Int. J. Mol. Sci. 2013, 14(1), 1952-1963; doi:10.3390/ijms14011952
pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells
1
Graduate School of Environmental Science, Hokkaido University, Sapporo 060-0810, Japan
2
Research Institute for Electronic Science (RIES), Hokkaido University, Sapporo 001-0020, Japan
3
Faculty of Advanced Life Science, Hokkaido University, Sapporo 001-0021, Japan
*
Authors to whom correspondence should be addressed.
Received: 21 November 2012 / Revised: 27 December 2012 / Accepted: 9 January 2013 / Published: 18 January 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
Abstract
We have studied physiological parameters in a living cell using fluorescence lifetime imaging of endogenous chromophores. In this study, pH dependence of the fluorescence lifetime of flavin adenine dinucleotide (FAD), that is a significant cofactor exhibiting autofluorescence, has been investigated in buffer solution and in cells. The fluorescence lifetime of FAD remained unchanged with pH 5 to 9 in solution. However, the fluorescence lifetime in HeLa cells was found to decrease with increasing intracellular pH, suggesting that pH in a single cell can be estimated from the fluorescence lifetime imaging of FAD without adding exogenous fluorescent probes.Keywords:
FAD; autofluorescence; living cell; fluorescence lifetime imaging; intracellular pH; fluorescence decay
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Islam, M.S.; Honma, M.; Nakabayashi, T.; Kinjo, M.; Ohta, N. pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells. Int. J. Mol. Sci. 2013, 14, 1952-1963.
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