Relative Stabilities of Conserved and Non-Conserved Structures in the OB-Fold Superfamily
Abstract
:1. Introduction
2. Results
2.1. Length distribution of OB-fold secondary structures
2.2. Contact densities distinguish conserved from non-conserved structures
2.3. Sequence hydrophobicity
2.4. Theoretical B-factors
2.5. Experimental B-factors
2.6. Differences between conserved and non-conserved structure from NMR data
3. Discussion
4. Conclusions
5. Experimental Section
5.1. OB-fold database selection
5.2. Secondary structure assignment
5.3. Analysis of sequence and structure parameters
Acknowledgments
References and Notes
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2° Structure | # Residues b | # Contacts (raw) | # Contacts (normalized) | Theory B-factor | X-ray B-factor | Hydrophobicity |
---|---|---|---|---|---|---|
β-barrel | 3,938 | 18.8 ± 4.6 | 0.62 ± 0.13 | 0.32 ± 0.16 | 0.41 ± 0.17 | 0.50 ± 0.09 |
strand β1 | 1,085 | 17.8 ± 4.8 | 0.59 ± 0.15 | 0.34 ± 0.16 | 0.44 ± 0.18 | 0.49 ± 0.10 |
strand β2 | 720 | 19.7 ± 4.6 | 0.64 ± 0.15 | 0.31 ± 0.17 | 0.41 ± 0.16 | 0.51 ± 0.09 |
strand β3 | 614 | 19.7 ± 4.5 | 0.64 ± 0.14 | 0.30 ± 0.14 | 0.39 ± 0.15 | 0.50 ± 0.08 |
strand β4 | 804 | 19.0 ± 4.2 | 0.62 ± 0.13 | 0.31 ± 0.15 | 0.40 ± 0.16 | 0.50 ± 0.09 |
strand β5 | 715 | 18.7 ± 4.4 | 0.61 ± 0.15 | 0.32 ± 0.17 | 0.39 ± 0.17 | 0.49 ± 0.09 |
βOB | 588 | 14.4 ± 3.9 | 0.48 ± 0.13 | 0.46 ± 0.16 | 0.52 ± 0.21 | 0.44 ± 0.10 |
accessory 2° | 1,369 | 14.7 ± 5.0 | 0.46 ± 0.15 | 0.54 ± 0.26 | 0.49 ± 0.22 | 0.44 ± 0.10 |
non-2° | 5,023 | 12.5 ± 5.0 | 0.42 ± 0.17 | 0.57 ± 0.27 | 0.56 ± 0.24 | 0.43 ± 0.10 |
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Guardino, K.M.; Sheftic, S.R.; Slattery, R.E.; Alexandrescu, A.T. Relative Stabilities of Conserved and Non-Conserved Structures in the OB-Fold Superfamily. Int. J. Mol. Sci. 2009, 10, 2412-2430. https://doi.org/10.3390/ijms10052412
Guardino KM, Sheftic SR, Slattery RE, Alexandrescu AT. Relative Stabilities of Conserved and Non-Conserved Structures in the OB-Fold Superfamily. International Journal of Molecular Sciences. 2009; 10(5):2412-2430. https://doi.org/10.3390/ijms10052412
Chicago/Turabian StyleGuardino, Kaitlyn M., Sarah R. Sheftic, Robert E. Slattery, and Andrei T. Alexandrescu. 2009. "Relative Stabilities of Conserved and Non-Conserved Structures in the OB-Fold Superfamily" International Journal of Molecular Sciences 10, no. 5: 2412-2430. https://doi.org/10.3390/ijms10052412
APA StyleGuardino, K. M., Sheftic, S. R., Slattery, R. E., & Alexandrescu, A. T. (2009). Relative Stabilities of Conserved and Non-Conserved Structures in the OB-Fold Superfamily. International Journal of Molecular Sciences, 10(5), 2412-2430. https://doi.org/10.3390/ijms10052412