 40–55% yields (Scheme 7). The formation of compounds 31a,b and 32a,b was confirmed by the appearance of a single singlet at –0.5 ppm in their 31P{1H} NMR spectra, which underwent a significant broadening in their 31P NMR spectra. The absence of the 1JPH constant indicated complete conversion of the initial nucleoside H-phosphonate monoesters 21a,b and 22a,b. Then, the isopropylidene protection of 1,2,3-triazolyl nucleotide analogues 31a,b and 32a,b was removed with an aqueous solution of trifluoroacetic acid (TFA) to obtain target 5′-phosphates of 1,2,3-triazolyl nucleoside analogues 25a,b and 26a,b in 56–78% yields (Scheme 7).

2.2. Antiviral Evaluation

The in vitro antiviral activity of the synthesized compounds 11a,b–14a,b; 17a,b; 18a,b; and 23a,b–26a,b was evaluated regarding the A/Puerto Rico/8/34 (H1N1) strain of influenza virus. The resulting data expressed as virus-inhibiting activity (IC50), a medium cytotoxic concentration (CC50), and a selectivity index (SI), which is the CC50/IC50 ratio, are presented in Table 1.
In addition, the data on the antiviral activity of the previously studied parent 1,2,3-triazolyl nucleoside analogues 33a,b and 34a,b (Figure 3) are also given in the Table. That is, in Table 1, one can compare the antiviral activity of the parent 1,2,3-triazolyl nucleoside analogues 33a,b and 34a,b; their prodrug forms, namely diethyl phosphates 11a,b and 12a,b, diphenyl phosphates 13a,b and 14a,b, phosphoramidates 17a,b and 18a,b; and their H-phosphonate derivatives 23a,b and 24a,b, and monophosphate derivatives 25a,b and 26a,b.
Note that among the parent nucleoside analogues 33a,b and 34a,b (Figure 3), 1,2,3-triazolyl derivatives of uridine 33a and 34a were inactive. Moderate activity against H1N1 (A/PR/8/34) influenza virus was demonstrated by compounds 33b and 34b (IC50 values are 42 and 30 μM, respectively), in which the uracil moiety was replaced by the quinazoline-2,4-dione moiety. Among all synthesized 5′-phosphorylated derivatives (i.e., prodrug forms) of nucleoside analogue 33b, the only compound, namely diphenyl phosphate 13b, showed antiviral activity, which, by the way, exceeded the activity of the parent compound 33b by 2 times (IC50 = 17.9 vs. IC50 = 42 μM), and the selectivity index increased by 7 times (SI = 28 vs. SI = 3). Similarly, among all synthesized 5′-phosphorylated derivatives (i.e., prodrug forms) of the nucleoside analogue 34b (Figure 3), antiviral activity was demonstrated by only one compound, namely diphenyl phosphate 14b. Its activity approximately corresponded to the activity of the parent compound 34b (IC50 = 51 μM vs. IC50 = 30 μM), although its cytotoxicity increased 7-fold (CC50 = 98 μM vs. CC50 = 710 μM). Regarding 1,2,3-triazolyl derivative of uridine 34a, all its synthesized 5′-phosphorylated derivatives, namely prodrug forms 12a, 14a, and 18a, and charged derivatives 24a and 26a, were inactive against H1N1 (A/PR/8/34) influenza virus. At the same time, to our surprise, the 5′-phosphoramidate derivative 17a of the uridine analogue 33a appeared to be moderately active against H1N1 (A/PR/8/34) influenza virus (IC50 = 25 μM) with a good selectivity index of S = 21, although its parent compound 33a did not show antiviral activity against this virus.
By analyzing the results obtained from the point of view of the literature data presented in the Introduction section on the penetration of nucleoside analogues into the cell and their metabolism in the cell into active triphosphate forms [1,7,8,9], the following conclusions can be made. Firstly, the fact that all synthesized H-phosphonates 23a,b and 24a,b and phosphates 25a,b and 26a,b did no show antiviral activity is easily explained by the presence of negative charges preventing their penetration into the cell, and indicates that receptor proteins that are capable of transporting organic anions into the cell [9,45] were unsuitable for transporting H-phosphonates 23a,b and 24a,b and phosphates 25a,b and 26a,b into the cell. Secondly, according to the literature data discussed above [1,7,8,9], antiviral activity characterized by IC50 values of 42 μM and 30 μM was shown not by the parent nucleoside analogues 33b and 34b, but by their 5′-triphosphate derivatives 35b and 36b (Figure 3). That is, compounds 33b and 34b somehow (e.g., with the help of corresponding transport proteins) penetrated into the cell, wherein they were sequentially metabolized under the action of cellular kinases (phosphotransferases) first to their 5′-monophosphate derivatives 25b and 26b, then to their 5′-diphosphate derivatives, and finally, to 5′-triphosphate derivatives 35b and 36b (Figure 3), which somehow inhibited the replication of the virus [1,7,8,9]. Thirdly, according to the literature data [1,7,8,9], diphenyl phosphates 13b and 14b after penetration into the cell were enzymatically converted into monophosphates 25b and 26b, which were subsequently metabolized by cellular kinases into the corresponding active 5′-triphosphate forms 35b and 36b (Figure 3), which have shown antiviral activity with IC50 values of 17.9 and 51 μM. Therefore, it is not surprising that diphenyl phosphate 14b, which is the prodrug form of the nucleoside analogue 34b, showed approximately the same antiviral activity as the parent compound 34b (IC50 = 51 μM vs. IC50 = 30 μM). In both cases, according to the literature data [1,7,8,9], virus replication was inhibited by the 5′-triphosphate derivative 36b, which formed during intracellular metabolism (Figure 3).
It may seem strange that diphenyl phosphate 13b, being the prodrug form of the nucleoside analogue 33b, showed antiviral activity that was twice as high as the activity of the parent compound 33b (IC50 = 17.9 μM vs. IC50 = 42 μM), although according to the literature data [1,7,8,9], in both cases, 5′-triphosphate 36b was the active form. However, the literature has provided many examples of when the antiviral activity of a prodrug form—because of some unknown reason—significantly exceeded the antiviral activity of a parent nucleoside analogue [21,22,23,27,30,31,32], or even when a prodrug form showed antiviral activity that was absent from a parent nucleoside analogue [24]. Apparently, such a case can be observed for the 1,2,3-triazolyl analogue of uridine 33a and its prodrug form, phosphoramidate 17a. The 1,2,3-triazolyl analogue of uridine 33a was completely inactive against H1N1 (A/PR/8/34) influenza virus, while its prodrug form, phosphoramidate 17a, demonstrated moderate inhibitory activity against this virus (IC50 = 25 μM) and a good selectivity index (SI = 21). Taking into account the literature data on the metabolism of nucleoside analogues and their prodrug forms inside the cell [1,7,8,9], which were discussed in the Introduction section, we can assume the following reasons for the absence of antiviral activity for the 1,2,3-triazolyl analogue of uridine 33a and moderate antiviral activity for its prodrug form, phosphoramidate 17a: (i) the nucleoside analogue 33a for some reason could not penetrate into the cell; (ii) the nucleoside analogue 33a penetrated into the cell but was not recognized by cellular kinases, that is, the process of its phosphorylation to the active 5′-triphosphate form 35a (Figure 3) did not start; (iii) phosphoramidate 17a, in its differences from diethyl phosphate 11a and diphenyl phosphate 13a, somehow penetrated into the cell; and (iv) phosphoramidate 17a was enzymatically hydrolyzed into monophosphate 25a, which was then, according to the literature data [16], converted by cellular kinases into triphosphate 35a, and it was triphosphate 35a that showed antiviral activity against H1N1 (A/PR/8/34) influenza virus with the IC50 value of 25 μM and the SI value of 21 (Table 1).
As for the 1,2,3-triazolyl analogue of uridine 34a, neither it nor its prodrug forms, namely diethyl phosphate 12a, diphenyl phosphate 14a, and phosphoramidate 18a, have demonstrated any antiviral activity against H1N1 (A/PR/8/34) influenza virus. The reason can be assumed to be one single but significant difference in the structure of these compounds from the structure of the 1,2,3-triazolyl analogue of uridine 33a and its prodrug forms 11a, 13a, and 17a. This difference is the remoteness of the uracil moiety from the 1,2,3-triazolylribofuranosyl fragment by a considerable distance (butylene linker instead of methylene).

2.3. Molecular Docking

Continuing the interpretation of the data obtained on the antiviral activity of the synthesized compounds (Table 1) from the point of view of the literature data, wherein despite whatever nucleoside analogues or their prodrug forms are introduced into the cell, it is their 5′-triphosphate derivatives that inhibit viral replication [1,7,8,9], we carried out molecular docking of triphosphates 35a,b and 36a,b (Figure 3). We chose the N-terminal endonuclease domain of the polymerase acidic polypeptide (PA-Nter) of the RNA-dependent RNA polymerase (RdRp) of influenza virus A (H1N1) as a drug target [59]. The PA-Nter domain has a cation-dependent endonuclease active-site core that is responsible for viral RNA replication (PDB code 4AWK [59]). A distinctive feature of this active site is that its structure and topology are approximately the same for all RNA viruses [60,61], including all influenza A subtypes and strains [62,63]. In addition, this site is not very selective to the structure of substrates [60,61]. All of the above makes the PA-Nter domain of RdRp an attractive drug target. Thus, we have evaluated the binding of triphosphates 35a,b and 36a,b to the PA-Nter endonuclease domain (PDB code 4AWK [59]). The positions of the optimized docking models of the compounds demonstrating the best binding energy in the PA-Nter active site are shown in Figure 4.
According to the molecular docking calculation, the compounds 35b, 36b, and 36a are approximately located in one region of space and their triphosphate fragments are localized deep in the cavity of the P-Nter active site. On the contrary, compound 35a is localized in such a way that its triphosphate fragment is located at the mouth of this cavity. This arrangement of the studied molecules seems surprising because compound 36a, which possesses the uracil moiety, is not located in the same region of the active site in which compound 35a, which also possesses the uracil moiety, but rather is located in the region in which compounds 35b and 36b, which possess the quinazoline-2,4-dione moiety, are located.
The reason can be assumed to be a significant structural difference between compounds 35a and 36a. If in the first compound only the methylene unit separates the uracil moiety from the 1,2,3-triazolylribofuranosyl fragment, then in the second one the uracil moiety is kept away from the 1,2,3-triazolylribofuranose fragment by the length of the butylene chain and the significantly increased volume of compound 36a, which does not allow it to localize in the same area of space as compound 35a. However, once in the same region of space as compounds 35b and 36b, compound 36a did not find amino acid residues with which it could form hydrogen bonds.
As a result, compound 36a is retained in the active site only due to van der Waals interactions and shows the lowest binding energy among the studied compounds (7.8 kcal/mol, Table 2). The compounds 35b and 36b are retained in the cavity of the active site by hydrogen bonding of their triphosphate fragments with the amino acid residue Val122 and the π-π stacking interaction of their quinazoline-2,4-dione moiety with the amino acid residue, Tyr24.
The hydrogen bonding of the triphosphate fragment of compound 35b with the Lys134 amino acid residue provides a stronger retention of 35b in the cavity of the P-Nter active site compared to 36b. Accordingly, the binding energy of compound 35b (9.8 kcal/mol) is higher than the binding energy of compound 36b (8.9 kcal/mol, Table 2). The compound 35a, being localized in another region of the cavity of the active P-Nter site, is well retained in it due to the hydrogen bonding of its triphosphate fragment with the amino acid residues, Arg84 and Trp88, as evidenced by the binding energy of 9.1 kcal/mol (Table 2).
Thus, according to the molecular docking calculation, triphosphate 35b has the highest binding energy to the PA-Nter active site among the compounds studied (9.8 kcal/mol). The binding energies of 35a and 36b are worse (9.1 and 8.9 kcal/mol, respectively). Triphosphate 36a has the lowest binding energy (7.8 kcal/mol). It can be seen that the trend of antiviral activity of prodrug forms 13b, 14b, and 17a of the parent 1,2,3-triazolyl nucleoside analogues 33a,b and 34a,b (Figure 3) correlates with the trend of the binding energy of 5′-triphosphate derivatives 35a,b and 36a,b of the parent 1,2,3-triazolyl nucleoside analogues 33a,b and 34a,b with the active site of RdRp. Indeed, the decrease in antiviral activity during the transition from the lead compound diphenyl phosphate 13b with maximum antiviral activity in the studied series of compounds (IC50 = 17.9 μM) to phosphoramidate 17a with moderate antiviral activity (IC50 = 25 μM) correlates with the decrease of binding energy during the transition from the corresponding 5′-triphosphate 35b (9.1 kcal/mol) to 5′-triphosphate 35a (8.2 kcal/mol). Similarly, the transition from diphenyl phosphate 14b, which showed moderate antiviral activity (IC50 = 51 μM), to the completely inactive parent 1,2,3-triazolyl nucleoside analog 34a and its inactive prodrug forms 12a, 14a, and 18a (IC50 > 180 μM) correlates with a significant decrease in the binding energy during the transition from the corresponding 5′-triphosphate 36b (8.4 kcal/mol) to 5′-triphosphate 36a (7.8 kcal/mol). Thus, in our opinion, the results of the molecular docking simulation indirectly confirm the literature data [1,7,8,9], which suggests that it is the 5′-triphosphate derivatives of nucleoside analogues that inhibit viral replication (See Supplementary Materials).

3. Conclusions

In summary, a series of prodrug forms of previously-studied [47] parent 1,2,3-triazolyl nucleoside analogues 33a,b and 34a,b (Figure 3) were synthesized. These prodrug forms are 5′-diethyl phosphates 11a,b and 12a,b; 5′-diphenyl phosphates 13a,b and 14a,b; and 5′-phosphoramidates 17a,b and 18a,b. In addition, 5′-H-phosphonate derivatives 23a,b and 24a,b and 5′-monophosphate derivatives 25a,b and 26a,b of parent nucleoside analogues 33a,b and 34a,b were synthesized. In all these compounds, the 5′-phosphorylated 1,2,3-triazole-4-yl-β-D-ribofuranose fragment is attached via the methylene unit or butylene chain to the N-1 atom of the heterocycle moiety (uracil or quinazoline-2,4-dione). Antiviral assays against H1N1 (A/PR/8/34) influenza virus revealed three prodrugs, namely 5′-diphenylphosphates 13b and 14b and 5′-phosphoramidates 17a, which showed moderate activity against this virus with IC50 values of 17.9 µM, 51 µM, and 25 µM, respectively. Prodrug forms 13b and 17a showed good selectivity indices (28 and 21, respectively), while prodrug form 14b demonstrated a low selectivity index (SI = 2) due to its high toxicity (CC50 = 98 µM). All other compounds appeared to be inactive against H1N1 (A/PR/8/34) influenza virus. Taking into account the literature data that virus replication is inhibited not by the nucleoside analogues themselves, but by their 5′-triphosphate derivatives [1,7,8,9], we carried out molecular docking simulations of 5′-triphosphate derivatives 35a,b and 36a,b of the parent nucleoside analogues 33a,b and 34a,b (Figure 3). The N-terminal endonuclease domain of the polymerase acidic polypeptide (PA-Nter) of the RNA-dependent RNA polymerase (RdRp) of the H1N1 (A/PR/8/34) influenza virus was used as a drug target. It was found that the antiviral activity of prodrug forms 13b, 14b, and 17a in terms of IC50 values correlates with the binding energies of the corresponding triphosphates 35b, 36b, and 35a. Thus, the maximum antiviral activity among the number of studied compounds (IC50 = 17.9 μM) shown by diphenyl phosphate 13b, which possesses the quinazoline-2,4-dione moiety as a nucleic base, corresponds to the maximum binding energy (9.8 kcal/mol, Table 2) of the corresponding triphosphate 35b with amino acid residues of the active site PA-Nter of the RdRp of H1N1 (A/PR/8/34) influenza virus.
The absence of antiviral activity against influenza A (H1N1) virus (IC50 > 180 μM) of the parent nucleoside analogue 34a with the uracil moiety as a nucleic base and its prodrug forms 12a, 14a, and 18a correlates with the minimum binding energy (7.8 kcal/mol) of the corresponding triphosphate 36a (Table 2). Thus, the results obtained indirectly confirm the literature data that the inhibition of viral replication is carried out not by nucleoside analogues themselves, but by their 5-triphosphate derivatives [1,7,8,9].

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/molecules27196214/s1, general information (S2), procedures for the synthesis of the compounds (S3, S5, S10–S11, S17–S18, S23), characterization of the compounds synthesized (S3–S5, S5–S10, S11–S17, S18–S22, S23–S28) including their NMR spectra (Figures S1–S101), description of the antiviral assays (S130) and the molecular docking study (S130). References [46,64,65,66,67,68,69,70] are cited in the Supplementary data.

Author Contributions

Supervision, methodology, analyses of obtained results, writing the manuscript, V.E.K.; synthesis, analyses of obtained results, O.V.A.; synthesis, molecular docking, B.F.G.; synthesis D.A.T., M.G.B., I.Y.S., A.V.S. (Anna V. Shepelina), L.F.S., M.M.S. and V.E.S.; antiviral assays, V.V.Z., A.S.V. and A.V.S. (Alexander V. Slita). All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the Ministry of Science and Higher Education of the Russian Federation, Agreement number 075-15-2020-777.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The data presented in this study are available on request from V.E.K.

Acknowledgments

The authors from the Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center, RAS are grateful to the Assigned Spectral-Analytical Center of FRC Kazan Scientific Center of RAS for technical assistance during research.

Conflicts of Interest

The authors declare no conflict of interest.

Sample Availability

Samples of the compounds are not available.

References

  1. Pastuch-Gawołek, G.; Gillner, D.; Krol, E.; Walczak, K.; Wandzik, I. Selected nucleos(t)ide-based prescribed drugs and their multi-target activity. Eur. J. Pharm. 2019, 865, 172747. [Google Scholar] [CrossRef] [PubMed]
  2. Seley-Radtke, K.L.; Yates, M.K. The evolution of nucleoside analogue antivirals: A review for chemists and non-chemists. Part 1: Early structural modifications to the nucleoside scaffold. Antivir. Res. 2018, 154, 66–86. [Google Scholar] [CrossRef] [PubMed]
  3. Slusarczyk, M.; Serpi, M.; Pertusati, F. Phosphoramidates and phosphonamidates (ProTides) with antiviral activity. Antivir. Chem. Chemother. 2018, 26, 2040206618775243. [Google Scholar] [CrossRef] [PubMed]
  4. Pruijssers, A.J.; Denison, M.R. Nucleoside analogues for the treatment of coronavirus infections. Curr. Opin. Virol. 2019, 35, 57–62. [Google Scholar] [CrossRef]
  5. De Clercq, E.; Descamps, J.; De Somer, P.; Barr, P.J.; Jones, A.S.; Walker, R.T. (E)-5-(2-Bromovinyl)-2’-deoxyuridine: A potent and selective anti-herpes agent. Proc. Natl. Acad. Sci. USA 1979, 76, 2947–2951. [Google Scholar] [CrossRef]
  6. De Clercq, E.; Li, G. Approved Antiviral Drugs over the Past 50 Years. Clin. Microbiol. Rev. 2016, 29, 695–747. [Google Scholar] [CrossRef]
  7. Balzarini, J. Metabolism and mechanism of antiretroviral action of purine and pyrimidine derivatives. Pharm. World Sci. 1993, 16, 113–126. [Google Scholar] [CrossRef]
  8. Eyer, L.; Nencka, R.; De Clercq, E.; Seley-Radtke, K.; Ruzek, D. Nucleoside analogs as a rich source of antiviral agents active against arthropod-borne flaviviruses. Antivir. Chem. Chemother. 2018, 26, 2040206618761299. [Google Scholar] [CrossRef]
  9. Jordheim, L.P.; Durantel, D.; Zoulim, F.; Dumontet, C. Advances in the development of nucleoside and nucleotide analogues for cancer and viral diseases. Nat. Rev. Drug Discov. 2013, 12, 447–464. [Google Scholar] [CrossRef]
  10. Martin, J.C.; Hitchcock, M.J.M.; Kaul, S.; Dunkle, L.M.; Sterzycki, R.Z.; Mansuri, M.M.; Fridland, A.; Ghazzouli, I.; Kaul, S.; Dunkle, L.M.; et al. Comparative studies of 2′,3′-didehydro-2′3′-dideoxythymidine (d4T) with other pyrimidine nucleoside analogues. Ann. N. Y. Acad. Sci. 1990, 616, 22–28. [Google Scholar] [CrossRef]
  11. Gao, W.-Y.; Agbaria, R.; Driscoll, J.S.; Mitsuya, H. Divergent anti-human immunodeficiency virus activity and anabolic phosphorylation of 2′,3′-dideoxynucleoside analogs in resting and activated human cells. J. Biol. Chem. 1994, 269, 12633–12638. [Google Scholar] [CrossRef]
  12. Stein, D.S.; Moore, K.H. Phosphorylation of nucleoside analog antiretrovirals: A review for clinicians. Pharmacotherapy 2001, 21, 11–34. [Google Scholar] [CrossRef] [PubMed]
  13. Johnson, D.C., II; Widlanski, T.S. Overview of the Synthesis of Nucleoside Phosphates and Polyphosphates. In Current Protocols in Nucleic Acid Chemistry; John Wiley & Sons: New York, NY, USA, 2003; Volume 15, pp. 1–31. [Google Scholar]
  14. Roy, R.; Depaix, A.; Périgaud, C.; Peyrottes, S. Recent trends in nucleotide synthesis. Chem. Rev. 2016, 116, 7854–7897. [Google Scholar] [CrossRef] [PubMed]
  15. Montgomery, J.A.; Thomas, H.J.; Schaeffer, H.J. Synthesis of potential anticancer agents. XXVIII. Simple esters of 6-mercaptopurine ribonucleotide. J. Org. Chem. 1961, 26, 1929–1933. [Google Scholar] [CrossRef]
  16. Stella, V.J. Prodrugs and site-specific drug delivery. J. Med. Chem. 1980, 23, 1275–1285. [Google Scholar] [CrossRef]
  17. Farquhar, D.; Srivastva, D.N.; Kuttesch, N.J.; Saunders, P.P. Biologically reversible phosphate-protective group. J. Pharm. Sci. 1983, 72, 324–325. [Google Scholar] [CrossRef]
  18. Valette, G.; Pompon, A.; Girardet, J.-L.; Cappellacci, L.; Franchetti, P.; Grifantini, M.; La Colla, P.; Loi, A.G.; Perigaud, C.; Gosselin, G.; et al. Decomposition pathways and in vitro HIV inhibitory effects of isoddA pronucleotides: Toward a rational approach for intracellular delivery of nucleoside 5′-monophosphates. J. Med. Chem. 1996, 39, 1981–1990. [Google Scholar] [CrossRef]
  19. Meier, C. Pro-nucleotides—Recent advances in the design of efficient tools for the delivery of biologically active nucleoside monophosphates. Synlett 1998, 3, 233–242. [Google Scholar] [CrossRef]
  20. Meier, C. 2-Nucleos-5′-yl-4H-1,3,2-benzodioxaphosphinin-2-oxides—A new concept for lipophilic, potential prodrugs of biologically active nucleoside monophosphates. Angew. Chem. Int. Ed. Engl. 1996, 35, 70–72. [Google Scholar] [CrossRef]
  21. Meier, C.; Knispel, T.; Marquez, V.E.; Siddiqui, M.A.; De Clercq, E.; Balzarini, J. cycloSal-pronucleotides of 2′-Fluoro-ara- and 2′-Fluoro-ribo-2′,3′-dideoxyadenosine as a strategy to bypass a metabolic blockade. J. Med. Chem. 1999, 42, 1615–1624. [Google Scholar] [CrossRef]
  22. Meier, C.; Knispel, T.; De Clercq, E.; Balzarini, J. cycloSal-pronucleotides of 2′,3′-dideoxyadenosine and 2′,3′-dideoxy-2′,3′-didehydroadenosine: Synthesis and antiviral evaluation of a highly efficient nucleotide delivery system. J. Med. Chem. 1999, 42, 1604–1614. [Google Scholar] [CrossRef] [PubMed]
  23. Balzarini, J.; Haller-Meier, F.; De Clercq, E.; Meier, C. Antiviral activity of cyclosaligenyl prodrugs of acyclovir, carbovir and abacavir. Antivir. Chem. Chemother. 2002, 12, 301–306. [Google Scholar] [CrossRef] [PubMed]
  24. Meier, C. cycloSal-pronucleotides—Design of chemical trojan horses. Mini Rev. Med. Chem. 2002, 2, 219–234. [Google Scholar] [CrossRef] [PubMed]
  25. Meier, C.; Balzarini, J. Application of the cycloSal-prodrug approach for improving the biological potential of phosphorylated biomolecules. Antivir. Res. 2006, 71, 282–292. [Google Scholar] [CrossRef]
  26. Cahard, D.; McGuigan, C.; Balzarini, J. Aryloxy phosphoramidate triesters as Pro-Tides. Mini Rev. Med. Chem. 2004, 4, 371–381. [Google Scholar] [CrossRef]
  27. Mehellou, Y.; Balzarini, J.; McGuigan, C. Aryloxy phosphoramidate triesters: A technology for delivering monophosphorylated nucleosides and sugars into cells. ChemMedChem 2009, 4, 1779–1791. [Google Scholar] [CrossRef]
  28. Yan, L.; Cao, R.; Zhang, H.; Li, Y.; Li, W.; Li, X.; Fan, S.; Li, S.; Zhong, W. Design, synthesis and evaluation of 2′-acetylene-7-deaza-adenosine phosphoamidate derivatives as anti-EV71 and anti-EV-D68 agents. Eur. J. Med. Chem. 2021, 226, 113852. [Google Scholar] [CrossRef]
  29. McGuigan, C.; Pathirana, R.N.; Mahmood, N.; Hay, A.J. Aryl phosphate derivatives of AZT inhibit HIV replication in cells where the nucleoside is poorly active. Bioorg. Med. Chem. Lett. 1992, 2, 701–704. [Google Scholar] [CrossRef]
  30. McGuigan, C.; Madela, K.; Aljarah, M.; Gilles, A.; Brancale, A.; Zonta, N.; Chamberlain, S.; Vernachio, J.; Hutchins, J.; Hall, A.; et al. Design, synthesis and evaluation of a novel double pro-drug: INX-08189. A new clinical candidate for hepatitis C virus. Bioorg. Med. Chem. Lett. 2010, 20, 4850–4854. [Google Scholar] [CrossRef]
  31. Singh, U.S.; Mulamoottil, V.A.; Chu, C.K. 2′-Fluoro-6′-methylene carbocyclic adenosine and its phosphoramidate prodrug: A novel anti-HBV agent, active against drug-resistant HBVmutants. Med. Res. Rev. 2018, 38, 977–1002. [Google Scholar] [CrossRef]
  32. Wang, G.; Lim, S.P.; Chen, Y.-L.; Hunziker, J.; Rao, R.; Gu, F.; She, C.C.; Ghafar, N.A.; Xu, H.; Chan, K.; et al. Structure-activity relationship of uridine-based nucleoside phosphoramidate prodrugs for inhibition of dengue virus RNA-dependent RNA polymerase. Bioorg. Med. Chem. Lett. 2018, 28, 2324–2327. [Google Scholar] [CrossRef] [PubMed]
  33. Mehellou, Y.; Rattan, H.S.; Balzarini, J. The ProTide prodrug technology: From the concept to the clinic. J. Med. Chem. 2018, 61, 2211–2226. [Google Scholar] [CrossRef] [PubMed]
  34. Schooley, R.T.; Carlin, A.F.; Beadle, J.R.; Valiaeva, N.; Zhang, X.-Q.; Clark, A.E.; McMillan, R.E.; Leibel, S.L.; McVicar, R.N.; Xie, J.; et al. Rethinking Remdesivir: Synthesis, antiviral activity, and pharmacokinetics of oral lipid prodrugs. Antimicrob. Agents Chemother. 2021, 65, e01155-21. [Google Scholar] [CrossRef] [PubMed]
  35. Gollnest, T.; de Oliveira, T.D.; Schols, D.; Balzarini, J.; Meier, C. Lipophilic prodrugs of nucleoside triphosphates as biochemical probes and potential antivirals. Nat. Comm. 2015, 6, 8716–8730. [Google Scholar] [CrossRef] [PubMed]
  36. Jia, X.; Schols, D.; Meier, C. Anti-HIV-active nucleoside triphosphate prodrugs. J. Med. Chem. 2020, 63, 6003–6027. [Google Scholar] [CrossRef] [PubMed]
  37. Gollnest, T.; de Oliveira, T.D.; Rath, A.; Hauber, I.; Schols, D.; Balzarini, J.; Meier, C. Membrane-permeable triphosphate prodrugs of nucleoside analogues. Angew. Chem. 2016, 55, 5255–5258. [Google Scholar] [CrossRef]
  38. Weising, S.; Sterrenberg, V.; Schols, D.; Meier, C. Synthesis and antiviral evaluation of TriPPPro-AbacavirTP, TriPPPro-CarbovirTP and their 1′,2′-cis-disubstituted analogues. ChemMedChem 2018, 13, 1771–1778. [Google Scholar] [CrossRef]
  39. Khandazhinskaya, A.; Matyugina, E.; Shirokova, E. Anti-HIV therapy with AZT prodrugs: AZT phosphonate derivatives, current state and prospects. Expert Opin. Drug Metabol. Toxicol. 2010, 6, 701–714. [Google Scholar] [CrossRef]
  40. Malin, A.A.; Ostrovskii, V.A. Synthesis of thymidine derivatives as potential pharmaceuticals against HIV/AIDS infection. Russ. J. Org. Chem. 2001, 37, 759–780. [Google Scholar] [CrossRef]
  41. Li, Y.; Mao, S.; Hager, M.W.; Becnel, K.D.; Schinazi, R.F.; Liotta, D.C. Synthesis and evaluation of 2ʹ-substituted cyclobutyl nucleosides and nucleotides as potential anti-HIV agents. Bioorg. Med. Chem. Lett. 2007, 17, 3398–3401. [Google Scholar] [CrossRef] [Green Version]
  42. Shi, J.; Zhou, L.; Zhang, H.; McBrayer, T.R.; Detorio, M.A.; Johns, M.; Bassit, L.; Powdrill, M.H.; Whitaker, T.; Coats, S.J.; et al. Synthesis and antiviral activity of 2′-deoxy-2′-fluoro-2′-C-methyl-7-deazapurine nucleosides, their phosphoramidate prodrugs and 5′-triphosphates. Bioorg. Med. Chem. Lett. 2011, 21, 7094–7098. [Google Scholar] [CrossRef] [PubMed]
  43. Toti, K.S.; Derudas, M.; Pertusati, F.; Sinnaeve, D.; Van den Broeck, F.; Margamuljana, L.; Martins, J.C.; Herdewijn, P.; Balzarini, J.; McGuigan, C.; et al. Synthesis of an apionucleoside family and discovery of a prodrug with anti-HIV activity. J. Org. Chem. 2014, 79, 5097–5112. [Google Scholar] [CrossRef]
  44. Chien, M.; Anderson, T.K.; Jockusch, D.; Tao, C.; Li, X.; Kumar, S.; Russo, J.J.; Kirchdoerfer, R.N.; Ju, J. Nucleotide Analogues as Inhibitors of SARS-CoV-2 Polymerase, a Key Drug Target for COVID-19. J. Proteome Res. 2020, 19, 4690–4697. [Google Scholar] [CrossRef] [PubMed]
  45. Cano-Soldado, P.; Pastor-Anglada, M. Transporters that translocate nucleosides and structural similar drugs: Structural requirements for substrate recognition. Med. Res. Rev. 2012, 32, 428–457. [Google Scholar] [CrossRef] [PubMed]
  46. Andreeva, O.V.; Belenok, M.G.; Saifina, L.F.; Shulaeva, M.M.; Dobrynin, A.B.; Sharipova, R.R.; Voloshina, A.D.; Saifina, A.F.; Gubaidullin, A.T.; Khairutdinov, B.I.; et al. Synthesis of novel 1,2,3-triazolyl nucleoside analogues bearing uracil, 6-methyluracil, 3,6-dimethyluracil, thymine, and quinazoline-2,4-dione moieties. Tetrahedron Lett. 2019, 60, 151276. [Google Scholar] [CrossRef]
  47. Andreeva, O.V.; Garifullin, B.F.; Zarubaev, V.V.; Slita, A.V.; Yesaulkova, I.L.; ·Saifina, L.F.; Shulaeva, M.M.; Belenok, M.G.; Semenov, V.E.; Kataev, V.E. Synthesis of 1,2,3-triazolyl nucleoside analogues and their antiviral activity. Mol. Divers. 2020, 25, 473–490. [Google Scholar] [CrossRef]
  48. Fan, W.-Q.; Katritzky, A.R. Comprehensive Heterocyclic Chemistry; Katritzky, A.R., Rees, C.W., Scriven, E.F.V., Eds.; Pergamon: Oxford, UK, 1997; Volume 4, pp. 11–14. [Google Scholar]
  49. Chatzileontiadou, D.S.M.; Parmenopoulou, V.; Manta, S.; Kantsadi, A.L.; Kylindri, P.; Griniezaki, M.; Kontopoulou, F.; Telopoulou, A.; Prokova, H.; Panagopoulos, D.; et al. Triazole double-headed ribonucleosides as inhibitors of eosinophil derived neurotoxin. Bioorg. Chem. 2015, 63, 152–165. [Google Scholar] [CrossRef]
  50. Alexandrova, L.A.; Efremenkova, O.V.; Andronova, V.L.; Galegov, G.A.; Solyev, P.N.; Karpenko, I.L.; Kochetkov, S.N. 5-(4-Alkyl-1,2,3-Triazol-1-yl)methyl Derivatives of 2′-Deoxyuridine as Inhibitors of Viral and Bacterial Growth. Russ. J. Bioorg. Chem. 2016, 42, 677–684. [Google Scholar] [CrossRef]
  51. Chatzileontiadou, D.S.M.; Tsika, A.C.; Diamantopoulou, Z.; Delbé, J.; Badet, J.; Courty, J.; Skamnaki, V.T.; Parmenopoulou, V.; Komiotis, D.; Hayes, J.M.; et al. Evidence for Novel Action at the Cell-Binding Site of Human Angiogenin Revealed by Heteronuclear NMR Spectroscopy, in silico and in vivo Studies. ChemMedChem 2018, 13, 259–269. [Google Scholar] [CrossRef]
  52. Nisic, F.; Speciale, G.; Bernardi, A. Stereoselective Synthesis of α- and β-Glycofuranosyl Amides by Traceless Ligation of Glycofuranosyl Azides. Chem. Eur. J. 2012, 18, 6895–6906. [Google Scholar] [CrossRef]
  53. Colin, B.; Jones, N.M.; McGuigan, C.; Riley, P.A. Synthesis and biological evaluation of some phosphate triester derivatives of the anti-cancer drug AraC. Nucl. Acids Res. 1989, 17, 7195–7201. [Google Scholar] [CrossRef] [PubMed]
  54. Sun, Q.; Edathil, J.P.; Wu, R.; Smidansky, E.D.; Cameron, C.E.; Peterson, B.R. One-Pot Synthesis of Nucleoside 5′-Triphosphates from Nucleoside 5′-H-Phosphonates. Org. Lett. 2008, 10, 1703–1706. [Google Scholar] [CrossRef] [PubMed]
  55. Young, R.W. A Re-examination of the Reaction Between Phosphorus Trichloride and Salicylic Acid. J. Am. Chem. Soc. 1952, 74, 1672–1673. [Google Scholar] [CrossRef]
  56. Yoshikawa, M.; Kato, T.; Takenishi, T. A Novel Method for Phosphorylation of Nucleosides to 5′-Nucleotides. Tetrahedron Lett. 1967, 8, 5065–5068. [Google Scholar] [CrossRef]
  57. Ikemoto, T.; Haze, A.; Hatano, H.; Kitamoto, Y.; Ishida, M.; Nara, K. Phosphorylation of Nucleosides with Phosphorus Oxychloride in Trialkyl Phosphate. Chem. Pharm. Bull. 1995, 43, 210–215. [Google Scholar] [CrossRef]
  58. Rachwalak, M.; Rozniewska, M.; Golebiewska, J.; Jakubowski, T.; Sobkowski, M.; Romanowska, J. A practical synthesis of nucleoside 5′-diphosphates from nucleoside 5′-H-phosphonate monoesters. Synth. Commun. 2020, 50, 3836–3844. [Google Scholar] [CrossRef]
  59. Kowalinski, E.; Zubieta, C.; Wolkerstorfer, A.; Szolar, O.H.J.; Ruigrok, R.W.H.; Cusack, S. Structural Analysis of Specific Metal Chelating Inhibitor Binding to the Endonuclease Domain of Influenza pH1N1 (2009) Polymerase. PLoS Pathog. 2012, 8, e1002831. [Google Scholar] [CrossRef]
  60. Jockusch, S.; Tao, C.; Li, X.; Anderson, T.K.; Chien, M.; Kumar, S.; Russo, J.J.; Kirchdoerfer, R.N.; Ju, J. A Library of Nucleotide Analogues Terminate RNA Synthesis Catalyzed by Polymerases of Coronaviruses that Cause SARS and COVID-19. Antivir. Res. 2020, 180, 104857. [Google Scholar] [CrossRef]
  61. Jácome, R.; Campillo-Balderas, J.A.; Ponce de León, S.; Becerra, A.; Lazcano, A. Sofosbuvir as a potential alternative to treat the SARS-CoV-2 epidemic. Sci. Rep. 2020, 10, 9294. [Google Scholar] [CrossRef]
  62. Das, K.; Aramini, J.M.; Ma, L.-C.; Krug, R.M.; Arnold, E. Structures of influenza A proteins and insights into antiviral drug targets. Nat. SMB 2010, 17, 530–538. [Google Scholar] [CrossRef] [Green Version]
  63. Fudo, S.; Yamamoto, N.; Nukaga, M.; Odagiri, T.; Tashiro, M.; Hoshino, T. Two distinctive binding modes of endonuclease inhibitors to the N-terminal region of influenza virus polymerase acidic subunit. Biochemistry 2016, 55, 2646–2660. [Google Scholar] [CrossRef] [PubMed]
  64. Singh, U.S.; Chung, K.; Chu, C.K. Synthesis of 2′-deoxy-2′-fluoro-2′-C-methyl spiro cyclopentyl carbocyclic uridine analog as potential inhibitors of HCV NS5B polymerase. Nucl. Nucl. Nucl. Acids 2020, 30, 52–68. [Google Scholar] [CrossRef] [PubMed]
  65. Koizumi, T.; Arai, Y.; Yoshii, E. The Reaction of o-Phenylenediamine with Phenyl Phosphorodichloridate. Synthesis and Reactions of 2-Phenoxy-1,3-Dihydro-2-H-1,3,2-Benzodiazaphosphole-2-Oxide and Related Compounds. Chem. Pharm. Bull. 1973, 21, 202–206. [Google Scholar] [CrossRef]
  66. McGuigan, C.; Pathirana, R.N.; Balzarini, J.; De Clercq, E. Intracellular Delivery of Bioactive AZT Nucleotides by Aryl Phosphate Derivatives of AZT. J. Med. Chem. 1993, 36, 1048–1052. [Google Scholar] [CrossRef] [PubMed]
  67. Trott, O.; Olson, A.J. AutoDock Vina: Improving the speed and accuracy of docking with a new scoring function, efficient optimization and multithreading. J. Comput. Chem. 2010, 31, 455–461. [Google Scholar] [CrossRef] [PubMed]
  68. Berman, H.M.; Westbrook, J.; Feng, Z.; Gilliland, G.; Bhat, T.N.; Weissig, H.; Shindyalov, I.N.; Bourne, P.E. The protein data bank. Nucleic Acids Research 2000, 28, 235–242. [Google Scholar] [CrossRef] [PubMed]
  69. HyperChem Professional 8.0 (2007). Hypercube, Inc. Available online: http://www.hyper.com/?tabid=360. (accessed on 26 July 2022).
  70. O’Boyle, N.M.; Banck, M.; James, C.A.; Morley, C.; Vandermeersch, T.; Hutchison, G.R. Open babel: An open chemical toolbox. J. Cheminform 2011, 3, 33. [Google Scholar] [CrossRef] [Green Version]
Molecules 27 06214 g001 550
Figure 1. The most well-known antiviral cyclic nucleoside analogues.
Figure 1. The most well-known antiviral cyclic nucleoside analogues.
Molecules 27 06214 g001
Molecules 27 06214 g002 550
Figure 2. Schematic representation of the most known prodrugs.
Figure 2. Schematic representation of the most known prodrugs.
Molecules 27 06214 g002
Molecules 27 06214 sch001 550
Scheme 1. Synthesis of pyrimidine derivatives containing a ω-alkyne substituent at the N-1 atom.
Scheme 1. Synthesis of pyrimidine derivatives containing a ω-alkyne substituent at the N-1 atom.
Molecules 27 06214 sch001
Molecules 27 06214 sch002 550
Scheme 2. Synthesis of 2,3,5-tri-O-acetyl-β-D-ribofuranosyl azide 4c.
Scheme 2. Synthesis of 2,3,5-tri-O-acetyl-β-D-ribofuranosyl azide 4c.
Molecules 27 06214 sch002
Molecules 27 06214 sch003 550
Scheme 3. Synthesis of 1,2,3-triazolyl nucleoside analogues.
Scheme 3. Synthesis of 1,2,3-triazolyl nucleoside analogues.
Molecules 27 06214 sch003
Molecules 27 06214 sch004 550
Scheme 4. Synthesis of 5′-diethyl- and 5′-diphenylphosphates of 1,2,3-triazolyl nucleoside analogues.
Scheme 4. Synthesis of 5′-diethyl- and 5′-diphenylphosphates of 1,2,3-triazolyl nucleoside analogues.
Molecules 27 06214 sch004
Molecules 27 06214 sch005 550
Scheme 5. Synthesis of 5′-phosphoramidates of 1,2,3-triazolyl nucleoside analogues.
Scheme 5. Synthesis of 5′-phosphoramidates of 1,2,3-triazolyl nucleoside analogues.
Molecules 27 06214 sch005
Molecules 27 06214 sch006 550
Scheme 6. Synthesis of 5′-H-phosphonates of 1,2,3-triazolyl nucleoside analogues.
Scheme 6. Synthesis of 5′-H-phosphonates of 1,2,3-triazolyl nucleoside analogues.
Molecules 27 06214 sch006
Molecules 27 06214 sch007a 550Molecules 27 06214 sch007b 550
Scheme 7. Synthesis of 5′- phosphates of 1,2,3-triazolyl nucleoside analogues.
Scheme 7. Synthesis of 5′- phosphates of 1,2,3-triazolyl nucleoside analogues.
Molecules 27 06214 sch007aMolecules 27 06214 sch007b
Molecules 27 06214 g003 550
Figure 3. Parent 1,2,3-triazolyl nucleoside analogues 33a,b and 34a,b and their 5′-triphosphate derivatives 35a,b and 36a,b.
Figure 3. Parent 1,2,3-triazolyl nucleoside analogues 33a,b and 34a,b and their 5′-triphosphate derivatives 35a,b and 36a,b.
Molecules 27 06214 g003
Molecules 27 06214 g004 550
Figure 4. Molecular docking simulations of the optimized docking model of compounds 35a,b and 36a,b in the PA-Nter (PDB code 4AWK) active site of the RdRp of influenza virus A (H1N1) were obtained in the lowest-energy conformations.
Figure 4. Molecular docking simulations of the optimized docking model of compounds 35a,b and 36a,b in the PA-Nter (PDB code 4AWK) active site of the RdRp of influenza virus A (H1N1) were obtained in the lowest-energy conformations.
Molecules 27 06214 g004
Table
Table 1. Antiviral activity against A/Puerto Rico/8/34 (H1N1) influenza virus and cytotoxicity of 5′-phosphorylated 1,2,3-triazolyl nucleoside analogues 11a,b–14a,b; 17a,b; 18a,b; 23a,b–26a,b; and their parent compounds 33a,b and 34a,b.
Table 1. Antiviral activity against A/Puerto Rico/8/34 (H1N1) influenza virus and cytotoxicity of 5′-phosphorylated 1,2,3-triazolyl nucleoside analogues 11a,b–14a,b; 17a,b; 18a,b; 23a,b–26a,b; and their parent compounds 33a,b and 34a,b.
CompoundCC50 a (μM)IC50 b (μM)SI cCompoundCC50 a (μM)IC50 b (μM)SI c
Molecules 27 06214 i001
11a		>650434 ± 392Molecules 27 06214 i002
12a		>596250 ± 222
Molecules 27 06214 i003
13a		>538121 ± 74Molecules 27 06214 i004
14a		>500183 ± 153
Molecules 27 06214 i005
17a		>53025 ± 421Molecules 27 06214 i006
18a		>463>4631
Molecules 27 06214 i007
23a		>612204 ± 283Molecules 27 06214 i008
24a		>564218 ± 323
Molecules 27 06214 i009
25a		>494>4941Molecules 27 06214 i010
26a		>463195 ± 262
Molecules 27 06214 i011
11b		>587411 ± 511Molecules 27 06214 i012
12b		>542>5421
Molecules 27 06214 i013
13b		>49417.9 ± 328Molecules 27 06214 i014
14b		98 ± 651 ± 42
Molecules 27 06214 i015
17b		>487>4871Molecules 27 06214 i016
18b		>456>4561
Molecules 27 06214 i017
23b		>555176 ± 213Molecules 27 06214 i018
24b		>516>5161
Molecules 27 06214 i019
25b		>458159 ± 193Molecules 27 06214 i020
26b		>430>4301
Molecules 27 06214 i021
33a		>880 d>880 d1Molecules 27 06214 i022
34a		>817 d343 ± 412
Molecules 27 06214 i023
33b		132 ± 9 d42 ± 5 d3Molecules 27 06214 i024
34b		>719 d30 ± 4 d24
Molecules 27 06214 i025
Rimantadine340 ± 1677 ± 84
Molecules 27 06214 i026
Ribavirin		94 ± 4831 ± 93
Molecules 27 06214 i027
Oseltamivir carboxylate		>2000.3 ± 0.06>667
a CC50 is the median cytotoxic concentration, i.e., the concentration causing 50% cell death. b IC50 is the concentration causing 50% inhibition of virus replication. c SI is the selectivity index, which is the CC50/IC50 ratio. d Data from [47].
Table
Table 2. Binding energies and ligand–protein interactions of 5′-triphosphates 35a,b and 36a,b obtained by molecular docking simulations.
Table 2. Binding energies and ligand–protein interactions of 5′-triphosphates 35a,b and 36a,b obtained by molecular docking simulations.
Compound−Ebind (kcal/mol)Ligand-Protein Interactions
Molecules 27 06214 i028
35a		9.1H bonding: Arg84, Trp88
Molecules 27 06214 i029
35b		9.8H bonding: Val122, Lys134
π-π: Tyr24
Molecules 27 06214 i030
36a		7.8no H bonding
Molecules 27 06214 i031
36b		8.9H bonding: Glu80, Val122
π-π: Tyr24
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Tatarinov, D.A.; Garifullin, B.F.; Belenok, M.G.; Andreeva, O.V.; Strobykina, I.Y.; Shepelina, A.V.; Zarubaev, V.V.; Slita, A.V.; Volobueva, A.S.; Saifina, L.F.; et al. The First 5′-Phosphorylated 1,2,3-Triazolyl Nucleoside Analogues with Uracil and Quinazoline-2,4-Dione Moieties: A Synthesis and Antiviral Evaluation. Molecules 2022, 27, 6214. https://doi.org/10.3390/molecules27196214

AMA Style

Tatarinov DA, Garifullin BF, Belenok MG, Andreeva OV, Strobykina IY, Shepelina AV, Zarubaev VV, Slita AV, Volobueva AS, Saifina LF, et al. The First 5′-Phosphorylated 1,2,3-Triazolyl Nucleoside Analogues with Uracil and Quinazoline-2,4-Dione Moieties: A Synthesis and Antiviral Evaluation. Molecules. 2022; 27(19):6214. https://doi.org/10.3390/molecules27196214

Chicago/Turabian Style

Tatarinov, Dmitry A., Bulat F. Garifullin, Mayya G. Belenok, Olga V. Andreeva, Irina Yu Strobykina, Anna V. Shepelina, Vladimir V. Zarubaev, Alexander V. Slita, Alexandrina S. Volobueva, Liliya F. Saifina, and et al. 2022. "The First 5′-Phosphorylated 1,2,3-Triazolyl Nucleoside Analogues with Uracil and Quinazoline-2,4-Dione Moieties: A Synthesis and Antiviral Evaluation" Molecules 27, no. 19: 6214. https://doi.org/10.3390/molecules27196214

Article Metrics

Back to TopTop