Next Article in Journal
The Ribonucleoprotein Csr Network
Previous Article in Journal
Isolation, Expression, and Characterization of a Hydroperoxide Lyase Gene from Cucumber
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2013, 14(11), 22102-22116; doi:10.3390/ijms141122102

SEPT12-Microtubule Complexes Are Required for Sperm Head and Tail Formation

1
Department of Obstetrics & Gynaecology, National Cheng Kung University, No. 1, University Road, Tainan City 701, Taiwan
2
Graduate Institute of Basic Medicine, Fu Jen Catholic University, No. 510, Zhongzheng Road, Xinzhuang District, New Taipei City 242, Taiwan
3
Graduate Institute of Basic Medical Sciences, National Cheng Kung University, No. 1, University Road, Tainan City 701, Taiwan
4
Research Center for Emerging Viral Infections, Chang Gung University, No. 259 Wen-Hwa 1st Road, Kwei-Shan Taoyuan 333, Taiwan
5
Laboratory Animal Center, National Taiwan University College of Medicine, No. 1, Sec. 4, Roosevelt Road, Taipei 106, Taiwan
6
Department of Biological Sciences and Technology, National University of Tainan, No. 33, Sec. 2, Shulin Street, West Central District, Tainan City 700, Taiwan
7
Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University, National Taiwan University Hospital, No. 1, Sec. 4, Roosevelt Road, Taipei 106, Taiwan
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 6 September 2013 / Revised: 26 September 2013 / Accepted: 26 September 2013 / Published: 7 November 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
View Full-Text   |   Download PDF [2561 KB, uploaded 19 June 2014]   |  

Abstract

The septin gene belongs to a highly conserved family of polymerizing GTP-binding cytoskeletal proteins. SEPTs perform cytoskeletal remodeling, cell polarity, mitosis, and vesicle trafficking by interacting with various cytoskeletons. Our previous studies have indicated that SEPTIN12+/+/+/− chimeras with a SEPTIN12 mutant allele were infertile. Spermatozoa from the vas deferens of chimeric mice indicated an abnormal sperm morphology, decreased sperm count, and immotile sperm. Mutations and genetic variants of SEPTIN12 in infertility cases also caused oligozoospermia and teratozoospermia. We suggest that a loss of SEPT12 affects the biological function of microtublin functions and causes spermiogenesis defects. In the cell model, SEPT12 interacts with α- and β-tubulins by co-immunoprecipitation (co-IP). To determine the precise localization and interactions between SEPT12 and α- and β-tubulins in vivo, we created SEPTIN12-transgene mice. We demonstrate how SEPT12 interacts and co-localizes with α- and β-tubulins during spermiogenesis in these mice. By using shRNA, the loss of SEPT12 transcripts disrupts α- and β-tubulin organization. In addition, losing or decreasing SEPT12 disturbs the morphogenesis of sperm heads and the elongation of sperm tails, the steps of which are coordinated and constructed by α- and β-tubulins, in SEPTIN12+/+/+/− chimeras. In this study, we discovered that the SEPTIN12-microtubule complexes are critical for sperm formation during spermiogenesis. View Full-Text
Keywords: spermiogenesis; SEPT12; microtubules spermiogenesis; SEPT12; microtubules
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Kuo, P.-L.; Chiang, H.-S.; Wang, Y.-Y.; Kuo, Y.-C.; Chen, M.-F.; Yu, I.-S.; Teng, Y.-N.; Lin, S.-W.; Lin, Y.-H. SEPT12-Microtubule Complexes Are Required for Sperm Head and Tail Formation. Int. J. Mol. Sci. 2013, 14, 22102-22116.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top