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Int. J. Mol. Sci. 2013, 14(4), 8625-8637; doi:10.3390/ijms14048625

Single Nucleotide Polymorphisms in the Leptin-a Gene and Associations with Growth Traits in the Orange-Spotted Grouper (Epinephelus coioides)

1
Key Laboratory of Tropical Biology Resources, Ministry of Education, College of Ocean, Hainan University, Haikou 570228, China
2
Sanya Science & Technology Academy of Hainan National Breeding and Multiplication, Sanya 572000, China
3
State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, College of Life Sciences, Sun Yat-Sen (Zhongshan) University, Guangzhou 510275, China
*
Authors to whom correspondence should be addressed.
Received: 7 February 2013 / Revised: 11 April 2013 / Accepted: 16 April 2013 / Published: 22 April 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Leptin is a multifunctional protein involved in processes such as body weight regulation, energy expenditure, fat metabolism, food intake, and appetite regulation. Duplicate leptin genes, leptin-a and leptin-b, were previously detected in the orange-spotted grouper. In this study, we cloned the full-length open reading frame (ORF) of the leptin-a gene in the orange-spotted grouper, searched for polymorphisms, and performed association analyses between these polymorphisms and seven growth traits. Six polymorphisms, consisting of 2 SNPs in intron 1 (c.182T > G, c.183G > T) and 4 SNPs in exon 2 (c.339C > G, c.345C > T, c.447G > A, c.531C > T), were identified and genotyped in 200 individuals. The c.182T > G and c.183G > T polymorphisms showed complete linkage and were analyzed together. Association analyses revealed that the c.182 + 183TG > GT polymorphism was significantly associated with body weight (BWT) and body width (BWH), with the AB (TG/GT) genotype showing positive effects on growth traits. Additionally, the SNP c.447G > A was significantly associated with BWT, BWH, overall length (OL), trunk width (TW), and head length (HL), with the GA genotype displaying positive effects on growth traits. The c.531C > T SNP showed a close association between the TT genotype and decreased growth. Our results demonstrate that several polymorphisms in the leptin-a gene are associated with growth traits and can be used for marker-assisted selection (MAS) in orange-spotted grouper populations. View Full-Text
Keywords: leptin; single nucleotide polymorphisms (SNPs); molecular markers; orange-spotted grouper (Epinephelus coioides); growth traits; association analysis leptin; single nucleotide polymorphisms (SNPs); molecular markers; orange-spotted grouper (Epinephelus coioides); growth traits; association analysis
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Wei, Y.; Huang, H.; Meng, Z.; Zhang, Y.; Luo, J.; Chen, G.; Lin, H. Single Nucleotide Polymorphisms in the Leptin-a Gene and Associations with Growth Traits in the Orange-Spotted Grouper (Epinephelus coioides). Int. J. Mol. Sci. 2013, 14, 8625-8637.

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