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Int. J. Mol. Sci. 2013, 14(4), 8556-8569; doi:10.3390/ijms14048556
Article

MiR-590-5P Inhibits Growth of HepG2 Cells via Decrease of S100A10 Expression and Inhibition of the Wnt Pathway

1,†
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2,3,†
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4,* , 2
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3
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3
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3
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 and
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1 Department of Geraeology, Yancheng City No.1 People's Hospital, Yancheng 224001, China 2 Department of General Surgery, the First Affiliated Hospital of Soochow University, Suzhou 215006, China 3 Department of Medical Oncology, Yancheng City No.1 People's Hospital, Yancheng 224001, China 4 Department of General Surgery, Yancheng City No.1 People's Hospital, Yancheng 224001, China These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 19 February 2013 / Revised: 8 March 2013 / Accepted: 7 April 2013 / Published: 18 April 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Hepatocellular carcinoma is one of the most common and lethal cancers worldwide, especially in developing countries. In the present study, we found that the expression of a microRNA, miR-590-5P, was down-regulated and S100A10 was up-regulated in six hepatocellular carcinoma cell lines. The reporter gene assay showed that overexpression of miR-590-5P effectively reduced the activity of luciferase expressed by a vector bearing the 3' untranslated region of S100A10 mRNA. Ectopic miR-590-5P overexpression mediated by lentiviral infection decreased expression of S100A10. Infection of Lv-miR-590-5P inhibited cell growth and induced cell cycle G1 arrest in HepG2 cells. In addition, miR-590-5P expression suppressed the expression of Wnt5a, cMyc and cyclin D1, and increased the phosphorylation of β-catenin and expression of Caspase 3, which may contribute to the inhibitory effect of miR-590-5P on cell growth. Taken together, our data suggest that down-regulation of miR-590-5P is involved in hepatocellular carcinoma and the restoration of miR-590-5P can impair the growth of cancer cells, suggesting that miR-590-5P may be a potential target molecule for the therapy of hepatocellular carcinoma.
Keywords: miR-590-5P; S100A10; hepatocellular carcinoma; Wnt pathway; lentiviral system; reporter gene miR-590-5P; S100A10; hepatocellular carcinoma; Wnt pathway; lentiviral system; reporter gene
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Shan, X.; Miao, Y.; Fan, R.; Qian, H.; Chen, P.; Liu, H.; Yan, X.; Li, J.; Zhou, F. MiR-590-5P Inhibits Growth of HepG2 Cells via Decrease of S100A10 Expression and Inhibition of the Wnt Pathway. Int. J. Mol. Sci. 2013, 14, 8556-8569.

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