Melatonin Inhibits GnRH-1, GnRH-3 and GnRH Receptor Expression in the Brain of the European Sea Bass, Dicentrarchus labrax
Abstract
:1. Introduction
2. Results
3. Discussion
4. Material and Methods
4.1. Animals
4.2. Experimental Procedure
4.3. Quantitative Real Time PCR Assays in Sea Bass Brain
4.4. Data Analysis
5. Conclusions
Acknowledgements
Conflict of Interest
References
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Gene | Forward primer sequence | Reverse primer sequence | Annealing temperature | GenBank accession no. |
---|---|---|---|---|
GnRH-1 | qPCR-GnRH-1-F: GGTCCTATGGACTGAGTCCAGG | qPCR-GnRH-1-R: TGATTCCTCTGCACAACCTAA | 60 °C | AF224279 |
GnRH-2 | qPCR-GnRH-2-F: GTGTGAGGCAGGAGAATGCA | qPCR-GnRH-2-R: CTGGCTAAGGCATCCAGAATG | 60 °C | AF224281 |
GnRH-3 | qPCR-GnRH-3-F: TGTGGGAGAGCTAGAGGCAAC | qPCR-GnRH-3-R: GTTTGGGCACTCGCCTCTT | 60 °C | AF224280 |
GnRH-R II-1a | qPCR-1a-F: CTCTGGCTATCAATAAGGC | qPCR-1a-R: CTCGGGATGGATGATGGT | 59 °C | AJ419594 |
GnRH-R II-1b | qPCR-1b-F: CTGCTGATGTTCTTGAAACTGG | qPCR-1b-R: GAAGTTCTCTGGCACTGTGATG | 64 °C | AJ606686 |
GnRH-R II-1c | qPCR-1c-F: TGATGGTGGCGTGGACTA | qPCR-1c-R: GAGTAAAGTTTGCTGGATAAG | 59 °C | AJ606684 |
GnRH-R II-2a | qPCR-2a-F: TGACGCTGTATGTCTTCCC | qPCR-2a-R: CATCCGGGCTTTGGGTAT | 59 °C | AJ606683 |
GnRH-R II-2b | qPCR-2b-F: AGACTCTGAAGATGACGGTGGT | qPCR-2b-R: AGTGAAGCGTCTCTTCTCATCC | 64 °C | AJ606685 |
18S | qPCR-18S-F: GCATGGCCGTTCTTAGTTGGT | qPCR-18S-R: GCATGCCGGAGTCTCGTT | 48 °C | AY831388 |
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Servili, A.; Herrera-Pérez, P.; Del Carmen Rendón, M.; Muñoz-Cueto, J.A. Melatonin Inhibits GnRH-1, GnRH-3 and GnRH Receptor Expression in the Brain of the European Sea Bass, Dicentrarchus labrax. Int. J. Mol. Sci. 2013, 14, 7603-7616. https://doi.org/10.3390/ijms14047603
Servili A, Herrera-Pérez P, Del Carmen Rendón M, Muñoz-Cueto JA. Melatonin Inhibits GnRH-1, GnRH-3 and GnRH Receptor Expression in the Brain of the European Sea Bass, Dicentrarchus labrax. International Journal of Molecular Sciences. 2013; 14(4):7603-7616. https://doi.org/10.3390/ijms14047603
Chicago/Turabian StyleServili, Arianna, Patricia Herrera-Pérez, María Del Carmen Rendón, and José Antonio Muñoz-Cueto. 2013. "Melatonin Inhibits GnRH-1, GnRH-3 and GnRH Receptor Expression in the Brain of the European Sea Bass, Dicentrarchus labrax" International Journal of Molecular Sciences 14, no. 4: 7603-7616. https://doi.org/10.3390/ijms14047603