Shear Stress Inhibits Apoptosis of Ischemic Brain Microvascular Endothelial Cells
AbstractAs a therapeutic strategy for ischemic stroke, to restore or increase cerebral blood flow (CBF) is the most fundamental option. Laminar shear stress (LS), as an important force generated by CBF, mainly acts on brain microvascular endothelial cells (BMECs). In order to study whether LS was a protective factor in stroke, we investigated LS-intervented ischemic apoptosis of rat BMECs (rBMECs) through PE Annexin V/7-AAD, JC-1 and Hoechst 33258 staining to observe the membranous, mitochondrial and nuclear dysfunction. Real-time PCR and western blot were also used to test the gene and protein expressions of Tie-2, Bcl-2 and Akt, which were respectively related to maintain membranous, mitochondrial and nuclear norm. The results showed that LS could be a helpful stimulus for ischemic rBMECs survival. Simultaneously, membranous, mitochondrial and nuclear regulation played an important role in this process. View Full-Text
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Tian, S.; Bai, Y.; Yang, L.; Wang, X.; Wu, Y.; Jia, J.; Zhu, Y.; Cheng, Y.; Zhang, P.; Wu, J.; Wang, N.; Xia, G.; Liao, H.; Zhang, Y.; Shen, X.; Yu, H.; Hu, Y. Shear Stress Inhibits Apoptosis of Ischemic Brain Microvascular Endothelial Cells. Int. J. Mol. Sci. 2013, 14, 1412-1427.
Tian S, Bai Y, Yang L, Wang X, Wu Y, Jia J, Zhu Y, Cheng Y, Zhang P, Wu J, Wang N, Xia G, Liao H, Zhang Y, Shen X, Yu H, Hu Y. Shear Stress Inhibits Apoptosis of Ischemic Brain Microvascular Endothelial Cells. International Journal of Molecular Sciences. 2013; 14(1):1412-1427.Chicago/Turabian Style
Tian, Shan; Bai, Yulong; Yang, Lin; Wang, Xinggang; Wu, Yi; Jia, Jie; Zhu, Yulian; Cheng, Yong; Zhang, Pengyue; Wu, Junfa; Wang, Nianhong; Xia, Guang; Liao, Hua; Zhang, Yuling; Shen, Xiafeng; Yu, Huixian; Hu, Yongshan. 2013. "Shear Stress Inhibits Apoptosis of Ischemic Brain Microvascular Endothelial Cells." Int. J. Mol. Sci. 14, no. 1: 1412-1427.