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Int. J. Mol. Sci. 2013, 14(3), 4613-4628; doi:10.3390/ijms14034613

Covalent Immobilization of Bacillus licheniformis γ-Glutamyl Transpeptidase on Aldehyde-Functionalized Magnetic Nanoparticles

1
Department of Applied Chemistry, National Chiayi University, 300 Syuefu Road, Chiayi City 60004, Taiwan
2
Department of Life Sciences and Institute of Molecular Biology, National Chung Cheng University, Chiayi County 621, Taiwan
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 15 January 2013 / Revised: 20 February 2013 / Accepted: 21 February 2013 / Published: 26 February 2013
(This article belongs to the Special Issue Magnetic Nanoparticles 2013)
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Abstract

This work presents the synthesis and use of surface-modified iron oxide nanoparticles for the covalent immobilization of Bacillus licheniformis γ-glutamyl transpeptidase (BlGGT). Magnetic nanoparticles were prepared by an alkaline solution of divalent and trivalent iron ions, and they were subsequently treated with 3-aminopropyltriethoxysilane (APES) to obtain the aminosilane-coated nanoparticles. The functional group on the particle surface and the amino group of BlGGT was then cross-linked using glutaraldehyde as the coupling reagent. The loading capacity of the prepared nanoparticles for BlGGT was 34.2 mg/g support, corresponding to 52.4% recovery of the initial activity. Monographs of transmission electron microscopy revealed that the synthesized nanoparticles had a mean diameter of 15.1 ± 3.7 nm, and the covalent cross-linking of the enzyme did not significantly change their particle size. Fourier transform infrared spectroscopy confirmed the immobilization of BlGGT on the magnetic nanoparticles. The chemical and kinetic behaviors of immobilized BlGGT are mostly consistent with those of the free enzyme. The immobilized enzyme could be recycled ten times with 36.2% retention of the initial activity and had a comparable stability respective to free enzyme during the storage period of 30 days. Collectively, the straightforward synthesis of aldehyde-functionalized nanoparticles and the efficiency of enzyme immobilization offer wide perspectives for the practical use of surface-bound BlGGT. View Full-Text
Keywords: Bacillus licheniformis; γ-glutamyl transpeptidase; magnetic nanoparticle; 3-aminopropyltriethoxysilane; covalent immobilization Bacillus licheniformis; γ-glutamyl transpeptidase; magnetic nanoparticle; 3-aminopropyltriethoxysilane; covalent immobilization
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Chen, Y.-Y.; Tsai, M.-G.; Chi, M.-C.; Wang, T.-F.; Lin, L.-L. Covalent Immobilization of Bacillus licheniformis γ-Glutamyl Transpeptidase on Aldehyde-Functionalized Magnetic Nanoparticles. Int. J. Mol. Sci. 2013, 14, 4613-4628.

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