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Int. J. Mol. Sci. 2012, 13(5), 5441-5453; doi:10.3390/ijms13055441

Extraction Optimization of Water-Extracted Mycelial Polysaccharide from Endophytic Fungus Fusarium oxysporum Dzf17 by Response Surface Methodology

Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China
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Received: 15 March 2012 / Revised: 13 April 2012 / Accepted: 25 April 2012 / Published: 4 May 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Water-extracted mycelial polysaccharide (WPS) from the endophytic fungus Fusarium oxysporum Dzf17 isolated from Dioscorea zingiberensis was found to be an efficient elicitor to enhance diosgenin accumulation in D. zingigerensis cultures, and also demonstrated antioxidant activity. In this study, response surface methodology (RSM) was employed to optimize the extraction process of WPS from F. oxysporum Dzf17 using Box-Behnken design (BBD). The ranges of the factors investigated were 1–3 h for extraction time (X1), 80–100 °C for extraction temperature (X2), and 20–40 (v/w) for ratio of water volume (mL) to raw material weight (g) (X3). The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis. Statistical analysis showed that the polynomial regression model was in good agreement with the experimental results with the determination coefficient (R2) of 0.9978. By solving the regression equation and analyzing the response surface contour plots, the extraction parameters were optimized as 1.7 h for extraction time, 95 °C for extraction temperature, 39 (v/w) for ratio of water volume (mL) to raw material weight (g), and with 2 extractions. The maximum value (10.862%) of WPS yield was obtained when the WPS extraction process was conducted under the optimal conditions.
Keywords: optimization; water-extracted mycelial polysaccharide; extraction; endophytic fungus; Fusarium oxysporum Dzf17; response surface methodology optimization; water-extracted mycelial polysaccharide; extraction; endophytic fungus; Fusarium oxysporum Dzf17; response surface methodology
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Li, P.; Lu, S.; Shan, T.; Mou, Y.; Li, Y.; Sun, W.; Zhou, L. Extraction Optimization of Water-Extracted Mycelial Polysaccharide from Endophytic Fungus Fusarium oxysporum Dzf17 by Response Surface Methodology. Int. J. Mol. Sci. 2012, 13, 5441-5453.

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