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Int. J. Mol. Sci. 2013, 14(3), 5723-5733; doi:10.3390/ijms14035723
Article

9G DNAChip Technology: Self-Assembled Monolayer (SAM) of ssDNA for Ultra-Sensitive Detection of Biomarkers

1
,
2
,
2
,
1
 and
1,*
1 Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 200-702, Korea 2 Biometrix Technology, Inc. 202 BioVenture Plaza, Chuncheon 200-161, Korea
* Author to whom correspondence should be addressed.
Received: 8 December 2012 / Revised: 1 February 2013 / Accepted: 4 March 2013 / Published: 12 March 2013
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Abstract

A 9G DNAChip obtained by allowing the formation of a self-assembled monolayer (SAM) of oligonucleotides appended with nine consecutive guanines on the chip surface has been applied in the detection of biomarkers. Using a 9G DNAChip, biomarker in the concentration range of 4 pg/mL to 40 fg/mL can be easily differentiated in the buffer matrix. Moreover, it is the first time that a biomarker with a concentration of 40 fg/mL has been detected in a mixture of proteins without use of any signal amplification technique.
Keywords: self-assembly; SAM of ssDNA; biomarker; 9G DNAChip; hybridization; C-reactive protein self-assembly; SAM of ssDNA; biomarker; 9G DNAChip; hybridization; C-reactive protein
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Nimse, S.B.; Song, K.-S.; Kim, J.; Sayyed, D.R.; Kim, T. 9G DNAChip Technology: Self-Assembled Monolayer (SAM) of ssDNA for Ultra-Sensitive Detection of Biomarkers. Int. J. Mol. Sci. 2013, 14, 5723-5733.

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