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Int. J. Mol. Sci. 2010, 11(11), 4297-4308; doi:10.3390/ijms11114297
Article

Antioxidative Peptides Derived from Enzyme Hydrolysis of Bone Collagen after Microwave Assisted Acid Pre-Treatment and Nitrogen Protection

1,2
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1,2,* , 1
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1,2
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1 School of Food Science and Technology, Jiangnan University, Wuxi 214122, China 2 State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
* Author to whom correspondence should be addressed.
Received: 18 August 2010 / Revised: 21 October 2010 / Accepted: 21 October 2010 / Published: 1 November 2010
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

This study focused on the preparation method of antioxidant peptides by enzymatic hydrolysis of bone collagen after microwave assisted acid pre-treatment and nitrogen protection. Phosphoric acid showed the highest ability of hydrolysis among the four other acids tested (hydrochloric acid, sulfuric acid and/or citric acid). The highest degree of hydrolysis (DH) was 9.5% using 4 mol/L phosphoric acid with a ratio of 1:6 under a microwave intensity of 510 W for 240 s. Neutral proteinase gave higher DH among the four protease tested (Acid protease, neutral protease, Alcalase and papain), with an optimum condition of: (1) ratio of enzyme and substrate, 4760 U/g; (2) concentration of substrate, 4%; (3) reaction temperature, 55 °C and (4) pH 7.0. At 4 h, DH increased significantly (P < 0.01) under nitrogen protection compared with normal microwave assisted acid pre-treatment hydrolysis conditions. The antioxidant ability of the hydrolysate increased and reached its maximum value at 3 h; however DH decreased dramatically after 3 h. Microwave assisted acid pre-treatment and nitrogen protection could be a quick preparatory method for hydrolyzing bone collagen.
Keywords: bone collagen; nitrogen protection; microwave assisted acid pre-treatment; enzyme hydrolysis; antioxidant activity bone collagen; nitrogen protection; microwave assisted acid pre-treatment; enzyme hydrolysis; antioxidant activity
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Lin, Y.-J.; Le, G.-W.; Wang, J.-Y.; Li, Y.-X.; Shi, Y.-H.; Sun, J. Antioxidative Peptides Derived from Enzyme Hydrolysis of Bone Collagen after Microwave Assisted Acid Pre-Treatment and Nitrogen Protection. Int. J. Mol. Sci. 2010, 11, 4297-4308.

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