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Int. J. Mol. Sci. 2012, 13(11), 14251-14261; doi:10.3390/ijms131114251

Effect of β-Cyclodextrin Complexation on Solubility and Enzymatic Conversion of Naringin

1
Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, 100 Shizi Road, Nanjing 210028, Jiangsu, China
2
Nanjing University of Chinese Medicine, Nanjing 210046, Jiangsu, China
*
Author to whom correspondence should be addressed.
Received: 11 September 2012 / Revised: 16 October 2012 / Accepted: 29 October 2012 / Published: 5 November 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

In the present paper, the effect of β-cyclodextrin (β-CD) inclusion complexation on the solubility and enzymatic hydrolysis of naringin was investigated. The inclusion complex of naringin/β-CD at the molar ratio of 1:1 was obtained by the dropping method and was characterized by differential scanning calorimetry. The solubility of naringin complexes in water at 37 ± 0.1 °C was 15 times greater than that of free naringin. Snailase-involved hydrolysis conditions were tested for the bioconversion of naringin into naringenin using the univariate experimental design. Naringin can be transformed into naringenin by snailase-involved hydrolysis. The optimum conditions for enzymatic hydrolysis were determined as follows: pH 5.0, temperature 37 °C, ratio of snailase/substrate 0.8, substrate concentration 20 mg·mL−1, and reaction time 12 h. Under the optimum conditions, the transforming rate of naringenin from naringin for inclusion complexes and free naringin was 98.7% and 56.2% respectively, suggesting that β-CD complexation can improve the aqueous solubility and consequently the enzymatic hydrolysis rate of naringin. View Full-Text
Keywords: β-cyclodextrin; inclusion complex; enzymolysis; naringin; naringenin β-cyclodextrin; inclusion complex; enzymolysis; naringin; naringenin
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Cui, L.; Zhang, Z.-H.; Sun, E.; Jia, X.-B. Effect of β-Cyclodextrin Complexation on Solubility and Enzymatic Conversion of Naringin. Int. J. Mol. Sci. 2012, 13, 14251-14261.

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