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pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells
AbstractWe have studied physiological parameters in a living cell using fluorescence lifetime imaging of endogenous chromophores. In this study, pH dependence of the fluorescence lifetime of flavin adenine dinucleotide (FAD), that is a significant cofactor exhibiting autofluorescence, has been investigated in buffer solution and in cells. The fluorescence lifetime of FAD remained unchanged with pH 5 to 9 in solution. However, the fluorescence lifetime in HeLa cells was found to decrease with increasing intracellular pH, suggesting that pH in a single cell can be estimated from the fluorescence lifetime imaging of FAD without adding exogenous fluorescent probes.
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Islam, M.S.; Honma, M.; Nakabayashi, T.; Kinjo, M.; Ohta, N. pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells. Int. J. Mol. Sci. 2013, 14, 1952-1963.View more citation formats
Islam MS, Honma M, Nakabayashi T, Kinjo M, Ohta N. pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells. International Journal of Molecular Sciences. 2013; 14(1):1952-1963.Chicago/Turabian Style
Islam, Md. S.; Honma, Masato; Nakabayashi, Takakazu; Kinjo, Masataka; Ohta, Nobuhiro. 2013. "pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells." Int. J. Mol. Sci. 14, no. 1: 1952-1963.
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