Isolation and Characterization of Microsatellite Loci in Pistacia weinmannifolia (Anacardiaceae)
Abstract
:1. Introduction
2. Results and Discussion
3. Experimental Section
3.1. Isolation of Microsatellite Loci
3.2. Detection of Polymorphism
3.3. Data Analysis
4. Conclusions
Acknowledgments
References
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Locus | Primer Sequence (5′–3′) | Repeat Motif | S (bp) | Ta(°C) | No. |
---|---|---|---|---|---|
PW001 | F: 5′-AATGAGTGGAGAAGGGAAGG-3′ R: 5′-AGCAACTGTTCGCTACCCAG-3′ | (AG)13 | 107 | 59 | JN695599 |
PW002 | F: 5′-CGAAAGATAATCAAGCTAGA-3′ R: 5′-CAACAAGGATGGACCAACAC-3′ | (TC)4CC(TC)5 | 147 | 55 | JN695600 |
PW008 | F: 5′-ATCTTGAATCCTCCCACTAT-3′ R: 5′-ACAACCAAGTCACAGATAGC-3′ | (AC)9(TC)5 | 140 | 52 | JN695601 |
PW014 | F: 5′-ATGCCTTTAGCAACTGAAGT-3′ R: 5′-AGTAGAGATGTATCCATGCC-3′ | (AT)7AG(TG)6 | 178 | 54 | JN695602 |
PW021 | F: 5′-GCAGAAAACCAATGAAAAGC-3′ R: 5′-ACAACCAAGTCACAGATAGC-3′ | (AC)8 | 206 | 52 | JN695603 |
PW039 | F: 5′-GCTGACTTTAGACTATTGAA-3′ R: 5′-TCATCTCTCGTTTGTGGGAC-3′ | (AG)16 | 129 | 54 | JN695605 |
PW047 | F: 5′-AGCCTTGTGTCTGGTTTTAC-3′ R: 5′TTACAACCTTCAAACTTTAT-3′ | (TC)13 | 132 | 49 | JN695606 |
PW056 | F: 5′-AGGTGGTAACAGTCAAGTCG-3′ R: 5′-CAGACAACCAATGAGAAGCA-3′ | (AG)16 | 237 | 57 | JN695607 |
PW058 | F: 5′-GAAAGCCAAGCAAAGCAACA-3′ R: 5′-GGTGGAGCACAGTAACAGCA-3′ | (TC)15 | 114 | 57 | JN695609 |
PW060 | F: 5′-CTCGAAAACCCTAATAACTT-3′ R: 5′-CATAACACCACTCACCAGGC-3′ | (TG)7 | 119 | 55 | JN695610 |
PW061 | F: 5′-GCCACTTTTGTTCATTTCAT-3′ R: 5′-AACTCCAATTAGCTCTACAG-3′ | (AG)13 | 148 | 50 | JN695611 |
PW062 | F: 5′-AGAGAATGAATGGGTAAAAG3′ R: 5′-CATCTTGGGTCCTCCTACTA3′ | (AG)9 | 120 | 53 | JN695612 |
PW081 | F: 5′-GAGGGTGTGTAAGTGTTAGG3′ R: 5′-AAAAGCCACTGGTAGCACTG3′ | (AG)15 | 155 | 52 | JN695614 |
PW088 | F: 5′-GTTACAACCAAGTCGCAGAT-3′ R: 5′-ACCTTGAATCCTCCCACTAT-3′ | (AG)14(TG)9 | 159 | 57 | JN695615 |
WX (N = 12) | XC (N = 12) | CA | |||||
---|---|---|---|---|---|---|---|
Locus | Na | He | Ho | Na | He | Ho | |
PW001 | 2 | 0.464 | 0.083 | 2 | 0.391 | 0.333 | −/− |
PW002 | 4 | 0.663 | 0.25 | 1 | 0.000 | 0.000 | c/− |
PW008 | 4 | 0.663 | 0.333 | 5 | 0.721 | 0.667 | c/− |
PW014 | 2 | 0.290 | 0.000 | 3 | 0.554 | 0.500 | −/m |
PW021 | 3 | 0.540 | 0.250 | 4 | 0.533 | 0.500 | −/− |
PW039 | 2 | 0.228 | 0.250 | 6 | 0.764 | 0.750 | −/− |
PW047 | 3 | 0.353 | 0.250 | 3 | 0.554 | 0.417 | c/m |
PW056 | 5 | 0.739 | 0.833 | 9 | 0.906 | 0.933 | −/− |
PW058 | 5 | 0.757 | 0.833 | 4 | 0.656 | 0.583 | c/− |
PW060 | 3 | 0.518 | 0.417 | 3 | 0.467 | 0.083 | c/m |
PW061 | 2 | 0.4891 | 0.600 | 4 | 0.344 | 0.083 | c/m |
PW062 | 2 | 0.344 | 0.250 | 2 | 0.649 | 0.667 | c/− |
PW081 | 4 | 0.583 | 0.250 | 4 | 0.685 | 0.750 | c/− |
PW088 | 4 | 0.663 | 0.417 | 5 | 0.779 | 0.667 | c/− |
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Chen, S.; Wu, X.; Ji, Y.; Yang, J. Isolation and Characterization of Microsatellite Loci in Pistacia weinmannifolia (Anacardiaceae). Int. J. Mol. Sci. 2011, 12, 7818-7823. https://doi.org/10.3390/ijms12117818
Chen S, Wu X, Ji Y, Yang J. Isolation and Characterization of Microsatellite Loci in Pistacia weinmannifolia (Anacardiaceae). International Journal of Molecular Sciences. 2011; 12(11):7818-7823. https://doi.org/10.3390/ijms12117818
Chicago/Turabian StyleChen, Shaotian, Xiayu Wu, Yunheng Ji, and Junbo Yang. 2011. "Isolation and Characterization of Microsatellite Loci in Pistacia weinmannifolia (Anacardiaceae)" International Journal of Molecular Sciences 12, no. 11: 7818-7823. https://doi.org/10.3390/ijms12117818