Pharmacokinetics and Tissue Distribution Study of Praeruptorin D from Radix Peucedani in Rats by High-Performance Liquid Chromatography (HPLC)
School of Pharmaceutical Science, Shanxi Medical University, No 56, Xinjian Nan Road, Taiyuan 030001, Shanxi, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2012, 13(7), 9129-9141; https://doi.org/10.3390/ijms13079129
Submission received: 18 June 2012
/
Revised: 16 July 2012
/
Accepted: 17 July 2012
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Published: 20 July 2012
(This article belongs to the Section Biochemistry)
Abstract
:Praeruptorin D (PD), a major pyranocoumarin isolated from Radix Peucedani, exhibited antitumor and anti-inflammatory activities. The aim of this study was to investigate the pharmacokinetics and tissue distribution of PD in rats following intravenous (i.v.) administration. The levels of PD in plasma and tissues were measured by a simple and sensitive reversed-phase high-performance liquid chromatography (HPLC) method. The biosamples were treated by liquid-liquid extraction (LLE) with methyl tert-butyl ether (MTBE) and osthole was used as the internal standard (IS). The chromatographic separation was accomplished on a reversed-phase C18 column using methanol-water (75:25, v/v) as mobile phase at a flow rate of 0.8 mL/min and ultraviolet detection wave length was set at 323 nm. The results demonstrate that this method has excellent specificity, linearity, precision, accuracy and recovery. The pharmacokinetic study found that PD fitted well into a two-compartment model with a fast distribution phase and a relative slow elimination phase. Tissue distribution showed that the highest concentration was observed in the lung, followed by heart, liver and kidney. Furthermore, PD can also be detected in the brain, which indicated that PD could cross the blood-brain barrier after i.v. administration.
1. Introduction
Radix Peucedani (Baihua Qianhu in Chinese), the dried roots of Peucedanum praeruptorum DUNN (Umbelliferae), is a well-known traditional Chinese medicinal herb which was officially listed in the Chinese Pharmacopoeia [1]. It has been widely used in China for centuries in the treatment of coughs with thick sputum and dyspnea, upper respiratory infections, and nonproductive cough [2]. Phytochemical investigations reveal that coumarins are widely distributed in this plant [3–6]. Among them, angular-type pyranocoumarins (seselins) were regarded as the principal components responsible for the main pharmacological activities. Isolation of pyranocoumarins (praeruptorins A, B, C and D) from this herb was first reported by Chen et al. [3]. Praeruptorin A (PA) was found to be racemic to praeruptorin C (PC), and praeruptorin B (PB) to praeruptorin D (PD, Figure 1A), respectively. Previous studies indicated that PA exhibited a variety of biological activities including anticancer [7–9], vasodilatation [10], reversing multidrug resistance (MDR) [11,12] and calcium antagonistic action [13,14], etc. Moreover, the pharmacokinetics, tissue distribution, metabolism and excretion of PA have been well documented [15–19]. On the other hand, PD, as a PA analogue, has also drawn extensive attention in recent years since it was found to possess some other specific pharmacological effects such as inhibiting tumor promoter induced phenomenon in vitro [20] and potent anti-inflammatory action in addition to the above-mentioned similar activities [21].
Analytical techniques applied to the quantification of PD from herbal medicines included thin-layer chromatography (TLC) [1] and high-performance liquid chromatography (HPLC) with UV detection [22]. Recently, a liquid chromatography coupled with mass spectrometry (HPLC-MS/MS) method was reported to investigate the metabolism of PD in vitro [23]. With the growing significance of a potential beneficial role of PD in human health, there is an increasing demand for investigating its pharmacokinetics in vivo. However, to our knowledge, there has been no research on the pharmacokinetics and tissue distribution in rat after administration of PD. Nowadays, the HPLC-UV method has been commonly used to determine drug contents in complex biological samples. In the present paper, a rapid and sensitive HPLC-UV method was developed and validated to determine PD in rat plasma and tissue homogenates. The method was successfully applied to pharmacokinetics and tissue distribution study after i.v. administration of PD to healthy rats.
2. Results and Discussion
2.1. Preparation of Plasma and Tissue Samples
Biosample preparation was performed using a liquid-liquid extraction (LLE) and different extraction solvents such as ethyl acetate, chloroform, hexane, diethyl ether, and methyl tert-butyl ether (MTBE) were investigated. Finally, MTBE was found to be optimal, because it can produce clean chromatograms for plasma and tissues and yielded the highest recovery for the analytes.
2.2. Method Validation
2.2.1. Specificity
The representative chromatograms for determination of PD in plasma and tissues (liver and lung were chosen as representative tissues) are shown in Figure 2A–C. The retention time of IS was about 7.9 min and PD was 15.2 min. It was indicated that analytes were well separated and no interferences were detected from endogenous substances or metabolites.
2.2.2. Linearity of Calibration Curve and Lower Limit of Quantification
The calibration curves showed good linearity over the concentration range of 0.0512–52.2 μg/mL in rat plasma and tissue homogenates with a correlation coefficient (R2) larger than 0.997. Typical linear regression equations, correlation coefficients and linear ranges of PD in plasma and each tissue were listed in Table 1. The current assay offered an LLOQ of 0.0512 μg/mL in plasma and tissue samples. The limits were sufficient for studies of pharmacokinetics and tissue distribution following a single i.v. administration of PD.
2.2.3. Precision and Accuracy
The precision and accuracy of the method were summarized in Table 2. For all the samples spiked with analytes at three concentration levels, the RSD% of both intra-day and inter-day precision was below 11.25%, and the accuracy was within the range of −11.03 to 10.19%. The results demonstrated that the method is accurate and reproducible for determination of PD in rat plasma and tissues.
2.2.4. Recovery and Stability
The extraction recoveries of PD ranged from 82.07% to 88.63% in plasma and tissue samples (Table 2), while the recovery of IS was above 80% (data not shown). These data indicated the biosample preparation procedure was satisfied and can achieve the acceptable extraction recovery.
The stability tests were designed to cover the anticipated conditions that the samples may experience. The results are summarized in Table 3, which showed that PD remained stable in above three conditions.
2.3. Pharmacokinetics of PD in Rats
The mean plasma concentration-time profiles of PD in rats following i.v. administration at doses of 10 and 20 mg/kg are shown in Figure 3 and the corresponding pharmacokinetic parameters are summarized in Table 4. It was found that the data were best fitted with a two-compartment model. PD had a fast distribution phase (t1/2α, 0.119–0.130 h) followed by a relative slow elimination phase (t1/2β, 2.408–2.640 h) and could be detected until 480 min post dosing using the analytical method described above. A dose proportionality study indicated that there is good correlation between AUC and dose. Significant difference in AUC between the two doses was observed (p < 0.05). On the other hand, there was no significant difference in systemic clearance (CLs) at two dose levels, suggesting that PD may have linear pharmacokinetic characteristics in rats within the dose ranges tested [24].
Furthermore, we also tried to conduct pharmacokinetics study of PD given to rats by oral route. However, the concentration of PD in plasma was too low to be detected even the oral dose was up to 200 mg/kg, which indicated that PD might have a poor absorption from gastrointestinal tract in rat.
2.4. Tissue Distribution Study
The tissue distribution of PD after i.v. administration of 20 mg/kg in rats at 0.5, 1.0, 2.0, 4.0 and 8.0 h was presented in Figure 4. At 1.0 h after administration of PD to rats, the highest level of PD was observed in all collected tissues. Even at 8.0 h, PD was still detectable in all tissues, but obvious downward trend. The results indicated that the PD underwent a rapid and wide distribution in tissues within the time course examined. The highest tissue concentrations were found in the lung, followed by heart, liver and kidney, which implied that the distribution of PD depended on the blood flow or perfusion rate of the organ. The high affinity in lung of PD confirms that PD was a major bioactive component in Radix Peucedani which has good curative effect on respiratory diseases in traditional Chinese medicine. In addition, PD could also be detected in brain homogenate, suggesting that PD can efficiently cross the blood-brain barrier.
3. Experimental Section
3.1. Materials and Reagents
PD was isolated from ethanol extract of the roots of Radix Peucedani as follows: The dried roots (1 kg) were powdered and extracted with 95% ethanol under reflux for three times (3 × 8 L). The combined extract was evaporated under vacuum at 45 °C. Afterwards, the residue was dissolved in a mixture of H2O-ethyl acetate (1:1) and partitioned, the resulting ethyl acetate fraction was separated by column chomatography (CC) on silica gel using petroleum ether-ethyl acetate (7:1) to give PD (2.8 g). The structure was identified by comparison of its physico-chemical (melting point and optical rotation) and spectroscopic (MS, UV, 1H NMR, 13C NMR and IR) data [3]. Its purity was determined to be over 98.0% by HPLC. Osthole (Figure 1B), the internal standard (IS), was obtained from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). HPLC grade methanol was purchased from Tianjin Concord Technology Co. Ltd. (Tianjin, China). Deionized water was purified by Milli-Q system (Millipore, Bedford, MA, USA). Other chemicals used were of analytical grade.
3.2. Animals
Male Sprague-Dawley (SD) rats (10–12 weeks old, weighing 200–230 g) were obtained from the department of Laboratory Animal Science of Shanxi Medical University (Taiyuan, China). Animal welfare and experimental procedures were strictly in accordance with the related ethics regulations of Shanxi Medical University. All animals were kept in an environmentally controlled breeding room (temperature maintained at about 25 ± 2 °C and with a 12 h light/12 h dark cycle) for at least one week before starting the experiments and fed with standard laboratory food and water ad libitum. Prior to each experiment, the rats were fasted for 12 h with free access to water.
3.3. Instrumentation and Chromatographic Conditions
The HPLC analysis was carried out on an Agilent 1200 series liquid chromatographic system (Agilent Technologies, Santa Clara, CA, USA) equipped with G1311A quaternary pump, G1316A thermostatted column compartment, G1322A vacuum degasser, G1329A auto-sampler, and G1315B diode array detector (DAD). Data acquisition was controlled by an Agilent ChemStation B 3.0 software. Chromatographic separation was accomplished on a Diamonsil C18 (4.6 × 150 mm I.D., 5 μm particle size) analytical column (Dikma Technologies Co. Ltd, Beijing, China). The mobile phase was methanol-water (75:25, v/v) at a flow rate of 0.8 mL/min. Chromatograms were monitored at 323 nm and the column temperature was maintained at 25 °C.
3.4. Calibration Standards and Quality Control (QC) Samples Preparation
Stock solution of PD was prepared in methanol to give a final concentration of 1.024 mg/mL. A series of working solutions were obtained by diluting the PD stock solution with methanol. The stock solution of IS (1.0 mg/mL) was also diluted to concentration 10 μg/mL with methanol as working solution. All solutions were stored at 4 °C until used. Calibration standards of PD were prepared by spiking the appropriate amount of the working solutions into 100 μL drug-free rat plasma or tissue homogenates. The final concentrations of calibration standard samples were 0.0512, 0.256, 0.64, 3.2, 12.8 and 51.2 μg/mL.
Quality control (QC) samples were prepared at low, medium and high concentrations of 0.0512, 3.2, 51.2 μg/mL for plasma and different tissue homogenates in the same manner as the calibration standards.
3.5. General Procedure of Sample Preparation for PD Analysis in Plasma and Tissue Samples
A liquid-liquid extraction (LLE) of PD in biosamples was performed prior to HPLC analysis. Briefly, 50 μL of IS working solution (10 μg/mL) was added to 100 μL of plasma or tissue samples in glass centrifuge tubes. The mixture was extracted twice with 3 mL MTBE by vortexing for 5 min. After centrifugation at 10,000 rpm for 5 min, the upper organic layer was transferred to a clean tube and evaporated to dryness under a gentle stream of nitrogen at 40 °C. The residue was reconstituted in 100 μL of methanol and centrifuged at 10,000 rpm for 5 min. An aliquot (20 μL) of supernatant was injected onto the HPLC system.
3.6. Method Validation
3.6.1. Specificity
Specificity was assessed by analyzing blank plasma and tissue homogenate samples, blank plasma and tissue homogenate samples spiked with PD, and rat plasma and tissue samples after i.v. administration of PD.
3.6.2. Calibration Curves and Lower Limit of Quantification
Calibration standard was prepared as described above in triplicate and analyzed on three consequent days. Calibration curves were constructed by plotting the peak area ratio of PD to IS against the concentration of PD using 1/X2 as weighting factor.
The lower limit of quantification (LLOQ) was defined as the lowest concentration of analyte in a sample which provided a peak area with a signal-to-noise ratio higher than 10.
3.6.3. Precision and Accuracy
Intra-day precision and accuracy were evaluated by analysis of the three QC samples with six determinations per concentration at the same day, whilst the inter-day precision and accuracy were measured over three consecutive days. The precision was defined as the relative standard deviation (RSD%), while accuracy was determined by calculating the percentage deviation observed in the analysis of QC samples and expressed by relative error (RE%). The accepted criteria for the data were that the precision and accuracy should not exceed 15%, except at the LLOQ where it should not exceed 20%.
3.6.4. Extraction Recovery and Stability
The extraction recoveries of PD were determined at low, medium and high level of QC samples. Recoveries were calculated by comparing the observed peak area ratios in biosamples to those non-processed standard solutions at the same concentrations. The recovery of IS was determined in the same way at the concentration of 10 μg/mL.
The stability of PD in plasma and tissue was determined under different storage or handling conditions. Short-term stability was assessed by analyzing QC samples kept at ambient temperature for 8 h. Freeze-thaw stability was evaluated at three consecutive freeze-thaw cycles. Long-term stability was studied by assaying samples following a period of 2 weeks of storage at −70 °C.
3.7. In Vivo Pharmacokinetic Study
Twelve rats were randomly assigned to two groups for pharmacokinetic investigation (n = 6 per group). PD was administered by i.v. injection via the lateral tail vein at the dose of 10 and 20 mg/kg, respectively. At the time points of 0 (pre-dose), 5, 15, 30, 45, 60, 90, 120, 240 and 480 min post injection, blood samples (0.5 mL) were collected in heparinized tubes from the orbital vein, and then centrifuged at 10,000 rpm for 5 min to obtain plasma. The plasma was stored at −70 °C prior to analysis by HPLC.
3.8. Tissue Distribution Study
For tissue distribution study, thirty rats were divided into five groups (n = 6 per group) randomly and PD was administered intravenously through the tail vein at a dose of 20 mg/kg. After injection, the rats were sacrificed at 0.5, 1.0, 2.0, 4.0 and 8.0 h following administration, and the tissue specimens including lung, liver, heart, spleen, stomach, small intestine, brain, thymus, muscle, fat and kidney were collected. Tissue samples were rinsed in saline and blotted dry with filter paper, and then weighed for wet weight and homogenized in ice-cold physiological saline solution (500 mg/mL). The obtained tissue homogenates were stored at −70 °C until analysis performed using the procedure described above.
3.9. Statistical Analysis
The pharmacokinetic parameters were calculated using the 3P97 software (Chinese Pharmacology Society: Beijing, China, 1987). An appropriate pharmacokinetic model was chosen based on the lowest Akaike’s information criterion (AIC) value, lowest weighted squared residuals, lowest standard errors of the fitting parameters, and dispersion of the residual under equal weight scheme [25–27]. All the data were expressed as the mean ± standard deviation and the levels of statistical significance were assessed using Student t test.
4. Conclusions
In conclusion, we have developed a simple, rapid and sensitive method for the quantitative determination of PD in biological samples including plasma and tissues. The achieved pharmacokinetics and tissue distribution results may be useful for further study of the bioactive mechanism of PD.
Acknowledgments
This study was financially supported by the National Natural Science Foundation of China (No. 81101687), the Natural Science Foundation of Shanxi Province (No. 2008021048), Program for the Top Science and Technology Innovation Teams of Higher Learning Institutions of Shanxi and by the Program for the Top Young and Middle-aged Innovative Talents of Higher Learning Institutions of Shanxi Province.
References
- The State Pharmacopoeia Commission of P.R. China, Pharmacopoeia of the People’s Republic of China; Chemical Industry Press: Beijing, China, 2010; Volume 1, p. 248.
- Chang, H.M.; But, P.P.H.; Yao, S.C.; Wang, L.L.; Yeung, S.C.S. Pharmacology and Applications of Chinese Materia Medica; World Scientific Publisher: Singapore, 2001; Volume 2, p. 905. [Google Scholar]
- Chen, Z.X.; Huang, B.S.; She, Q.L.; Zeng, G.F. The chemical constituents of Bai-Hua-Qian-Hu, the root of Peucedanum praeruptorum Dunn. (Umbelliferae)—Four new coumarins. Acta Pharm. Sin 1979, 14, 486–496. [Google Scholar]
- Takata, M.; Shibata, S.; Okuyama, T. Structures of angular pyranocoumarins of Bai-Hua Qian-Hu, the root of Peucedanum praeruptorum. Planta Med 1990, 56, 307–311. [Google Scholar]
- Chang, H.T.; Okada, Y.; Ma, T.J.; Okuyama, T.; Tu, P.F. Two new coumarin glycosides from Peucedanum praeruptorum. J. Asian Nat. Prod. Res 2008, 10, 577–581. [Google Scholar]
- Ishii, H.; Okada, Y.; Baba, M.; Okuyama, T. Studies of coumarins from the Chinese drug Qianhu, XXVII: Structure of a new simple coum arin glycoside from Bai-Hua Qianhu, Peucedanum praeruptorum. Chem. Pharm. Bull 2008, 56, 1349–1351. [Google Scholar]
- Fong, W.F.; Zhang, J.X.; Wu, J.Y.; Tse, K.W.; Wang, C.; Cheung, H.Y.; Yang, M.S. Pyranocoumarin(±)-4′-O-acetyl-3′-O-angeloyl-cis-khellactone induces mitochondrial-dependent apoptosis in HL-60 cells. Planta Med 2004, 70, 489–495. [Google Scholar]
- Liang, T.G.; Yue, W.Y.; Li, Q.S. Chemopreventive effects of Peucedanum praeruptorum Dunn and its major constituents on SGC7901 gastric cancer cells. Molecules 2010, 15, 8060–8071. [Google Scholar]
- Mizuno, A.; Okada, Y.; Nishino, H.; Okuyama, T. Anti-cancer activity of coumarins from Peucedanum praeruptum on second stage cancer. J. Tradit. Med 1994, 11, 220–224. [Google Scholar]
- Zhao, N.C.; Jin, W.B.; Zhang, X.H.; Guan, F.L.; Sun, Y.B.; Adachi, H.; Okuyama, T. Relaxant effects of pyranocoumarin compounds isolated from a Chinese medical plant, Bai-Hua Qian-Hu, on isolated rabbit tracheas and pulmonary arteries. Biol. Pharm. Bull 1999, 22, 984–987. [Google Scholar]
- Shen, X.L.; Chen, G.Y.; Zhu, G.Y.; Fong, W.F. (±)-3′-O,4′-O-dicynnamoyl-cis-khellactone, a derivative of (±)-praeruptorin A, resverses P-glycoprotein mediated multidrug resistance in cancer cells. Bioorg. Med. Chem 2006, 14, 7138–7145. [Google Scholar]
- Wu, J.Y.; Fong, W.F.; Zhang, J.X.; Leung, C.H.; Kwong, H.L.; Yang, M.S.; Li, D.; Cheung, H.Y. Reversal of multidrug resistance in cancer cells by pyranocoumarins isolated from Radix Peucedani. Eur. J. Pharmacol 2003, 473, 9–17. [Google Scholar]
- Li, J.M.; Chang, T.H.; Sun, X.D.; Hao, L.Y.; Wang, Y.P.; Yu, Y.F.; Zhang, K.Y. Effect of dl-praeruptorin A on calcium current in ventricular cells of guinea pig. Acta Pharmacol. Sin 1994, 15, 525–527. [Google Scholar]
- Feng, W.Y.; Li, J.M.; Zhang, K.Y. Effect of Pd Ia on elctrophysiological properties of guinea pig ventricular cell (In Chinese). Chin. Pharm. J 1998, 33, 660–662. [Google Scholar]
- Zhang, Z.; Liu, Y.Y.; Su, M.Q.; Liang, X.F.; Wang, W.F.; Zhu, X. Pharmacokinetics, tissue distribution and excretion study of dl-praeruptorin A of Peucedanum praeruptorum in rats by liquid chromatography tandem mass spectrometry. Phytomedicine 2011, 18, 527–532. [Google Scholar]
- Ruan, H.; Zhang, Z.; Zhu, X. Liquid chromatography tandem mass spectrometry pharmacokinetic study of DL-praeruptorin A in rat plasma. Biomed. Chromatogr 2010, 24, 1193–1198. [Google Scholar]
- Jing, W.H.; Song, Y.L.; Yan, R.; Bi, H.C.; Li, P.T.; Wang, Y.T. Transport and metabolism of (±)-praeruptorin A in Caco-2 cell monolayers. Xenobiotica 2011, 41, 71–81. [Google Scholar]
- Ruan, H.; Zhang, Z.; Liang, X.F.; Fu, Y.; Su, M.Q.; Liu, Q.L.; Wang, X.M.; Zhu, X. Metabolism of dl-praeruptorin A in rat liver microsomes using HPLC-electrospray ionization tandem mass spectrometry. Arch. Pharm. Res 2011, 34, 1311–1321. [Google Scholar]
- Song, Y.L.; Jing, W.H.; Zhao, H.Y.; Yan, R.; Li, P.T.; Wang, Y.T. Stereoselective metabolism of (±)-praeruptorin A, a calcium channel blocker from Peucedani Radix, in pooled liver microsomes of rats and humans. Xenobiotica 2012, 42, 231–237. [Google Scholar]
- Nishino, H.; Okuyama, T.; Takata, M.; Shibata, S.; Tokuda, H.; Takayasu, J.; Hasegawa, T.; Nishino, A.; Ueyama, H.; Iwashima, A. Studies on the anti-tumor-promoting activity of naturally occurring substances. IV. Pd-II [(+)anomalin, (+)praeruptorin B], a seselin-type coumarin, inhibits the promotion of skin tumor formation by 12-O-tetradecanoylphorbol-13-acetate in 7,12-dimethylbenz[a]anthracene-initiated mice. Carcinogenesis 1990, 11, 1557–1561. [Google Scholar]
- Yu, P.J.; Jin, H.; Zhang, J.Y.; Wang, G.F.; Li, J.R.; Zhu, Z.G.; Tian, Y.X.; Wu, S.Y.; Xu, W.; Zhang, J.J.; Wu, S.G. Pyranocoumarins isolated from Peucedanum praeruptorum Dunn suppress lipopolysaccharide-induced inflammatory response in murine macrophages through inhibition of NF-κB and STAT3 activation. Inflammation 2012, 35, 967–977. [Google Scholar]
- Wu, W.; Chen, J.; Liu, S.; Yu, N.; Fang, C.; Zhang, Q. Simultaneous determination of three coumarin constituents in roots of Peucedanum praeruptorum by RP-HPLC (In Chinese). Chin. J. Chin. Mater. Med 2009, 34, 1121–1123. [Google Scholar]
- Song, Y.L.; Yan, R.; Jing, W.H.; Zhao, H.Y.; Wang, Y.T. Characterization of metabolism of (+)-praeruptorin B and (+)-praeruptorin E in human and rat liver microsomes by liquid chromatography coupled with ion trap mass spectrometry and time-of-flight mass spectrometry. Rapid Commun. Mass Spectrom 2011, 25, 719–730. [Google Scholar]
- Zhang, W.D.; Zhang, C.; Liu, R.H.; Li, H.L.; Zhang, J.T.; Mao, C.; Moran, S.; Chen, C.L. Preclinical pharmacokinetics and tissue distribution of a natural cardioprotective agent astragaloside IV in rats and dogs. Life Sci 2006, 79, 808–815. [Google Scholar]
- Chen, C.L.; Tai, H.L.; Zhu, D.M.; Uckun, F.M. Pharmacokinetic features and metabolism of calphostin C, a naturally occurring perylenequinone with antileukemic activity. Pharm. Res 1999, 16, 1003–1009. [Google Scholar]
- Chen, C.L.; Rawwas, J.; Sorrell, A.; Eddy, L.; Uckun, F.M. Bioavailability and pharmacokinetic features of etoposide in childhood acute lymphoblastic leukemia patients. Leuk. Lymphoma 2001, 42, 317–327. [Google Scholar]
- Chen, C.L.; Sangiah, S.; Bourne, D.W.; Roder, J.D.; Chen, H.; Alavi, F.K.; Clarke, C.R.; Garrison, G.L.; Berlin, K.D.; Couch, K.M.; et al. Metabolism and metabolite pharmacokinetics of BRB-I-28, a class Ib antiarrhythmic agent. Eur. J. Drug Metab. Pharmacokinet 1995, 20, 151–161. [Google Scholar]
Figure 1.
Chemical structures of praeruptorin D (PD) (A) and Osthole (IS) (B).
Figure 2.
Representative chromatograms of plasma (A), liver (B) and lung (C). 1. blank plasma or tissues (liver and lung); 2. blank plasma or tissues (liver and lung) spiked with PD and IS; 3. plasma or tissues (liver and lung) sample at 60 min following i.v. administration of PD at a single dose of 10 mg/kg.
Figure 2.
Representative chromatograms of plasma (A), liver (B) and lung (C). 1. blank plasma or tissues (liver and lung); 2. blank plasma or tissues (liver and lung) spiked with PD and IS; 3. plasma or tissues (liver and lung) sample at 60 min following i.v. administration of PD at a single dose of 10 mg/kg.
Figure 3.
Plasma concentration-time profiles of PD following i.v. administration in rats at doses of 10 and 20 mg/kg (n = 6).
Figure 3.
Plasma concentration-time profiles of PD following i.v. administration in rats at doses of 10 and 20 mg/kg (n = 6).
Figure 4.
Tissue distribution of PD at times 0.5, 1.0, 2.0, 4.0 and 8.0 h after i.v. injection of 20 mg/kg in rats (n = 6).
Figure 4.
Tissue distribution of PD at times 0.5, 1.0, 2.0, 4.0 and 8.0 h after i.v. injection of 20 mg/kg in rats (n = 6).
| Biosamples | Calibration curves | R2 | Linear range (μg/mL) |
|---|---|---|---|
| Plasma | Y = 0.0876X + 0.0016 | 0.9991 | 0.0512–51.2 |
| Lung | Y = 0.1074X + 0.0054 | 0.9999 | 0.0512–51.2 |
| Liver | Y = 0.1426X + 0.0812 | 0.9984 | 0.0512–51.2 |
| Heart | Y = 0.1314X + 0.0094 | 0.9989 | 0.0512–51.2 |
| Spleen | Y = 0.1381X + 0.0017 | 0.9993 | 0.0512–51.2 |
| Stomach | Y = 0.1318X + 0.0142 | 0.9981 | 0.0512–51.2 |
| Small intestine | Y = 0.1315X + 0.0072 | 0.9973 | 0.0512–51.2 |
| Brain | Y = 0.1417X + 0.0114 | 0.9988 | 0.0512–51.2 |
| Thymus | Y = 0.1381X + 0.0020 | 0.9994 | 0.0512–51.2 |
| Muscle | Y = 0.1493X + 0.0147 | 0.9982 | 0.0512–51.2 |
| Fat | Y = 0.1517X + 0.0039 | 0.9979 | 0.0512–51.2 |
| Kidney | Y = 0.1359X + 0.0018 | 0.9991 | 0.0512–51.2 |
Table 2.
Accuracy, precision and recovery of the method used for determination of PD in rat plasma and tissue samples (n = 6).
| Biosamples | Concentration (μg/mL) | Intra-day | Inter-day | Recovery | ||
|---|---|---|---|---|---|---|
| Precision (RSD%) | Accuracy (RE%) | Precision (RSD%) | Accuracy (RE%) | Mean ± SD (%) | ||
| Plasma | 0.0512 | 8.80 | 9.79 | 11.25 | −10.56 | 84.00 ± 5.88 |
| 3.2 | 5.43 | −8.94 | 6.20 | 8.01 | 83.12 ± 4.06 | |
| 51.2 | 2.91 | −3.62 | 4.07 | 5.98 | 88.63 ± 6.75 | |
| Liver | 0.0512 | 7.29 | −8.51 | 9.62 | −11.03 | 86.56 ± 5.31 |
| 3.2 | 8.47 | −8.48 | 9.97 | −5.44 | 84.51 ± 5.64 | |
| 51.2 | 5.56 | 6.33 | 5.56 | 3.07 | 83.63 ± 6.93 | |
| Lung | 0.0512 | 6.90 | 9.14 | 5.94 | 10.19 | 84.35 ± 5.20 |
| 3.2 | 6.62 | −5.75 | 8.90 | 7.85 | 85.12 ± 4.17 | |
| 51.2 | 5.88 | 6.87 | 10.52 | 7.19 | 82.07 ± 6.42 | |
| Biosamples | Concentration (μg/mL) | Accuracy (RE%) | ||
|---|---|---|---|---|
| Short-term stability | Freeze-thaw stability | Long term stability | ||
| Plasma | 0.0512 | −11.76 | 10.28 | 9.30 |
| 3.2 | −5.22 | −8.75 | −7.04 | |
| 51.2 | 4.43 | −7.11 | 8.89 | |
| Liver | 0.0512 | −8.05 | 5.29 | −8.57 |
| 3.2 | 7.57 | −6.45 | −10.81 | |
| 51.2 | 5.81 | 7.76 | 6.06 | |
| Lung | 0.0512 | −11.35 | 9.03 | −7.15 |
| 3.2 | −9.76 | 7.05 | 6.92 | |
| 51.2 | 8.13 | 5.02 | −4.25 | |
Table 4.
The main pharmacokinetic parameters of PD after i.v. administration (n = 6) t1/2α: distribution phase half-life; t1/2β:elimination phase; AUC0-∞: Area under the concentration-time curve from zero up to infinite time; CLs: systemic clearance.
| Parameter | Unit | Dose | |
|---|---|---|---|
| 10 mg/kg | 20 mg/kg | ||
| t1/2α | h | 0.119 ± 0.036 | 0.130 ± 0.045 |
| t1/2β | h | 2.408 ± 0.409 | 2.640 ± 0.612 |
| AUC0-∞ | mg·h/L | 18.145 ± 6.265 | 40.790 ± 11.746 * |
| CLs | L/h/kg | 0.576 ± 0.193 | 0.513 ± 0.185 |
*p < 0.05, compared with the value of 10 mg/kg dose.
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Liang, T.; Yue, W.; Du, X.; Ren, L.; Li, Q. Pharmacokinetics and Tissue Distribution Study of Praeruptorin D from Radix Peucedani in Rats by High-Performance Liquid Chromatography (HPLC). Int. J. Mol. Sci. 2012, 13, 9129-9141. https://doi.org/10.3390/ijms13079129
AMA Style
Liang T, Yue W, Du X, Ren L, Li Q. Pharmacokinetics and Tissue Distribution Study of Praeruptorin D from Radix Peucedani in Rats by High-Performance Liquid Chromatography (HPLC). International Journal of Molecular Sciences. 2012; 13(7):9129-9141. https://doi.org/10.3390/ijms13079129
Chicago/Turabian StyleLiang, Taigang, Wenyan Yue, Xue Du, Luhui Ren, and Qingshan Li. 2012. "Pharmacokinetics and Tissue Distribution Study of Praeruptorin D from Radix Peucedani in Rats by High-Performance Liquid Chromatography (HPLC)" International Journal of Molecular Sciences 13, no. 7: 9129-9141. https://doi.org/10.3390/ijms13079129
