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Int. J. Mol. Sci. 2013, 14(5), 9051-9061; doi:10.3390/ijms14059051
Article

IGF-1 Antibody Prolongs the Effective Duration Time of Botulinum Toxin in Decreasing Muscle Strength

1,†,* , 1,†
,
1
,
1
,
2
,
1
 and
1,*
1 Department of Neurology, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China 2 Department of Biomedicine, China National Center for Biotechnology Development, Beijing 100036, China These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 18 February 2013 / Revised: 28 March 2013 / Accepted: 15 April 2013 / Published: 25 April 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Botulinum toxin type-A (Btx-A), a powerful therapeutic tool in various medical specialties, requires repeated injections to maintain its effect. Therefore, novel methods to prolong the effective duration time of Btx-A are highly needed. Rats were assigned to three major groups: control group (n = 30), Btx-A group (n = 30), and IGF-1 Ab groups. IGF-1 Ab groups were composed by sub-groups A1–A5 (each has 25 rats) for the subsequent IGF-1Ab dose-effect study. Muscle strength was determined by a survey system for rat lower limbs nerve and muscle function. Muscle-specific receptor tyrosine kinase (MuSK), Insulin-like growth factor binding protein-5 (IGFBP5), and growth-associated protein, 43-kDa (GAP43) were determined by real-time polymerase chain reactions (PCRs) and Western blot. We found that Btx-A decreased the muscle strength, with a paralysis maintained for 70 days. IGF-1Ab prolonged the effective duration time of Btx-A. Real-time PCRs and Western blot showed that IGF-1Ab delayed the increase of MuSK and IGFBP5 after Btx-A injection, without affecting GAP43. These results indicate that IGF-1Ab might prolong the effective duration time of Btx-A on muscle strength through delaying the increase of MuSK. It would be interesting to determine whether IGF-1Ab can be used as an auxiliary measure to the Btx-A treatment in the future.
Keywords: botulinum toxin; insulin-like growth factor 1; muscle strength; muscle-specific receptor tyrosine kinase botulinum toxin; insulin-like growth factor 1; muscle strength; muscle-specific receptor tyrosine kinase
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Jin, L.; Pan, L.; Liu, W.; Guo, Y.; Zheng, Y.; Guan, Q.; Nie, Z. IGF-1 Antibody Prolongs the Effective Duration Time of Botulinum Toxin in Decreasing Muscle Strength. Int. J. Mol. Sci. 2013, 14, 9051-9061.

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